Rmu_sc0000117.1_g000024

Domain of unknown function (DUF4216)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000117.1
OG0023031
Physical Location & Seq
Reverse (-)
109428 .. 111861
2434 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 789 bp
atgagcgaactcaactcttacagtcccgtcgcacatgagctaaacaatcaagttatgacttataagtttgggttaagtaagttagagttccttcctctaacctatactcggggggactatcatggtcacgccatagtctcgctaatactgcaaatagttacttcgataccgaagctctattctcagccccacaggtataacctagatgatgggcccttgtggcaccaacctgccatggaagacttcccaagacccgaagagtccattcttgcttacggagttggaggactagtatggggcatgcatctggtagatcgacacctgagctacattgccatggtagagggtgggcctcatgacatggctagcccagcctacattgccggagtggtgtttcattacggccaaagtggggtgaccttaaatgcagatgcattgctagtttctagtgccaaagataggaattctatagatgatgagatgactttctacagagttgcaaacagtaagaaggggataaaagtagaggattttggattcaccttagttgatcttagtaggatggggcacaagtttgattcctttgttttagctgatcttttgcaaaaggtgttaaagttatgggatccagaagaagaaaatcatgatactgaagatgacttgtcatctgatgagaagaaagggagaggtccaactctgatgtccgacattattcatagtaggagtaagggtaggcctcatcaaaaagcccgcggatcaagtgggccgatggaggcccgccggccgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218

Protein Analysis

262

Amino Acids

29.37

Weight (kDa)

