Rmu_sc0001140.1_g000007

pyridoxal biosynthesis protein PDX1.2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001140.1
OG0024915
Physical Location & Seq
Forward (+)
20731 .. 25462
4732 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 489 bp
atggagggaggaagtacgtgtgctggtgatggcggcggcggcgggaacggcgacaccgacaacagtagtggcaacggcggtggttcttcaatatgtggagtccgattccaccatatcaaaattttggggaaggcggcagggtcgatggtagatggaggtggctcctatgagtacggcggcggtccaggttcctccttgaatcatcttttaattgcttcttgtagctcgaaaaggaaggatgacgccaacgcctactttgtcatcgtcttcgacccaccccacccctacgccagtattctctgcatgttcaatccctccttagtcaaggacatcaaaagtgcctgttcgatccccatcatggccagatctggggtcgggcatttcattgaggctcagatccttgaagccatcaaggtggactacctcaacgagagcgaatatctcgccgtcgccaatgaagaccatcataaacaagcagaaaacgcgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0046982 GO:0046983
KEGG Pathways
Metabolic & Signaling
ko00750 map00750
Pfam Domains
Protein Families
SOR_SNZ

Protein Analysis

162

Amino Acids

16.71

Weight (kDa)

5.36

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOR_SNZ PF01680 99 - 158 4.4e-18 SOR/SNZ family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024915)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0488641
rosa_multiflora Rmu_sc0001140.1_g000007
rosa_roxburghii Rroxscaffold_1G00039440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 485
AciI CCGC 8 cut(s) 33, 36, 39, 42, 78, 134, 177, 180
AclWI GGATC 2 cut(s) 343, 391
AcoI YGGCCR 1 cut(s) 360
AcsI RAATTY 1 cut(s) 120
AcyI GRCGYC 1 cut(s) 243
AfaI GTAC 2 cut(s) 16, 173
AfiI CCNNNNNNNGG 2 cut(s) 358, 369
AflIII ACRYGT 2 cut(s) 17, 483
AgsI TTSAA 4 cut(s) 90, 199, 310, 404
AjnI CCWGG 1 cut(s) 184
AluBI AGCT 1 cut(s) 225
AluI AGCT 1 cut(s) 225
AlwI GGATC 2 cut(s) 343, 391
AoxI GGCC 1 cut(s) 360
ApoI RAATTY 1 cut(s) 120
AspS9I GGNCC 1 cut(s) 182
AsuHPI GGTGA 1 cut(s) 38
AvaII GGWCC 1 cut(s) 182
BalI TGGCCA 1 cut(s) 362
BbsI GAAGAC 2 cut(s) 259, 465
BccI CCATC 6 cut(s) 23, 139, 146, 362, 416, 471
BceAI ACGGC 4 cut(s) 64, 91, 190, 431
BciT130I CCWGG 1 cut(s) 186
BglII AGATCT 1 cut(s) 365
BisI GCNGC 5 cut(s) 34, 37, 40, 135, 178
BlsI GCNGC 5 cut(s) 35, 38, 41, 136, 179
Bme1390I CCNGG 1 cut(s) 186
Bme18I GGWCC 1 cut(s) 182
BmgT120I GGNCC 1 cut(s) 182
BmiI GGNNCC 2 cut(s) 163, 190
BmrFI CCNGG 1 cut(s) 186
BpiI GAAGAC 2 cut(s) 259, 465
BsaAI YACGTR 1 cut(s) 18
BsaBI GATNNNNATC 1 cut(s) 353
BsaHI GRCGYC 1 cut(s) 243
Bsc4I CCNNNNNNNGG 2 cut(s) 358, 369
Bse1I ACTGG 1 cut(s) 291
Bse8I GATNNNNATC 1 cut(s) 353
BseBI CCWGG 1 cut(s) 186
BseGI GGATG 1 cut(s) 244
BseJI GATNNNNATC 1 cut(s) 353
BseLI CCNNNNNNNGG 2 cut(s) 358, 369
BseMII CTCAG 1 cut(s) 407
BseNI ACTGG 1 cut(s) 291
Bsh1236I CGCG 1 cut(s) 485
BshFI GGCC 1 cut(s) 362
BslI CCNNNNNNNGG 2 cut(s) 358, 369
BsnI GGCC 1 cut(s) 362
Bsp143I GATC 3 cut(s) 348, 365, 396
BspACI CCGC 8 cut(s) 33, 36, 39, 42, 78, 134, 177, 180
BspANI GGCC 1 cut(s) 362
BspCNI CTCAG 1 cut(s) 406
BspFNI CGCG 1 cut(s) 485
BspLI GGNNCC 2 cut(s) 163, 190
BspPI GGATC 2 cut(s) 343, 391
BsrI ACTGG 1 cut(s) 291
BssMI GATC 3 cut(s) 348, 365, 396
BssNI GRCGYC 1 cut(s) 243
Bst2UI CCWGG 1 cut(s) 186
Bst4CI ACNGT 1 cut(s) 65
BstACI GRCGYC 1 cut(s) 243
BstBAI YACGTR 1 cut(s) 18
BstDEI CTNAG 2 cut(s) 319, 393
BstF5I GGATG 1 cut(s) 244
BstFNI CGCG 1 cut(s) 485
BstKTI GATC 3 cut(s) 351, 368, 399
BstMBI GATC 3 cut(s) 348, 365, 396
BstMWI GCNNNNNNNGC 3 cut(s) 39, 48, 482
BstNI CCWGG 1 cut(s) 186
BstNSI RCATGY 1 cut(s) 307
BstSCI CCNGG 1 cut(s) 184
BstUI CGCG 1 cut(s) 485
BstV2I GAAGAC 2 cut(s) 259, 465
