Rmu_sc0001211.1_g000061

disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001211.1
OG0022652
Physical Location & Seq
Reverse (-)
220498 .. 225720
5223 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3492 bp
atggctgaaacactcgttaatctactcctggagcgtttggcttcggtcaccgttaacaggatcggaagagagctgcagctggtgataggtgttgatgatgaagtcgcaaacctgtcccgcaatttgagacttatcagaaatgtgcttcaggatgcggagagacaagtcacggaggccagcatgggagaagtgacgggggccagcgtgggagattggctgaatgaactgaaccaagtttctcatgagatggacgacgtgttggatgagtggtgcacagaaattcttaagcagcaattactggatgaaaaagaaggtgtgaacccaacggaggccttggagattccagagggttcaaacaaagggggaattattgaaactgctcctcgtactaaggagaaggtatgtctcccccttccttcttgcttttcttgctgccgagtcaaacaagtagttcgtcgtccagtcaatatagtccgtcaagtagttcatcgtcacgacattgcggtcaaaatacgaagtctaaacgaaaagttaactcggattgctaatgaaagacaacgttataactttcaagatacaacagaagcacctaaaccacctccacgacaactaacttcttctcttgtcattgacaaatcctttggtcgaaaaaccgaaaaggaccttttagtaggcaagttgctagatgagagtagtagtcataagagggggggtcctcttatcatccctataataggattgggagggatagggaaaacaactctttgtcaactagtctataatgatgacatggtcaagacctattttgacaagaagatatgggtctgtgtctcagatccttttgaagagattaagattgctaaagccatcatagaaggtcttgataatgtgaacaatactccaaactcaaatcagttagacccttttatggtacgcataaaagaattaatcaagggaaaaaaatttctcctcgtcctagatgatgtgtggaacccaaattctagtcaatggaatgaattaatcaaacccttgcttgagggtgccactggcagtagaatattggtaaccacacgaaaagaggaggttgctcaagtaatgggagcgaccaatcaaatgattcacttgaaggcattaagtgaagagtgcactcgggagctgttcaatcacattaccttccttgatagggaagatgagtttgaaaagtttgaaatgtttgtccaagagtttgtagagaagtgtaaaggtttacctcttgttgcgaagactttaggtagtctcatgcgccataagaaaacaagaaaagaatggcaagatgtattacaaagtccaatatggaaattaaaaaaaattccggaggaggtttttcaaccattattgttaagttattatgatttgtccccaaccataagacgttgtcttttgtattgtgttacttttccgaaagatcatgtgattgataaagaagttttgattgagttgtggatgtcacaagactatttgtaccagaagcaaaagaaagacgaggttggtcaaacatattttgaggagttagcaatgaagtctttcttccaagattttgagagagatgatgatgggaatgttacaaaatgcaaaatgcacgatattatgcatgaccttttgcagttcttgaccaaggaagaatgttttagtatggaggtgaagggtgctgatgagatattggagcttcccaatagtaatagttttcgtcacttcaccctcatgtctgcatctcagggtttatttagcatcctttcttctcagaactgtggaaatttgcgtaccttcactacttttgcttcaacaatttctagtattgagccagagtttataacttcactgaaacgtgttaggaatttaaatttgagtagtactgccctcggagtagttccaaaagaggttggtgaattgctacatttgaggtatcttgatctatcaggtaatgactttactgaattacctgacaaaataggtgatttgtacaatctacaaaccttgcggcttgttgggtgccgtaatctggtaaaagtgcctgatatgaggaagttaatcaatttgaagcatctttacgtcaggggttgtcggcgtttagagtcaaaaggtattagtagattgaaatcgctgcaaacactggatgagtttcacgtgagcgaagataccagagaagaaggatcgcagttggaggctttgggaaacttggacaagcttcaaggggctctttccgtgcaacacttggcatccgcattgcaaactgagtgtggggctgagagagcacggttgaatacgaaacgcctccttcacttggaacttaatttccactgtaactgtagaagaagaatacggaggggcgaagccgaaggagcgatactgaatgctttaaaaccacatccagatttggaatcattaagcatccttgggtgttatggggctaccacgactcccacttggctcatgtctttaccaaacttgaagaggcttactctttacatcaggtatttggaggatttacctgctttagggacactgtcgttccttgaatcgctggatataaagatgactgataaactgcaaaaggtgggtgttgagtttctggggataaatcgaacatcatcatcgtcgtcatcggaggttatttcattcccaaagttaaaacacctcacattcagtttcatggatgaatggaaagagtgggaagaagtggattctcagaatatcaggataatgccatgcctttcatccttagaagtgaggaactgtcggaaactaaaagctctgccggagttcctatggaagacaccactgaagagtttgaccattgcgagttgtccaattctgcattctgtgtactacggacgcgagcgttgtcattggaccacttatggtgaggagccagccccgactaatgaagggtttaacaagatatctcacatcccaatcatcataattgacttccacctcctctgtagaagttgtgtgggtggaaaacccttctggacagctctcattacaatatcatatactaacatccatgggatgcatatctgttatcttaagcatgagagtctctctccatcccaatcatttaagacttgttccatcaaggagtgcgaagaatgcaaatggaatgtcagtttcatgagagaaagcttgcaagggattttccttgacccaaggattgtagacagccccaatctcatggcttcagcacatgaacaactataccgtctacattgttggatttatttcatcaaaacaaagattgaaagcacacctctgtgcggggactggggagtccctgtgaaatcctctgccaacattaaaaggatcaaagatctaatgctgcttctgatgctggactccagctccctatccagtggagatgtctttactctgttacctctatttcttcaagatttggagttgcttgcaagcaaactcttatgtggagggaattgggaggtcaggtctttttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1163

Amino Acids

132.73

Weight (kDa)

6.58

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 6 - 101 7.4e-16 Rx N-terminal domain
NB-ARC PF00931 220 - 392 7.4e-34 NB-ARC domain
WHD_DRP PF23559 479 - 546 1.8e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 599 - 923 7.1e-33 Leucine-rich repeat region
LRR_8 PF13855 622 - 678 2.6e-06 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 733 - 857 5e-20 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022652)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0142201
rosa_multiflora Rmu_sc0001211.1_g000061
rosa_roxburghii Rroxscaffold_2G00103610