5.88

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023031)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000117.1_g000024
rosa_roxburghii Rroxscaffold_4G00324600 Rroxscaffold_5G00338620 Rroxscaffold_5G00382600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 63
Acc36I ACCTGC 1 cut(s) 238
AccB1I GGYRCC 1 cut(s) 222
AccII CGCG 1 cut(s) 753
AciI CCGC 3 cut(s) 751, 753, 778
AclWI GGATC 3 cut(s) 620, 633, 763
AcoI YGGCCR 2 cut(s) 403, 782
AcsI RAATTY 1 cut(s) 463
AcuI CTGAAG 1 cut(s) 672
AfiI CCNNNNNNNGG 3 cut(s) 108, 412, 459
AhlI ACTAGT 1 cut(s) 289
AluBI AGCT 4 cut(s) 40, 175, 327, 593
AluI AGCT 4 cut(s) 40, 175, 327, 593
Alw26I GTCTC 1 cut(s) 142
AlwI GGATC 3 cut(s) 620, 633, 763
Ama87I CYCGRG 1 cut(s) 108
AoxI GGCC 7 cut(s) 212, 350, 403, 734, 764, 774, 782
ApaI GGGCCC 1 cut(s) 216
ApoI RAATTY 1 cut(s) 463
AspS9I GGNCC 6 cut(s) 212, 213, 350, 689, 764, 775
AsuHPI GGTGA 2 cut(s) 427, 532
AsuNHI GCTAGC 1 cut(s) 365
AvaI CYCGRG 1 cut(s) 108
AvaII GGWCC 1 cut(s) 689
BaeGI GKGCMC 2 cut(s) 216, 570
BamHI GGATCC 1 cut(s) 625
BanI GGYRCC 1 cut(s) 222
BanII GRGCYC 1 cut(s) 216
BbsI GAAGAC 1 cut(s) 246
BccI CCATC 3 cut(s) 203, 556, 763
BceAI ACGGC 2 cut(s) 418, 769
BcoDI GTCTC 1 cut(s) 142
BcuI ACTAGT 1 cut(s) 289
BfaI CTAG 5 cut(s) 203, 290, 366, 440, 447
BfmI CTRYAG 2 cut(s) 468, 490
BfuAI ACCTGC 1 cut(s) 238
BglI GCCNNNNNGGC 1 cut(s) 220
Bme18I GGWCC 1 cut(s) 689
BmeT110I CYCGRG 1 cut(s) 108
BmgT120I GGNCC 6 cut(s) 212, 213, 350, 689, 764, 775
BmiI GGNNCC 3 cut(s) 214, 224, 627
BmsI GCATC 2 cut(s) 313, 421
BmtI GCTAGC 1 cut(s) 369
BpiI GAAGAC 1 cut(s) 246
Bpu10I CCTNAGC 1 cut(s) 323
BsaJI CCNNGG 3 cut(s) 234, 336, 751
BsaXI ACNNNNNCTCC 4 cut(s) 276, 306, 715, 745
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 412, 459
Bse118I RCCGGY 1 cut(s) 780
Bse3DI GCAATG 3 cut(s) 330, 378, 434
BseDI CCNNGG 3 cut(s) 234, 336, 751
BseGI GGATG 1 cut(s) 567
BseLI CCNNNNNNNGG 3 cut(s) 108, 412, 459
BseMI GCAATG 3 cut(s) 330, 378, 434
BseMII CTCAG 2 cut(s) 197, 314
BseSI GKGCMC 2 cut(s) 216, 570
BseX3I CGGCCG 1 cut(s) 782
BseYI CCCAGC 1 cut(s) 370
Bsh1236I CGCG 1 cut(s) 753
Bsh1285I CGRYCG 1 cut(s) 785
BshFI GGCC 7 cut(s) 214, 352, 405, 736, 766, 776, 784
BshNI GGYRCC 1 cut(s) 222
BsiEI CGRYCG 1 cut(s) 785
BsiHKCI CYCGRG 1 cut(s) 108
BsiSI CCGG 2 cut(s) 384, 781
BslFI GGGAC 2 cut(s) 9, 128
BslI CCNNNNNNNGG 3 cut(s) 108, 412, 459
BsmAI GTCTC 1 cut(s) 142
BsmFI GGGAC 2 cut(s) 9, 128
BsnI GGCC 7 cut(s) 214, 352, 405, 736, 766, 776, 784
BsoBI CYCGRG 1 cut(s) 108
Bsp120I GGGCCC 1 cut(s) 212
Bsp1286I GDGCHC 2 cut(s) 216, 570
Bsp143I GATC 5 cut(s) 313, 550, 595, 625, 755
Bsp19I CCATGG 2 cut(s) 234, 336
BspACI CCGC 3 cut(s) 751, 753, 778
BspANI GGCC 7 cut(s) 214, 352, 405, 736, 766, 776, 784
BspCNI CTCAG 2 cut(s) 196, 315
BspFNI CGCG 1 cut(s) 753
BspHI TCATGA 2 cut(s) 355, 643
BspLI GGNNCC 3 cut(s) 214, 224, 627
BspMI ACCTGC 1 cut(s) 238
