Rorug05G0333200

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0020546
Physical Location & Seq
Reverse (-)
40282398 .. 40286987
4590 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0333200.1

Sequence Viewer

Length: 1059 bp
ATGGGTTTGACTTATACTTTTAACACTTATACTCTTTCAACTCGTTCCCTCTCCATTCCATCGAGCAAAACACCCAGTCCGCCTCCAGCTGAAAATCACTCTCTCTCACTCTCATCCCTCAGTCCCGCTCTCAGCCCAGCCCTCAGCCCGCTCTCAGACCACGAACCCGTCTCTCTCGATCAGCCCCGCCCTCAGCCCTCCCTCAGTCCCACTCTCAGTCCCGCCCTCAGCCCGCTCTCAGACCACGAACCGTCTCTACTAAGCAGTTTGAAGGCAGATCCTCTCAAACTTAACCTCCATCAACAACAGCTTCATGGTCTGGAACCCGCCGGAGTTCGTCAGGGATTTGGGGTAAGAAAAAGAAGAGGAGAGATTGAAGATTTTGGTCGACTTTTGGAGGAAGGTTTGGGTTTGTCTTCGAAGAGGTTGCTGGGTTTTCGAAGAGTTTTCTTGTCTGAAGATCAGCTCTTGTCTGTCACCAGTTTGGATGCCGTGGTGTTTATGCGCATTTTCATCTTCAGTTTGAAGGTGTTTGGGTTTGGTGGGATTATTGGGGTTTTTGTTCTTCTGCCAATAAATTTTCTGGGGACCCAGCTGGAGGTGGATTTTTCGGATTTGCCCAACAAGTCTTTGGACTCGTTTAGTATTTCGAATGTGGATGATGGCTCAAACTGGTTATGGGTTCATTTTTGTGCAGCATATATTTTCAGTGCAGTCGTCTGCTATCTTCTTTACAATGAGTACAGTTATATCTCATCAAGAAGAATTGCTCACTTCTATTCATCCAAACCTCAGCCTAATCAGTTCACAGTATTGGTTCGTGCAGCTGCAGTGAAACCGTTGAAAAATTCTTTACAGAATATTACTCATATACTTATCTTTCACACTCGTTGGTTCGTCGAACTAGCAAACTCCAACGTCTCACTAGGCCATGAGTTCAAAAACGAGGGTTTTGGTCCTGGCACAGTTATTTTATGTGATCAGGTTCTTACTGAGGGTAAACAAAAAGATGTACTTAAAGCTGCCGGTGCATTGTTTTCACATATTTGTGAGGTTTAG

Protein Analysis

352

Amino Acids

38.8

Weight (kDa)

6.83

Isoelectric Point (pI)

