RLG00000030188

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0022603
Physical Location & Seq
Reverse (-)
57263276 .. 57264130
855 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030188

Sequence Viewer

Length: 543 bp
ATGAGACGTGATGTAGCAAAGAAGAAGTCACAGACTTATTCATGCAAGGAACATAGCGACTGTGTGGATCGTATCATCAATGGTACTTCATCTGGAGGTTACACTTGCCGGTGCACGACAGGCTACGATGAGAACCCATGCGTATCAGATGGTTGCCAAGGTATAAGTATAAGCATGTTTGTCTTACTGGTTGGAATTTTTTGGTTGGATTGTGGAAGGAAGAGAAGGAAGTTCGAACAACTGAAAGAAAAGTACTTCAAAGAAAATGATGGCGAGAAGTTACGAGAAAAACTGGCTAGTCACAAGGGATCTGTCGAGACAGCAGCCAGGATCTTTACCACGGCAGACCTCAAGCAGGCCACAAACAATTACCACAGAGATGGAATCCTTGGTGAAGGAGGTTACGGAGCTGGTTACAAAGGAGTACTTCCGGATAATAAACTGGTAGCCATAAAGAAGTCCAAAATCATGGCCAAGTCACAGACTGATCAGTTTGTTAATGAGGTGGCTGTTCTCTCTCAAATGAACCATAGAAATGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

181

Amino Acids

20.13

Weight (kDa)

9.21

Isoelectric Point (pI)

