Rmu_sc0000318.1_g000048
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000318.1
OG0025477
Physical Location & Seq
Reverse (-)
165423 .. 166031
609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000318.1_g000048.1.cds

Sequence Viewer

Length: 462 bp
atggatgtaaacagtgtccacaatgctgttcttgctggtggatcttctagggggaaggagttgccggggaaggagctttgcaagggaattaatcctgatgaggctatagcatatggtgctgcagttcaagctgcagtcttgagtgggaatggaaatgagaagcttcaaggttttactctcttggatgtcattcctctatcacttggagtggagactaatgattttggtgacccacattttatgtgggttgtgcttccaagaaacaccagaatccccgcgacaagaacggaattattttggaaacttccattgacaaccagtcctgcagcacctgcaggtgtagtttcctttgaacttggctttgatatttatgcaaatggtatcctgactgtctcagccgtggtcaggggcactgcccagaagaaaaagaaaaagattacaatcatcactaataaattgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

153

Amino Acids

16.26

Weight (kDa)

9.1

Isoelectric Point (pI)

19.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025477)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000318.1_g000048 Rmu_sc0006159.1_g000009 Rmu_sc0006159.1_g000021

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 326, 340
Acc36I ACCTGC 2 cut(s) 326, 340
AccII CGCG 1 cut(s) 278
AciI CCGC 1 cut(s) 276
AclWI GGATC 1 cut(s) 49
AfiI CCNNNNNNNGG 1 cut(s) 405
AgsI TTSAA 3 cut(s) 128, 167, 353
AhdI GACNNNNNGTC 1 cut(s) 318
AluBI AGCT 3 cut(s) 76, 131, 163
AluI AGCT 3 cut(s) 76, 131, 163
Alw26I GTCTC 2 cut(s) 206, 397
AlwI GGATC 1 cut(s) 49
AlwNI CAGNNNCTG 1 cut(s) 332
ApeKI GCWGC 3 cut(s) 119, 131, 326
AseI ATTAAT 1 cut(s) 90
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 1 cut(s) 239
BaeGI GKGCMC 1 cut(s) 413
BbvI GCAGC 3 cut(s) 106, 118, 338
BceAI ACGGC 1 cut(s) 383
BciVI GTATCC 1 cut(s) 392
BcnI CCSGG 1 cut(s) 66
BcoDI GTCTC 2 cut(s) 206, 397
BfaI CTAG 1 cut(s) 48
BfmI CTRYAG 5 cut(s) 105, 120, 132, 324, 333
BfuAI ACCTGC 2 cut(s) 326, 340
BfuI GTATCC 1 cut(s) 392
BisI GCNGC 3 cut(s) 120, 132, 327
BlsI GCNGC 3 cut(s) 121, 133, 328
Bme1390I CCNGG 1 cut(s) 66
BmeRI GACNNNNNGTC 1 cut(s) 318
BmrFI CCNGG 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 160
BpuMI CCSGG 1 cut(s) 66
BsaBI GATNNNNATC 1 cut(s) 440
BsaJI CCNNGG 2 cut(s) 65, 399
Bsc4I CCNNNNNNNGG 1 cut(s) 405
Bse1I ACTGG 1 cut(s) 318
Bse8I GATNNNNATC 1 cut(s) 440
BseDI CCNNGG 2 cut(s) 65, 399
BseGI GGATG 2 cut(s) 10, 190
BseJI GATNNNNATC 1 cut(s) 440
BseLI CCNNNNNNNGG 1 cut(s) 405
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 1 cut(s) 318
BseSI GKGCMC 1 cut(s) 413
BseXI GCAGC 3 cut(s) 106, 118, 338
Bsh1236I CGCG 1 cut(s) 278
BsiSI CCGG 1 cut(s) 65
BslI CCNNNNNNNGG 1 cut(s) 405
BsmAI GTCTC 2 cut(s) 206, 397
Bsp1286I GDGCHC 1 cut(s) 413
Bsp143I GATC 1 cut(s) 41
BspACI CCGC 1 cut(s) 276
BspCNI CTCAG 1 cut(s) 407
BspFNI CGCG 1 cut(s) 278
BspMAI CTGCAG 4 cut(s) 124, 136, 328, 337
BspMI ACCTGC 2 cut(s) 326, 340
BspPI GGATC 1 cut(s) 49
BsrI ACTGG 1 cut(s) 318
BssECI CCNNGG 2 cut(s) 65, 399
BssMI GATC 1 cut(s) 41
Bst4CI ACNGT 2 cut(s) 14, 391
BstAPI GCANNNNNTGC 2 cut(s) 116, 332
BstDEI CTNAG 1 cut(s) 394
BstDSI CCRYGG 1 cut(s) 399
BstEII GGTNACC 1 cut(s) 227
BstF5I GGATG 2 cut(s) 10, 190
BstFNI CGCG 1 cut(s) 278
BstKTI GATC 1 cut(s) 44
BstMAI GTCTC 2 cut(s) 206, 397
BstMBI GATC 1 cut(s) 41
BstMWI GCNNNNNNNGC 4 cut(s) 32, 116, 128, 332
