Rmu_co8187766.1_g000001

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8187766.1
OG0020383
Physical Location & Seq
Reverse (-)
1 .. 655
655 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8187766.1_g000001.1.cds

Sequence Viewer

Length: 477 bp
atgtaccagaagaagagccagctggagcacatcttgctccgacccgatacctacatcggatccatagagaagcacacccaaaccctctgggtcttcgagaacgacgagatggtccagcgctccgtctcctacgtccccggcctctacaagatcttcgacgagattctggtcaacgccgccgacaacaagcagcgagatgcgtccatggacgctctcaaggtcaccatcgacgtcgagcagaactgcatcagtgtgtacaacaacggcgacggagttccggtggagatccaccaggaggagagggtttatgtgccggagttgatcttcgggcacctactgactagtagtaactacgacgacacggtgaagaagaccaccggtggacgcaacggctacggcgccaagctcactaatatattctccaccgaattcgtcatcgaaaccgccgacggcaagcggcagaagaagtacaagcag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01524 map01524
Pfam Domains
Protein Families
DNA_gyraseB DNA_topoisoIV HATPase_c TOPRIM_C Toprim

Protein Analysis

159

Amino Acids

18.21

Weight (kDa)

5.57

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020383)

Species Orthologous Gene IDs
malus_domestica MD01G1165800.v1.1
prunus_persica Prupe.2G018500_v2.0.a1
pyrus_communis pycom11g13880
rosa_multiflora Rmu_co8187766.1_g000001
rosa_samantha Rh1CG375500 Rh1DG393000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
AatII GACGTC 1 cut(s) 234
AccB1I GGYRCC 2 cut(s) 330, 398
AciI CCGC 3 cut(s) 177, 444, 457
AclWI GGATC 3 cut(s) 54, 67, 280
AcsI RAATTY 1 cut(s) 428
AcyI GRCGYC 2 cut(s) 231, 399
AfaI GTAC 3 cut(s) 5, 257, 470
AfeI AGCGCT 1 cut(s) 119
AfiI CCNNNNNNNGG 1 cut(s) 295
AgeI ACCGGT 1 cut(s) 377
AhlI ACTAGT 1 cut(s) 341
AjnI CCWGG 1 cut(s) 291
AluBI AGCT 2 cut(s) 22, 406
AluI AGCT 2 cut(s) 22, 406
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 130
AlwI GGATC 3 cut(s) 54, 67, 280
Aor51HI AGCGCT 1 cut(s) 119
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 1 cut(s) 190
ApoI RAATTY 1 cut(s) 428
AsiGI ACCGGT 1 cut(s) 377
AspLEI GCGC 2 cut(s) 120, 401
AspS9I GGNCC 1 cut(s) 112
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 2 cut(s) 214, 376
AvaII GGWCC 1 cut(s) 112
BaeGI GKGCMC 1 cut(s) 333
BamHI GGATCC 1 cut(s) 59
BanI GGYRCC 2 cut(s) 330, 398
BbsI GAAGAC 2 cut(s) 85, 377
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 202
BccI CCATC 2 cut(s) 103, 233
BceAI ACGGC 4 cut(s) 280, 406, 412, 466
BciT130I CCWGG 1 cut(s) 293
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 130
BcuI ACTAGT 1 cut(s) 341
BfaI CTAG 1 cut(s) 342
BfoI RGCGCY 2 cut(s) 121, 402
BglII AGATCT 1 cut(s) 150
BisI GCNGC 3 cut(s) 177, 191, 458
BlsI GCNGC 3 cut(s) 178, 192, 459
Bme1390I CCNGG 2 cut(s) 138, 293
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 3 cut(s) 61, 332, 400
BmrFI CCNGG 2 cut(s) 138, 293
BmsI GCATC 2 cut(s) 187, 255
BpiI GAAGAC 2 cut(s) 85, 377
BpmI CTGGAG 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 200
BpuMI CCSGG 1 cut(s) 138
BsaHI GRCGYC 2 cut(s) 231, 399
BsaJI CCNNGG 2 cut(s) 136, 204
BsaWI WCCGGW 2 cut(s) 277, 377
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bsc4I CCNNNNNNNGG 1 cut(s) 295
Bse118I RCCGGY 1 cut(s) 377
BseBI CCWGG 1 cut(s) 293
BseDI CCNNGG 2 cut(s) 136, 204
BseLI CCNNNNNNNGG 1 cut(s) 295
