Rw5G011470

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0022776
Physical Location & Seq
Reverse (-)
13122530 .. 13124972
2443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G011470.1

Sequence Viewer

Length: 1203 bp
ATGGATTGGGAAGTGGAAGAGTCCTTTAGATCATACAATGTGAGCTTGACTCCAATGTTGCTTTTTCTTGGGGCAATTGGCGCACTCAATGAAATTGACCCACAAAATGTCGCTGCCGGTGTCGACTTGCACGTAGTAGTCCTTCGACGGGGATCCGATTCAGAGCTTGGCGAAGTAGAGACAGCAATATCAGAACCCATTATCAGCGATTTCGCCCATTGCCTTGCAAAACAATCTTCCTCCTCCCCAATCCCAGCAAATGTTCTCAATCAGCAAAACCCCACTTTCCAAGATGCTTTGTTGGCTTACACTACAAACAAGAGATTTTCGACACCCGCAACTCCAAAACCTTGGGTCATTGTGCCCGCTGCGCACGAATCCCACGTCCAAGCAACTGTGATTTGTGCAAAACAGCACAGGTTTCAGCTCCGAATCCAAAGCGGGGGCCACGACTTCGAGGCTCTTTCGTATGTATCGAATGTTCCTTTCGTGATCCTTGACTTGTTTAATCTCCGGTCCATTAACGTCAATGTCAAGGACTTGAGAGCTGGGGTCGAGTCCGGTGCTACTATCGGACAGATTTATTCTAGAATTGTGGAGAAAAGTAACGTCCACGGGTTTCCGGCTGCTGCTTGCCCTGGTGTTGGTGCTGGTGGTCACTTCAGCGGGGGTGGGTATGGCAACTTGATGAGAAAATACGGGCTCGCAGCAGATAACATTGTGGATGCCAAAATAGTGGATGTAAATGGTACCATTCTTGATCGGCAGTCAATGGGAGAAGACCTATTTTGGGCCATTAGAGGTGGTGCTACGGGAAGCTTTGGTGTCATTTTATCATGGAAGATCAAACTAGTCCGAGTTCCGAAAAAAGTGACGGTTTTCAGAGTAAAAAAAACTCTGGAACAAAGTGCAACAGATGTTATTTACAAGTGGCAATTTGTTGGACCGGAGCTTCCTCCAGAGATTTTCATCAGAGCAATGCCTCGAGTGAAAAAAAAAACCACCAAGGGCACCAACACTGTGGAAGTGACATTCATTGGTTTCTTCTTGGGACGAAGTCATAAGCTTGTTCCATTGTTAACTAAGAGGTTCCCTGAATTTGGTTTGCAAAGTAAAGATAGCATTGAAATGAGTTGGATAAACTCCACCATATTCTGGGCTGATTTCCCACTAGGAACTCGATCCCACTTCTGTTCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

400

Amino Acids

43.94

Weight (kDa)

8.92

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 119 - 253 4.5e-21 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022776)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0017871
rosa_laevigata RLG00000032317 RLG00000032318
rosa_wichuraiana Rw5G011470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 372
Acc65I GGTACC 1 cut(s) 749
AccB1I GGYRCC 2 cut(s) 749, 1010
AccB7I CCANNNNNTGG 1 cut(s) 1155
AccI GTMKAC 1 cut(s) 123
AciI CCGC 4 cut(s) 336, 366, 441, 666
AclWI GGATC 4 cut(s) 147, 160, 487, 1176
AcsI RAATTY 1 cut(s) 1097
AcuI CTGAAG 1 cut(s) 646
AfaI GTAC 1 cut(s) 751
AfiI CCNNNNNNNGG 6 cut(s) 148, 442, 644, 790, 1100, 1155
AgsI TTSAA 1 cut(s) 1127
AhlI ACTAGT 1 cut(s) 850
AjiI CACGTC 1 cut(s) 385
AjnI CCWGG 1 cut(s) 637
AluBI AGCT 7 cut(s) 45, 166, 427, 548, 819, 952, 1066
AluI AGCT 7 cut(s) 45, 166, 427, 548, 819, 952, 1066
Alw26I GTCTC 1 cut(s) 173
AlwI GGATC 4 cut(s) 147, 160, 487, 1176
Ama87I CYCGRG 1 cut(s) 984
AoxI GGCC 2 cut(s) 445, 792
ApeKI GCWGC 5 cut(s) 113, 368, 626, 629, 707
ApoI RAATTY 1 cut(s) 1097
ArsI GACNNNNNNTTYG 2 cut(s) 369, 401
Asp718I GGTACC 1 cut(s) 749
AspLEI GCGC 2 cut(s) 83, 373
AspS9I GGNCC 4 cut(s) 445, 516, 792, 944
AvaI CYCGRG 1 cut(s) 984
AvaII GGWCC 2 cut(s) 516, 944
BaeGI GKGCMC 2 cut(s) 366, 1013
BamHI GGATCC 1 cut(s) 152
BanI GGYRCC 2 cut(s) 749, 1010
BanII GRGCYC 1 cut(s) 705
BarI GAAGNNNNNNTAC 2 cut(s) 126, 158
BbsI GAAGAC 1 cut(s) 786
BbvI GCAGC 5 cut(s) 100, 355, 613, 616, 719
BcgI CGANNNNNNTGC 2 cut(s) 545, 579
BciT130I CCWGG 1 cut(s) 639
BcoDI GTCTC 1 cut(s) 173
BcuI ACTAGT 1 cut(s) 850
BfaI CTAG 3 cut(s) 588, 851, 1172
BisI GCNGC 5 cut(s) 114, 369, 627, 630, 708
BlsI GCNGC 5 cut(s) 115, 370, 628, 631, 709
Bme1390I CCNGG 1 cut(s) 639
Bme18I GGWCC 2 cut(s) 516, 944
BmeT110I CYCGRG 1 cut(s) 984
BmgBI CACGTC 1 cut(s) 385
BmgT120I GGNCC 4 cut(s) 445, 516, 792, 944
BmiI GGNNCC 5 cut(s) 154, 446, 751, 1012, 1091
BmrFI CCNGG 1 cut(s) 639
BmsI GCATC 2 cut(s) 283, 715
BpiI GAAGAC 1 cut(s) 786
BplI GAGNNNNNCTC 2 cut(s) 34, 66
BpmI CTGGAG 1 cut(s) 942
BpuEI CTTGAG 1 cut(s) 562
BsaAI YACGTR 1 cut(s) 133
BsaBI GATNNNNATC 1 cut(s) 968
BsaJI CCNNGG 4 cut(s) 350, 613, 637, 1005
BsaWI WCCGGW 3 cut(s) 513, 560, 946
Bsc4I CCNNNNNNNGG 6 cut(s) 148, 442, 644, 790, 1100, 1155
Bse118I RCCGGY 1 cut(s) 116
Bse3DI GCAATG 2 cut(s) 217, 984
Bse8I GATNNNNATC 1 cut(s) 968
BseBI CCWGG 1 cut(s) 639
BseDI CCNNGG 4 cut(s) 350, 613, 637, 1005
BseGI GGATG 2 cut(s) 730, 745
