Rmu_co8430541.1_g000001
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8430541.1
OG0024429
Physical Location & Seq
Forward (+)
135 .. 1472
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8430541.1_g000001.1.cds

Sequence Viewer

Length: 987 bp
atgatgatcatcactctggcctcttcaaatctgagaggaaatgaggtggaaagactatccttgcttgcctttaaatatgaaatattcagtcatcctatgggcatgcttagctcctggaatgattcctccaacttatgtgatgagtggcgagggattacttgcggccgaagacaccacttcaccggcaccatccctcaagaagttggtcgattgtcccagctgcaagtactacacctcggggacaacctgttcagaggttatatccccatcaatatatcacattgttctaatctccaatatcttgttttggctaataacaatcttactggcgaaattccagtagaaattggctcattgttgaagcttcaagaacttactttgcgcaggaacaatttagtaggggaaattcccccttcctttggaaatctttcttctcttgagattctttctatggaaggaaataatctgcaaggtggtctcatgaatagccgctttgggaaattgaaaagcataagagctcttgcattgggtacaaatagattgtctggtaccatccctccctccatatacaacctttcttcaattcaatacacaatatttccaaaacttcaaatctttcataattattttaccggaaaagttcctagtctggcaagagtgtccactctggttcggttggaaattgatgataataatcttggaaatgaggatggtgacttggattttctctcttctctggtcaattgtaccagtttagaacttctggatattagtgggaataaccttctaggagtgctacctgaatctatgatcaatctctcaacaaagctcaaggtaatgagcttaggaaggaatcagctaagcggaagcaatcccattgggattggaaatctcctcaacttgggagttctaagcttcgaggcaaacctattgacaggcaccataccaaattcagaatttgttagcattggttctagtctgttttag

Protein Analysis

328

Amino Acids

35.93

Weight (kDa)

6.08

Isoelectric Point (pI)

32.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024429)