rosa_rugosa Rorug02G0371100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 564, 1850
AasI GACNNNNNNGTC 2 cut(s) 503, 3308
Acc36I ACCTGC 1 cut(s) 2536
AccB1I GGYRCC 2 cut(s) 1040, 2028
AccI GTMKAC 2 cut(s) 3198, 3244
AccII CGCG 1 cut(s) 2874
AccIII TCCGGA 1 cut(s) 1351
AciI CCGC 6 cut(s) 118, 155, 503, 2017, 2259, 3297
AclI AACGTT 1 cut(s) 559
AclWI GGATC 4 cut(s) 68, 830, 2197, 3350
AcsI RAATTY 7 cut(s) 279, 962, 997, 1347, 1792, 1873, 1879
AcuI CTGAAG 3 cut(s) 131, 2840, 3204
AcvI CACGTG 1 cut(s) 2164
AfaI GTAC 7 cut(s) 388, 933, 1502, 1801, 1891, 2000, 2864
AfiI CCNNNNNNNGG 9 cut(s) 57, 328, 734, 928, 1183, 2038, 2320, 2534, 3296
AflII CTTAAG 2 cut(s) 284, 3068
AflIII ACRYGT 2 cut(s) 255, 1864
AhlI ACTAGT 1 cut(s) 772
AjiI CACGTC 1 cut(s) 256
AjnI CCWGG 1 cut(s) 27
AjuI GAANNNNNNNTTGG 2 cut(s) 2839, 2871
AleI CACNNNNGTG 1 cut(s) 3292
AloI GAACNNNNNNTCC 2 cut(s) 2316, 2348
AluBI AGCT 9 cut(s) 73, 79, 1156, 1705, 2224, 2789, 3017, 3165, 3381
AluI AGCT 9 cut(s) 73, 79, 1156, 1705, 2224, 2789, 3017, 3165, 3381
Alw21I GWGCWC 3 cut(s) 275, 1148, 2293
Alw26I GTCTC 6 cut(s) 121, 154, 410, 835, 1280, 3086
Alw44I GTGCAC 2 cut(s) 271, 1144
AlwI GGATC 4 cut(s) 68, 830, 2197, 3350
Ama87I CYCGRG 1 cut(s) 1149
Aor13HI TCCGGA 1 cut(s) 1351
AoxI GGCC 3 cut(s) 174, 198, 330
ApaLI GTGCAC 2 cut(s) 271, 1144
ApeKI GCWGC 6 cut(s) 73, 76, 289, 432, 2140, 3358
ApoI RAATTY 7 cut(s) 279, 962, 997, 1347, 1792, 1873, 1879
ArsI GACNNNNNNTTYG 4 cut(s) 623, 655, 3129, 3161
AseI ATTAAT 2 cut(s) 947, 1019
Asp700I GAANNNNTTC 1 cut(s) 1562
AspLEI GCGC 1 cut(s) 1284
AspS9I GGNCC 4 cut(s) 198, 661, 713, 2889
AsuHPI GGTGA 7 cut(s) 40, 94, 1690, 1726, 1934, 2003, 2912
AvaI CYCGRG 1 cut(s) 1149
AvaII GGWCC 3 cut(s) 661, 713, 2889
BaeGI GKGCMC 2 cut(s) 275, 1148
BanI GGYRCC 2 cut(s) 1040, 2028
BanII GRGCYC 1 cut(s) 2236
BarI GAAGNNNNNNTAC 4 cut(s) 2069, 2101, 2367, 2399
BbrPI CACGTG 1 cut(s) 2164
BbsI GAAGAC 2 cut(s) 1268, 2816
Bbv12I GWGCWC 3 cut(s) 275, 1148, 2293
BbvI GCAGC 6 cut(s) 60, 88, 301, 419, 2127, 3345
BccI CCATC 5 cut(s) 241, 875, 1586, 3097, 3122
BceAI ACGGC 1 cut(s) 2016
BciT130I CCWGG 1 cut(s) 29
BcoDI GTCTC 6 cut(s) 121, 154, 410, 835, 1280, 3086
BcuI ACTAGT 1 cut(s) 772
BfaI CTAG 5 cut(s) 683, 773, 977, 1002, 1830
