Rroxscaffold_5G00338620

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0023031
Physical Location & Seq
Reverse (-)
6833492 .. 6837172
3681 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00338620.1

Sequence Viewer

Length: 864 bp
ATGGACACCCAGCCCAAACACCAGAAAATTTCTCGCTTCAGCTACGTGAAGATCGGTCTCGCTCGACCTCTAGTCATCACCCTCACCCTTGCCAACTTGAACTGGCCCCCGTCACTTCCCGGTCAAACACTGACCGACGAGCATGCTCTGGGAGTCCGGGTACATACGGAACAGCTGCATCAGATTTTCGCAGCTCCAGCCATGAACGAGCTCCGGGTACATACGGCTTCAATTGAACTGCTACCTCACCGGCCACGCTCAAGCCTATCTACCCCACCGGTATATGAGGCCTACGAGGCCCTCATAAATCCTCTACTCAAGCGAGGTATCCCAACGGGATCACTAAAGGGTAGCTCCCTTACAAAAGCCAAGGTCCAATCCCTTGCAACCAAATCCTTGCCCGGGCGAGATACCACCAAGTCCTCATTTACCCTATCGGGTACGAGAGGCATCAAGCCCTCATGCTACCCCATCGGGTACGAGAGGCATCAAGCCCTCATGCCACCCCGTCGGGTACGAGAGGCATCAAGTCCTCATTCTACCCTTCGGGTATCGAGAGGCATCAAGCCCTCATGCTACCCTTCCGGCTATCGAGAGGCATCAAGCCCTCAACCTACCCCATTCCGGTGCCGAGGCTCAAGTCCTCAACCTATCCTTCGGCTTCATGAGGGCCCGGGTACCAACATACCCGTAGAGTCCCTTCTTACTTACGGAGTTGGGGGACTAGTATGGGTATGGCGTTCAACAAGGTCATCACTCATACTTGGCGGCATCATACTTAAACTCCCCAACCAAGAAGCGCCTCTTACTCGGGGACTTGGGGGACTTGTACATACCGCCCATATTACATACCGCCAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

287

Amino Acids

31.4

Weight (kDa)

10.45

Isoelectric Point (pI)

