RLG00000035393

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0020546
Physical Location & Seq
Reverse (-)
66236303 .. 66240231
3929 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035393

Sequence Viewer

Length: 603 bp
ATGGCGGCGGAGGAGGGCAGCGTCTGTAAGCGAGCTCACACCGAGTTCGACGACGGCGAAATCATTTCAGAGATTCTGGTGTTACCAAGAACTCCACTGCCCGTTTTTAATCCACGTGGTTGTTGCAATGGTGGGTTTGGTTACGATTCTGCTACTGATGATTACAAGCTAATAGTGGGGACTAAAGAGATGGGTGGAGAAAATTCCATTCAGGTTTTCACTCTGAAAACGAGTACATGGAAGGTACCACCAACAGAGTTCTCATCGAAGTACTTCGATTCGCTTGGAGCTTCAATCAAACTCGCTCCGGTGGATATTCAATTCTATTTTAAAACAACTCGAGGTAAAAGCAATCACCGTTACGGTGAGGCCGCTTTTAACTGTCAACTGTCTCTTGTAAGTGGTTCTCCAGACTTCCCGCCACATATATCTGTAATTGTCAGCGCTGAACCTGGTCCGGTAGAAGATGCCTACTGGAAAAAACGTGAAGCCGATGCTCAGAATGTAGCCGAGCAATCTGTTGAACCACATCCGGAGCAAGTGACCGAGGAATTGAATACTAATGTTGGCCAGCTTGTATCAGAGCAAGATAAGCATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1 FBA_3

Protein Analysis

201

Amino Acids

22.02

Weight (kDa)