20.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 124 - 180 2.2e-06 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022603)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0324581
rosa_laevigata RLG00000030188
rosa_roxburghii Rroxscaffold_4G00325370
rosa_wichuraiana Rw1G005310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 430
AclWI GGATC 3 cut(s) 75, 316, 338
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 1 cut(s) 195
AfaI GTAC 3 cut(s) 85, 254, 426
AfiI CCNNNNNNNGG 1 cut(s) 355
AgsI TTSAA 1 cut(s) 259
AjiI CACGTC 1 cut(s) 8
AjnI CCWGG 1 cut(s) 326
AluBI AGCT 1 cut(s) 410
AluI AGCT 1 cut(s) 410
Alw21I GWGCWC 1 cut(s) 116
Alw26I GTCTC 1 cut(s) 311
Alw44I GTGCAC 1 cut(s) 112
AlwI GGATC 3 cut(s) 75, 316, 338
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 2 cut(s) 357, 471
ApaLI GTGCAC 1 cut(s) 112
ApeKI GCWGC 1 cut(s) 323
ApoI RAATTY 1 cut(s) 195
ArsI GACNNNNNNTTYG 2 cut(s) 11, 43
AsuHPI GGTGA 1 cut(s) 404
AsuII TTCGAA 1 cut(s) 234
BaeGI GKGCMC 1 cut(s) 116
BalI TGGCCA 1 cut(s) 473
Bbv12I GWGCWC 1 cut(s) 116
BbvI GCAGC 1 cut(s) 335
BccI CCATC 3 cut(s) 143, 263, 374
BceAI ACGGC 1 cut(s) 357
BciT130I CCWGG 1 cut(s) 328
BclI TGATCA 1 cut(s) 487
BcoDI GTCTC 1 cut(s) 311
BfaI CTAG 1 cut(s) 297
BisI GCNGC 1 cut(s) 324
BlsI GCNGC 1 cut(s) 325
BmcAI AGTACT 2 cut(s) 254, 426
Bme1390I CCNGG 1 cut(s) 328
BmgBI CACGTC 1 cut(s) 8
BmrFI CCNGG 1 cut(s) 328
BpmI CTGGAG 1 cut(s) 114
Bpu14I TTCGAA 1 cut(s) 234
BpuEI CTTGAG 1 cut(s) 335
BsaJI CCNNGG 3 cut(s) 157, 339, 388
BsaWI WCCGGW 1 cut(s) 430
Bsc4I CCNNNNNNNGG 1 cut(s) 355
Bse118I RCCGGY 1 cut(s) 108
Bse1I ACTGG 3 cut(s) 192, 297, 447
BseAI TCCGGA 1 cut(s) 430
BseBI CCWGG 1 cut(s) 328
BseDI CCNNGG 3 cut(s) 157, 339, 388
BseLI CCNNNNNNNGG 1 cut(s) 355
BseNI ACTGG 3 cut(s) 192, 297, 447
BseSI GKGCMC 1 cut(s) 116
BseXI GCAGC 1 cut(s) 335
BshFI GGCC 2 cut(s) 359, 473
BsiHKAI GWGCWC 1 cut(s) 116
BsiSI CCGG 2 cut(s) 109, 431
BslI CCNNNNNNNGG 1 cut(s) 355
BsmAI GTCTC 1 cut(s) 311
BsnI GGCC 2 cut(s) 359, 473
Bsp119I TTCGAA 1 cut(s) 234
Bsp1286I GDGCHC 1 cut(s) 116
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 4 cut(s) 67, 308, 330, 487
BspANI GGCC 2 cut(s) 359, 473
BspEI TCCGGA 1 cut(s) 430
BspPI GGATC 3 cut(s) 75, 316, 338
BspT104I TTCGAA 1 cut(s) 234
BsrFI RCCGGY 1 cut(s) 108
BsrI ACTGG 3 cut(s) 192, 297, 447
BssAI RCCGGY 1 cut(s) 108
BssECI CCNNGG 3 cut(s) 157, 339, 388
BssMI GATC 4 cut(s) 67, 308, 330, 487
BssT1I CCWWGG 2 cut(s) 157, 388
Bst2UI CCWGG 1 cut(s) 328
Bst4CI ACNGT 1 cut(s) 62
Bst6I CTCTTC 1 cut(s) 215
BstBI TTCGAA 1 cut(s) 234
BstC8I GCNNGC 1 cut(s) 357
BstDSI CCRYGG 1 cut(s) 339
BstENI CCTNNNNNAGG 1 cut(s) 353
BstKTI GATC 4 cut(s) 70, 311, 333, 490
BstMAI GTCTC 1 cut(s) 311
BstMBI GATC 4 cut(s) 67, 308, 330, 487
BstMWI GCNNNNNNNGC 1 cut(s) 120
BstNI CCWGG 1 cut(s) 328
BstNSI RCATGY 1 cut(s) 178
BstSCI CCNGG 1 cut(s) 326
BstSLI GKGCMC 1 cut(s) 116
BstV1I GCAGC 1 cut(s) 335
BstX2I RGATCY 2 cut(s) 308, 330
BstXI CCANNNNNNTGG 2 cut(s) 380, 469
BstYI RGATCY 2 cut(s) 308, 330
BsuRI GGCC 2 cut(s) 359, 473
BtgI CCRYGG 1 cut(s) 339
BtrI CACGTC 1 cut(s) 8
Cac8I GCNNGC 1 cut(s) 357
Cfr10I RCCGGY 1 cut(s) 108
Csp6I GTAC 3 cut(s) 84, 253, 425
CviAII CATG 4 cut(s) 42, 138, 175, 469
CviJI RGCY 8 cut(s) 123, 296, 326, 359, 410, 449, 473, 509
CviKI_1 RGCY 8 cut(s) 123, 296, 326, 359, 410, 449, 473, 509
CviQI GTAC 3 cut(s) 84, 253, 425
DpnI GATC 4 cut(s) 69, 310, 332, 489