Species Orthologous Gene IDs
malus_domestica MD00G1043900.v1.1
rosa_laevigata RLG00000029274
rosa_multiflora Rmu_co8430541.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 383
Acc65I GGTACC 1 cut(s) 548
AccB1I GGYRCC 3 cut(s) 185, 548, 938
AciI CCGC 3 cut(s) 162, 490, 864
AcoI YGGCCR 1 cut(s) 163
AcsI RAATTY 4 cut(s) 333, 405, 949, 956
AfaI GTAC 4 cut(s) 228, 532, 550, 748
AfiI CCNNNNNNNGG 2 cut(s) 419, 901
AgsI TTSAA 7 cut(s) 27, 361, 368, 505, 582, 587, 611
AjnI CCWGG 1 cut(s) 113
AloI GAACNNNNNNTCC 2 cut(s) 233, 265
AluBI AGCT 8 cut(s) 111, 220, 364, 518, 829, 843, 859, 915
AluI AGCT 8 cut(s) 111, 220, 364, 518, 829, 843, 859, 915
Alw21I GWGCWC 1 cut(s) 520
Alw26I GTCTC 1 cut(s) 482
Ama87I CYCGRG 1 cut(s) 236
AoxI GGCC 2 cut(s) 18, 163
ApeKI GCWGC 1 cut(s) 220
ApoI RAATTY 4 cut(s) 333, 405, 949, 956
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
Asp700I GAANNNNTTC 1 cut(s) 427
Asp718I GGTACC 1 cut(s) 548
AspLEI GCGC 1 cut(s) 384
AsuHPI GGTGA 2 cut(s) 172, 725
AvaI CYCGRG 1 cut(s) 236
BanI GGYRCC 3 cut(s) 185, 548, 938
BanII GRGCYC 1 cut(s) 520
BbsI GAAGAC 1 cut(s) 175
Bbv12I GWGCWC 1 cut(s) 520
BbvI GCAGC 1 cut(s) 207
BccI CCATC 4 cut(s) 197, 275, 560, 704
BciT130I CCWGG 1 cut(s) 115
BclI TGATCA 2 cut(s) 6, 810
BcoDI GTCTC 1 cut(s) 482
BfaI CTAG 3 cut(s) 645, 788, 975
BisI GCNGC 3 cut(s) 163, 221, 490
BlpI GCTNAGC 2 cut(s) 107, 860
BlsI GCNGC 3 cut(s) 164, 222, 491
BmcAI AGTACT 1 cut(s) 228
Bme1390I CCNGG 1 cut(s) 115
BmeT110I CYCGRG 1 cut(s) 236
BmiI GGNNCC 3 cut(s) 187, 550, 940
BmrFI CCNGG 1 cut(s) 115
BpiI GAAGAC 1 cut(s) 175
Bpu10I CCTNAGC 1 cut(s) 844
Bpu1102I GCTNAGC 2 cut(s) 107, 860
BpuEI CTTGAG 3 cut(s) 180, 458, 815
BsaBI GATNNNNATC 2 cut(s) 8, 693
BsaI GGTCTC 1 cut(s) 482
BsaJI CCNNGG 1 cut(s) 235
BsaWI WCCGGW 1 cut(s) 632
BsaXI ACNNNNNCTCC 2 cut(s) 541, 571
Bsc4I CCNNNNNNNGG 2 cut(s) 419, 901
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 3 cut(s) 331, 338, 750
Bse8I GATNNNNATC 2 cut(s) 8, 693
BseBI CCWGG 1 cut(s) 115
BseDI CCNNGG 1 cut(s) 235
BseGI GGATG 4 cut(s) 91, 189, 552, 715
BseJI GATNNNNATC 2 cut(s) 8, 693
BseLI CCNNNNNNNGG 2 cut(s) 419, 901
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 3 cut(s) 331, 338, 750
BseRI GAGGAG 1 cut(s) 884
BseX3I CGGCCG 1 cut(s) 163
BseXI GCAGC 1 cut(s) 207
BseYI CCCAGC 1 cut(s) 216
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 2 cut(s) 20, 165
BshNI GGYRCC 3 cut(s) 185, 548, 938
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 520
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 2 cut(s) 183, 633
BslFI GGGAC 2 cut(s) 199, 254
BslI CCNNNNNNNGG 2 cut(s) 419, 901
BsmAI GTCTC 1 cut(s) 482
BsmFI GGGAC 2 cut(s) 199, 254
BsnI GGCC 2 cut(s) 20, 165
Bso31I GGTCTC 1 cut(s) 482
BsoBI CYCGRG 1 cut(s) 236
Bsp1286I GDGCHC 1 cut(s) 520
Bsp143I GATC 2 cut(s) 6, 810
Bsp1720I GCTNAGC 2 cut(s) 107, 860
BspACI CCGC 3 cut(s) 162, 490, 864
BspANI GGCC 2 cut(s) 20, 165
BspCNI CTCAG 1 cut(s) 24
BspHI TCATGA 1 cut(s) 480
BspLI GGNNCC 3 cut(s) 187, 550, 940
BspT107I GGYRCC 3 cut(s) 185, 548, 938
BspTNI GGTCTC 1 cut(s) 482
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 3 cut(s) 331, 338, 750
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 235
BssMI GATC 2 cut(s) 6, 810
Bst2UI CCWGG 1 cut(s) 115
Bst6I CTCTTC 2 cut(s) 28, 736
BstC8I GCNNGC 2 cut(s) 66, 104
BstDEI CTNAG 5 cut(s) 32, 107, 844, 860, 911
BstF5I GGATG 4 cut(s) 91, 189, 552, 715
BstHHI GCGC 1 cut(s) 384
BstKTI GATC 2 cut(s) 9, 813
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 2 cut(s) 6, 810
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 1 cut(s) 108
BstNI CCWGG 1 cut(s) 115
BstNSI RCATGY 1 cut(s) 106
BstSCI CCNGG 1 cut(s) 113
BstV1I GCAGC 1 cut(s) 207
BstV2I GAAGAC 1 cut(s) 175
BstZI CGGCCG 1 cut(s) 163
BsuRI GGCC 2 cut(s) 20, 165
BtsCI GGATG 4 cut(s) 91, 189, 552, 715
Cac8I GCNNGC 2 cut(s) 66, 104
