RLG00000032318

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0022776
Physical Location & Seq
Reverse (-)
13476568 .. 13477002
435 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032318

Sequence Viewer

Length: 435 bp
ATGGAGATCCCAAGCTTTTCAATATTAGTCGCAGTAATATCAGAACCCATTCTCAGCGATTTCGTCCAATGCCTTGCAAAACAATCTTCCTCCTCCCCAATCCCAGCAAATGTTCTCAATCAGCAAAACACCACTTTCCAAGATGCTTTGTTGGCTTACACTAGAAACAAGAGAATTTCGACACCCGCAACGCCAAAACCTTGGGCCATTGTGCCCGCTGCGCACGAATCCCACGTCCAAGCAACTGTGATTTGTGCAAGACAGCACAGGTTTCAGCTCCGAATCCGAAGCGGGGGCCACGACTTCGAGGCTCTTTCGTATGTATCGAATGTTCCTTTCGTGATCCTTGACTTGTTTAATCTCCGGTCCGTTAACGTCAATGTCAAGGACGAGAGCGCTTGGGTCCGGTGCTACTATCGGAGAGATTTATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

145

Amino Acids

16.26

Weight (kDa)

8.93

Isoelectric Point (pI)

58.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 69 - 136 4.6e-06 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022776)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0017871
rosa_laevigata RLG00000032317 RLG00000032318
rosa_wichuraiana Rw5G011470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 222
AciI CCGC 3 cut(s) 186, 216, 291
AclWI GGATC 1 cut(s) 337
AcsI RAATTY 1 cut(s) 174
AfeI AGCGCT 1 cut(s) 397
AfiI CCNNNNNNNGG 1 cut(s) 292
AgsI TTSAA 1 cut(s) 21
AjiI CACGTC 1 cut(s) 235
AluBI AGCT 2 cut(s) 15, 277
AluI AGCT 2 cut(s) 15, 277
AlwI GGATC 1 cut(s) 337
Aor51HI AGCGCT 1 cut(s) 397
AoxI GGCC 2 cut(s) 204, 295
ApeKI GCWGC 1 cut(s) 218
ApoI RAATTY 1 cut(s) 174
ArsI GACNNNNNNTTYG 2 cut(s) 219, 251
Asp700I GAANNNNTTC 1 cut(s) 48
AspLEI GCGC 2 cut(s) 223, 398
AspS9I GGNCC 4 cut(s) 204, 295, 366, 403
AvaII GGWCC 2 cut(s) 366, 403
BaeGI GKGCMC 1 cut(s) 216
BbvI GCAGC 1 cut(s) 205
BfaI CTAG 2 cut(s) 162, 433
BfoI RGCGCY 1 cut(s) 399
BisI GCNGC 1 cut(s) 219
BlsI GCNGC 1 cut(s) 220
Bme18I GGWCC 2 cut(s) 366, 403
BmgBI CACGTC 1 cut(s) 235
BmgT120I GGNCC 4 cut(s) 204, 295, 366, 403
BmiI GGNNCC 2 cut(s) 296, 404
BmsI GCATC 1 cut(s) 133
BsaJI CCNNGG 1 cut(s) 200
BsaWI WCCGGW 2 cut(s) 363, 405
Bsc4I CCNNNNNNNGG 1 cut(s) 292
BseDI CCNNGG 1 cut(s) 200
BseLI CCNNNNNNNGG 1 cut(s) 292
BseMII CTCAG 1 cut(s) 67
BseRI GAGGAG 1 cut(s) 82
BseSI GKGCMC 1 cut(s) 216
BseXI GCAGC 1 cut(s) 205
BseYI CCCAGC 1 cut(s) 103
BshFI GGCC 2 cut(s) 206, 297
BsiSI CCGG 2 cut(s) 364, 406
BslI CCNNNNNNNGG 1 cut(s) 292
BsnI GGCC 2 cut(s) 206, 297
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 2 cut(s) 6, 342
BspACI CCGC 3 cut(s) 186, 216, 291
BspANI GGCC 2 cut(s) 206, 297
BspCNI CTCAG 1 cut(s) 66
BspLI GGNNCC 2 cut(s) 296, 404
BspPI GGATC 1 cut(s) 337
BssECI CCNNGG 1 cut(s) 200
BssMI GATC 2 cut(s) 6, 342
BssT1I CCWWGG 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 247
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 1 cut(s) 53
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 2 cut(s) 223, 398
BstKTI GATC 2 cut(s) 9, 345
BstMBI GATC 2 cut(s) 6, 342
BstMWI GCNNNNNNNGC 2 cut(s) 152, 220
BstSLI GKGCMC 1 cut(s) 216
BstV1I GCAGC 1 cut(s) 205
BstX2I RGATCY 1 cut(s) 6
BstXI CCANNNNNNTGG 1 cut(s) 201
BstYI RGATCY 1 cut(s) 6
BsuRI GGCC 2 cut(s) 206, 297
BtrI CACGTC 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 216