4.96

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pec_lyase_N PF04431 156 - 195 1.5e-08 Pectate lyase, N terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020546)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0060721
rosa_laevigata RLG00000035393
rosa_roxburghii Rroxscaffold_1G00020000
rosa_rugosa Rorug05G0333200
rosa_samantha Rh5CG432900 Rh5DG423200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 244
AccB1I GGYRCC 1 cut(s) 244
AccIII TCCGGA 1 cut(s) 532
AciI CCGC 4 cut(s) 5, 8, 372, 419
AcoI YGGCCR 1 cut(s) 568
AcsI RAATTY 1 cut(s) 202
AcvI CACGTG 1 cut(s) 116
AfaI GTAC 3 cut(s) 235, 246, 272
AfeI AGCGCT 1 cut(s) 445
AgsI TTSAA 4 cut(s) 294, 320, 524, 556
AjnI CCWGG 1 cut(s) 451
AluBI AGCT 4 cut(s) 35, 169, 290, 574
AluI AGCT 4 cut(s) 35, 169, 290, 574
Alw21I GWGCWC 1 cut(s) 37
Alw26I GTCTC 1 cut(s) 396
AlwNI CAGNNNCTG 1 cut(s) 24
Ama87I CYCGRG 1 cut(s) 339
Aor13HI TCCGGA 1 cut(s) 532
Aor51HI AGCGCT 1 cut(s) 445
AoxI GGCC 2 cut(s) 369, 568
ApeKI GCWGC 1 cut(s) 18
ApoI RAATTY 1 cut(s) 202
Asp700I GAANNNNTTC 1 cut(s) 272
Asp718I GGTACC 1 cut(s) 244
AspLEI GCGC 1 cut(s) 446
AspS9I GGNCC 1 cut(s) 455
AsuHPI GGTGA 2 cut(s) 347, 377
AvaI CYCGRG 1 cut(s) 339
AvaII GGWCC 1 cut(s) 455
BalI TGGCCA 1 cut(s) 570
BanI GGYRCC 1 cut(s) 244
BanII GRGCYC 1 cut(s) 37
BbrPI CACGTG 1 cut(s) 116
Bbv12I GWGCWC 1 cut(s) 37
BbvI GCAGC 1 cut(s) 30
BccI CCATC 1 cut(s) 184
BceAI ACGGC 1 cut(s) 70
BciT130I CCWGG 1 cut(s) 453
BcoDI GTCTC 1 cut(s) 396
BfoI RGCGCY 1 cut(s) 447
BisI GCNGC 3 cut(s) 6, 19, 372
BlsI GCNGC 3 cut(s) 7, 20, 373
BmcAI AGTACT 1 cut(s) 272
Bme1390I CCNGG 1 cut(s) 453
Bme18I GGWCC 1 cut(s) 455
BmeT110I CYCGRG 1 cut(s) 339
BmgT120I GGNCC 1 cut(s) 455
BmiI GGNNCC 1 cut(s) 246
BmrFI CCNGG 1 cut(s) 453
BmsI GCATC 2 cut(s) 457, 484
BpmI CTGGAG 1 cut(s) 393
BsaAI YACGTR 1 cut(s) 116
BsaBI GATNNNNATC 1 cut(s) 594
BsaJI CCNNGG 1 cut(s) 546
BsaWI WCCGGW 3 cut(s) 307, 457, 532
Bse1I ACTGG 1 cut(s) 479
Bse3DI GCAATG 1 cut(s) 133
Bse8I GATNNNNATC 1 cut(s) 594
BseAI TCCGGA 1 cut(s) 532
BseBI CCWGG 1 cut(s) 453
BseDI CCNNGG 1 cut(s) 546
BseGI GGATG 1 cut(s) 529
BseJI GATNNNNATC 1 cut(s) 594
BseMI GCAATG 1 cut(s) 133
BseMII CTCAG 1 cut(s) 512
BseNI ACTGG 1 cut(s) 479
BseRI GAGGAG 1 cut(s) 26
BseXI GCAGC 1 cut(s) 30
BshFI GGCC 2 cut(s) 371, 570
BshNI GGYRCC 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 37
BsiHKCI CYCGRG 1 cut(s) 339
BsiSI CCGG 3 cut(s) 308, 458, 533
BslFI GGGAC 1 cut(s) 193
BsmAI GTCTC 1 cut(s) 396
BsmFI GGGAC 1 cut(s) 193
BsnI GGCC 2 cut(s) 371, 570
BsoBI CYCGRG 1 cut(s) 339
Bsp1286I GDGCHC 1 cut(s) 37
Bsp13I TCCGGA 1 cut(s) 532
BspACI CCGC 4 cut(s) 5, 8, 372, 419
BspANI GGCC 2 cut(s) 371, 570
BspCNI CTCAG 1 cut(s) 511
BspEI TCCGGA 1 cut(s) 532
BspLI GGNNCC 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 244
BsrDI GCAATG 1 cut(s) 133
BsrI ACTGG 1 cut(s) 479
BssECI CCNNGG 1 cut(s) 546
Bst2UI CCWGG 1 cut(s) 453
Bst4CI ACNGT 4 cut(s) 359, 365, 383, 390
BstBAI YACGTR 1 cut(s) 116
BstC8I GCNNGC 2 cut(s) 33, 572
BstDEI CTNAG 1 cut(s) 498
BstF5I GGATG 1 cut(s) 529
BstH2I RGCGCY 1 cut(s) 447
BstHHI GCGC 1 cut(s) 446
BstMAI GTCTC 1 cut(s) 396
BstMWI GCNNNNNNNGC 1 cut(s) 592
BstNI CCWGG 1 cut(s) 453
BstSCI CCNGG 1 cut(s) 451
BstV1I GCAGC 1 cut(s) 30
BsuRI GGCC 2 cut(s) 371, 570
BtsCI GGATG 1 cut(s) 529
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 1 cut(s) 95
Cac8I GCNNGC 2 cut(s) 33, 572
CaiI CAGNNNCTG 1 cut(s) 24
CfoI GCGC 1 cut(s) 446
Cfr13I GGNCC 1 cut(s) 455
CseI GACGC 1 cut(s) 10
CsiI ACCWGGT 1 cut(s) 451
Csp6I GTAC 3 cut(s) 234, 245, 271
CviAII CATG 1 cut(s) 237
CviJI RGCY 8 cut(s) 35, 169, 290, 371, 491, 509, 570, 574
CviKI_1 RGCY 8 cut(s) 35, 169, 290, 371, 491, 509, 570, 574
CviQI GTAC 3 cut(s) 234, 245, 271
DdeI CTNAG 1 cut(s) 498
DraI TTTAAA 1 cut(s) 331
EaeI YGGCCR 1 cut(s) 568
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 35