BseRI GAGGAG 1 cut(s) 311
BseSI GKGCMC 1 cut(s) 333
BseXI GCAGC 1 cut(s) 202
BshFI GGCC 1 cut(s) 141
BshNI GGYRCC 2 cut(s) 330, 398
BshTI ACCGGT 1 cut(s) 377
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 4 cut(s) 138, 278, 314, 378
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 1 cut(s) 295
BsmAI GTCTC 1 cut(s) 130
BsmBI CGTCTC 1 cut(s) 130
BsmFI GGGAC 1 cut(s) 119
BsnI GGCC 1 cut(s) 141
Bsp1286I GDGCHC 2 cut(s) 30, 333
Bsp1407I TGTACA 1 cut(s) 255
Bsp143I GATC 4 cut(s) 59, 150, 285, 321
Bsp19I CCATGG 1 cut(s) 204
BspACI CCGC 3 cut(s) 177, 444, 457
BspANI GGCC 1 cut(s) 141
BspLI GGNNCC 3 cut(s) 61, 332, 400
BspPI GGATC 3 cut(s) 54, 67, 280
BspQI GCTCTTC 1 cut(s) 8
BspT107I GGYRCC 2 cut(s) 330, 398
BsrFI RCCGGY 1 cut(s) 377
BsrGI TGTACA 1 cut(s) 255
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 2 cut(s) 136, 204
BssMI GATC 4 cut(s) 59, 150, 285, 321
BssNI GRCGYC 2 cut(s) 231, 399
BssT1I CCWWGG 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 1 cut(s) 364
Bst6I CTCTTC 1 cut(s) 8
BstACI GRCGYC 2 cut(s) 231, 399
BstAPI GCANNNNNTGC 1 cut(s) 34
BstAUI TGTACA 1 cut(s) 255
BstC8I GCNNGC 2 cut(s) 20, 455
BstDSI CCRYGG 1 cut(s) 204
BstEII GGTNACC 1 cut(s) 220
BstH2I RGCGCY 2 cut(s) 121, 402
BstHHI GCGC 2 cut(s) 120, 401
BstKTI GATC 4 cut(s) 62, 153, 288, 324
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 4 cut(s) 59, 150, 285, 321
BstMWI GCNNNNNNNGC 1 cut(s) 34
BstNI CCWGG 1 cut(s) 293
BstPI GGTNACC 1 cut(s) 220
BstSCI CCNGG 2 cut(s) 136, 291
BstSLI GKGCMC 1 cut(s) 333
BstV1I GCAGC 1 cut(s) 202
BstV2I GAAGAC 2 cut(s) 85, 377
BstX2I RGATCY 3 cut(s) 59, 150, 285
BstYI RGATCY 3 cut(s) 59, 150, 285
BsuRI GGCC 1 cut(s) 141
BtgI CCRYGG 1 cut(s) 204
BtsIMutI CAGTG 1 cut(s) 256
Cac8I GCNNGC 2 cut(s) 20, 455
CfoI GCGC 2 cut(s) 120, 401
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 1 cut(s) 112
CseI GACGC 3 cut(s) 189, 218, 393
Csp6I GTAC 3 cut(s) 4, 256, 469
CspAI ACCGGT 1 cut(s) 377
CviAII CATG 1 cut(s) 205
CviJI RGCY 5 cut(s) 18, 22, 141, 393, 406
CviKI_1 RGCY 5 cut(s) 18, 22, 141, 393, 406
CviQI GTAC 3 cut(s) 4, 256, 469
DinI GGCGCC 1 cut(s) 400
DpnI GATC 4 cut(s) 61, 152, 287, 323
DpnII GATC 4 cut(s) 59, 150, 285, 321
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
Eco130I CCWWGG 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 112
Eco47III AGCGCT 1 cut(s) 119
Eco91I GGTNACC 1 cut(s) 220
EcoO65I GGTNACC 1 cut(s) 220
EcoRI GAATTC 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 291
EcoT14I CCWWGG 1 cut(s) 204
EgeI GGCGCC 1 cut(s) 400
EheI GGCGCC 1 cut(s) 400
ErhI CCWWGG 1 cut(s) 204
Esp3I CGTCTC 1 cut(s) 130
FaeI CATG 1 cut(s) 208
FaiI YATR 4 cut(s) 65, 206, 309, 416
FaqI GGGAC 1 cut(s) 119
FatI CATG 1 cut(s) 204
Fnu4HI GCNGC 3 cut(s) 177, 191, 458
Fsp4HI GCNGC 3 cut(s) 177, 191, 458
FspBI CTAG 1 cut(s) 342
GlaI GCGC 2 cut(s) 119, 400
GluI GCNGC 3 cut(s) 177, 191, 458
GsuI CTGGAG 1 cut(s) 44
HaeII RGCGCY 2 cut(s) 121, 402
HaeIII GGCC 1 cut(s) 141
HapII CCGG 4 cut(s) 138, 278, 314, 378
HgaI GACGC 3 cut(s) 189, 218, 393
HhaI GCGC 2 cut(s) 120, 401
Hin1I GRCGYC 2 cut(s) 231, 399
Hin1II CATG 1 cut(s) 208
Hin6I GCGC 2 cut(s) 118, 399
HinP1I GCGC 2 cut(s) 118, 399
