Rmu_sc0000147.1_g000027

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000147.1
OG0021081
Physical Location & Seq
Forward (+)
98633 .. 99810
1178 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 723 bp
atgagatcggatggtttgatgatacaaacaacaccagttctatgctttcgtcgcaatgtgggtttgatagttgcctcatttatcattgccttcatcttgaactggagaatcacattagtggtcttagccacgtatcctttgatcattagtggtcacatcagcgagaaacttttcatgaaaggctatggtggcaacttgagcacagcatatctgaaagctaacatgctagctggggaagctgtaagtaacatccgcactgttgctgcattttgttctgaggagaaggtcatcgacctatatgctcatgagcttgttgagccttctagacgttcatttactcgtggtcagattgctggcatattctatggtgtatctctgttcttcatattctcatcctatggcctggccttgtggtatggttctgttttgatggaaaaggagcttgctacctttaaatctgtcatgaaatcattctttgttttcattgtaacggcattagcaatgggtgagactttagcacttgccccagatcttttaaaagggaaccaaatggtggcatcagtctttgatgtaacagatcgtagaacagaagtcctaggggacataggagaagaggttacgaaagtggaaggggcaattgagttaaggggtgttcaatttagccacccatcgagaccagacgtagtgcttttcagggacttcaatttgaaagttcgaacttga

Protein Analysis

240

Amino Acids

26.66

Weight (kDa)

8.52

Isoelectric Point (pI)

