Rmu_sc0006159.1_g000021
HSP70 Family

Heat shock cognate 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006159.1
OG0025477
Physical Location & Seq
Reverse (-)
80781 .. 81210
430 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006159.1_g000021.1.cds

Sequence Viewer

Length: 369 bp
atggatgtaaacagtgtccacaatgctgttcttgctggtggatcttctagggggaaggagttgccggggaaggagctttgcaagggaattaatcctgatgaggctatagcatatggtgctgcagttcaagctgcagtcttgagtgggaatggaaatgagaagcttcaaggttttactctcttggatgtcattcctctatcacttggagtggagactaatgattttggtgacccacattttatgtgggttgtgcttccaagaaacaccagaatccccgcgacaagaacggaattattttggaaacttccattgacaaccaggtttcagttccattctcaatttatgaggaccagagttcagcaactttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

122

Amino Acids

13.45

Weight (kDa)

7.95

Isoelectric Point (pI)

13.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025477)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000318.1_g000048 Rmu_sc0006159.1_g000009 Rmu_sc0006159.1_g000021

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 278
AciI CCGC 1 cut(s) 276
AclWI GGATC 1 cut(s) 49
AgsI TTSAA 2 cut(s) 128, 167
AjnI CCWGG 1 cut(s) 317
AluBI AGCT 3 cut(s) 76, 131, 163
AluI AGCT 3 cut(s) 76, 131, 163
Alw26I GTCTC 1 cut(s) 206
AlwI GGATC 1 cut(s) 49
ApeKI GCWGC 2 cut(s) 119, 131
AseI ATTAAT 1 cut(s) 90
AspS9I GGNCC 1 cut(s) 348
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 1 cut(s) 239
AvaII GGWCC 1 cut(s) 348
BbvI GCAGC 2 cut(s) 106, 118
BciT130I CCWGG 1 cut(s) 319
BcnI CCSGG 1 cut(s) 66
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 1 cut(s) 48
BfmI CTRYAG 3 cut(s) 105, 120, 132
BisI GCNGC 2 cut(s) 120, 132
BlsI GCNGC 2 cut(s) 121, 133
Bme1390I CCNGG 2 cut(s) 66, 319
Bme18I GGWCC 1 cut(s) 348
BmgT120I GGNCC 1 cut(s) 348
BmrFI CCNGG 2 cut(s) 66, 319
BpuEI CTTGAG 1 cut(s) 160
BpuMI CCSGG 1 cut(s) 66
BsaJI CCNNGG 1 cut(s) 65
BseBI CCWGG 1 cut(s) 319
BseDI CCNNGG 1 cut(s) 65
BseGI GGATG 2 cut(s) 10, 190
BseXI GCAGC 2 cut(s) 106, 118
Bsh1236I CGCG 1 cut(s) 278
BsiSI CCGG 1 cut(s) 65
BsmAI GTCTC 1 cut(s) 206
Bsp143I GATC 1 cut(s) 41
BspACI CCGC 1 cut(s) 276
BspFNI CGCG 1 cut(s) 278
BspMAI CTGCAG 2 cut(s) 124, 136
BspPI GGATC 1 cut(s) 49
BssECI CCNNGG 1 cut(s) 65
BssMI GATC 1 cut(s) 41
Bst2UI CCWGG 1 cut(s) 319
Bst4CI ACNGT 1 cut(s) 14
BstAPI GCANNNNNTGC 1 cut(s) 116
BstEII GGTNACC 1 cut(s) 227
BstF5I GGATG 2 cut(s) 10, 190
BstFNI CGCG 1 cut(s) 278
BstKTI GATC 1 cut(s) 44
BstMAI GTCTC 1 cut(s) 206
BstMBI GATC 1 cut(s) 41
BstMWI GCNNNNNNNGC 3 cut(s) 32, 116, 128
BstNI CCWGG 1 cut(s) 319
BstPI GGTNACC 1 cut(s) 227
BstSCI CCNGG 2 cut(s) 64, 317
BstSFI CTRYAG 3 cut(s) 105, 120, 132
BstUI CGCG 1 cut(s) 278
BstV1I GCAGC 2 cut(s) 106, 118
BstX2I RGATCY 1 cut(s) 41
BstYI RGATCY 1 cut(s) 41
BtsCI GGATG 2 cut(s) 10, 190