BspOI GCTAGC 1 cut(s) 369
BspPI GGATC 3 cut(s) 620, 633, 763
BspT107I GGYRCC 1 cut(s) 222
BsrDI GCAATG 3 cut(s) 330, 378, 434
BsrFI RCCGGY 1 cut(s) 780
BssAI RCCGGY 1 cut(s) 780
BssECI CCNNGG 3 cut(s) 234, 336, 751
BssMI GATC 5 cut(s) 313, 550, 595, 625, 755
BssT1I CCWWGG 2 cut(s) 234, 336
Bst4CI ACNGT 2 cut(s) 23, 506
Bst6I CTCTTC 1 cut(s) 252
BstC8I GCNNGC 5 cut(s) 302, 367, 751, 778, 782
BstDEI CTNAG 4 cut(s) 183, 323, 544, 554
BstDSI CCRYGG 3 cut(s) 234, 336, 751
BstEII GGTNACC 1 cut(s) 415
BstF5I GGATG 1 cut(s) 567
BstFNI CGCG 1 cut(s) 753
BstKTI GATC 5 cut(s) 316, 553, 598, 628, 758
BstMAI GTCTC 1 cut(s) 142
BstMBI GATC 5 cut(s) 313, 550, 595, 625, 755
BstMCI CGRYCG 1 cut(s) 785
BstMWI GCNNNNNNNGC 4 cut(s) 148, 220, 371, 380
BstNSI RCATGY 1 cut(s) 304
BstPI GGTNACC 1 cut(s) 415
BstSFI CTRYAG 2 cut(s) 468, 490
BstSLI GKGCMC 2 cut(s) 216, 570
BstUI CGCG 1 cut(s) 753
BstV2I GAAGAC 1 cut(s) 246
BstX2I RGATCY 1 cut(s) 625
BstYI RGATCY 1 cut(s) 625
BstZI CGGCCG 1 cut(s) 782
BsuRI GGCC 7 cut(s) 214, 352, 405, 736, 766, 776, 784
BtgI CCRYGG 3 cut(s) 234, 336, 751
BtsCI GGATG 1 cut(s) 567
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 5 cut(s) 302, 367, 751, 778, 782
CciI TCATGA 2 cut(s) 355, 643
Cfr10I RCCGGY 1 cut(s) 780
Cfr13I GGNCC 6 cut(s) 212, 213, 350, 689, 764, 775
Cfr42I CCGCGG 1 cut(s) 754
CviAII CATG 8 cut(s) 35, 122, 235, 301, 337, 356, 361, 644
DdeI CTNAG 4 cut(s) 183, 323, 544, 554
DpnI GATC 5 cut(s) 315, 552, 597, 627, 757
DpnII GATC 5 cut(s) 313, 550, 595, 625, 755
EaeI YGGCCR 2 cut(s) 403, 782
EagI CGGCCG 1 cut(s) 782
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
EclXI CGGCCG 1 cut(s) 782
Eco130I CCWWGG 2 cut(s) 234, 336
Eco147I AGGCCT 1 cut(s) 736
Eco24I GRGCYC 1 cut(s) 216
Eco47I GGWCC 1 cut(s) 689
Eco52I CGGCCG 1 cut(s) 782
Eco57I CTGAAG 1 cut(s) 672
Eco88I CYCGRG 1 cut(s) 108
Eco91I GGTNACC 1 cut(s) 415
EcoO109I RGGNCCY 1 cut(s) 213
EcoO65I GGTNACC 1 cut(s) 415
EcoRI GAATTC 1 cut(s) 463
EcoT14I CCWWGG 2 cut(s) 234, 336
EcoT22I ATGCAT 2 cut(s) 306, 436
EcoT38I GRGCYC 1 cut(s) 216
ErhI CCWWGG 2 cut(s) 234, 336
FaeI CATG 8 cut(s) 38, 125, 238, 304, 340, 359, 364, 647
FaqI GGGAC 2 cut(s) 9, 128
FatI CATG 8 cut(s) 34, 121, 234, 300, 336, 355, 360, 643
FauI CCCGC 2 cut(s) 758, 785
FokI GGATG 1 cut(s) 574
FriOI GRGCYC 1 cut(s) 216
FspBI CTAG 5 cut(s) 203, 290, 366, 440, 447
GsaI CCCAGC 1 cut(s) 374
HaeIII GGCC 7 cut(s) 214, 352, 405, 736, 766, 776, 784
HapII CCGG 2 cut(s) 384, 781
Hin1II CATG 8 cut(s) 38, 125, 238, 304, 340, 359, 364, 647
HinfI GANTC 3 cut(s) 260, 537, 578
HpaII CCGG 2 cut(s) 384, 781
HphI GGTGA 2 cut(s) 427, 532
Hpy188I TCNGA 3 cut(s) 670, 699, 706
Hpy188III TCNNGA 3 cut(s) 356, 629, 644
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 2 cut(s) 101, 505
HpyCH4III ACNGT 2 cut(s) 23, 506
HpyCH4V TGCA 6 cut(s) 151, 304, 428, 434, 500, 604
HpyF10VI GCNNNNNNNGC 4 cut(s) 148, 220, 371, 380