BstPI GGTNACC 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 64
BstSFI CTRYAG 5 cut(s) 105, 120, 132, 324, 333
BstSLI GKGCMC 1 cut(s) 413
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 3 cut(s) 106, 118, 338
BstX2I RGATCY 1 cut(s) 41
BstYI RGATCY 1 cut(s) 41
BsuI GTATCC 1 cut(s) 392
BtgI CCRYGG 1 cut(s) 399
BtsCI GGATG 2 cut(s) 10, 190
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 2 cut(s) 19, 411
BveI ACCTGC 2 cut(s) 326, 340
CaiI CAGNNNCTG 1 cut(s) 332
CviJI RGCY 6 cut(s) 76, 104, 131, 163, 360, 398
CviKI_1 RGCY 6 cut(s) 76, 104, 131, 163, 360, 398
DdeI CTNAG 1 cut(s) 394
DpnI GATC 1 cut(s) 43
DpnII GATC 1 cut(s) 41
DriI GACNNNNNGTC 1 cut(s) 318
Eam1105I GACNNNNNGTC 1 cut(s) 318
Eco91I GGTNACC 1 cut(s) 227
EcoO65I GGTNACC 1 cut(s) 227
FaiI YATR 5 cut(s) 107, 112, 114, 242, 372
FauI CCCGC 1 cut(s) 283
FauNDI CATATG 1 cut(s) 112
Fnu4HI GCNGC 3 cut(s) 120, 132, 327
FokI GGATG 2 cut(s) 17, 197
Fsp4HI GCNGC 3 cut(s) 120, 132, 327
FspBI CTAG 1 cut(s) 48
GluI GCNGC 3 cut(s) 120, 132, 327
HapII CCGG 1 cut(s) 65
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 1 cut(s) 270
HpaII CCGG 1 cut(s) 65
HphI GGTGA 1 cut(s) 239
Hpy166II GTNNAC 2 cut(s) 10, 19
Hpy188III TCNNGA 3 cut(s) 95, 139, 385
Hpy8I GTNNAC 2 cut(s) 10, 19
HpyAV CCTTC 2 cut(s) 49, 64
HpyCH4III ACNGT 2 cut(s) 14, 391
HpyCH4V TGCA 6 cut(s) 81, 122, 134, 326, 335, 374
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 116, 128, 332
HpyF3I CTNAG 1 cut(s) 394
Kzo9I GATC 1 cut(s) 41
LmnI GCTCC 1 cut(s) 73
Lsp1109I GCAGC 3 cut(s) 106, 118, 338
MaeI CTAG 1 cut(s) 48
MaeIII GTNAC 1 cut(s) 227
MalI GATC 1 cut(s) 43
MboI GATC 1 cut(s) 41
MboII GAAGA 2 cut(s) 36, 433
MflI RGATCY 1 cut(s) 41
MhlI GDGCHC 1 cut(s) 413
MluCI AATT 3 cut(s) 87, 290, 455
MnlI CCTC 2 cut(s) 94, 204
MseI TTAA 1 cut(s) 90
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 1 cut(s) 66
MvnI CGCG 1 cut(s) 278
MwoI GCNNNNNNNGC 4 cut(s) 32, 116, 128, 332
NciI CCSGG 1 cut(s) 66
NdeI CATATG 1 cut(s) 112
NdeII GATC 1 cut(s) 41
NmuCI GTSAC 1 cut(s) 227
PaqCI CACCTGC 2 cut(s) 326, 340
PfeI GAWTC 1 cut(s) 270
PkrI GCNGC 3 cut(s) 121, 133, 328
PshBI ATTAAT 1 cut(s) 90
PspEI GGTNACC 1 cut(s) 227
PstI CTGCAG 4 cut(s) 124, 136, 328, 337
PstNI CAGNNNCTG 1 cut(s) 332
PsuI RGATCY 1 cut(s) 41
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 3 cut(s) 120, 132, 327
Sau3AI GATC 1 cut(s) 41
SbfI CCTGCAGG 1 cut(s) 337
ScrFI CCNGG 1 cut(s) 66
SdaI CCTGCAGG 1 cut(s) 337
SduI GDGCHC 1 cut(s) 413
SetI ASST 6 cut(s) 78, 133, 165, 172, 334, 340
SfcI CTRYAG 5 cut(s) 105, 120, 132, 324, 333
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
Sse8387I CCTGCAGG 1 cut(s) 337
Sse9I AATT 3 cut(s) 87, 290, 455
SsiI CCGC 1 cut(s) 276
SspMI CTAG 1 cut(s) 48
StyD4I CCNGG 1 cut(s) 64
TaaI ACNGT 2 cut(s) 14, 391
TasI AATT 3 cut(s) 87, 290, 455
TfiI GAWTC 1 cut(s) 270
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TscAI CASTG 2 cut(s) 19, 418
TseFI GTSAC 1 cut(s) 227
TseI GCWGC 3 cut(s) 119, 131, 326
Tsp45I GTSAC 1 cut(s) 227
TspGWI ACGGA 1 cut(s) 302
TspRI CASTG 2 cut(s) 19, 418
VspI ATTAAT 1 cut(s) 90
XcmI CCANNNNNNNNNTGG 1 cut(s) 240
XspI CTAG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.