BfmI CTRYAG 3 cut(s) 74, 2344, 2980
BfrI CTTAAG 2 cut(s) 284, 3068
BfuAI ACCTGC 1 cut(s) 2536
BglII AGATCT 1 cut(s) 3349
BisI GCNGC 7 cut(s) 74, 77, 290, 433, 2018, 2141, 3359
BlsI GCNGC 7 cut(s) 75, 78, 291, 434, 2019, 2142, 3360
BmcAI AGTACT 1 cut(s) 1891
Bme1390I CCNGG 1 cut(s) 29
Bme18I GGWCC 3 cut(s) 661, 713, 2889
BmeT110I CYCGRG 1 cut(s) 1149
BmgBI CACGTC 1 cut(s) 256
BmgT120I GGNCC 4 cut(s) 198, 661, 713, 2889
BmiI GGNNCC 6 cut(s) 199, 714, 992, 1042, 2030, 2907
BmrFI CCNGG 1 cut(s) 29
BmrI ACTGGG 1 cut(s) 3313
BmsI GCATC 8 cut(s) 142, 1757, 1776, 2089, 2264, 2436, 3042, 3357
BmuI ACTGGG 1 cut(s) 3313
BpiI GAAGAC 2 cut(s) 1268, 2816
BplI GAGNNNNNCTC 6 cut(s) 2482, 2514, 3068, 3100, 3070, 3102
BpmI CTGGAG 2 cut(s) 50, 3361
BpuEI CTTGAG 2 cut(s) 1055, 1074
BsaAI YACGTR 1 cut(s) 2164
BsaBI GATNNNNATC 2 cut(s) 2134, 3056
BsaJI CCNNGG 6 cut(s) 333, 1653, 1897, 2431, 3046, 3188
BsaWI WCCGGW 1 cut(s) 1351
BsaXI ACNNNNNCTCC 7 cut(s) 2440, 2470, 3300, 3330, 3365, 3395, 3467
Bsc4I CCNNNNNNNGG 9 cut(s) 57, 328, 734, 928, 1183, 2038, 2320, 2534, 3296
Bse1I ACTGG 6 cut(s) 303, 461, 1051, 2154, 3308, 3390
Bse3DI GCAATG 4 cut(s) 498, 1560, 2261, 2832
Bse8I GATNNNNATC 2 cut(s) 2134, 3056
BseAI TCCGGA 1 cut(s) 1351
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 6 cut(s) 333, 1653, 1897, 2431, 3046, 3188
BseJI GATNNNNATC 2 cut(s) 2134, 3056
BseLI CCNNNNNNNGG 9 cut(s) 57, 328, 734, 928, 1183, 2038, 2320, 2534, 3296
BseMI GCAATG 4 cut(s) 498, 1560, 2261, 2832
BseMII CTCAG 6 cut(s) 846, 1766, 1793, 2262, 2274, 2738
BseNI ACTGG 6 cut(s) 303, 461, 1051, 2154, 3308, 3390
BseRI GAGGAG 7 cut(s) 372, 959, 1094, 1370, 1559, 2918, 2966
BseSI GKGCMC 2 cut(s) 275, 1148
BseXI GCAGC 6 cut(s) 60, 88, 301, 419, 2127, 3345
Bsh1236I CGCG 1 cut(s) 2874
BshFI GGCC 3 cut(s) 176, 200, 332
BshNI GGYRCC 2 cut(s) 1040, 2028
BsiHKAI GWGCWC 3 cut(s) 275, 1148, 2293
BsiHKCI CYCGRG 1 cut(s) 1149
BsiSI CCGG 2 cut(s) 1352, 2795
BslFI GGGAC 5 cut(s) 100, 1381, 2551, 3296, 3314
BslI CCNNNNNNNGG 9 cut(s) 57, 328, 734, 928, 1183, 2038, 2320, 2534, 3296
BsmAI GTCTC 6 cut(s) 121, 154, 410, 835, 1280, 3086
BsmFI GGGAC 5 cut(s) 100, 1381, 2551, 3296, 3314
BsmI GAATGC 3 cut(s) 2395, 2854, 3137