49.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023031)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000117.1_g000024
rosa_roxburghii Rroxscaffold_4G00324600 Rroxscaffold_5G00338620 Rroxscaffold_5G00382600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 677
AccB1I GGYRCC 2 cut(s) 627, 677
AciI CCGC 3 cut(s) 768, 837, 853
AclWI GGATC 1 cut(s) 346
AcoI YGGCCR 1 cut(s) 251
AcsI RAATTY 1 cut(s) 27
AcuI CTGAAG 1 cut(s) 22
AfaI GTAC 7 cut(s) 162, 219, 442, 479, 516, 679, 831
AfiI CCNNNNNNNGG 2 cut(s) 402, 624
AgeI ACCGGT 1 cut(s) 277
AgsI TTSAA 4 cut(s) 100, 231, 236, 744
AhdI GACNNNNNGTC 1 cut(s) 71
AhlI ACTAGT 1 cut(s) 724
AluBI AGCT 5 cut(s) 42, 175, 194, 211, 354
AluI AGCT 5 cut(s) 42, 175, 194, 211, 354
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 1 cut(s) 62
AlwI GGATC 1 cut(s) 346
Ama87I CYCGRG 3 cut(s) 401, 673, 810
AoxI GGCC 5 cut(s) 104, 251, 288, 297, 670
ApaI GGGCCC 1 cut(s) 674
ApeKI GCWGC 2 cut(s) 175, 191
ApoI RAATTY 1 cut(s) 27
AsiGI ACCGGT 1 cut(s) 277
Asp718I GGTACC 1 cut(s) 677
AspLEI GCGC 1 cut(s) 802
AspS9I GGNCC 5 cut(s) 105, 298, 373, 670, 671
AsuC2I CCSGG 7 cut(s) 120, 158, 215, 402, 403, 674, 675
AsuHPI GGTGA 3 cut(s) 70, 76, 239
AvaI CYCGRG 3 cut(s) 401, 673, 810
AvaII GGWCC 1 cut(s) 373
BaeGI GKGCMC 1 cut(s) 674
BanI GGYRCC 2 cut(s) 627, 677
BanII GRGCYC 2 cut(s) 213, 674
BarI GAAGNNNNNNTAC 2 cut(s) 684, 716
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 2 cut(s) 162, 203
BccI CCATC 1 cut(s) 479
BceAI ACGGC 1 cut(s) 240
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BciVI GTATCC 1 cut(s) 338
BcnI CCSGG 7 cut(s) 120, 158, 215, 402, 403, 674, 675
BcoDI GTCTC 1 cut(s) 62
BcuI ACTAGT 1 cut(s) 724
BfaI CTAG 2 cut(s) 71, 725
BfoI RGCGCY 1 cut(s) 803
BfuI GTATCC 1 cut(s) 338
BglI GCCNNNNNGGC 1 cut(s) 296
BisI GCNGC 3 cut(s) 176, 192, 769
BlsI GCNGC 3 cut(s) 177, 193, 770
Bme1390I CCNGG 7 cut(s) 120, 158, 215, 402, 403, 674, 675
Bme18I GGWCC 1 cut(s) 373
BmeRI GACNNNNNGTC 1 cut(s) 71
BmeT110I CYCGRG 3 cut(s) 401, 673, 810
BmgT120I GGNCC 5 cut(s) 105, 298, 373, 670, 671
BmiI GGNNCC 4 cut(s) 107, 629, 672, 679
BmrFI CCNGG 7 cut(s) 120, 158, 215, 402, 403, 674, 675
BmsI GCATC 7 cut(s) 187, 459, 496, 533, 570, 608, 780
BpmI CTGGAG 1 cut(s) 180
BpuEI CTTGAG 3 cut(s) 244, 302, 622
BpuMI CCSGG 7 cut(s) 120, 158, 215, 402, 403, 674, 675
BsaAI YACGTR 1 cut(s) 46
BsaI GGTCTC 1 cut(s) 62
BsaJI CCNNGG 4 cut(s) 369, 401, 631, 673
BsaWI WCCGGW 2 cut(s) 277, 624
BsaXI ACNNNNNCTCC 4 cut(s) 144, 174, 768, 798
Bsc4I CCNNNNNNNGG 2 cut(s) 402, 624
Bse118I RCCGGY 2 cut(s) 249, 277
Bse1I ACTGG 1 cut(s) 107
BseDI CCNNGG 4 cut(s) 369, 401, 631, 673
BseLI CCNNNNNNNGG 2 cut(s) 402, 624
BseNI ACTGG 1 cut(s) 107
BseSI GKGCMC 1 cut(s) 674
BseXI GCAGC 2 cut(s) 162, 203
BseYI CCCAGC 1 cut(s) 9
BshFI GGCC 5 cut(s) 106, 253, 290, 299, 672
BshNI GGYRCC 2 cut(s) 627, 677
BshTI ACCGGT 1 cut(s) 277
BsiHKAI GWGCWC 1 cut(s) 213
BsiHKCI CYCGRG 3 cut(s) 401, 673, 810
BsiSI CCGG 9 cut(s) 120, 157, 214, 250, 278, 402, 585, 625, 674
BslFI GGGAC 4 cut(s) 682, 735, 828, 837
BslI CCNNNNNNNGG 2 cut(s) 402, 624
BsmAI GTCTC 1 cut(s) 62
BsmFI GGGAC 4 cut(s) 682, 735, 828, 837