51.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 151 - 247 1.1e-24 Late exocytosis, associated with Golgi transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020546)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0060721
rosa_laevigata RLG00000035393
rosa_roxburghii Rroxscaffold_1G00020000
rosa_rugosa Rorug05G0333200
rosa_samantha Rh5CG432900 Rh5DG423200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 506
AccBSI CCGCTC 3 cut(s) 128, 151, 235
AccI GTMKAC 1 cut(s) 388
AciI CCGC 7 cut(s) 80, 126, 149, 187, 222, 233, 327
AclWI GGATC 1 cut(s) 272
AcsI RAATTY 2 cut(s) 577, 847
AcuI CTGAAG 2 cut(s) 477, 502
AfaI GTAC 2 cut(s) 743, 1014
AfiI CCNNNNNNNGG 1 cut(s) 598
AgsI TTSAA 6 cut(s) 39, 271, 377, 526, 844, 940
AjnI CCWGG 1 cut(s) 958
AluBI AGCT 6 cut(s) 89, 310, 466, 595, 827, 1022
AluI AGCT 6 cut(s) 89, 310, 466, 595, 827, 1022
Alw26I GTCTC 3 cut(s) 175, 258, 925
AlwI GGATC 1 cut(s) 272
AoxI GGCC 1 cut(s) 928
ApeKI GCWGC 4 cut(s) 695, 824, 827, 1022
ApoI RAATTY 2 cut(s) 577, 847
AspLEI GCGC 1 cut(s) 507
AspS9I GGNCC 2 cut(s) 588, 956
AsuHPI GGTGA 1 cut(s) 469
AsuII TTCGAA 3 cut(s) 419, 439, 650
AvaII GGWCC 2 cut(s) 588, 956
BbsI GAAGAC 1 cut(s) 408
BbvCI CCTCAGC 4 cut(s) 143, 192, 227, 792
BbvI GCAGC 4 cut(s) 707, 814, 836, 1009
BccI CCATC 3 cut(s) 67, 306, 656
BceAI ACGGC 1 cut(s) 476
BcgI CGANNNNNNTGC 2 cut(s) 409, 443
BciT130I CCWGG 1 cut(s) 960
BclI TGATCA 1 cut(s) 979
BcoDI GTCTC 3 cut(s) 175, 258, 925
BfaI CTAG 2 cut(s) 905, 926
BfmI CTRYAG 1 cut(s) 828
BisI GCNGC 4 cut(s) 696, 825, 828, 1023
BlsI GCNGC 4 cut(s) 697, 826, 829, 1024
Bme1390I CCNGG 1 cut(s) 960
Bme18I GGWCC 2 cut(s) 588, 956
BmgT120I GGNCC 2 cut(s) 588, 956
BmiI GGNNCC 3 cut(s) 324, 589, 590
BmrFI CCNGG 1 cut(s) 960
BmrI ACTGGG 1 cut(s) 69
BmsI GCATC 1 cut(s) 478
BmuI ACTGGG 1 cut(s) 69
BpiI GAAGAC 1 cut(s) 408
BpmI CTGGAG 2 cut(s) 69, 617
Bpu10I CCTNAGC 4 cut(s) 143, 192, 227, 792
Bpu14I TTCGAA 3 cut(s) 419, 439, 650
BsaJI CCNNGG 1 cut(s) 492
BsaXI ACNNNNNCTCC 2 cut(s) 279, 309
Bsc4I CCNNNNNNNGG 1 cut(s) 598
Bse118I RCCGGY 1 cut(s) 1025
Bse1I ACTGG 3 cut(s) 75, 480, 677
BseBI CCWGG 1 cut(s) 960
BseDI CCNNGG 1 cut(s) 492
BseGI GGATG 4 cut(s) 113, 493, 664, 782
BseLI CCNNNNNNNGG 1 cut(s) 598
BseNI ACTGG 3 cut(s) 75, 480, 677
BseRI GAGGAG 1 cut(s) 381
BseXI GCAGC 4 cut(s) 707, 814, 836, 1009
BseYI CCCAGC 3 cut(s) 136, 430, 591
BsgI GTGCAG 3 cut(s) 714, 732, 843
BshFI GGCC 1 cut(s) 930
BsiSI CCGG 2 cut(s) 330, 1026
BslFI GGGAC 4 cut(s) 108, 192, 204, 601
BslI CCNNNNNNNGG 1 cut(s) 598
BsmAI GTCTC 3 cut(s) 175, 258, 925
BsmBI CGTCTC 3 cut(s) 175, 258, 925
BsmFI GGGAC 4 cut(s) 108, 192, 204, 601
BsnI GGCC 1 cut(s) 930
Bsp119I TTCGAA 3 cut(s) 419, 439, 650
Bsp143I GATC 4 cut(s) 178, 277, 460, 979
BspACI CCGC 7 cut(s) 80, 126, 149, 187, 222, 233, 327