BsnI GGCC 3 cut(s) 176, 200, 332
BsoBI CYCGRG 1 cut(s) 1149
Bsp1286I GDGCHC 4 cut(s) 275, 1148, 2236, 2293
Bsp13I TCCGGA 1 cut(s) 1351
Bsp1407I TGTACA 1 cut(s) 1998
Bsp143I GATC 7 cut(s) 60, 835, 1444, 1948, 2189, 3342, 3349
Bsp19I CCATGG 1 cut(s) 3046
BspACI CCGC 6 cut(s) 118, 155, 503, 2017, 2259, 3297
BspANI GGCC 3 cut(s) 176, 200, 332
BspCNI CTCAG 6 cut(s) 845, 1765, 1792, 2263, 2275, 2737
BspEI TCCGGA 1 cut(s) 1351
BspFNI CGCG 1 cut(s) 2874
BspHI TCATGA 2 cut(s) 241, 3153
BspLI GGNNCC 6 cut(s) 199, 714, 992, 1042, 2030, 2907
BspMAI CTGCAG 1 cut(s) 78
BspMI ACCTGC 1 cut(s) 2536
BspPI GGATC 4 cut(s) 68, 830, 2197, 3350
BspT107I GGYRCC 2 cut(s) 1040, 2028
BspTI CTTAAG 2 cut(s) 284, 3068
BsrDI GCAATG 4 cut(s) 498, 1560, 2261, 2832
BsrGI TGTACA 1 cut(s) 1998
BsrI ACTGG 6 cut(s) 303, 461, 1051, 2154, 3308, 3390
BssECI CCNNGG 6 cut(s) 333, 1653, 1897, 2431, 3046, 3188
BssMI GATC 7 cut(s) 60, 835, 1444, 1948, 2189, 3342, 3349
BssT1I CCWWGG 5 cut(s) 333, 1653, 2431, 3046, 3188
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 8 cut(s) 52, 1787, 2295, 2339, 2345, 2544, 2774, 3242
Bst6I CTCTTC 5 cut(s) 61, 840, 1134, 2483, 2816
BstAFI CTTAAG 2 cut(s) 284, 3068
BstAUI TGTACA 1 cut(s) 1998
BstBAI YACGTR 1 cut(s) 2164
BstC8I GCNNGC 7 cut(s) 178, 202, 2876, 2910, 3167, 3444, 3448
BstDEI CTNAG 8 cut(s) 390, 832, 1752, 1779, 2271, 2283, 2724, 2758
BstDSI CCRYGG 1 cut(s) 3046
BstEII GGTNACC 2 cut(s) 46, 1063
BstENI CCTNNNNNAGG 2 cut(s) 732, 2532
BstFNI CGCG 1 cut(s) 2874
BstHHI GCGC 1 cut(s) 1284
BstKTI GATC 7 cut(s) 63, 838, 1447, 1951, 2192, 3345, 3352
BstMAI GTCTC 6 cut(s) 121, 154, 410, 835, 1280, 3086
BstMBI GATC 7 cut(s) 60, 835, 1444, 1948, 2189, 3342, 3349
BstMWI GCNNNNNNNGC 5 cut(s) 429, 2288, 2378, 3132, 3367
BstNI CCWGG 1 cut(s) 29
BstPI GGTNACC 2 cut(s) 46, 1063
BstSCI CCNGG 1 cut(s) 27
BstSFI CTRYAG 3 cut(s) 74, 2344, 2980
BstSLI GKGCMC 2 cut(s) 275, 1148
BstUI CGCG 1 cut(s) 2874
BstV1I GCAGC 6 cut(s) 60, 88, 301, 419, 2127, 3345
BstV2I GAAGAC 2 cut(s) 1268, 2816
BstX2I RGATCY 2 cut(s) 835, 3349
BstXI CCANNNNNNTGG 1 cut(s) 3214
BstYI RGATCY 2 cut(s) 835, 3349
BsuRI GGCC 3 cut(s) 176, 200, 332
BtgI CCRYGG 1 cut(s) 3046
BtrI CACGTC 1 cut(s) 256