BseJI GATNNNNATC 1 cut(s) 968
BseLI CCNNNNNNNGG 6 cut(s) 148, 442, 644, 790, 1100, 1155
BseMI GCAATG 2 cut(s) 217, 984
BseRI GAGGAG 1 cut(s) 232
BseSI GKGCMC 2 cut(s) 366, 1013
BseXI GCAGC 5 cut(s) 100, 355, 613, 616, 719
BseYI CCCAGC 2 cut(s) 253, 548
BshFI GGCC 2 cut(s) 447, 794
BshNI GGYRCC 2 cut(s) 749, 1010
BsiHKCI CYCGRG 1 cut(s) 984
BsiSI CCGG 5 cut(s) 117, 514, 561, 623, 947
BslFI GGGAC 1 cut(s) 1065
BslI CCNNNNNNNGG 6 cut(s) 148, 442, 644, 790, 1100, 1155
BsmAI GTCTC 1 cut(s) 173
BsmFI GGGAC 1 cut(s) 1065
BsnI GGCC 2 cut(s) 447, 794
BsoBI CYCGRG 1 cut(s) 984
Bsp1286I GDGCHC 3 cut(s) 366, 705, 1013
Bsp143I GATC 6 cut(s) 29, 152, 492, 760, 843, 1181
BspACI CCGC 4 cut(s) 336, 366, 441, 666
BspANI GGCC 2 cut(s) 447, 794
BspLI GGNNCC 5 cut(s) 154, 446, 751, 1012, 1091
BspPI GGATC 4 cut(s) 147, 160, 487, 1176
BspT107I GGYRCC 2 cut(s) 749, 1010
BsrDI GCAATG 2 cut(s) 217, 984
BsrFI RCCGGY 1 cut(s) 116
BssAI RCCGGY 1 cut(s) 116
BssECI CCNNGG 4 cut(s) 350, 613, 637, 1005
BssMI GATC 6 cut(s) 29, 152, 492, 760, 843, 1181
BssT1I CCWWGG 2 cut(s) 350, 1005
Bst2UI CCWGG 1 cut(s) 639
Bst4CI ACNGT 3 cut(s) 397, 877, 1021
Bst6I CTCTTC 1 cut(s) 12
BstBAI YACGTR 1 cut(s) 133
BstC8I GCNNGC 3 cut(s) 366, 634, 705
BstDEI CTNAG 1 cut(s) 1083
BstDSI CCRYGG 1 cut(s) 613
BstF5I GGATG 2 cut(s) 730, 745
BstHHI GCGC 2 cut(s) 83, 373
BstKTI GATC 6 cut(s) 32, 155, 495, 763, 846, 1184
BstMAI GTCTC 1 cut(s) 173
BstMBI GATC 6 cut(s) 29, 152, 492, 760, 843, 1181
BstMWI GCNNNNNNNGC 3 cut(s) 80, 302, 370
BstNI CCWGG 1 cut(s) 639
BstSCI CCNGG 1 cut(s) 637
BstSLI GKGCMC 2 cut(s) 366, 1013
BstV1I GCAGC 5 cut(s) 100, 355, 613, 616, 719
BstV2I GAAGAC 1 cut(s) 786
BstX2I RGATCY 1 cut(s) 152
BstXI CCANNNNNNTGG 3 cut(s) 351, 736, 1021
BstYI RGATCY 1 cut(s) 152
BsuRI GGCC 2 cut(s) 447, 794
BtgI CCRYGG 1 cut(s) 613
BtrI CACGTC 1 cut(s) 385
BtsCI GGATG 2 cut(s) 730, 745
BtsIMutI CAGTG 1 cut(s) 1017
Cac8I GCNNGC 3 cut(s) 366, 634, 705
CfoI GCGC 2 cut(s) 83, 373
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 4 cut(s) 445, 516, 792, 944
Csp6I GTAC 1 cut(s) 750
CviAII CATG 1 cut(s) 837
CviQI GTAC 1 cut(s) 750
DdeI CTNAG 1 cut(s) 1083
DpnI GATC 6 cut(s) 31, 154, 494, 762, 845, 1183
DpnII GATC 6 cut(s) 29, 152, 492, 760, 843, 1181
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 2 cut(s) 350, 1005
Eco24I GRGCYC 1 cut(s) 705
Eco47I GGWCC 2 cut(s) 516, 944
Eco57I CTGAAG 1 cut(s) 646
Eco88I CYCGRG 1 cut(s) 984
EcoRII CCWGG 1 cut(s) 637