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 1 cut(s) 163
HpaII CCGG 4 cut(s) 138, 278, 314, 378
HphI GGTGA 2 cut(s) 214, 376
Hpy166II GTNNAC 3 cut(s) 172, 256, 383
Hpy188I TCNGA 2 cut(s) 41, 59
Hpy188III TCNNGA 1 cut(s) 97
Hpy8I GTNNAC 3 cut(s) 172, 256, 383
Hpy99I CGWCG 7 cut(s) 107, 161, 233, 236, 272, 359, 452
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 2 cut(s) 132, 231
HpyCH4V TGCA 1 cut(s) 246
HpyF10VI GCNNNNNNNGC 1 cut(s) 34
HpySE526I ACGT 2 cut(s) 132, 231
Hsp92I GRCGYC 2 cut(s) 231, 399
Hsp92II CATG 1 cut(s) 208
HspAI GCGC 2 cut(s) 118, 399
KasI GGCGCC 1 cut(s) 398
Kzo9I GATC 4 cut(s) 59, 150, 285, 321
LguI GCTCTTC 1 cut(s) 8
LmnI GCTCC 3 cut(s) 25, 42, 125
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 2 cut(s) 187, 255
MaeI CTAG 1 cut(s) 342
MaeII ACGT 2 cut(s) 132, 231
MaeIII GTNAC 2 cut(s) 220, 347
MalI GATC 4 cut(s) 61, 152, 287, 323
MboI GATC 4 cut(s) 59, 150, 285, 321
MboII GAAGA 8 cut(s) 22, 25, 85, 145, 316, 379, 382, 475
MflI RGATCY 3 cut(s) 59, 150, 285
MhlI GDGCHC 2 cut(s) 30, 333
MluCI AATT 1 cut(s) 428
Mly113I GGCGCC 1 cut(s) 399
MmeI TCCRAC 1 cut(s) 64
MnlI CCTC 4 cut(s) 95, 152, 289, 294
MslI CAYNNNNRTG 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 22
MspI CCGG 4 cut(s) 138, 278, 314, 378
MspR9I CCNGG 2 cut(s) 138, 293
MvaI CCWGG 1 cut(s) 293
MwoI GCNNNNNNNGC 1 cut(s) 34
NarI GGCGCC 1 cut(s) 399
NciI CCSGG 1 cut(s) 138
NcoI CCATGG 1 cut(s) 204
NdeII GATC 4 cut(s) 59, 150, 285, 321
NlaIII CATG 1 cut(s) 208
NlaIV GGNNCC 3 cut(s) 61, 332, 400
NmuCI GTSAC 1 cut(s) 220
PciSI GCTCTTC 1 cut(s) 8
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 444
PfeI GAWTC 1 cut(s) 163
PinAI ACCGGT 1 cut(s) 377
PkrI GCNGC 3 cut(s) 178, 192, 459
PluTI GGCGCC 1 cut(s) 402
Psp6I CCWGG 1 cut(s) 291
PspEI GGTNACC 1 cut(s) 220
PspGI CCWGG 1 cut(s) 291
PspN4I GGNNCC 3 cut(s) 61, 332, 400
PspPI GGNCC 1 cut(s) 112
PsuI RGATCY 3 cut(s) 59, 150, 285
PvuII CAGCTG 1 cut(s) 22
RsaI GTAC 3 cut(s) 5, 257, 470
RsaNI GTAC 3 cut(s) 4, 256, 469
RseI CAYNNNNRTG 1 cut(s) 251
SapI GCTCTTC 1 cut(s) 8
SatI GCNGC 3 cut(s) 177, 191, 458
Sau3AI GATC 4 cut(s) 59, 150, 285, 321
Sau96I GGNCC 1 cut(s) 112
ScrFI CCNGG 2 cut(s) 138, 293
SduI GDGCHC 2 cut(s) 30, 333
SetI ASST 7 cut(s) 24, 53, 135, 222, 234, 336, 408
SfaNI GCATC 2 cut(s) 187, 255
SfoI GGCGCC 1 cut(s) 400
SgrAI CRCCGGYG 1 cut(s) 377
SinI GGWCC 1 cut(s) 112
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SpeI ACTAGT 1 cut(s) 341
Sse9I AATT 1 cut(s) 428
SsiI CCGC 3 cut(s) 177, 444, 457
SspDI GGCGCC 1 cut(s) 398
SspMI CTAG 1 cut(s) 342
StyD4I CCNGG 2 cut(s) 136, 291
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 2 cut(s) 135, 234
TaqI TCGA 5 cut(s) 96, 156, 228, 234, 438
TasI AATT 1 cut(s) 428
TatI WGTACW 2 cut(s) 255, 468
TauI GCSGC 2 cut(s) 179, 460
TfiI GAWTC 1 cut(s) 163
TscAI CASTG 1 cut(s) 256
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 1 cut(s) 190
Tsp45I GTSAC 1 cut(s) 220
TspGWI ACGGA 2 cut(s) 112, 285
TspRI CASTG 1 cut(s) 256
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 1 cut(s) 428
XspI CTAG 1 cut(s) 342
ZraI GACGTC 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.