HpyF3I CTNAG 4 cut(s) 183, 323, 544, 554
Hsp92II CATG 8 cut(s) 38, 125, 238, 304, 340, 359, 364, 647
KroI GCCGGC 1 cut(s) 780
KroNI GCCGGC 1 cut(s) 782
KspI CCGCGG 1 cut(s) 754
Kzo9I GATC 5 cut(s) 313, 550, 595, 625, 755
LpnPI CCDG 7 cut(s) 178, 243, 293, 335, 384, 397, 642
LweI GCATC 2 cut(s) 313, 421
MaeI CTAG 5 cut(s) 203, 290, 366, 440, 447
MaeIII GTNAC 3 cut(s) 125, 157, 415
MalI GATC 5 cut(s) 315, 552, 597, 627, 757
MboI GATC 5 cut(s) 313, 550, 595, 625, 755
MboII GAAGA 6 cut(s) 251, 269, 644, 647, 665, 688
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 2 cut(s) 216, 570
MluCI AATT 1 cut(s) 463
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 3 cut(s) 262, 716, 729
MnlI CCTC 8 cut(s) 105, 278, 337, 363, 520, 680, 747, 766
Mph1103I ATGCAT 2 cut(s) 306, 436
MroNI GCCGGC 1 cut(s) 780
MseI TTAA 3 cut(s) 74, 422, 614
MslI CAYNNNNRTG 1 cut(s) 335
MspA1I CMGCKG 1 cut(s) 753
MspI CCGG 2 cut(s) 384, 781
MvnI CGCG 1 cut(s) 753
MwoI GCNNNNNNNGC 4 cut(s) 148, 220, 371, 380
NaeI GCCGGC 1 cut(s) 782
NcoI CCATGG 2 cut(s) 234, 336
NdeII GATC 5 cut(s) 313, 550, 595, 625, 755
NgoMIV GCCGGC 1 cut(s) 780
NheI GCTAGC 1 cut(s) 365
NlaIII CATG 8 cut(s) 38, 125, 238, 304, 340, 359, 364, 647
NlaIV GGNNCC 3 cut(s) 214, 224, 627
NmuCI GTSAC 2 cut(s) 125, 415
NsiI ATGCAT 2 cut(s) 306, 436
NspI RCATGY 1 cut(s) 304
PaeI GCATGC 1 cut(s) 304
PagI TCATGA 2 cut(s) 355, 643
PceI AGGCCT 1 cut(s) 736
PdiI GCCGGC 1 cut(s) 782
PfeI GAWTC 2 cut(s) 537, 578
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
PsiI TTATAA 1 cut(s) 63
PspEI GGTNACC 1 cut(s) 415
PspFI CCCAGC 1 cut(s) 370
PspN4I GGNNCC 3 cut(s) 214, 224, 627
PspOMI GGGCCC 1 cut(s) 212
PspPI GGNCC 6 cut(s) 212, 213, 350, 689, 764, 775
PsuI RGATCY 1 cut(s) 625
RseI CAYNNNNRTG 1 cut(s) 335
SacII CCGCGG 1 cut(s) 754
SaqAI TTAA 3 cut(s) 74, 422, 614
Sau3AI GATC 5 cut(s) 313, 550, 595, 625, 755
Sau96I GGNCC 6 cut(s) 212, 213, 350, 689, 764, 775
SchI GAGTC 1 cut(s) 269
SduI GDGCHC 2 cut(s) 216, 570
SfaNI GCATC 2 cut(s) 313, 421
SfcI CTRYAG 2 cut(s) 468, 490
Sfr303I CCGCGG 1 cut(s) 754
SgrBI CCGCGG 1 cut(s) 754
SinI GGWCC 1 cut(s) 689
SmiMI CAYNNNNRTG 1 cut(s) 335
SpeI ACTAGT 1 cut(s) 289
SphI GCATGC 1 cut(s) 304
Sse9I AATT 1 cut(s) 463
SseBI AGGCCT 1 cut(s) 736
SsiI CCGC 3 cut(s) 751, 753, 778
SspMI CTAG 5 cut(s) 203, 290, 366, 440, 447
StuI AGGCCT 1 cut(s) 736
StyI CCWWGG 2 cut(s) 234, 336
TaaI ACNGT 2 cut(s) 23, 506
TaqI TCGA 2 cut(s) 164, 316
TasI AATT 1 cut(s) 463
TfiI GAWTC 2 cut(s) 537, 578
Tru1I TTAA 3 cut(s) 74, 422, 614
Tru9I TTAA 3 cut(s) 74, 422, 614
TseFI GTSAC 2 cut(s) 125, 415
Tsp45I GTSAC 2 cut(s) 125, 415
TspDTI ATGAA 2 cut(s) 386, 704
TspGWI ACGGA 1 cut(s) 291
VpaK11BI GGWCC 1 cut(s) 689
XapI RAATTY 1 cut(s) 463
XceI RCATGY 1 cut(s) 304
XspI CTAG 5 cut(s) 203, 290, 366, 440, 447
Zsp2I ATGCAT 2 cut(s) 306, 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.