BspANI GGCC 1 cut(s) 930
BspLI GGNNCC 3 cut(s) 324, 589, 590
BspMAI CTGCAG 1 cut(s) 832
BspPI GGATC 1 cut(s) 272
BspT104I TTCGAA 3 cut(s) 419, 439, 650
BsrBI CCGCTC 3 cut(s) 128, 151, 235
BsrFI RCCGGY 1 cut(s) 1025
BsrI ACTGG 3 cut(s) 75, 480, 677
BssAI RCCGGY 1 cut(s) 1025
BssECI CCNNGG 1 cut(s) 492
BssMI GATC 4 cut(s) 178, 277, 460, 979
Bst2UI CCWGG 1 cut(s) 960
Bst4CI ACNGT 5 cut(s) 252, 746, 811, 840, 967
Bst6I CTCTTC 3 cut(s) 358, 416, 436
BstBI TTCGAA 3 cut(s) 419, 439, 650
BstC8I GCNNGC 2 cut(s) 149, 233
BstDSI CCRYGG 1 cut(s) 492
BstF5I GGATG 4 cut(s) 113, 493, 664, 782
BstHHI GCGC 1 cut(s) 507
BstKTI GATC 4 cut(s) 181, 280, 463, 982
BstMAI GTCTC 3 cut(s) 175, 258, 925
BstMBI GATC 4 cut(s) 178, 277, 460, 979
BstMWI GCNNNNNNNGC 1 cut(s) 1028
BstNI CCWGG 1 cut(s) 960
BstSCI CCNGG 1 cut(s) 958
BstSFI CTRYAG 1 cut(s) 828
BstV1I GCAGC 4 cut(s) 707, 814, 836, 1009
BstV2I GAAGAC 1 cut(s) 408
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BsuRI GGCC 1 cut(s) 930
BtgI CCRYGG 1 cut(s) 492
BtsCI GGATG 4 cut(s) 113, 493, 664, 782
BtsI GCAGTG 1 cut(s) 837
BtsIMutI CAGTG 2 cut(s) 715, 837
Cac8I GCNNGC 2 cut(s) 149, 233
CfoI GCGC 1 cut(s) 507
Cfr10I RCCGGY 1 cut(s) 1025
Cfr13I GGNCC 2 cut(s) 588, 956
Csp6I GTAC 2 cut(s) 742, 1013
CviAII CATG 2 cut(s) 314, 932
CviQI GTAC 2 cut(s) 742, 1013
DpnI GATC 4 cut(s) 180, 279, 462, 981
DpnII GATC 4 cut(s) 178, 277, 460, 979
Eam1104I CTCTTC 3 cut(s) 358, 416, 436
EarI CTCTTC 3 cut(s) 358, 416, 436
EciI GGCGGA 1 cut(s) 69
Eco47I GGWCC 2 cut(s) 588, 956
Eco57I CTGAAG 2 cut(s) 477, 502
EcoO109I RGGNCCY 1 cut(s) 588
EcoRII CCWGG 1 cut(s) 958
Esp3I CGTCTC 3 cut(s) 175, 258, 925
FaeI CATG 2 cut(s) 317, 935
FalI AAGNNNNNCTT 2 cut(s) 999, 1031
FaqI GGGAC 4 cut(s) 108, 192, 204, 601
FatI CATG 2 cut(s) 313, 931
FauI CCCGC 6 cut(s) 133, 156, 194, 229, 240, 334
FbaI TGATCA 1 cut(s) 979
FblI GTMKAC 1 cut(s) 388
Fnu4HI GCNGC 4 cut(s) 696, 825, 828, 1023
FokI GGATG 4 cut(s) 100, 500, 671, 769
Fsp4HI GCNGC 4 cut(s) 696, 825, 828, 1023
FspAI RTGCGCAY 1 cut(s) 506
FspBI CTAG 2 cut(s) 905, 926
FspI TGCGCA 1 cut(s) 506
GlaI GCGC 1 cut(s) 506
GluI GCNGC 4 cut(s) 696, 825, 828, 1023
GsaI CCCAGC 3 cut(s) 140, 434, 595
GsuI CTGGAG 2 cut(s) 69, 617
HaeIII GGCC 1 cut(s) 930
HapII CCGG 2 cut(s) 330, 1026
HhaI GCGC 1 cut(s) 507
Hin1II CATG 2 cut(s) 317, 935
Hin6I GCGC 1 cut(s) 505
HinP1I GCGC 1 cut(s) 505
HincII GTYRAC 1 cut(s) 389
HindII GTYRAC 1 cut(s) 389
HinfI GANTC 1 cut(s) 635
HpaII CCGG 2 cut(s) 330, 1026
HphI GGTGA 1 cut(s) 469
Hpy166II GTNNAC 3 cut(s) 389, 807, 1001
Hpy188I TCNGA 4 cut(s) 157, 241, 457, 613
Hpy188III TCNNGA 3 cut(s) 176, 320, 759
Hpy8I GTNNAC 3 cut(s) 389, 807, 1001
Hpy99I CGWCG 1 cut(s) 902
HpyAV CCTTC 3 cut(s) 265, 395, 520
HpyCH4III ACNGT 5 cut(s) 252, 746, 811, 840, 967
HpyCH4IV ACGT 1 cut(s) 918