BtsIMutI CAGTG 7 cut(s) 1044, 1856, 2147, 2335, 2540, 2816, 3397
BveI ACCTGC 1 cut(s) 2536
Cac8I GCNNGC 7 cut(s) 178, 202, 2876, 2910, 3167, 3444, 3448
CciI TCATGA 2 cut(s) 241, 3153
CfoI GCGC 1 cut(s) 1284
Cfr13I GGNCC 4 cut(s) 198, 661, 713, 2889
CseI GACGC 1 cut(s) 2880
Csp6I GTAC 7 cut(s) 387, 932, 1501, 1800, 1890, 1999, 2863
CviQI GTAC 7 cut(s) 387, 932, 1501, 1800, 1890, 1999, 2863
DdeI CTNAG 8 cut(s) 390, 832, 1752, 1779, 2271, 2283, 2724, 2758
DpnI GATC 7 cut(s) 62, 837, 1446, 1950, 2191, 3344, 3351
DpnII GATC 7 cut(s) 60, 835, 1444, 1948, 2189, 3342, 3349
DraI TTTAAA 2 cut(s) 1878, 2397
DrdI GACNNNNNNGTC 2 cut(s) 503, 3308
DseDI GACNNNNNNGTC 2 cut(s) 503, 3308
Eam1104I CTCTTC 5 cut(s) 61, 840, 1134, 2483, 2816
EarI CTCTTC 5 cut(s) 61, 840, 1134, 2483, 2816
Eco130I CCWWGG 5 cut(s) 333, 1653, 2431, 3046, 3188
Eco147I AGGCCT 1 cut(s) 332
Eco24I GRGCYC 1 cut(s) 2236
Eco32I GATATC 1 cut(s) 2940
Eco47I GGWCC 3 cut(s) 661, 713, 2889
Eco57I CTGAAG 3 cut(s) 131, 2840, 3204
Eco72I CACGTG 1 cut(s) 2164
Eco88I CYCGRG 1 cut(s) 1149
Eco91I GGTNACC 2 cut(s) 46, 1063
EcoNI CCTNNNNNAGG 2 cut(s) 732, 2532
EcoO109I RGGNCCY 2 cut(s) 661, 713
EcoO65I GGTNACC 2 cut(s) 46, 1063
EcoRII CCWGG 1 cut(s) 27
EcoRV GATATC 1 cut(s) 2940
EcoT14I CCWWGG 5 cut(s) 333, 1653, 2431, 3046, 3188
EcoT22I ATGCAT 2 cut(s) 1632, 3057
EcoT38I GRGCYC 1 cut(s) 2236
ErhI CCWWGG 5 cut(s) 333, 1653, 2431, 3046, 3188
FalI AAGNNNNNCTT 4 cut(s) 1550, 1582, 2220, 2252
FaqI GGGAC 5 cut(s) 100, 1381, 2551, 3296, 3314
FauI CCCGC 2 cut(s) 125, 3290
FblI GTMKAC 2 cut(s) 3198, 3244
Fnu4HI GCNGC 7 cut(s) 74, 77, 290, 433, 2018, 2141, 3359
FriOI GRGCYC 1 cut(s) 2236
Fsp4HI GCNGC 7 cut(s) 74, 77, 290, 433, 2018, 2141, 3359
FspBI CTAG 5 cut(s) 683, 773, 977, 1002, 1830
GlaI GCGC 1 cut(s) 1283
GluI GCNGC 7 cut(s) 74, 77, 290, 433, 2018, 2141, 3359
GsuI CTGGAG 2 cut(s) 50, 3361
HaeIII GGCC 3 cut(s) 176, 200, 332
HapII CCGG 2 cut(s) 1352, 2795
HgaI GACGC 1 cut(s) 2880
HhaI GCGC 1 cut(s) 1284
Hin6I GCGC 1 cut(s) 1282
HinP1I GCGC 1 cut(s) 1282
HincII GTYRAC 3 cut(s) 55, 534, 770
HindII GTYRAC 3 cut(s) 55, 534, 770
HindIII AAGCTT 2 cut(s) 2222, 3163
HpaI GTTAAC 2 cut(s) 55, 534
HpaII CCGG 2 cut(s) 1352, 2795
HphI GGTGA 7 cut(s) 40, 94, 1690, 1726, 1934, 2003, 2912