CfoI GCGC 2 cut(s) 223, 398
Cfr13I GGNCC 4 cut(s) 204, 295, 366, 403
CpoI CGGWCCG 1 cut(s) 366
CspI CGGWCCG 1 cut(s) 366
CviJI RGCY 6 cut(s) 15, 155, 206, 277, 297, 311
CviKI_1 RGCY 6 cut(s) 15, 155, 206, 277, 297, 311
DdeI CTNAG 1 cut(s) 53
DpnI GATC 2 cut(s) 8, 344
DpnII GATC 2 cut(s) 6, 342
Eco130I CCWWGG 1 cut(s) 200
Eco47I GGWCC 2 cut(s) 366, 403
Eco47III AGCGCT 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 200
FaiI YATR 1 cut(s) 321
FauI CCCGC 3 cut(s) 193, 223, 284
Fnu4HI GCNGC 1 cut(s) 219
Fsp4HI GCNGC 1 cut(s) 219
FspBI CTAG 2 cut(s) 162, 433
FspI TGCGCA 1 cut(s) 222
GlaI GCGC 2 cut(s) 222, 397
GluI GCNGC 1 cut(s) 219
GsaI CCCAGC 1 cut(s) 107
HaeII RGCGCY 1 cut(s) 399
HaeIII GGCC 2 cut(s) 206, 297
HapII CCGG 2 cut(s) 364, 406
HhaI GCGC 2 cut(s) 223, 398
Hin6I GCGC 2 cut(s) 221, 396
HinP1I GCGC 2 cut(s) 221, 396
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 2 cut(s) 227, 282
HpaI GTTAAC 1 cut(s) 373
HpaII CCGG 2 cut(s) 364, 406
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 4 cut(s) 43, 281, 287, 420
Hpy188III TCNNGA 1 cut(s) 340
Hpy8I GTNNAC 1 cut(s) 373
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 2 cut(s) 234, 375
HpyCH4V TGCA 2 cut(s) 77, 257
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 220
HpyF3I CTNAG 1 cut(s) 53
HpySE526I ACGT 2 cut(s) 234, 375
HspAI GCGC 2 cut(s) 221, 396
KspAI GTTAAC 1 cut(s) 373
Kzo9I GATC 2 cut(s) 6, 342
LmnI GCTCC 1 cut(s) 282
LpnPI CCDG 4 cut(s) 117, 253, 377, 419
Lsp1109I GCAGC 1 cut(s) 205
LweI GCATC 1 cut(s) 133
MaeI CTAG 2 cut(s) 162, 433
MaeII ACGT 2 cut(s) 234, 375
MalI GATC 2 cut(s) 8, 344
MboI GATC 2 cut(s) 6, 342
MboII GAAGA 1 cut(s) 78
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 1 cut(s) 216
MluCI AATT 1 cut(s) 174
MnlI CCTC 3 cut(s) 100, 103, 301
MroXI GAANNNNTTC 1 cut(s) 48
MseI TTAA 2 cut(s) 357, 372
MspA1I CMGCKG 1 cut(s) 218
MspI CCGG 2 cut(s) 364, 406
MwoI GCNNNNNNNGC 2 cut(s) 152, 220
NdeII GATC 2 cut(s) 6, 342
NlaIV GGNNCC 2 cut(s) 296, 404
NsbI TGCGCA 1 cut(s) 222
PcsI WCGNNNNNNNCGW 2 cut(s) 231, 323
PdmI GAANNNNTTC 1 cut(s) 48
PfeI GAWTC 2 cut(s) 227, 282
PkrI GCNGC 1 cut(s) 220
PspFI CCCAGC 1 cut(s) 103
PspN4I GGNNCC 2 cut(s) 296, 404
PspPI GGNCC 4 cut(s) 204, 295, 366, 403
PsrI GAACNNNNNNTAC 2 cut(s) 315, 347
PsuI RGATCY 1 cut(s) 6
Rsr2I CGGWCCG 1 cut(s) 366
RsrII CGGWCCG 1 cut(s) 366
SaqAI TTAA 2 cut(s) 357, 372
SatI GCNGC 1 cut(s) 219
Sau3AI GATC 2 cut(s) 6, 342
Sau96I GGNCC 4 cut(s) 204, 295, 366, 403
SduI GDGCHC 1 cut(s) 216
SetI ASST 6 cut(s) 17, 202, 237, 272, 279, 378
SfaNI GCATC 1 cut(s) 133
SinI GGWCC 2 cut(s) 366, 403
Sse9I AATT 1 cut(s) 174
SsiI CCGC 3 cut(s) 186, 216, 291
SspI AATATT 1 cut(s) 24
SspMI CTAG 2 cut(s) 162, 433
StyI CCWWGG 1 cut(s) 200
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 2 cut(s) 237, 378
TaqI TCGA 3 cut(s) 179, 306, 326
TasI AATT 1 cut(s) 174
TfiI GAWTC 2 cut(s) 227, 282
Tru1I TTAA 2 cut(s) 357, 372
Tru9I TTAA 2 cut(s) 357, 372
TseI GCWGC 1 cut(s) 218
TspGWI ACGGA 1 cut(s) 358
VpaK11BI GGWCC 2 cut(s) 366, 403
XapI RAATTY 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 48
XspI CTAG 2 cut(s) 162, 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.