EcoT14I CCWWGG 2 cut(s) 350, 1005
EcoT38I GRGCYC 1 cut(s) 705
ErhI CCWWGG 2 cut(s) 350, 1005
FaeI CATG 1 cut(s) 840
FaiI YATR 6 cut(s) 34, 471, 678, 838, 1062, 1151
FaqI GGGAC 1 cut(s) 1065
FatI CATG 1 cut(s) 836
FauI CCCGC 4 cut(s) 343, 373, 434, 659
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 5 cut(s) 114, 369, 627, 630, 708
FokI GGATG 2 cut(s) 737, 752
FriOI GRGCYC 1 cut(s) 705
Fsp4HI GCNGC 5 cut(s) 114, 369, 627, 630, 708
FspBI CTAG 3 cut(s) 588, 851, 1172
FspI TGCGCA 1 cut(s) 372
GlaI GCGC 2 cut(s) 82, 372
GluI GCNGC 5 cut(s) 114, 369, 627, 630, 708
GsaI CCCAGC 2 cut(s) 257, 552
GsuI CTGGAG 1 cut(s) 942
HaeIII GGCC 2 cut(s) 447, 794
HapII CCGG 5 cut(s) 117, 514, 561, 623, 947
HhaI GCGC 2 cut(s) 83, 373
Hin1II CATG 1 cut(s) 840
Hin6I GCGC 2 cut(s) 81, 371
HinP1I GCGC 2 cut(s) 81, 371
HincII GTYRAC 2 cut(s) 124, 1080
HindII GTYRAC 2 cut(s) 124, 1080
HindIII AAGCTT 2 cut(s) 817, 1064
HinfI GANTC 6 cut(s) 20, 49, 158, 377, 432, 557
HpaI GTTAAC 1 cut(s) 1080
HpaII CCGG 5 cut(s) 117, 514, 561, 623, 947
Hpy166II GTNNAC 3 cut(s) 124, 613, 1080
Hpy188I TCNGA 9 cut(s) 157, 163, 193, 431, 575, 857, 864, 884, 974
Hpy188III TCNNGA 5 cut(s) 490, 588, 758, 899, 959
Hpy8I GTNNAC 3 cut(s) 124, 613, 1080
Hpy99I CGWCG 1 cut(s) 150
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 3 cut(s) 397, 877, 1021
HpyCH4IV ACGT 4 cut(s) 132, 384, 525, 609
HpyCH4V TGCA 5 cut(s) 130, 227, 407, 911, 1108
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 302, 370
HpyF3I CTNAG 1 cut(s) 1083
HpySE526I ACGT 4 cut(s) 132, 384, 525, 609
Hsp92II CATG 1 cut(s) 840
HspAI GCGC 2 cut(s) 81, 371
KpnI GGTACC 1 cut(s) 753
KspAI GTTAAC 1 cut(s) 1080
Kzo9I GATC 6 cut(s) 29, 152, 492, 760, 843, 1181
LmnI GCTCC 2 cut(s) 432, 949
Lsp1109I GCAGC 5 cut(s) 100, 355, 613, 616, 719
LweI GCATC 2 cut(s) 283, 715
MaeI CTAG 3 cut(s) 588, 851, 1172
MaeII ACGT 4 cut(s) 132, 384, 525, 609
MaeIII GTNAC 4 cut(s) 605, 656, 871, 1027
MalI GATC 6 cut(s) 31, 154, 494, 762, 845, 1183
MboI GATC 6 cut(s) 29, 152, 492, 760, 843, 1181
MboII GAAGA 6 cut(s) 29, 228, 791, 853, 1036, 1188
MfeI CAATTG 1 cut(s) 75
MflI RGATCY 1 cut(s) 152
MhlI GDGCHC 3 cut(s) 366, 705, 1013
MluCI AATT 5 cut(s) 75, 93, 591, 935, 1097
MlyI GAGTC 3 cut(s) 29, 43, 566
MmeI TCCRAC 2 cut(s) 922, 1115
MnlI CCTC 7 cut(s) 250, 253, 451, 794, 966, 993, 1080
MseI TTAA 4 cut(s) 507, 522, 1079, 1201
MslI CAYNNNNRTG 1 cut(s) 1127
MspA1I CMGCKG 2 cut(s) 368, 666
MspI CCGG 5 cut(s) 117, 514, 561, 623, 947