Hpy99I CGWCG 3 cut(s) 257, 459, 2638
HpyCH4III ACNGT 8 cut(s) 52, 1787, 2295, 2339, 2345, 2544, 2774, 3242
HpyCH4IV ACGT 6 cut(s) 255, 559, 1411, 1864, 2088, 2163
HpyF10VI GCNNNNNNNGC 5 cut(s) 429, 2288, 2378, 3132, 3367
HpyF3I CTNAG 8 cut(s) 390, 832, 1752, 1779, 2271, 2283, 2724, 2758
HpySE526I ACGT 6 cut(s) 255, 559, 1411, 1864, 2088, 2163
HspAI GCGC 1 cut(s) 1282
Kpn2I TCCGGA 1 cut(s) 1351
KspAI GTTAAC 2 cut(s) 55, 534
Kzo9I GATC 7 cut(s) 60, 835, 1444, 1948, 2189, 3342, 3349
LmnI GCTCC 8 cut(s) 31, 385, 1100, 1153, 1702, 2378, 2905, 3386
Lsp1109I GCAGC 6 cut(s) 60, 88, 301, 419, 2127, 3345
LweI GCATC 8 cut(s) 142, 1757, 1776, 2089, 2264, 2436, 3042, 3357
MaeI CTAG 5 cut(s) 683, 773, 977, 1002, 1830
MaeII ACGT 6 cut(s) 255, 559, 1411, 1864, 2088, 2163
MalI GATC 7 cut(s) 62, 837, 1446, 1950, 2191, 3344, 3351
MboI GATC 7 cut(s) 60, 835, 1444, 1948, 2189, 3342, 3349
MflI RGATCY 2 cut(s) 835, 3349
MhlI GDGCHC 4 cut(s) 275, 1148, 2236, 2293
MlyI GAGTC 6 cut(s) 447, 2120, 2449, 3088, 3318, 3368
MmeI TCCRAC 4 cut(s) 240, 2178, 2756, 3233
Mph1103I ATGCAT 2 cut(s) 1632, 3057
MroI TCCGGA 1 cut(s) 1351
MroXI GAANNNNTTC 1 cut(s) 1562
MslI CAYNNNNRTG 2 cut(s) 1739, 3292
MspA1I CMGCKG 1 cut(s) 79
MspCI CTTAAG 2 cut(s) 284, 3068
MspI CCGG 2 cut(s) 1352, 2795
MspR9I CCNGG 1 cut(s) 29
Mva1269I GAATGC 3 cut(s) 2395, 2854, 3137
MvaI CCWGG 1 cut(s) 29
MvnI CGCG 1 cut(s) 2874
MwoI GCNNNNNNNGC 5 cut(s) 429, 2288, 2378, 3132, 3367
NcoI CCATGG 1 cut(s) 3046
NdeII GATC 7 cut(s) 60, 835, 1444, 1948, 2189, 3342, 3349
NlaIV GGNNCC 6 cut(s) 199, 714, 992, 1042, 2030, 2907
NmeAIII GCCGAG 1 cut(s) 461
NmuCI GTSAC 6 cut(s) 46, 166, 190, 491, 1485, 1727
NsiI ATGCAT 2 cut(s) 1632, 3057
OliI CACNNNNGTG 1 cut(s) 3292
PagI TCATGA 2 cut(s) 241, 3153
PceI AGGCCT 1 cut(s) 332
PctI GAATGC 3 cut(s) 2395, 2854, 3137
PdmI GAANNNNTTC 1 cut(s) 1562
PfeI GAWTC 5 cut(s) 340, 1117, 2417, 2555, 2720
PflFI GACNNNGTC 3 cut(s) 791, 1413, 2542
PfoI TCCNGGA 1 cut(s) 27
PkrI GCNGC 7 cut(s) 75, 78, 291, 434, 2019, 2142, 3360
PleI GAGTC 6 cut(s) 446, 2119, 2449, 3087, 3317, 3368
PmaCI CACGTG 1 cut(s) 2164
PmlI CACGTG 1 cut(s) 2164
PpsI GAGTC 6 cut(s) 446, 2119, 2449, 3087, 3317, 3368
Ppu21I YACGTR 1 cut(s) 2164
