Rmu_sc0000158.1_g000020

exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000158.1
OG0025452
Physical Location & Seq
Reverse (-)
75973 .. 76509
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000158.1_g000020.1.cds

Sequence Viewer

Length: 537 bp
atgaaaactataataaggatcaaaacactaaaatcccctgtttcaatggaagacttaagatctctcttgttaattgctgaagatgagattaatcaatctgtgaagtccatttctttgtctttagaggctgcgatggctgcatttgatgataattccagaggtactttaccttctcattctttgaataatagtttacctaattccgaaatggtttgctcatctactacacctgctgctatcatttcaaacactaatgctcttacaagcaattcttcaaaacctgttactcaatttagttgtgtgcctcaatcaactgttatatctaccacacctggtgttgcttctccttctggatgcactatgcaatacatgcctaattctgagtatatgatctgtgttgctcttgctgcattaaatggctacgtcaataacctttctccttcaccatacatgttcaatggacccccaaatgacaactgttggatcataattcatatacatatttgtcccctaaaacatggaaattacttgttctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03430 map03430
Pfam Domains
Protein Families
XPG_I XPG_N

Protein Analysis

178

Amino Acids

19.18

Weight (kDa)

6.03

Isoelectric Point (pI)

61.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025452)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000158.1_g000020 Rmu_sc0009360.1_g000035 Rmu_ssc0000116.1_g000046

