RLG00000034434

Non-specific serine threonine protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0024724
Physical Location & Seq
Reverse (-)
42711578 .. 42712908
1331 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034434

Sequence Viewer

Length: 588 bp
ATGATCAAGGTGAAGACGCATGCTAAGGGGAGAGAAAATAGGAGTCACTGTGCAACAGGTGGTTTTCAAGCTCAAACAAAAGATTCAGAACAAAACAGCGCTGTTCGAAAAGAATGCGTTAGGTTGATGGGTACATTGGTGAGGTATCATGAGGGCTATGTTAGAATGAATCTTGCTCTTCTATTCGTCTCCGCCCTTCGCTTCTGCCACGAAAACGGCGTCACTCACCGCAACGTCAAGCCTCAGAACCTCCTCCTCGAAGGCACCGGAAACTTGAAGGTCTCCGAATTCAGACCCTCCGACCTGCCGGAGCAGCTCAAGGACGCCCTCCTCCACACGGTTTGCGGGGTGCCGGCATACACGAGGCTGGAGGTTCTGTATCGGGTAGGGTGCGACAGATCCAAGGCTGACGCGTGGTCTTGCGGAGTTATCCTCTTCGTTTTCCTCGCCGGACACTTGTGCATGATCAGGCTCTCTCTCTCTCCCCCATCGCCACCTGGCTCTGCCACCATCGCCGTTGTCGTGCTCTCCGCAGCCAGGCTCGTCGCAGAGGAGAAAGATGATTTGGTGCCCAAAGCTATTCTCTAG

Protein Analysis

196

Amino Acids

21.45

Weight (kDa)

9.15

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 58 - 153 1.6e-17 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024724)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0339601
rosa_laevigata RLG00000034434
rosa_multiflora Rmu_ssc0000009.1_g000027