BsnI GGCC 5 cut(s) 106, 253, 290, 299, 672
Bso31I GGTCTC 1 cut(s) 62
BsoBI CYCGRG 3 cut(s) 401, 673, 810
Bsp120I GGGCCC 1 cut(s) 670
Bsp1286I GDGCHC 2 cut(s) 213, 674
Bsp1407I TGTACA 1 cut(s) 829
Bsp143I GATC 2 cut(s) 51, 338
BspACI CCGC 3 cut(s) 768, 837, 853
BspANI GGCC 5 cut(s) 106, 253, 290, 299, 672
BspHI TCATGA 1 cut(s) 664
BspLI GGNNCC 4 cut(s) 107, 629, 672, 679
BspPI GGATC 1 cut(s) 346
BspT107I GGYRCC 2 cut(s) 627, 677
BspTNI GGTCTC 1 cut(s) 62
BsrFI RCCGGY 2 cut(s) 249, 277
BsrGI TGTACA 1 cut(s) 829
BsrI ACTGG 1 cut(s) 107
BssAI RCCGGY 2 cut(s) 249, 277
BssECI CCNNGG 4 cut(s) 369, 401, 631, 673
BssMI GATC 2 cut(s) 51, 338
BssT1I CCWWGG 1 cut(s) 369
BstAUI TGTACA 1 cut(s) 829
BstBAI YACGTR 1 cut(s) 46
BstC8I GCNNGC 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 803
BstHHI GCGC 1 cut(s) 802
BstKTI GATC 2 cut(s) 54, 341
BstMAI GTCTC 1 cut(s) 62
BstMBI GATC 2 cut(s) 51, 338
BstMWI GCNNNNNNNGC 2 cut(s) 197, 296
BstNSI RCATGY 1 cut(s) 146
BstSCI CCNGG 7 cut(s) 118, 156, 213, 400, 401, 672, 673
BstSLI GKGCMC 1 cut(s) 674
BstV1I GCAGC 2 cut(s) 162, 203
BsuI GTATCC 1 cut(s) 338
BsuRI GGCC 5 cut(s) 106, 253, 290, 299, 672
BtsIMutI CAGTG 1 cut(s) 128
Cac8I GCNNGC 1 cut(s) 144
CciI TCATGA 1 cut(s) 664
CfoI GCGC 1 cut(s) 802
Cfr10I RCCGGY 2 cut(s) 249, 277
Cfr13I GGNCC 5 cut(s) 105, 298, 373, 670, 671
Cfr9I CCCGGG 2 cut(s) 401, 673
Csp6I GTAC 7 cut(s) 161, 218, 441, 478, 515, 678, 830
CspAI ACCGGT 1 cut(s) 277
CviAII CATG 6 cut(s) 143, 202, 462, 499, 573, 665
CviQI GTAC 7 cut(s) 161, 218, 441, 478, 515, 678, 830
DpnI GATC 2 cut(s) 53, 340
DpnII GATC 2 cut(s) 51, 338
DriI GACNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 1 cut(s) 251
Eam1105I GACNNNNNGTC 1 cut(s) 71
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 369
Eco147I AGGCCT 1 cut(s) 290
Eco24I GRGCYC 2 cut(s) 213, 674
Eco31I GGTCTC 1 cut(s) 62
Eco47I GGWCC 1 cut(s) 373
Eco53kI GAGCTC 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 22
Eco88I CYCGRG 3 cut(s) 401, 673, 810
EcoICRI GAGCTC 1 cut(s) 211
EcoO109I RGGNCCY 2 cut(s) 298, 670
EcoT14I CCWWGG 1 cut(s) 369
EcoT38I GRGCYC 2 cut(s) 213, 674
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 6 cut(s) 146, 205, 465, 502, 576, 668
FalI AAGNNNNNCTT 2 cut(s) 789, 821
FaqI GGGAC 4 cut(s) 682, 735, 828, 837
FatI CATG 6 cut(s) 142, 201, 461, 498, 572, 664
Fnu4HI GCNGC 3 cut(s) 176, 192, 769
FriOI GRGCYC 2 cut(s) 213, 674
Fsp4HI GCNGC 3 cut(s) 176, 192, 769
FspBI CTAG 2 cut(s) 71, 725
GlaI GCGC 1 cut(s) 801
GluI GCNGC 3 cut(s) 176, 192, 769
GsaI CCCAGC 1 cut(s) 13
GsuI CTGGAG 1 cut(s) 180
HaeII RGCGCY 1 cut(s) 803
HaeIII GGCC 5 cut(s) 106, 253, 290, 299, 672
HapII CCGG 9 cut(s) 120, 157, 214, 250, 278, 402, 585, 625, 674
HhaI GCGC 1 cut(s) 802
Hin1II CATG 6 cut(s) 146, 205, 465, 502, 576, 668
Hin6I GCGC 1 cut(s) 800
HinP1I GCGC 1 cut(s) 800
HinfI GANTC 2 cut(s) 153, 695
HpaII CCGG 9 cut(s) 120, 157, 214, 250, 278, 402, 585, 625, 674
HphI GGTGA 3 cut(s) 70, 76, 239
Hpy188I TCNGA 1 cut(s) 183
Hpy188III TCNNGA 3 cut(s) 555, 593, 665
Hpy99I CGWCG 2 cut(s) 140, 513
HpyAV CCTTC 4 cut(s) 554, 591, 665, 710
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 2 cut(s) 178, 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 296
