Rmu_sc0000659.1_g000018

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000659.1
OG0021637
Physical Location & Seq
Reverse (-)
70369 .. 73435
3067 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 681 bp
atgttacaccgaaagtactggaagagtgtgtggaagtccaatgtttcaccgaaagtgaggagattcatttggaggcttatgcgtggcatagtcccaacacgccagaatcttgttaagcgtgttccattattggtggatctgcagtgtgcattcagtcacaagcacacggaggatgagttgcacttgttcaagcacacggaggtggctcccttatggttgagctgtccgttggcattgcgttcctctgaacacccagctcgttcaattgctgattggattatggccatgatggcatggatactctcaaacccaagccgagggacttctactttttgtgatgatttatcagatgttgggcgcattgtggaggattgtaagtcctactttaatgctgtcagttctatacaattgcgtcacatctatcgtgaagcaaatggtgtggtcaatagcctcttcccaacccagcctatacccacttgctacgaggacttttgggcctttctccaatgggtcgccacccatgtgaaaggaagcaggccggatcaatggctaaacgagtacgccgacttgaaccgcgtcttcgtcagcggagatagcacgggagggaccgacaatgagatgtggctttacataaacaaggagaatggagggttgcaggataggaggccggttcaggtgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0008152 GO:0009056 GO:0016787 GO:0016788 GO:0044424 GO:0044444 GO:0044464 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

226

Amino Acids

26.23

Weight (kDa)