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 312
AccB1I GGYRCC 3 cut(s) 263, 349, 568
AccII CGCG 1 cut(s) 413
AciI CCGC 5 cut(s) 192, 229, 345, 423, 531
AclWI GGATC 1 cut(s) 393
AcsI RAATTY 1 cut(s) 287
AcyI GRCGYC 2 cut(s) 219, 324
AfaI GTAC 1 cut(s) 133
AfeI AGCGCT 1 cut(s) 100
AfiI CCNNNNNNNGG 2 cut(s) 337, 537
AflIII ACRYGT 1 cut(s) 411
AgsI TTSAA 2 cut(s) 68, 277
AhdI GACNNNNNGTC 1 cut(s) 415
AjnI CCWGG 2 cut(s) 496, 536
AjuI GAANNNNNNNTTGG 2 cut(s) 548, 580
AluBI AGCT 3 cut(s) 71, 316, 578
AluI AGCT 3 cut(s) 71, 316, 578
Alw21I GWGCWC 1 cut(s) 528
Alw26I GTCTC 2 cut(s) 193, 286
AlwI GGATC 1 cut(s) 393
Aor51HI AGCGCT 1 cut(s) 100
ApeKI GCWGC 2 cut(s) 313, 533
ApoI RAATTY 1 cut(s) 287
ArsI GACNNNNNNTTYG 2 cut(s) 204, 236
AspLEI GCGC 1 cut(s) 101
AsuHPI GGTGA 3 cut(s) 22, 151, 218
AsuII TTCGAA 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 573
BaeI ACNNNNGTAYC 2 cut(s) 123, 156
BanI GGYRCC 3 cut(s) 263, 349, 568
BauI CACGAG 1 cut(s) 361
BbsI GAAGAC 1 cut(s) 20
Bbv12I GWGCWC 1 cut(s) 528
BbvI GCAGC 2 cut(s) 325, 545
BccI CCATC 3 cut(s) 121, 496, 518
BceAI ACGGC 2 cut(s) 232, 500
BcgI CGANNNNNNTGC 2 cut(s) 96, 130
BciT130I CCWGG 2 cut(s) 498, 538
BclI TGATCA 2 cut(s) 3, 465
BcoDI GTCTC 2 cut(s) 193, 286
BfaI CTAG 1 cut(s) 586
BfoI RGCGCY 1 cut(s) 102
BfuAI ACCTGC 1 cut(s) 312
BisI GCNGC 2 cut(s) 314, 534
BlsI GCNGC 2 cut(s) 315, 535
Bme1390I CCNGG 2 cut(s) 498, 538
BmeRI GACNNNNNGTC 1 cut(s) 415
BmiI GGNNCC 3 cut(s) 265, 351, 570
BmrFI CCNGG 2 cut(s) 498, 538
BpiI GAAGAC 1 cut(s) 20
BplI GAGNNNNNCTC 2 cut(s) 417, 449
BpmI CTGGAG 1 cut(s) 389
Bpu10I CCTNAGC 1 cut(s) 24
Bpu14I TTCGAA 1 cut(s) 106
BpuEI CTTGAG 1 cut(s) 302
BsaHI GRCGYC 2 cut(s) 219, 324
BsaI GGTCTC 1 cut(s) 286
BsaJI CCNNGG 1 cut(s) 402
BsaWI WCCGGW 1 cut(s) 266
BsaXI ACNNNNNCTCC 4 cut(s) 34, 64, 362, 392
Bsc4I CCNNNNNNNGG 2 cut(s) 337, 537
Bse118I RCCGGY 1 cut(s) 352
BseBI CCWGG 2 cut(s) 498, 538
BseDI CCNNGG 1 cut(s) 402
BseLI CCNNNNNNNGG 2 cut(s) 337, 537
BseMII CTCAG 1 cut(s) 257
BseRI GAGGAG 4 cut(s) 242, 245, 320, 566
BseSI GKGCMC 1 cut(s) 573
BseXI GCAGC 2 cut(s) 325, 545
Bsh1236I CGCG 1 cut(s) 413
BshNI GGYRCC 3 cut(s) 263, 349, 568
BsiHKAI GWGCWC 1 cut(s) 528
BsiSI CCGG 4 cut(s) 267, 308, 353, 450
BslI CCNNNNNNNGG 2 cut(s) 337, 537
BsmAI GTCTC 2 cut(s) 193, 286
BsmBI CGTCTC 1 cut(s) 193
BsmI GAATGC 1 cut(s) 119
Bso31I GGTCTC 1 cut(s) 286
Bsp119I TTCGAA 1 cut(s) 106
Bsp1286I GDGCHC 2 cut(s) 528, 573
Bsp143I GATC 3 cut(s) 3, 398, 465
BspACI CCGC 5 cut(s) 192, 229, 345, 423, 531
BspCNI CTCAG 1 cut(s) 256
BspFNI CGCG 1 cut(s) 413
BspHI TCATGA 1 cut(s) 148
BspLI GGNNCC 3 cut(s) 265, 351, 570
BspMI ACCTGC 1 cut(s) 312