32.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 13 - 165 2.5e-26 ABC transporter transmembrane region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021081)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g22520
rosa_chinensis RchiOBHm_Chr2g0146401 RchiOBHm_Chr5g0040371
rosa_multiflora Rmu_sc0000147.1_g000027
rosa_samantha Rh7DG261000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 553
AciI CCGC 1 cut(s) 253
AfiI CCNNNNNNNGG 1 cut(s) 553
AgsI TTSAA 4 cut(s) 100, 656, 703, 709
AjnI CCWGG 1 cut(s) 402
AleI CACNNNNGTG 1 cut(s) 116
AluBI AGCT 5 cut(s) 218, 230, 239, 310, 442
AluI AGCT 5 cut(s) 218, 230, 239, 310, 442
Alw21I GWGCWC 1 cut(s) 203
Alw26I GTCTC 2 cut(s) 503, 667
AoxI GGCC 2 cut(s) 400, 405
ApeKI GCWGC 1 cut(s) 263
Asp700I GAANNNNTTC 2 cut(s) 170, 470
AspA2I CCTAGG 1 cut(s) 595
AsuHPI GGTGA 1 cut(s) 518
AsuII TTCGAA 1 cut(s) 715
AsuNHI GCTAGC 1 cut(s) 226
AvrII CCTAGG 1 cut(s) 595
BauI CACGAG 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 203
BbvI GCAGC 1 cut(s) 250
BccI CCATC 3 cut(s) 5, 424, 676
BceAI ACGGC 1 cut(s) 507
BcgI CGANNNNNNTGC 2 cut(s) 281, 315
BciT130I CCWGG 1 cut(s) 404
BciVI GTATCC 1 cut(s) 144
BclI TGATCA 1 cut(s) 141
BcoDI GTCTC 2 cut(s) 503, 667
BfaI CTAG 3 cut(s) 227, 324, 596
BfuI GTATCC 1 cut(s) 144
BglII AGATCT 1 cut(s) 529
BisI GCNGC 1 cut(s) 264
BlnI CCTAGG 1 cut(s) 595
BlsI GCNGC 1 cut(s) 265
Bme1390I CCNGG 1 cut(s) 404
BmiI GGNNCC 1 cut(s) 545
BmrFI CCNGG 1 cut(s) 404
BmsI GCATC 1 cut(s) 566
BmtI GCTAGC 1 cut(s) 230
BpmI CTGGAG 1 cut(s) 124
Bpu14I TTCGAA 1 cut(s) 715
BpuEI CTTGAG 1 cut(s) 217
BsaAI YACGTR 1 cut(s) 132
BsaI GGTCTC 1 cut(s) 667
BsaJI CCNNGG 1 cut(s) 595
BsaXI ACNNNNNCTCC 2 cut(s) 600, 630
Bsc4I CCNNNNNNNGG 1 cut(s) 553
Bse1I ACTGG 2 cut(s) 35, 107
Bse3DI GCAATG 3 cut(s) 61, 84, 507
BseBI CCWGG 1 cut(s) 404
BseDI CCNNGG 1 cut(s) 595
BseGI GGATG 3 cut(s) 16, 249, 392
BseLI CCNNNNNNNGG 1 cut(s) 553
BseMI GCAATG 3 cut(s) 61, 84, 507
BseMII CTCAG 1 cut(s) 267
BseNI ACTGG 2 cut(s) 35, 107
BseRI GAGGAG 1 cut(s) 293
BseXI GCAGC 1 cut(s) 250
BseYI CCCAGC 1 cut(s) 230
BshFI GGCC 2 cut(s) 402, 407
BsiHKAI GWGCWC 1 cut(s) 203
BslFI GGGAC 2 cut(s) 614, 710
BslI CCNNNNNNNGG 1 cut(s) 553
BsmAI GTCTC 2 cut(s) 503, 667
BsmFI GGGAC 2 cut(s) 614, 710
BsnI GGCC 2 cut(s) 402, 407
Bso31I GGTCTC 1 cut(s) 667
Bsp119I TTCGAA 1 cut(s) 715
Bsp1286I GDGCHC 1 cut(s) 203
Bsp143I GATC 4 cut(s) 5, 141, 529, 577
BspACI CCGC 1 cut(s) 253
BspANI GGCC 2 cut(s) 402, 407
BspCNI CTCAG 1 cut(s) 268
BspHI TCATGA 3 cut(s) 174, 304, 462
BspLI GGNNCC 1 cut(s) 545
BspOI GCTAGC 1 cut(s) 230
BspT104I TTCGAA 1 cut(s) 715
BspTNI GGTCTC 1 cut(s) 667
BsrDI GCAATG 3 cut(s) 61, 84, 507
BsrI ACTGG 2 cut(s) 35, 107
BssECI CCNNGG 1 cut(s) 595
BssMI GATC 4 cut(s) 5, 141, 529, 577
BssSI CACGAG 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 595
Bst2BI CACGAG 1 cut(s) 339
Bst2UI CCWGG 1 cut(s) 404
Bst4CI ACNGT 1 cut(s) 259
Bst6I CTCTTC 1 cut(s) 606
BstBAI YACGTR 1 cut(s) 132
BstBI TTCGAA 1 cut(s) 715
BstC8I GCNNGC 3 cut(s) 228, 355, 444
BstDEI CTNAG 2 cut(s) 124, 276
BstF5I GGATG 3 cut(s) 16, 249, 392
BstKTI GATC 4 cut(s) 8, 144, 532, 580
BstMAI GTCTC 2 cut(s) 503, 667
BstMBI GATC 4 cut(s) 5, 141, 529, 577
BstMWI GCNNNNNNNGC 5 cut(s) 51, 189, 198, 236, 316
BstNI CCWGG 1 cut(s) 404
BstNSI RCATGY 1 cut(s) 226
BstSCI CCNGG 1 cut(s) 402
BstV1I GCAGC 1 cut(s) 250
BstX2I RGATCY 1 cut(s) 529
BstYI RGATCY 1 cut(s) 529
BsuI GTATCC 1 cut(s) 144
BsuRI GGCC 2 cut(s) 402, 407
BtsCI GGATG 3 cut(s) 16, 249, 392
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 3 cut(s) 228, 355, 444
CciI TCATGA 3 cut(s) 174, 304, 462
CviAII CATG 4 cut(s) 175, 223, 305, 463