DpnII GATC 4 cut(s) 67, 308, 330, 487
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
Eco130I CCWWGG 2 cut(s) 157, 388
EcoNI CCTNNNNNAGG 1 cut(s) 353
EcoRII CCWGG 1 cut(s) 326
EcoT14I CCWWGG 2 cut(s) 157, 388
ErhI CCWWGG 2 cut(s) 157, 388
FaeI CATG 4 cut(s) 45, 141, 178, 472
FaiI YATR 9 cut(s) 43, 54, 139, 164, 170, 176, 452, 470, 531
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FatI CATG 4 cut(s) 41, 137, 174, 468
FbaI TGATCA 1 cut(s) 487
Fnu4HI GCNGC 1 cut(s) 324
Fsp4HI GCNGC 1 cut(s) 324
FspBI CTAG 1 cut(s) 297
GluI GCNGC 1 cut(s) 324
GsuI CTGGAG 1 cut(s) 114
HaeIII GGCC 2 cut(s) 359, 473
HapII CCGG 2 cut(s) 109, 431
Hin1II CATG 4 cut(s) 45, 141, 178, 472
HinfI GANTC 1 cut(s) 384
HpaII CCGG 2 cut(s) 109, 431
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 1 cut(s) 114
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 3 cut(s) 93, 316, 431
Hpy8I GTNNAC 1 cut(s) 114
HpyAV CCTTC 3 cut(s) 210, 219, 389
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4IV ACGT 1 cut(s) 7
HpyCH4V TGCA 2 cut(s) 45, 114
HpyF10VI GCNNNNNNNGC 1 cut(s) 120
HpySE526I ACGT 1 cut(s) 7
Hsp92II CATG 4 cut(s) 45, 141, 178, 472
Kpn2I TCCGGA 1 cut(s) 430
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 4 cut(s) 67, 308, 330, 487
LmnI GCTCC 1 cut(s) 407
Lsp1109I GCAGC 1 cut(s) 335
MaeI CTAG 1 cut(s) 297
MaeII ACGT 1 cut(s) 7
MaeIII GTNAC 7 cut(s) 27, 98, 279, 299, 401, 413, 477
MalI GATC 4 cut(s) 69, 310, 332, 489
MboI GATC 4 cut(s) 67, 308, 330, 487
MboII GAAGA 2 cut(s) 34, 232
MflI RGATCY 2 cut(s) 308, 330
MhlI GDGCHC 1 cut(s) 116
MlsI TGGCCA 1 cut(s) 473
MluCI AATT 2 cut(s) 195, 367
MluNI TGGCCA 1 cut(s) 473
MmeI TCCRAC 2 cut(s) 172, 186
MnlI CCTC 4 cut(s) 89, 359, 392, 496
Mox20I TGGCCA 1 cut(s) 473
MroI TCCGGA 1 cut(s) 430
MscI TGGCCA 1 cut(s) 473
MseI TTAA 1 cut(s) 498
MslI CAYNNNNRTG 2 cut(s) 378, 534
Msp20I TGGCCA 1 cut(s) 473
MspI CCGG 2 cut(s) 109, 431
MspR9I CCNGG 1 cut(s) 328
MvaI CCWGG 1 cut(s) 328
MwoI GCNNNNNNNGC 1 cut(s) 120
NdeII GATC 4 cut(s) 67, 308, 330, 487
NlaIII CATG 4 cut(s) 45, 141, 178, 472
NmuCI GTSAC 3 cut(s) 27, 299, 477
NspI RCATGY 1 cut(s) 178
NspV TTCGAA 1 cut(s) 234
PfeI GAWTC 1 cut(s) 384
PkrI GCNGC 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 326
PspGI CCWGG 1 cut(s) 326
PsuI RGATCY 2 cut(s) 308, 330
RsaI GTAC 3 cut(s) 85, 254, 426
RsaNI GTAC 3 cut(s) 84, 253, 425
RseI CAYNNNNRTG 2 cut(s) 378, 534
SaqAI TTAA 1 cut(s) 498
SatI GCNGC 1 cut(s) 324
Sau3AI GATC 4 cut(s) 67, 308, 330, 487
ScaI AGTACT 2 cut(s) 254, 426
ScrFI CCNGG 1 cut(s) 328
SduI GDGCHC 1 cut(s) 116
SetI ASST 7 cut(s) 10, 100, 163, 351, 403, 412, 507
SfuI TTCGAA 1 cut(s) 234
SmiMI CAYNNNNRTG 2 cut(s) 378, 534
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
Sse9I AATT 2 cut(s) 195, 367
SspMI CTAG 1 cut(s) 297
StyD4I CCNGG 1 cut(s) 326
StyI CCWWGG 2 cut(s) 157, 388
TaaI ACNGT 1 cut(s) 62
TaiI ACGT 1 cut(s) 10
TaqI TCGA 2 cut(s) 234, 315
TasI AATT 2 cut(s) 195, 367
TatI WGTACW 2 cut(s) 252, 424
TfiI GAWTC 1 cut(s) 384
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TseFI GTSAC 3 cut(s) 27, 299, 477
TseI GCWGC 1 cut(s) 323
Tsp45I GTSAC 3 cut(s) 27, 299, 477
TspDTI ATGAA 3 cut(s) 30, 78, 539
TspGWI ACGGA 1 cut(s) 420
VneI GTGCAC 1 cut(s) 112
XagI CCTNNNNNAGG 1 cut(s) 353
XapI RAATTY 1 cut(s) 195
XceI RCATGY 1 cut(s) 178
XspI CTAG 1 cut(s) 297
ZrmI AGTACT 2 cut(s) 254, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.