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 238
Acc36I ACCTGC 1 cut(s) 238
AclWI GGATC 2 cut(s) 26, 491
AcuI CTGAAG 1 cut(s) 99
AdeI CACNNNGTG 1 cut(s) 335
AfaI GTAC 1 cut(s) 163
AflII CTTAAG 1 cut(s) 55
AflIII ACRYGT 1 cut(s) 450
AgsI TTSAA 5 cut(s) 45, 184, 246, 276, 457
AjnI CCWGG 1 cut(s) 331
AlwI GGATC 2 cut(s) 26, 491
ApeKI GCWGC 4 cut(s) 128, 137, 233, 407
AseI ATTAAT 1 cut(s) 90
AspS9I GGNCC 1 cut(s) 461
AsuHPI GGTGA 1 cut(s) 435
AvaII GGWCC 1 cut(s) 461
BarI GAAGNNNNNNTAC 2 cut(s) 154, 186
BbsI GAAGAC 1 cut(s) 57
BbvI GCAGC 4 cut(s) 115, 124, 220, 394
BccI CCATC 1 cut(s) 127
BciT130I CCWGG 1 cut(s) 333
BfrI CTTAAG 1 cut(s) 55
BfuAI ACCTGC 1 cut(s) 238
BglII AGATCT 1 cut(s) 59
BisI GCNGC 4 cut(s) 129, 138, 234, 408
BlsI GCNGC 4 cut(s) 130, 139, 235, 409
Bme1390I CCNGG 1 cut(s) 333
Bme18I GGWCC 1 cut(s) 461
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 1 cut(s) 463
BmrFI CCNGG 1 cut(s) 333
BmsI GCATC 1 cut(s) 344
BpiI GAAGAC 1 cut(s) 57
BseBI CCWGG 1 cut(s) 333
BseGI GGATG 1 cut(s) 359
BseMII CTCAG 1 cut(s) 372
BseXI GCAGC 4 cut(s) 115, 124, 220, 394
BslFI GGGAC 1 cut(s) 492
BsmFI GGGAC 1 cut(s) 492
Bsp143I GATC 4 cut(s) 18, 59, 390, 483
BspCNI CTCAG 1 cut(s) 373
BspLI GGNNCC 1 cut(s) 463
BspMI ACCTGC 1 cut(s) 238
BspPI GGATC 2 cut(s) 26, 491
BspTI CTTAAG 1 cut(s) 55
BssMI GATC 4 cut(s) 18, 59, 390, 483
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 2 cut(s) 316, 479
BstAFI CTTAAG 1 cut(s) 55
BstAPI GCANNNNNTGC 1 cut(s) 370
BstDEI CTNAG 1 cut(s) 381
BstF5I GGATG 1 cut(s) 359
BstKTI GATC 4 cut(s) 21, 62, 393, 486
BstMBI GATC 4 cut(s) 18, 59, 390, 483
BstMWI GCNNNNNNNGC 4 cut(s) 134, 137, 370, 407
BstNI CCWGG 1 cut(s) 333
BstNSI RCATGY 2 cut(s) 373, 454
BstSCI CCNGG 1 cut(s) 331
BstV1I GCAGC 4 cut(s) 115, 124, 220, 394
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 1 cut(s) 59
BstYI RGATCY 1 cut(s) 59
BtgZI GCGATG 1 cut(s) 146
BtsCI GGATG 1 cut(s) 359
BveI ACCTGC 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 461
CsiI ACCWGGT 1 cut(s) 331
Csp6I GTAC 1 cut(s) 162
CviAII CATG 3 cut(s) 370, 451, 518
CviJI RGCY 3 cut(s) 128, 137, 420
CviKI_1 RGCY 3 cut(s) 128, 137, 420
CviQI GTAC 1 cut(s) 162
DdeI CTNAG 1 cut(s) 381
DpnI GATC 4 cut(s) 20, 61, 392, 485
DpnII GATC 4 cut(s) 18, 59, 390, 483
DraIII CACNNNGTG 1 cut(s) 335
Eco47I GGWCC 1 cut(s) 461
Eco57I CTGAAG 1 cut(s) 99
EcoRII CCWGG 1 cut(s) 331
FaeI CATG 3 cut(s) 373, 454, 521
FalI AAGNNNNNCTT 2 cut(s) 256, 288
FaqI GGGAC 1 cut(s) 492
FatI CATG 3 cut(s) 369, 450, 517
Fnu4HI GCNGC 4 cut(s) 129, 138, 234, 408
FokI GGATG 1 cut(s) 366
Fsp4HI GCNGC 4 cut(s) 129, 138, 234, 408
GluI GCNGC 4 cut(s) 129, 138, 234, 408
Hin1II CATG 3 cut(s) 373, 454, 521
HphI GGTGA 1 cut(s) 435
Hpy166II GTNNAC 1 cut(s) 194
Hpy188I TCNGA 2 cut(s) 205, 382
Hpy188III TCNNGA 2 cut(s) 156, 351
Hpy8I GTNNAC 1 cut(s) 194
HpyAV CCTTC 3 cut(s) 180, 357, 450
HpyCH4III ACNGT 2 cut(s) 316, 479
HpyCH4IV ACGT 1 cut(s) 423
HpyCH4V TGCA 4 cut(s) 140, 357, 364, 410
HpyF10VI GCNNNNNNNGC 4 cut(s) 134, 137, 370, 407
HpyF3I CTNAG 1 cut(s) 381
HpySE526I ACGT 1 cut(s) 423
Hsp92II CATG 3 cut(s) 373, 454, 521
Kzo9I GATC 4 cut(s) 18, 59, 390, 483
LpnPI CCDG 7 cut(s) 51, 169, 243, 294, 318, 336, 345
Lsp1109I GCAGC 4 cut(s) 115, 124, 220, 394
LweI GCATC 1 cut(s) 344
MabI ACCWGGT 1 cut(s) 331
MaeII ACGT 1 cut(s) 423
MaeIII GTNAC 1 cut(s) 283
MalI GATC 4 cut(s) 20, 61, 392, 485
MboI GATC 4 cut(s) 18, 59, 390, 483
MboII GAAGA 3 cut(s) 62, 92, 264
MflI RGATCY 1 cut(s) 59
MluCI AATT 8 cut(s) 72, 151, 199, 268, 290, 376, 489, 523
MmeI TCCRAC 1 cut(s) 461
MnlI CCTC 3 cut(s) 118, 152, 315
MseI TTAA 4 cut(s) 56, 71, 90, 413
MspCI CTTAAG 1 cut(s) 55
MspR9I CCNGG 1 cut(s) 333
MvaI CCWGG 1 cut(s) 333
MwoI GCNNNNNNNGC 4 cut(s) 134, 137, 370, 407
NdeII GATC 4 cut(s) 18, 59, 390, 483
NlaIII CATG 3 cut(s) 373, 454, 521
NlaIV GGNNCC 1 cut(s) 463
NspI RCATGY 2 cut(s) 373, 454
PaqCI CACCTGC 1 cut(s) 238
PciI ACATGT 1 cut(s) 450
PkrI GCNGC 4 cut(s) 130, 139, 235, 409
PscI ACATGT 1 cut(s) 450
PshBI ATTAAT 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 1 cut(s) 463
PspPI GGNCC 1 cut(s) 461
PsuI RGATCY 1 cut(s) 59
RsaI GTAC 1 cut(s) 163
RsaNI GTAC 1 cut(s) 162
SaqAI TTAA 4 cut(s) 56, 71, 90, 413
SatI GCNGC 4 cut(s) 129, 138, 234, 408
Sau3AI GATC 4 cut(s) 18, 59, 390, 483
Sau96I GGNCC 1 cut(s) 461
ScrFI CCNGG 1 cut(s) 333
SetI ASST 8 cut(s) 163, 172, 199, 232, 283, 334, 426, 435
SexAI ACCWGGT 1 cut(s) 331
SfaNI GCATC 1 cut(s) 344
SinI GGWCC 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 55
SmoI CTYRAG 1 cut(s) 55
Sse9I AATT 8 cut(s) 72, 151, 199, 268, 290, 376, 489, 523
StyD4I CCNGG 1 cut(s) 331
TaaI ACNGT 2 cut(s) 316, 479
TaiI ACGT 1 cut(s) 426
TasI AATT 8 cut(s) 72, 151, 199, 268, 290, 376, 489, 523
Tru1I TTAA 4 cut(s) 56, 71, 90, 413
Tru9I TTAA 4 cut(s) 56, 71, 90, 413
TseI GCWGC 4 cut(s) 128, 137, 233, 407
TspDTI ATGAA 2 cut(s) 17, 482
Vha464I CTTAAG 1 cut(s) 55
VpaK11BI GGWCC 1 cut(s) 461
VspI ATTAAT 1 cut(s) 90
XceI RCATGY 2 cut(s) 373, 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.