DdeI CTNAG 2 cut(s) 124, 276
DpnI GATC 4 cut(s) 7, 143, 531, 579
DpnII GATC 4 cut(s) 5, 141, 529, 577
DraI TTTAAA 2 cut(s) 454, 537
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
Eco130I CCWWGG 1 cut(s) 595
Eco31I GGTCTC 1 cut(s) 667
EcoRII CCWGG 1 cut(s) 402
EcoT14I CCWWGG 1 cut(s) 595
ErhI CCWWGG 1 cut(s) 595
FaeI CATG 4 cut(s) 178, 226, 308, 466
FaqI GGGAC 2 cut(s) 614, 710
FatI CATG 4 cut(s) 174, 222, 304, 462
FbaI TGATCA 1 cut(s) 141
Fnu4HI GCNGC 1 cut(s) 264
FokI GGATG 3 cut(s) 23, 236, 379
Fsp4HI GCNGC 1 cut(s) 264
FspBI CTAG 3 cut(s) 227, 324, 596
GluI GCNGC 1 cut(s) 264
GsaI CCCAGC 1 cut(s) 234
GsuI CTGGAG 1 cut(s) 124
HaeIII GGCC 2 cut(s) 402, 407
Hin1II CATG 4 cut(s) 178, 226, 308, 466
HinfI GANTC 1 cut(s) 108
HphI GGTGA 1 cut(s) 518
Hpy188I TCNGA 4 cut(s) 10, 213, 277, 348
Hpy188III TCNNGA 6 cut(s) 97, 175, 305, 324, 463, 672
Hpy99I CGWCG 1 cut(s) 54
HpyAV CCTTC 4 cut(s) 100, 277, 330, 623
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4IV ACGT 3 cut(s) 131, 328, 681
HpyCH4V TGCA 1 cut(s) 266
HpyF10VI GCNNNNNNNGC 5 cut(s) 51, 189, 198, 236, 316
HpyF3I CTNAG 2 cut(s) 124, 276
HpySE526I ACGT 3 cut(s) 131, 328, 681
Hsp92II CATG 4 cut(s) 178, 226, 308, 466
Ksp22I TGATCA 1 cut(s) 141
Kzo9I GATC 4 cut(s) 5, 141, 529, 577
LmnI GCTCC 1 cut(s) 439
LpnPI CCDG 9 cut(s) 48, 88, 216, 339, 389, 416, 540, 679, 690
Lsp1109I GCAGC 1 cut(s) 250
LweI GCATC 1 cut(s) 566
MaeI CTAG 3 cut(s) 227, 324, 596
MaeII ACGT 3 cut(s) 131, 328, 681
MaeIII GTNAC 5 cut(s) 152, 245, 487, 571, 616
MalI GATC 4 cut(s) 7, 143, 531, 579
MboI GATC 4 cut(s) 5, 141, 529, 577
MboII GAAGA 2 cut(s) 373, 623
MfeI CAATTG 1 cut(s) 636
MflI RGATCY 1 cut(s) 529
MhlI GDGCHC 1 cut(s) 203
MluCI AATT 3 cut(s) 636, 656, 703
MnlI CCTC 3 cut(s) 85, 271, 607
MroXI GAANNNNTTC 2 cut(s) 170, 470
MseI TTAA 3 cut(s) 453, 536, 644
MslI CAYNNNNRTG 1 cut(s) 116
MspR9I CCNGG 1 cut(s) 404
MunI CAATTG 1 cut(s) 636
MvaI CCWGG 1 cut(s) 404
MwoI GCNNNNNNNGC 5 cut(s) 51, 189, 198, 236, 316
NdeII GATC 4 cut(s) 5, 141, 529, 577
NheI GCTAGC 1 cut(s) 226
NlaIII CATG 4 cut(s) 178, 226, 308, 466
NlaIV GGNNCC 1 cut(s) 545
NmuCI GTSAC 1 cut(s) 152
NspI RCATGY 1 cut(s) 226
NspV TTCGAA 1 cut(s) 715
OliI CACNNNNGTG 1 cut(s) 116
PagI TCATGA 3 cut(s) 174, 304, 462
PdmI GAANNNNTTC 2 cut(s) 170, 470
PfeI GAWTC 1 cut(s) 108
PflMI CCANNNNNTGG 1 cut(s) 553
PkrI GCNGC 1 cut(s) 265
Ppu21I YACGTR 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 402
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 1 cut(s) 402
PspN4I GGNNCC 1 cut(s) 545
PsuI RGATCY 1 cut(s) 529
RseI CAYNNNNRTG 1 cut(s) 116
SaqAI TTAA 3 cut(s) 453, 536, 644
SatI GCNGC 1 cut(s) 264
Sau3AI GATC 4 cut(s) 5, 141, 529, 577
ScrFI CCNGG 1 cut(s) 404
SduI GDGCHC 1 cut(s) 203
SfaNI GCATC 1 cut(s) 566
SfuI TTCGAA 1 cut(s) 715
SmiMI CAYNNNNRTG 1 cut(s) 116
SmlI CTYRAG 1 cut(s) 196
SmoI CTYRAG 1 cut(s) 196
Sse9I AATT 3 cut(s) 636, 656, 703
SsiI CCGC 1 cut(s) 253
SspMI CTAG 3 cut(s) 227, 324, 596
StyD4I CCNGG 1 cut(s) 402
StyI CCWWGG 1 cut(s) 595
TaaI ACNGT 1 cut(s) 259
TaiI ACGT 3 cut(s) 134, 331, 684
TaqI TCGA 3 cut(s) 291, 671, 715
TasI AATT 3 cut(s) 636, 656, 703
TfiI GAWTC 1 cut(s) 108
Tru1I TTAA 3 cut(s) 453, 536, 644
Tru9I TTAA 3 cut(s) 453, 536, 644
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 1 cut(s) 263
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 7 cut(s) 82, 163, 191, 321, 373, 472, 479
TspRI CASTG 1 cut(s) 262
Van91I CCANNNNNTGG 1 cut(s) 553
XbaI TCTAGA 1 cut(s) 323
XceI RCATGY 1 cut(s) 226
XmaJI CCTAGG 1 cut(s) 595
XmnI GAANNNNTTC 2 cut(s) 170, 470
XspI CTAG 3 cut(s) 227, 324, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.