HpyCH4V TGCA 5 cut(s) 695, 713, 824, 830, 1031
HpyF10VI GCNNNNNNNGC 1 cut(s) 1028
HpySE526I ACGT 1 cut(s) 918
Hsp92II CATG 2 cut(s) 317, 935
HspAI GCGC 1 cut(s) 505
KflI GGGWCCC 1 cut(s) 588
Ksp22I TGATCA 1 cut(s) 979
Kzo9I GATC 4 cut(s) 178, 277, 460, 979
Lsp1109I GCAGC 4 cut(s) 707, 814, 836, 1009
LweI GCATC 1 cut(s) 478
MaeI CTAG 2 cut(s) 905, 926
MaeII ACGT 1 cut(s) 918
MaeIII GTNAC 1 cut(s) 475
MalI GATC 4 cut(s) 180, 279, 462, 981
MbiI CCGCTC 3 cut(s) 128, 151, 235
MboI GATC 4 cut(s) 178, 277, 460, 979
MflI RGATCY 1 cut(s) 277
MluCI AATT 3 cut(s) 577, 765, 847
MlyI GAGTC 1 cut(s) 629
MmeI TCCRAC 1 cut(s) 939
MseI TTAA 3 cut(s) 21, 291, 1017
MslI CAYNNNNRTG 2 cut(s) 690, 1047
MspA1I CMGCKG 3 cut(s) 89, 595, 827
MspI CCGG 2 cut(s) 330, 1026
MspR9I CCNGG 1 cut(s) 960
MvaI CCWGG 1 cut(s) 960
MwoI GCNNNNNNNGC 1 cut(s) 1028
NdeII GATC 4 cut(s) 178, 277, 460, 979
NlaIII CATG 2 cut(s) 317, 935
NlaIV GGNNCC 3 cut(s) 324, 589, 590
NmuCI GTSAC 1 cut(s) 475
NsbI TGCGCA 1 cut(s) 506
NspV TTCGAA 3 cut(s) 419, 439, 650
PkrI GCNGC 4 cut(s) 697, 826, 829, 1024
PleI GAGTC 1 cut(s) 629
PpsI GAGTC 1 cut(s) 629
PpuMI RGGWCCY 1 cut(s) 588
Psp5II RGGWCCY 1 cut(s) 588
Psp6I CCWGG 1 cut(s) 958
PspFI CCCAGC 3 cut(s) 136, 430, 591
PspGI CCWGG 1 cut(s) 958
PspN4I GGNNCC 3 cut(s) 324, 589, 590
PspPI GGNCC 2 cut(s) 588, 956
PspPPI RGGWCCY 1 cut(s) 588
PsrI GAACNNNNNNTAC 2 cut(s) 240, 272
PstI CTGCAG 1 cut(s) 832
PsuI RGATCY 1 cut(s) 277
PvuII CAGCTG 3 cut(s) 89, 595, 827
RsaI GTAC 2 cut(s) 743, 1014
RsaNI GTAC 2 cut(s) 742, 1013
RseI CAYNNNNRTG 2 cut(s) 690, 1047
SalI GTCGAC 1 cut(s) 387
SaqAI TTAA 3 cut(s) 21, 291, 1017
SatI GCNGC 4 cut(s) 696, 825, 828, 1023
Sau3AI GATC 4 cut(s) 178, 277, 460, 979
Sau96I GGNCC 2 cut(s) 588, 956
SchI GAGTC 1 cut(s) 629
ScrFI CCNGG 1 cut(s) 960
SfaNI GCATC 1 cut(s) 478
SfcI CTRYAG 1 cut(s) 828
SfuI TTCGAA 3 cut(s) 419, 439, 650
SinI GGWCC 2 cut(s) 588, 956
SmiMI CAYNNNNRTG 2 cut(s) 690, 1047
Sse9I AATT 3 cut(s) 577, 765, 847
SsiI CCGC 7 cut(s) 80, 126, 149, 187, 222, 233, 327
SspI AATATT 1 cut(s) 862
SspMI CTAG 2 cut(s) 905, 926
StyD4I CCNGG 1 cut(s) 958
TaaI ACNGT 5 cut(s) 252, 746, 811, 840, 967
TaiI ACGT 1 cut(s) 921
TaqI TCGA 7 cut(s) 62, 177, 388, 419, 439, 650, 900
TasI AATT 3 cut(s) 577, 765, 847
TatI WGTACW 2 cut(s) 741, 1012
Tru1I TTAA 3 cut(s) 21, 291, 1017
Tru9I TTAA 3 cut(s) 21, 291, 1017
TscAI CASTG 2 cut(s) 715, 837
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 4 cut(s) 695, 824, 827, 1022
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 4 cut(s) 302, 502, 674, 771
TspRI CASTG 2 cut(s) 715, 837
VpaK11BI GGWCC 2 cut(s) 588, 956
XapI RAATTY 2 cut(s) 577, 847
XcmI CCANNNNNNNNNTGG 1 cut(s) 628
XmiI GTMKAC 1 cut(s) 388
XspI CTAG 2 cut(s) 905, 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.