BspPI GGATC 1 cut(s) 393
BspQI GCTCTTC 1 cut(s) 183
BspT104I TTCGAA 1 cut(s) 106
BspT107I GGYRCC 3 cut(s) 263, 349, 568
BspTNI GGTCTC 1 cut(s) 286
BsrFI RCCGGY 1 cut(s) 352
BssAI RCCGGY 1 cut(s) 352
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 3 cut(s) 3, 398, 465
BssNI GRCGYC 2 cut(s) 219, 324
BssSI CACGAG 1 cut(s) 361
BssT1I CCWWGG 1 cut(s) 402
Bst2BI CACGAG 1 cut(s) 361
Bst2UI CCWGG 2 cut(s) 498, 538
Bst4CI ACNGT 2 cut(s) 50, 340
Bst6I CTCTTC 2 cut(s) 183, 440
BstACI GRCGYC 2 cut(s) 219, 324
BstBI TTCGAA 1 cut(s) 106
BstC8I GCNNGC 2 cut(s) 21, 354
BstDEI CTNAG 2 cut(s) 24, 243
BstFNI CGCG 1 cut(s) 413
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 3 cut(s) 6, 401, 468
BstMAI GTCTC 2 cut(s) 193, 286
BstMBI GATC 3 cut(s) 3, 398, 465
BstMWI GCNNNNNNNGC 2 cut(s) 313, 512
BstNI CCWGG 2 cut(s) 498, 538
BstNSI RCATGY 1 cut(s) 23
BstSCI CCNGG 2 cut(s) 496, 536
BstSLI GKGCMC 1 cut(s) 573
BstUI CGCG 1 cut(s) 413
BstV1I GCAGC 2 cut(s) 325, 545
BstV2I GAAGAC 1 cut(s) 20
BstX2I RGATCY 1 cut(s) 398
BstYI RGATCY 1 cut(s) 398
BtgZI GCGATG 2 cut(s) 474, 496
BtsIMutI CAGTG 1 cut(s) 46
BveI ACCTGC 1 cut(s) 312
Cac8I GCNNGC 2 cut(s) 21, 354
CciI TCATGA 1 cut(s) 148
CfoI GCGC 1 cut(s) 101
Cfr10I RCCGGY 1 cut(s) 352
CseI GACGC 4 cut(s) 25, 208, 332, 419
Csp6I GTAC 1 cut(s) 132
CspCI CAANNNNNGTGG 2 cut(s) 323, 358
CviAII CATG 3 cut(s) 20, 149, 463
CviQI GTAC 1 cut(s) 132
DdeI CTNAG 2 cut(s) 24, 243
DpnI GATC 3 cut(s) 5, 400, 467
DpnII GATC 3 cut(s) 3, 398, 465
DriI GACNNNNNGTC 1 cut(s) 415
Eam1104I CTCTTC 2 cut(s) 183, 440
Eam1105I GACNNNNNGTC 1 cut(s) 415
EarI CTCTTC 2 cut(s) 183, 440
EciI GGCGGA 1 cut(s) 181
Eco130I CCWWGG 1 cut(s) 402
Eco31I GGTCTC 1 cut(s) 286
Eco47III AGCGCT 1 cut(s) 100
EcoRI GAATTC 1 cut(s) 287
EcoRII CCWGG 2 cut(s) 496, 536
EcoT14I CCWWGG 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 402
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 3 cut(s) 23, 152, 466
FaiI YATR 5 cut(s) 21, 150, 159, 358, 464
FatI CATG 3 cut(s) 19, 148, 462
FauI CCCGC 1 cut(s) 338
FbaI TGATCA 2 cut(s) 3, 465
Fnu4HI GCNGC 2 cut(s) 314, 534
Fsp4HI GCNGC 2 cut(s) 314, 534
FspBI CTAG 1 cut(s) 586
GlaI GCGC 1 cut(s) 100
GluI GCNGC 2 cut(s) 314, 534
GsuI CTGGAG 1 cut(s) 389
HaeII RGCGCY 1 cut(s) 102
HapII CCGG 4 cut(s) 267, 308, 353, 450
HgaI GACGC 4 cut(s) 25, 208, 332, 419
HhaI GCGC 1 cut(s) 101
Hin1I GRCGYC 2 cut(s) 219, 324
Hin1II CATG 3 cut(s) 23, 152, 466
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HinfI GANTC 3 cut(s) 43, 83, 169
HpaII CCGG 4 cut(s) 267, 308, 353, 450
HphI GGTGA 3 cut(s) 22, 151, 218
Hpy188I TCNGA 5 cut(s) 88, 246, 286, 293, 301
Hpy188III TCNNGA 1 cut(s) 149