8.83

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 5 - 70 2.4e-10 zinc-binding in reverse transcriptase
Abhydrolase_3 PF07859 151 - 203 8.4e-09 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 576
AciI CCGC 2 cut(s) 574, 588
AclWI GGATC 2 cut(s) 144, 549
AcoI YGGCCR 1 cut(s) 282
AfaI GTAC 2 cut(s) 17, 560
AfiI CCNNNNNNNGG 1 cut(s) 317
AgsI TTSAA 3 cut(s) 190, 264, 571
AleI CACNNNNGTG 2 cut(s) 200, 521
AluBI AGCT 2 cut(s) 222, 257
AluI AGCT 2 cut(s) 222, 257
AlwI GGATC 2 cut(s) 144, 549
AoxI GGCC 4 cut(s) 282, 495, 536, 665
AspLEI GCGC 1 cut(s) 360
AspS9I GGNCC 2 cut(s) 495, 606
AsuHPI GGTGA 1 cut(s) 39
AvaII GGWCC 1 cut(s) 606
BalI TGGCCA 1 cut(s) 284
BbsI GAAGAC 1 cut(s) 571
BccI CCATC 1 cut(s) 283
BciVI GTATCC 1 cut(s) 291
BfmI CTRYAG 1 cut(s) 140
BfuI GTATCC 1 cut(s) 291
BglI GCCNNNNNGGC 1 cut(s) 290
BmcAI AGTACT 1 cut(s) 17
Bme18I GGWCC 1 cut(s) 606
BmgT120I GGNCC 2 cut(s) 495, 606
BmiI GGNNCC 2 cut(s) 207, 607
BpiI GAAGAC 1 cut(s) 571
BsaJI CCNNGG 1 cut(s) 316
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 1 cut(s) 317
Bse118I RCCGGY 1 cut(s) 667
Bse1I ACTGG 1 cut(s) 23
Bse3DI GCAATG 1 cut(s) 233
BseDI CCNNGG 1 cut(s) 316
BseGI GGATG 1 cut(s) 178
BseLI CCNNNNNNNGG 1 cut(s) 317
BseMI GCAATG 1 cut(s) 233
BseNI ACTGG 1 cut(s) 23
BseRI GAGGAG 1 cut(s) 73
BseYI CCCAGC 2 cut(s) 253, 462
Bsh1236I CGCG 1 cut(s) 576
BshFI GGCC 4 cut(s) 284, 497, 538, 667
BsiSI CCGG 2 cut(s) 539, 668
BslFI GGGAC 3 cut(s) 77, 334, 619
BslI CCNNNNNNNGG 1 cut(s) 317
BsmFI GGGAC 3 cut(s) 77, 334, 619
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 4 cut(s) 284, 497, 538, 667
Bsp143I GATC 2 cut(s) 136, 541
BspACI CCGC 2 cut(s) 574, 588
BspANI GGCC 4 cut(s) 284, 497, 538, 667
BspFNI CGCG 1 cut(s) 576
BspLI GGNNCC 2 cut(s) 207, 607
BspMAI CTGCAG 1 cut(s) 144
BspPI GGATC 2 cut(s) 144, 549
BsrDI GCAATG 1 cut(s) 233
BsrFI RCCGGY 1 cut(s) 667
BsrI ACTGG 1 cut(s) 23
BssAI RCCGGY 1 cut(s) 667
BssECI CCNNGG 1 cut(s) 316
BssMI GATC 2 cut(s) 136, 541
Bst6I CTCTTC 2 cut(s) 17, 458
BstC8I GCNNGC 1 cut(s) 536
BstF5I GGATG 1 cut(s) 178
BstFNI CGCG 1 cut(s) 576
BstHHI GCGC 1 cut(s) 360
BstKTI GATC 2 cut(s) 139, 544
BstMBI GATC 2 cut(s) 136, 541
BstMWI GCNNNNNNNGC 2 cut(s) 290, 594
BstSFI CTRYAG 1 cut(s) 140
BstUI CGCG 1 cut(s) 576
BstV2I GAAGAC 1 cut(s) 571
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
BsuI GTATCC 1 cut(s) 291
BsuRI GGCC 4 cut(s) 284, 497, 538, 667
BtsCI GGATG 1 cut(s) 178
BtsI GCAGTG 1 cut(s) 149
BtsIMutI CAGTG 1 cut(s) 149
Cac8I GCNNGC 1 cut(s) 536
CfoI GCGC 1 cut(s) 360
Cfr10I RCCGGY 1 cut(s) 667
Cfr13I GGNCC 2 cut(s) 495, 606
CseI GACGC 2 cut(s) 401, 565
Csp6I GTAC 2 cut(s) 16, 559
CspCI CAANNNNNGTGG 2 cut(s) 420, 455
CviAII CATG 3 cut(s) 286, 294, 521
CviQI GTAC 2 cut(s) 16, 559
DpnI GATC 2 cut(s) 138, 543
DpnII GATC 2 cut(s) 136, 541
EaeI YGGCCR 1 cut(s) 282
Eam1104I CTCTTC 2 cut(s) 17, 458
EarI CTCTTC 2 cut(s) 17, 458
Eco47I GGWCC 1 cut(s) 606
FaeI CATG 3 cut(s) 289, 297, 524
FalI AAGNNNNNCTT 2 cut(s) 368, 400
FaqI GGGAC 3 cut(s) 77, 334, 619
FatI CATG 3 cut(s) 285, 293, 520
FokI GGATG 1 cut(s) 185
GlaI GCGC 1 cut(s) 359
GsaI CCCAGC 2 cut(s) 257, 466
HaeIII GGCC 4 cut(s) 284, 497, 538, 667
HapII CCGG 2 cut(s) 539, 668
HgaI GACGC 2 cut(s) 401, 565
HhaI GCGC 1 cut(s) 360
Hin1II CATG 3 cut(s) 289, 297, 524
Hin6I GCGC 1 cut(s) 358
HinP1I GCGC 1 cut(s) 358
HinfI GANTC 2 cut(s) 63, 106
HpaII CCGG 2 cut(s) 539, 668
HphI GGTGA 1 cut(s) 39
Hpy188I TCNGA 2 cut(s) 247, 349
Hpy188III TCNNGA 1 cut(s) 425
HpyCH4V TGCA 4 cut(s) 142, 149, 181, 655
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 594
Hsp92II CATG 3 cut(s) 289, 297, 524
HspAI GCGC 1 cut(s) 358
Kzo9I GATC 2 cut(s) 136, 541
LmnI GCTCC 1 cut(s) 211
LpnPI CCDG 8 cut(s) 4, 116, 267, 476, 520, 552, 641, 659
MaeIII GTNAC 3 cut(s) 3, 155, 413
MalI GATC 2 cut(s) 138, 543
MboI GATC 2 cut(s) 136, 541
MboII GAAGA 3 cut(s) 34, 445, 571
MfeI CAATTG 2 cut(s) 264, 407
MflI RGATCY 1 cut(s) 136
MlsI TGGCCA 1 cut(s) 284
MluCI AATT 2 cut(s) 264, 407
MluNI TGGCCA 1 cut(s) 284
Mox20I TGGCCA 1 cut(s) 284
MscI TGGCCA 1 cut(s) 284
MseI TTAA 2 cut(s) 114, 387
MslI CAYNNNNRTG 2 cut(s) 200, 521
Msp20I TGGCCA 1 cut(s) 284
MspA1I CMGCKG 1 cut(s) 588
MspI CCGG 2 cut(s) 539, 668
MunI CAATTG 2 cut(s) 264, 407
Mva1269I GAATGC 1 cut(s) 149
MvnI CGCG 1 cut(s) 576
MwoI GCNNNNNNNGC 2 cut(s) 290, 594
NdeII GATC 2 cut(s) 136, 541
NlaIII CATG 3 cut(s) 289, 297, 524
NlaIV GGNNCC 2 cut(s) 207, 607
NmeAIII GCCGAG 1 cut(s) 341
NmuCI GTSAC 2 cut(s) 155, 413
OliI CACNNNNGTG 2 cut(s) 200, 521
PcsI WCGNNNNNNNCGW 1 cut(s) 561
PctI GAATGC 1 cut(s) 149
PfeI GAWTC 2 cut(s) 63, 106
PspFI CCCAGC 2 cut(s) 253, 462
PspN4I GGNNCC 2 cut(s) 207, 607
PspPI GGNCC 2 cut(s) 495, 606
PstI CTGCAG 1 cut(s) 144
PsuI RGATCY 1 cut(s) 136
RsaI GTAC 2 cut(s) 17, 560
RsaNI GTAC 2 cut(s) 16, 559
RseI CAYNNNNRTG 2 cut(s) 200, 521
SaqAI TTAA 2 cut(s) 114, 387
Sau3AI GATC 2 cut(s) 136, 541
Sau96I GGNCC 2 cut(s) 495, 606
ScaI AGTACT 1 cut(s) 17
SetI ASST 4 cut(s) 204, 224, 259, 678
SfcI CTRYAG 1 cut(s) 140
SinI GGWCC 1 cut(s) 606
SmiMI CAYNNNNRTG 2 cut(s) 200, 521
Sse9I AATT 2 cut(s) 264, 407
SsiI CCGC 2 cut(s) 574, 588
TaqII GACCGA 1 cut(s) 623
TasI AATT 2 cut(s) 264, 407
TatI WGTACW 1 cut(s) 15
TfiI GAWTC 2 cut(s) 63, 106
Tru1I TTAA 2 cut(s) 114, 387
Tru9I TTAA 2 cut(s) 114, 387
TscAI CASTG 1 cut(s) 149
TseFI GTSAC 2 cut(s) 155, 413
Tsp45I GTSAC 2 cut(s) 155, 413
TspDTI ATGAA 1 cut(s) 55
TspGWI ACGGA 3 cut(s) 182, 212, 216
TspRI CASTG 1 cut(s) 149
VpaK11BI GGWCC 1 cut(s) 606
ZrmI AGTACT 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.