MspR9I CCNGG 1 cut(s) 639
MunI CAATTG 1 cut(s) 75
MvaI CCWGG 1 cut(s) 639
MwoI GCNNNNNNNGC 3 cut(s) 80, 302, 370
NdeII GATC 6 cut(s) 29, 152, 492, 760, 843, 1181
NlaIII CATG 1 cut(s) 840
NlaIV GGNNCC 5 cut(s) 154, 446, 751, 1012, 1091
NmuCI GTSAC 3 cut(s) 656, 871, 1027
NsbI TGCGCA 1 cut(s) 372
PaeR7I CTCGAG 1 cut(s) 984
PcsI WCGNNNNNNNCGW 3 cut(s) 129, 381, 473
PfeI GAWTC 3 cut(s) 158, 377, 432
PflFI GACNNNGTC 1 cut(s) 1056
PflMI CCANNNNNTGG 1 cut(s) 1155
PkrI GCNGC 5 cut(s) 115, 370, 628, 631, 709
PleI GAGTC 3 cut(s) 28, 43, 565
PpsI GAGTC 3 cut(s) 28, 43, 565
Ppu21I YACGTR 1 cut(s) 133
Psp6I CCWGG 1 cut(s) 637
PspFI CCCAGC 2 cut(s) 253, 548
PspGI CCWGG 1 cut(s) 637
PspN4I GGNNCC 5 cut(s) 154, 446, 751, 1012, 1091
PspPI GGNCC 4 cut(s) 445, 516, 792, 944
PspXI VCTCGAGB 1 cut(s) 984
PsrI GAACNNNNNNTAC 2 cut(s) 465, 497
PsuI RGATCY 1 cut(s) 152
PsyI GACNNNGTC 1 cut(s) 1056
RsaI GTAC 1 cut(s) 751
RsaNI GTAC 1 cut(s) 750
RseI CAYNNNNRTG 1 cut(s) 1127
SalI GTCGAC 1 cut(s) 122
SaqAI TTAA 4 cut(s) 507, 522, 1079, 1201
SatI GCNGC 5 cut(s) 114, 369, 627, 630, 708
Sau3AI GATC 6 cut(s) 29, 152, 492, 760, 843, 1181
Sau96I GGNCC 4 cut(s) 445, 516, 792, 944
SchI GAGTC 3 cut(s) 29, 43, 566
ScrFI CCNGG 1 cut(s) 639
SduI GDGCHC 3 cut(s) 366, 705, 1013
SfaNI GCATC 2 cut(s) 283, 715
Sfr274I CTCGAG 1 cut(s) 984
SinI GGWCC 2 cut(s) 516, 944
SlaI CTCGAG 1 cut(s) 984
SmiMI CAYNNNNRTG 1 cut(s) 1127
SmlI CTYRAG 2 cut(s) 541, 984
SmoI CTYRAG 2 cut(s) 541, 984
SpeI ACTAGT 1 cut(s) 850
Sse9I AATT 5 cut(s) 75, 93, 591, 935, 1097
SsiI CCGC 4 cut(s) 336, 366, 441, 666
SspMI CTAG 3 cut(s) 588, 851, 1172
StyD4I CCNGG 1 cut(s) 637
StyI CCWWGG 2 cut(s) 350, 1005
TaaI ACNGT 3 cut(s) 397, 877, 1021
TaiI ACGT 4 cut(s) 135, 387, 528, 612
TaqI TCGA 8 cut(s) 123, 145, 329, 456, 476, 555, 985, 1180
TasI AATT 5 cut(s) 75, 93, 591, 935, 1097
TfiI GAWTC 3 cut(s) 158, 377, 432
Tru1I TTAA 4 cut(s) 507, 522, 1079, 1201
Tru9I TTAA 4 cut(s) 507, 522, 1079, 1201
TscAI CASTG 1 cut(s) 1024
TseFI GTSAC 3 cut(s) 656, 871, 1027
TseI GCWGC 5 cut(s) 113, 368, 626, 629, 707
Tsp45I GTSAC 3 cut(s) 656, 871, 1027
TspDTI ATGAA 3 cut(s) 105, 958, 1024
TspRI CASTG 1 cut(s) 1024
Tth111I GACNNNGTC 1 cut(s) 1056
Van91I CCANNNNNTGG 1 cut(s) 1155
VpaK11BI GGWCC 2 cut(s) 516, 944
XapI RAATTY 1 cut(s) 1097
XbaI TCTAGA 1 cut(s) 587
XhoI CTCGAG 1 cut(s) 984
XmiI GTMKAC 1 cut(s) 123
XspI CTAG 3 cut(s) 588, 851, 1172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.