BstX2I RGATCY 2 cut(s) 365, 396
BstXI CCANNNNNNTGG 1 cut(s) 415
BstYI RGATCY 2 cut(s) 365, 396
BsuRI GGCC 1 cut(s) 362
BtsCI GGATG 1 cut(s) 244
Cfr13I GGNCC 1 cut(s) 182
CseI GACGC 1 cut(s) 251
Csp6I GTAC 2 cut(s) 15, 172
CspCI CAANNNNNGTGG 4 cut(s) 49, 61, 84, 96
CviAII CATG 2 cut(s) 304, 358
CviJI RGCY 5 cut(s) 162, 225, 362, 392, 407
CviKI_1 RGCY 5 cut(s) 162, 225, 362, 392, 407
CviQI GTAC 2 cut(s) 15, 172
DdeI CTNAG 2 cut(s) 319, 393
DpnI GATC 3 cut(s) 350, 367, 398
DpnII GATC 3 cut(s) 348, 365, 396
EaeI YGGCCR 1 cut(s) 360
Eco47I GGWCC 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 184
FaeI CATG 2 cut(s) 307, 361
FaiI YATR 6 cut(s) 94, 114, 168, 305, 359, 468
FatI CATG 2 cut(s) 303, 357
FauI CCCGC 1 cut(s) 35
Fnu4HI GCNGC 5 cut(s) 34, 37, 40, 135, 178
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 5 cut(s) 34, 37, 40, 135, 178
GluI GCNGC 5 cut(s) 34, 37, 40, 135, 178
HaeIII GGCC 1 cut(s) 362
HgaI GACGC 1 cut(s) 251
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 2 cut(s) 307, 361
HinfI GANTC 3 cut(s) 99, 105, 199
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 2 cut(s) 104, 396
Hpy8I GTNNAC 1 cut(s) 418
Hpy99I CGWCG 1 cut(s) 452
HpyAV CCTTC 2 cut(s) 124, 229
HpyCH4III ACNGT 1 cut(s) 65
HpyCH4IV ACGT 1 cut(s) 17
HpyCH4V TGCA 1 cut(s) 303
HpyF10VI GCNNNNNNNGC 3 cut(s) 39, 48, 482
HpyF3I CTNAG 2 cut(s) 319, 393
HpySE526I ACGT 1 cut(s) 17
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 2 cut(s) 307, 361
Kzo9I GATC 3 cut(s) 348, 365, 396
LmnI GCTCC 1 cut(s) 167
LpnPI CCDG 8 cut(s) 9, 123, 171, 198, 304, 354, 355, 376
MaeII ACGT 1 cut(s) 17
MalI GATC 3 cut(s) 350, 367, 398
MboI GATC 3 cut(s) 348, 365, 396
MboII GAAGA 3 cut(s) 78, 259, 470
MflI RGATCY 2 cut(s) 365, 396
MlsI TGGCCA 1 cut(s) 362
MluCI AATT 2 cut(s) 120, 210
MluI ACGCGT 1 cut(s) 483
MluNI TGGCCA 1 cut(s) 362
MlyI GAGTC 1 cut(s) 108
MnlI CCTC 5 cut(s) 149, 202, 325, 382, 434
Mox20I TGGCCA 1 cut(s) 362
MscI TGGCCA 1 cut(s) 362
MseI TTAA 1 cut(s) 209
MslI CAYNNNNRTG 1 cut(s) 413
Msp20I TGGCCA 1 cut(s) 362
MspR9I CCNGG 1 cut(s) 186
MvaI CCWGG 1 cut(s) 186
MvnI CGCG 1 cut(s) 485
MwoI GCNNNNNNNGC 3 cut(s) 39, 48, 482
NdeII GATC 3 cut(s) 348, 365, 396
NlaIII CATG 2 cut(s) 307, 361
NlaIV GGNNCC 2 cut(s) 163, 190
NspI RCATGY 1 cut(s) 307
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PfeI GAWTC 2 cut(s) 105, 199
PkrI GCNGC 5 cut(s) 35, 38, 41, 136, 179
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
Ppu21I YACGTR 1 cut(s) 18
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PspN4I GGNNCC 2 cut(s) 163, 190
PspPI GGNCC 1 cut(s) 182
PsuI RGATCY 2 cut(s) 365, 396
RsaI GTAC 2 cut(s) 16, 173
RsaNI GTAC 2 cut(s) 15, 172
RseI CAYNNNNRTG 1 cut(s) 413
SaqAI TTAA 1 cut(s) 209
SatI GCNGC 5 cut(s) 34, 37, 40, 135, 178
Sau3AI GATC 3 cut(s) 348, 365, 396
Sau96I GGNCC 1 cut(s) 182
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 1 cut(s) 186
SetI ASST 6 cut(s) 20, 160, 190, 227, 417, 426
SinI GGWCC 1 cut(s) 182
SmiMI CAYNNNNRTG 1 cut(s) 413
Sse9I AATT 2 cut(s) 120, 210
SsiI CCGC 8 cut(s) 33, 36, 39, 42, 78, 134, 177, 180
StyD4I CCNGG 1 cut(s) 184
TaaI ACNGT 1 cut(s) 65
TaiI ACGT 1 cut(s) 20
TaqI TCGA 4 cut(s) 143, 227, 270, 347
TasI AATT 2 cut(s) 120, 210
TauI GCSGC 5 cut(s) 36, 39, 42, 137, 180
TfiI GAWTC 2 cut(s) 105, 199
Tru1I TTAA 1 cut(s) 209
Tru9I TTAA 1 cut(s) 209
TspDTI ATGAA 2 cut(s) 373, 471
VpaK11BI GGWCC 1 cut(s) 182
XapI RAATTY 1 cut(s) 120
XceI RCATGY 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.