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 6 cut(s) 146, 205, 465, 502, 576, 668
HspAI GCGC 1 cut(s) 800
KpnI GGTACC 1 cut(s) 681
Kzo9I GATC 2 cut(s) 51, 338
LmnI GCTCC 3 cut(s) 199, 216, 359
Lsp1109I GCAGC 2 cut(s) 162, 203
LweI GCATC 7 cut(s) 187, 459, 496, 533, 570, 608, 780
MaeI CTAG 2 cut(s) 71, 725
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 1 cut(s) 111
MalI GATC 2 cut(s) 53, 340
MboI GATC 2 cut(s) 51, 338
MboII GAAGA 1 cut(s) 61
MfeI CAATTG 1 cut(s) 231
MhlI GDGCHC 2 cut(s) 213, 674
MluCI AATT 2 cut(s) 27, 231
MlyI GAGTC 2 cut(s) 162, 704
MseI TTAA 1 cut(s) 780
MslI CAYNNNNRTG 1 cut(s) 625
MspA1I CMGCKG 1 cut(s) 175
MspI CCGG 9 cut(s) 120, 157, 214, 250, 278, 402, 585, 625, 674
MspR9I CCNGG 7 cut(s) 120, 158, 215, 402, 403, 674, 675
MunI CAATTG 1 cut(s) 231
MwoI GCNNNNNNNGC 2 cut(s) 197, 296
NciI CCSGG 7 cut(s) 120, 158, 215, 402, 403, 674, 675
NdeII GATC 2 cut(s) 51, 338
NlaIII CATG 6 cut(s) 146, 205, 465, 502, 576, 668
NlaIV GGNNCC 4 cut(s) 107, 629, 672, 679
NmeAIII GCCGAG 1 cut(s) 656
NmuCI GTSAC 1 cut(s) 111
NspI RCATGY 1 cut(s) 146
PaeI GCATGC 1 cut(s) 146
PagI TCATGA 1 cut(s) 664
PceI AGGCCT 1 cut(s) 290
PinAI ACCGGT 1 cut(s) 277
PkrI GCNGC 3 cut(s) 177, 193, 770
PleI GAGTC 2 cut(s) 161, 703
PpsI GAGTC 2 cut(s) 161, 703
Ppu21I YACGTR 1 cut(s) 46
Psp124BI GAGCTC 1 cut(s) 213
PspFI CCCAGC 1 cut(s) 9
PspN4I GGNNCC 4 cut(s) 107, 629, 672, 679
PspOMI GGGCCC 1 cut(s) 670
PspPI GGNCC 5 cut(s) 105, 298, 373, 670, 671
PvuII CAGCTG 1 cut(s) 175
RsaI GTAC 7 cut(s) 162, 219, 442, 479, 516, 679, 831
RsaNI GTAC 7 cut(s) 161, 218, 441, 478, 515, 678, 830
RseI CAYNNNNRTG 1 cut(s) 625
SacI GAGCTC 1 cut(s) 213
SaqAI TTAA 1 cut(s) 780
SatI GCNGC 3 cut(s) 176, 192, 769
Sau3AI GATC 2 cut(s) 51, 338
Sau96I GGNCC 5 cut(s) 105, 298, 373, 670, 671
SchI GAGTC 2 cut(s) 162, 704
ScrFI CCNGG 7 cut(s) 120, 158, 215, 402, 403, 674, 675
SduI GDGCHC 2 cut(s) 213, 674
SfaNI GCATC 7 cut(s) 187, 459, 496, 533, 570, 608, 780
SfiI GGCCNNNNNGGCC 1 cut(s) 296
SinI GGWCC 1 cut(s) 373
SmaI CCCGGG 2 cut(s) 403, 675
SmiMI CAYNNNNRTG 1 cut(s) 625
SmlI CTYRAG 3 cut(s) 259, 317, 637
SmoI CTYRAG 3 cut(s) 259, 317, 637
SpeI ACTAGT 1 cut(s) 724
SphI GCATGC 1 cut(s) 146
SrfI GCCCGGGC 1 cut(s) 403
Sse9I AATT 2 cut(s) 27, 231
SseBI AGGCCT 1 cut(s) 290
SsiI CCGC 3 cut(s) 768, 837, 853
SspMI CTAG 2 cut(s) 71, 725
SstI GAGCTC 1 cut(s) 213
StuI AGGCCT 1 cut(s) 290
StyD4I CCNGG 7 cut(s) 118, 156, 213, 400, 401, 672, 673
StyI CCWWGG 1 cut(s) 369
TaiI ACGT 1 cut(s) 48
TaqI TCGA 3 cut(s) 64, 554, 592
TaqII GACCGA 2 cut(s) 44, 149
TasI AATT 2 cut(s) 27, 231
TatI WGTACW 1 cut(s) 829
TauI GCSGC 1 cut(s) 771
Tru1I TTAA 1 cut(s) 780
Tru9I TTAA 1 cut(s) 780
TscAI CASTG 1 cut(s) 135
TseFI GTSAC 1 cut(s) 111
TseI GCWGC 2 cut(s) 175, 191
Tsp45I GTSAC 1 cut(s) 111
TspDTI ATGAA 2 cut(s) 218, 653
TspGWI ACGGA 2 cut(s) 182, 726
TspMI CCCGGG 2 cut(s) 401, 673
TspRI CASTG 1 cut(s) 135
VpaK11BI GGWCC 1 cut(s) 373
XapI RAATTY 1 cut(s) 27
XceI RCATGY 1 cut(s) 146
XmaI CCCGGG 2 cut(s) 401, 673
XspI CTAG 2 cut(s) 71, 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.