CciI TCATGA 1 cut(s) 480
CfoI GCGC 1 cut(s) 384
Cfr10I RCCGGY 1 cut(s) 182
Csp6I GTAC 4 cut(s) 227, 531, 549, 747
CviAII CATG 2 cut(s) 103, 481
CviQI GTAC 4 cut(s) 227, 531, 549, 747
DdeI CTNAG 5 cut(s) 32, 107, 844, 860, 911
DpnI GATC 2 cut(s) 8, 812
DpnII GATC 2 cut(s) 6, 810
DraI TTTAAA 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 163
EagI CGGCCG 1 cut(s) 163
Eam1104I CTCTTC 2 cut(s) 28, 736
EarI CTCTTC 2 cut(s) 28, 736
Ecl136II GAGCTC 1 cut(s) 518
EclXI CGGCCG 1 cut(s) 163
Eco24I GRGCYC 1 cut(s) 520
Eco31I GGTCTC 1 cut(s) 482
Eco52I CGGCCG 1 cut(s) 163
Eco53kI GAGCTC 1 cut(s) 518
Eco88I CYCGRG 1 cut(s) 236
EcoICRI GAGCTC 1 cut(s) 518
EcoRII CCWGG 1 cut(s) 113
EcoT38I GRGCYC 1 cut(s) 520
FaeI CATG 2 cut(s) 106, 484
FaqI GGGAC 2 cut(s) 199, 254
FatI CATG 2 cut(s) 102, 480
FbaI TGATCA 2 cut(s) 6, 810
Fnu4HI GCNGC 3 cut(s) 163, 221, 490
FokI GGATG 4 cut(s) 78, 176, 539, 722
FriOI GRGCYC 1 cut(s) 520
Fsp4HI GCNGC 3 cut(s) 163, 221, 490
FspBI CTAG 3 cut(s) 645, 788, 975
FspI TGCGCA 1 cut(s) 383
GlaI GCGC 1 cut(s) 383
GluI GCNGC 3 cut(s) 163, 221, 490
GsaI CCCAGC 1 cut(s) 220
HaeIII GGCC 2 cut(s) 20, 165
HapII CCGG 2 cut(s) 183, 633
HhaI GCGC 1 cut(s) 384
Hin1II CATG 2 cut(s) 106, 484
Hin6I GCGC 1 cut(s) 382
HinP1I GCGC 1 cut(s) 382
HindIII AAGCTT 2 cut(s) 362, 913
HinfI GANTC 4 cut(s) 122, 442, 803, 853
HpaII CCGG 2 cut(s) 183, 633
HphI GGTGA 2 cut(s) 172, 725
Hpy166II GTNNAC 1 cut(s) 663
Hpy188I TCNGA 3 cut(s) 33, 254, 955
Hpy188III TCNNGA 5 cut(s) 197, 368, 437, 481, 764
Hpy8I GTNNAC 1 cut(s) 663
HpyAV CCTTC 4 cut(s) 423, 449, 794, 843
HpyCH4V TGCA 3 cut(s) 223, 469, 524
HpyF10VI GCNNNNNNNGC 1 cut(s) 108
HpyF3I CTNAG 5 cut(s) 32, 107, 844, 860, 911
Hsp92II CATG 2 cut(s) 106, 484
HspAI GCGC 1 cut(s) 382
KpnI GGTACC 1 cut(s) 552
Ksp22I TGATCA 2 cut(s) 6, 810
Kzo9I GATC 2 cut(s) 6, 810
LmnI GCTCC 1 cut(s) 116
Lsp1109I GCAGC 1 cut(s) 207
MaeI CTAG 3 cut(s) 645, 788, 975
MaeIII GTNAC 1 cut(s) 713
MalI GATC 2 cut(s) 8, 812
MboI GATC 2 cut(s) 6, 810
MboII GAAGA 5 cut(s) 15, 180, 423, 570, 723
MfeI CAATTG 1 cut(s) 742
MhlI GDGCHC 1 cut(s) 520
MmeI TCCRAC 2 cut(s) 153, 657
MroXI GAANNNNTTC 1 cut(s) 427
MseI TTAA 1 cut(s) 72
MspA1I CMGCKG 1 cut(s) 220
MspI CCGG 2 cut(s) 183, 633
MspR9I CCNGG 1 cut(s) 115
MunI CAATTG 1 cut(s) 742
MvaI CCWGG 1 cut(s) 115
MwoI GCNNNNNNNGC 1 cut(s) 108
NdeII GATC 2 cut(s) 6, 810
NlaIII CATG 2 cut(s) 106, 484
NlaIV GGNNCC 3 cut(s) 187, 550, 940
NmuCI GTSAC 1 cut(s) 713
NsbI TGCGCA 1 cut(s) 383
NspI RCATGY 1 cut(s) 106
PaeI GCATGC 1 cut(s) 106
PagI TCATGA 1 cut(s) 480
PdmI GAANNNNTTC 1 cut(s) 427
PfeI GAWTC 4 cut(s) 122, 442, 803, 853
PfoI TCCNGGA 1 cut(s) 113
PkrI GCNGC 3 cut(s) 164, 222, 491
Psp124BI GAGCTC 1 cut(s) 520
Psp6I CCWGG 1 cut(s) 113
PspFI CCCAGC 1 cut(s) 216
PspGI CCWGG 1 cut(s) 113
PspN4I GGNNCC 3 cut(s) 187, 550, 940
PvuII CAGCTG 1 cut(s) 220
RsaI GTAC 4 cut(s) 228, 532, 550, 748
RsaNI GTAC 4 cut(s) 227, 531, 549, 747
SacI GAGCTC 1 cut(s) 520
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 3 cut(s) 163, 221, 490
Sau3AI GATC 2 cut(s) 6, 810
ScaI AGTACT 1 cut(s) 228
ScrFI CCNGG 1 cut(s) 115
SduI GDGCHC 1 cut(s) 520
SmlI CTYRAG 3 cut(s) 195, 437, 830
SmoI CTYRAG 3 cut(s) 195, 437, 830
SphI GCATGC 1 cut(s) 106
SsiI CCGC 3 cut(s) 162, 490, 864
SspI AATATT 2 cut(s) 84, 597
SspMI CTAG 3 cut(s) 645, 788, 975
SstI GAGCTC 1 cut(s) 520
StyD4I CCNGG 1 cut(s) 113
TaqI TCGA 2 cut(s) 208, 918
TatI WGTACW 1 cut(s) 226
TauI GCSGC 2 cut(s) 165, 492
TfiI GAWTC 4 cut(s) 122, 442, 803, 853
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TseFI GTSAC 1 cut(s) 713
TseI GCWGC 1 cut(s) 220
Tsp45I GTSAC 1 cut(s) 713
TspDTI ATGAA 3 cut(s) 93, 497, 608
XapI RAATTY 4 cut(s) 333, 405, 949, 956
XceI RCATGY 1 cut(s) 106
XmnI GAANNNNTTC 1 cut(s) 427
XspI CTAG 3 cut(s) 645, 788, 975
ZrmI AGTACT 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.