Hpy99I CGWCG 1 cut(s) 548
HpyAV CCTTC 3 cut(s) 206, 254, 271
HpyCH4III ACNGT 2 cut(s) 50, 340
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 2 cut(s) 53, 462
HpyF10VI GCNNNNNNNGC 2 cut(s) 313, 512
HpyF3I CTNAG 2 cut(s) 24, 243
HpySE526I ACGT 1 cut(s) 234
Hsp92I GRCGYC 2 cut(s) 219, 324
Hsp92II CATG 3 cut(s) 23, 152, 466
HspAI GCGC 1 cut(s) 99
KroI GCCGGC 1 cut(s) 352
KroNI GCCGGC 1 cut(s) 354
Ksp22I TGATCA 2 cut(s) 3, 465
Kzo9I GATC 3 cut(s) 3, 398, 465
LguI GCTCTTC 1 cut(s) 183
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 2 cut(s) 325, 545
MaeI CTAG 1 cut(s) 586
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 2 cut(s) 44, 220
MalI GATC 3 cut(s) 5, 400, 467
MboI GATC 3 cut(s) 3, 398, 465
MboII GAAGA 3 cut(s) 25, 170, 427
MflI RGATCY 1 cut(s) 398
MhlI GDGCHC 2 cut(s) 528, 573
MluCI AATT 1 cut(s) 287
MluI ACGCGT 1 cut(s) 411
MlyI GAGTC 1 cut(s) 52
MmeI TCCRAC 1 cut(s) 324
MroNI GCCGGC 1 cut(s) 352
MspI CCGG 4 cut(s) 267, 308, 353, 450
MspR9I CCNGG 2 cut(s) 498, 538
Mva1269I GAATGC 1 cut(s) 119
MvaI CCWGG 2 cut(s) 498, 538
MvnI CGCG 1 cut(s) 413
MwoI GCNNNNNNNGC 2 cut(s) 313, 512
NaeI GCCGGC 1 cut(s) 354
NdeII GATC 3 cut(s) 3, 398, 465
NgoMIV GCCGGC 1 cut(s) 352
NlaIII CATG 3 cut(s) 23, 152, 466
NlaIV GGNNCC 3 cut(s) 265, 351, 570
NmuCI GTSAC 2 cut(s) 44, 220
NspI RCATGY 1 cut(s) 23
NspV TTCGAA 1 cut(s) 106
PaeI GCATGC 1 cut(s) 23
PagI TCATGA 1 cut(s) 148
PciSI GCTCTTC 1 cut(s) 183
PcsI WCGNNNNNNNCGW 2 cut(s) 216, 519
PctI GAATGC 1 cut(s) 119
PdiI GCCGGC 1 cut(s) 354
PfeI GAWTC 2 cut(s) 83, 169
PkrI GCNGC 2 cut(s) 315, 535
PleI GAGTC 1 cut(s) 51
PpsI GAGTC 1 cut(s) 51
Psp6I CCWGG 2 cut(s) 496, 536
PspGI CCWGG 2 cut(s) 496, 536
PspN4I GGNNCC 3 cut(s) 265, 351, 570
PsuI RGATCY 1 cut(s) 398
RsaI GTAC 1 cut(s) 133
RsaNI GTAC 1 cut(s) 132
SapI GCTCTTC 1 cut(s) 183
SatI GCNGC 2 cut(s) 314, 534
Sau3AI GATC 3 cut(s) 3, 398, 465
SchI GAGTC 1 cut(s) 52
ScrFI CCNGG 2 cut(s) 498, 538
SduI GDGCHC 2 cut(s) 528, 573
SfuI TTCGAA 1 cut(s) 106
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
SphI GCATGC 1 cut(s) 23
Sse9I AATT 1 cut(s) 287
SsiI CCGC 5 cut(s) 192, 229, 345, 423, 531
SspMI CTAG 1 cut(s) 586
StyD4I CCNGG 2 cut(s) 496, 536
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 2 cut(s) 50, 340
TaiI ACGT 1 cut(s) 237
TaqI TCGA 2 cut(s) 106, 258
TasI AATT 1 cut(s) 287
TfiI GAWTC 2 cut(s) 83, 169
TscAI CASTG 1 cut(s) 53
TseFI GTSAC 2 cut(s) 44, 220
TseI GCWGC 2 cut(s) 313, 533
Tsp45I GTSAC 2 cut(s) 44, 220
TspDTI ATGAA 1 cut(s) 182
TspRI CASTG 1 cut(s) 53
XapI RAATTY 1 cut(s) 287
XceI RCATGY 1 cut(s) 23
XspI CTAG 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.