BtsIMutI CAGTG 1 cut(s) 19
Cfr13I GGNCC 1 cut(s) 348
CsiI ACCWGGT 1 cut(s) 317
CviJI RGCY 4 cut(s) 76, 104, 131, 163
CviKI_1 RGCY 4 cut(s) 76, 104, 131, 163
DpnI GATC 1 cut(s) 43
DpnII GATC 1 cut(s) 41
Eco47I GGWCC 1 cut(s) 348
Eco91I GGTNACC 1 cut(s) 227
EcoO65I GGTNACC 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 317
FaiI YATR 5 cut(s) 107, 112, 114, 242, 344
FauI CCCGC 1 cut(s) 283
FauNDI CATATG 1 cut(s) 112
Fnu4HI GCNGC 2 cut(s) 120, 132
FokI GGATG 2 cut(s) 17, 197
Fsp4HI GCNGC 2 cut(s) 120, 132
FspBI CTAG 1 cut(s) 48
GluI GCNGC 2 cut(s) 120, 132
HapII CCGG 1 cut(s) 65
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 1 cut(s) 270
HpaII CCGG 1 cut(s) 65
HphI GGTGA 1 cut(s) 239
Hpy166II GTNNAC 2 cut(s) 10, 19
Hpy188III TCNNGA 2 cut(s) 95, 139
Hpy8I GTNNAC 2 cut(s) 10, 19
HpyAV CCTTC 2 cut(s) 49, 64
HpyCH4III ACNGT 1 cut(s) 14
HpyCH4V TGCA 3 cut(s) 81, 122, 134
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 116, 128
Kzo9I GATC 1 cut(s) 41
LmnI GCTCC 1 cut(s) 73
LpnPI CCDG 7 cut(s) 21, 78, 108, 280, 304, 331, 364
Lsp1109I GCAGC 2 cut(s) 106, 118
MabI ACCWGGT 1 cut(s) 317
MaeI CTAG 1 cut(s) 48
MaeIII GTNAC 1 cut(s) 227
MalI GATC 1 cut(s) 43
MboI GATC 1 cut(s) 41
MboII GAAGA 1 cut(s) 36
MflI RGATCY 1 cut(s) 41
MluCI AATT 3 cut(s) 87, 290, 338
MnlI CCTC 3 cut(s) 94, 204, 339
MseI TTAA 1 cut(s) 90
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 2 cut(s) 66, 319
MvaI CCWGG 1 cut(s) 319
MvnI CGCG 1 cut(s) 278
MwoI GCNNNNNNNGC 3 cut(s) 32, 116, 128
NciI CCSGG 1 cut(s) 66
NdeI CATATG 1 cut(s) 112
NdeII GATC 1 cut(s) 41
NmuCI GTSAC 1 cut(s) 227
PfeI GAWTC 1 cut(s) 270
PkrI GCNGC 2 cut(s) 121, 133
PshBI ATTAAT 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 317
PspEI GGTNACC 1 cut(s) 227
PspGI CCWGG 1 cut(s) 317
PspPI GGNCC 1 cut(s) 348
PstI CTGCAG 2 cut(s) 124, 136
PsuI RGATCY 1 cut(s) 41
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 2 cut(s) 120, 132
Sau3AI GATC 1 cut(s) 41
Sau96I GGNCC 1 cut(s) 348
ScrFI CCNGG 2 cut(s) 66, 319
SetI ASST 5 cut(s) 78, 133, 165, 172, 323
SexAI ACCWGGT 1 cut(s) 317
SfcI CTRYAG 3 cut(s) 105, 120, 132
SinI GGWCC 1 cut(s) 348
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
Sse9I AATT 3 cut(s) 87, 290, 338
SsiI CCGC 1 cut(s) 276
SspMI CTAG 1 cut(s) 48
StyD4I CCNGG 2 cut(s) 64, 317
TaaI ACNGT 1 cut(s) 14
TasI AATT 3 cut(s) 87, 290, 338
TfiI GAWTC 1 cut(s) 270
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TscAI CASTG 1 cut(s) 19
TseFI GTSAC 1 cut(s) 227
TseI GCWGC 2 cut(s) 119, 131
Tsp45I GTSAC 1 cut(s) 227
TspGWI ACGGA 1 cut(s) 302
TspRI CASTG 1 cut(s) 19
VpaK11BI GGWCC 1 cut(s) 348
VspI ATTAAT 1 cut(s) 90
XcmI CCANNNNNNNNNTGG 1 cut(s) 240
XspI CTAG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.