Eco24I GRGCYC 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 455
Eco47III AGCGCT 1 cut(s) 445
Eco53kI GAGCTC 1 cut(s) 35
Eco72I CACGTG 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 339
EcoICRI GAGCTC 1 cut(s) 35
EcoRII CCWGG 1 cut(s) 451
EcoT38I GRGCYC 1 cut(s) 37
FaeI CATG 1 cut(s) 240
FaiI YATR 3 cut(s) 238, 426, 428
FaqI GGGAC 1 cut(s) 193
FatI CATG 1 cut(s) 236
FauI CCCGC 1 cut(s) 426
Fnu4HI GCNGC 3 cut(s) 6, 19, 372
FokI GGATG 1 cut(s) 516
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 3 cut(s) 6, 19, 372
GlaI GCGC 1 cut(s) 445
GluI GCNGC 3 cut(s) 6, 19, 372
GsuI CTGGAG 1 cut(s) 393
HaeII RGCGCY 1 cut(s) 447
HaeIII GGCC 2 cut(s) 371, 570
HapII CCGG 3 cut(s) 308, 458, 533
HgaI GACGC 1 cut(s) 10
HhaI GCGC 1 cut(s) 446
Hin1II CATG 1 cut(s) 240
Hin6I GCGC 1 cut(s) 444
HinP1I GCGC 1 cut(s) 444
HincII GTYRAC 1 cut(s) 386
HindII GTYRAC 1 cut(s) 386
HinfI GANTC 3 cut(s) 73, 146, 278
HpaII CCGG 3 cut(s) 308, 458, 533
HphI GGTGA 2 cut(s) 347, 377
Hpy166II GTNNAC 1 cut(s) 386
Hpy188I TCNGA 4 cut(s) 70, 225, 501, 583
Hpy188III TCNNGA 2 cut(s) 410, 533
Hpy8I GTNNAC 1 cut(s) 386
Hpy99I CGWCG 2 cut(s) 53, 56
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 4 cut(s) 359, 365, 383, 390
HpyCH4IV ACGT 2 cut(s) 115, 484
HpyCH4V TGCA 1 cut(s) 126
HpyF10VI GCNNNNNNNGC 1 cut(s) 592
HpyF3I CTNAG 1 cut(s) 498
HpySE526I ACGT 2 cut(s) 115, 484
Hsp92II CATG 1 cut(s) 240
HspAI GCGC 1 cut(s) 444
Kpn2I TCCGGA 1 cut(s) 532
KpnI GGTACC 1 cut(s) 248
LmnI GCTCC 3 cut(s) 287, 310, 535
Lsp1109I GCAGC 1 cut(s) 30
LweI GCATC 2 cut(s) 457, 484
MabI ACCWGGT 1 cut(s) 451
MaeII ACGT 2 cut(s) 115, 484
MaeIII GTNAC 4 cut(s) 81, 140, 359, 541
MboII GAAGA 1 cut(s) 476
MhlI GDGCHC 1 cut(s) 37
MlsI TGGCCA 1 cut(s) 570
MluCI AATT 4 cut(s) 202, 320, 435, 551
MluNI TGGCCA 1 cut(s) 570
MnlI CCTC 5 cut(s) 4, 7, 335, 361, 541
Mox20I TGGCCA 1 cut(s) 570
MroI TCCGGA 1 cut(s) 532
MroXI GAANNNNTTC 1 cut(s) 272
MscI TGGCCA 1 cut(s) 570
MseI TTAA 3 cut(s) 108, 330, 378
Msp20I TGGCCA 1 cut(s) 570
MspI CCGG 3 cut(s) 308, 458, 533
MspR9I CCNGG 1 cut(s) 453
MvaI CCWGG 1 cut(s) 453
MwoI GCNNNNNNNGC 1 cut(s) 592
NlaIII CATG 1 cut(s) 240
NlaIV GGNNCC 1 cut(s) 246
NmeAIII GCCGAG 1 cut(s) 535
NmuCI GTSAC 1 cut(s) 541
PaeR7I CTCGAG 1 cut(s) 339
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PdmI GAANNNNTTC 1 cut(s) 272
PfeI GAWTC 3 cut(s) 73, 146, 278
PkrI GCNGC 3 cut(s) 7, 20, 373
PmaCI CACGTG 1 cut(s) 116
PmlI CACGTG 1 cut(s) 116
Ppu21I YACGTR 1 cut(s) 116
Psp124BI GAGCTC 1 cut(s) 37
Psp6I CCWGG 1 cut(s) 451
PspCI CACGTG 1 cut(s) 116
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 1 cut(s) 246
PspPI GGNCC 1 cut(s) 455
PspXI VCTCGAGB 1 cut(s) 339
PstNI CAGNNNCTG 1 cut(s) 24
RsaI GTAC 3 cut(s) 235, 246, 272
RsaNI GTAC 3 cut(s) 234, 245, 271
SacI GAGCTC 1 cut(s) 37
SaqAI TTAA 3 cut(s) 108, 330, 378
SatI GCNGC 3 cut(s) 6, 19, 372
Sau96I GGNCC 1 cut(s) 455
ScaI AGTACT 1 cut(s) 272
ScrFI CCNGG 1 cut(s) 453
SduI GDGCHC 1 cut(s) 37
SexAI ACCWGGT 1 cut(s) 451
SfaNI GCATC 2 cut(s) 457, 484
Sfr274I CTCGAG 1 cut(s) 339
SinI GGWCC 1 cut(s) 455
SlaI CTCGAG 1 cut(s) 339
SmlI CTYRAG 1 cut(s) 339
SmoI CTYRAG 1 cut(s) 339
Sse9I AATT 4 cut(s) 202, 320, 435, 551
SsiI CCGC 4 cut(s) 5, 8, 372, 419
SstI GAGCTC 1 cut(s) 37
StyD4I CCNGG 1 cut(s) 451
TaaI ACNGT 4 cut(s) 359, 365, 383, 390
TaiI ACGT 2 cut(s) 118, 487
TaqI TCGA 4 cut(s) 48, 266, 276, 340
TaqII GACCGA 1 cut(s) 560
TasI AATT 4 cut(s) 202, 320, 435, 551
TatI WGTACW 2 cut(s) 233, 270
TauI GCSGC 2 cut(s) 8, 374
TfiI GAWTC 3 cut(s) 73, 146, 278
Tru1I TTAA 3 cut(s) 108, 330, 378
Tru9I TTAA 3 cut(s) 108, 330, 378
TscAI CASTG 1 cut(s) 102
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 1 cut(s) 18
Tsp45I GTSAC 1 cut(s) 541
TspRI CASTG 1 cut(s) 102
VpaK11BI GGWCC 1 cut(s) 455
XapI RAATTY 1 cut(s) 202
XhoI CTCGAG 1 cut(s) 339
XmnI GAANNNNTTC 1 cut(s) 272
ZrmI AGTACT 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.