PpuMI RGGWCCY 2 cut(s) 661, 713
PshBI ATTAAT 2 cut(s) 947, 1019
PsiI TTATAA 2 cut(s) 564, 1850
Psp1406I AACGTT 1 cut(s) 559
Psp5II RGGWCCY 2 cut(s) 661, 713
Psp6I CCWGG 1 cut(s) 27
PspCI CACGTG 1 cut(s) 2164
PspEI GGTNACC 2 cut(s) 46, 1063
PspGI CCWGG 1 cut(s) 27
PspN4I GGNNCC 6 cut(s) 199, 714, 992, 1042, 2030, 2907
PspPI GGNCC 4 cut(s) 198, 661, 713, 2889
PspPPI RGGWCCY 2 cut(s) 661, 713
PstI CTGCAG 1 cut(s) 78
PsuI RGATCY 2 cut(s) 835, 3349
PsyI GACNNNGTC 3 cut(s) 791, 1413, 2542
PvuII CAGCTG 1 cut(s) 79
RsaI GTAC 7 cut(s) 388, 933, 1502, 1801, 1891, 2000, 2864
RsaNI GTAC 7 cut(s) 387, 932, 1501, 1800, 1890, 1999, 2863
RseI CAYNNNNRTG 2 cut(s) 1739, 3292
SatI GCNGC 7 cut(s) 74, 77, 290, 433, 2018, 2141, 3359
Sau3AI GATC 7 cut(s) 60, 835, 1444, 1948, 2189, 3342, 3349
Sau96I GGNCC 4 cut(s) 198, 661, 713, 2889
ScaI AGTACT 1 cut(s) 1891
SchI GAGTC 6 cut(s) 447, 2120, 2449, 3088, 3318, 3368
ScrFI CCNGG 1 cut(s) 29
SduI GDGCHC 4 cut(s) 275, 1148, 2236, 2293
SfaNI GCATC 8 cut(s) 142, 1757, 1776, 2089, 2264, 2436, 3042, 3357
SfcI CTRYAG 3 cut(s) 74, 2344, 2980
SinI GGWCC 3 cut(s) 661, 713, 2889
SmiI ATTTAAAT 1 cut(s) 1878
SmiMI CAYNNNNRTG 2 cut(s) 1739, 3292
SmlI CTYRAG 4 cut(s) 284, 1034, 1089, 3068
SmoI CTYRAG 4 cut(s) 284, 1034, 1089, 3068
SpeI ACTAGT 1 cut(s) 772
SseBI AGGCCT 1 cut(s) 332
SsiI CCGC 6 cut(s) 118, 155, 503, 2017, 2259, 3297
SspI AATATT 1 cut(s) 1059
SspMI CTAG 5 cut(s) 683, 773, 977, 1002, 1830
StuI AGGCCT 1 cut(s) 332
StyD4I CCNGG 1 cut(s) 27
StyI CCWWGG 5 cut(s) 333, 1653, 2431, 3046, 3188
SwaI ATTTAAAT 1 cut(s) 1878
TaaI ACNGT 8 cut(s) 52, 1787, 2295, 2339, 2345, 2544, 2774, 3242
TaiI ACGT 6 cut(s) 258, 562, 1414, 1867, 2091, 2166
TaqI TCGA 2 cut(s) 646, 2620
TaqII GACCGA 1 cut(s) 34
TatI WGTACW 3 cut(s) 1889, 1998, 2862
TauI GCSGC 1 cut(s) 2020
TfiI GAWTC 5 cut(s) 340, 1117, 2417, 2555, 2720
TscAI CASTG 7 cut(s) 1051, 1863, 2154, 2342, 2547, 2823, 3397
TseFI GTSAC 6 cut(s) 46, 166, 190, 491, 1485, 1727
TseI GCWGC 6 cut(s) 73, 76, 289, 432, 2140, 3358
Tsp45I GTSAC 6 cut(s) 46, 166, 190, 491, 1485, 1727
TspGWI ACGGA 6 cut(s) 185, 341, 464, 2230, 2374, 2883
TspRI CASTG 7 cut(s) 1051, 1863, 2154, 2342, 2547, 2823, 3397
Tth111I GACNNNGTC 3 cut(s) 791, 1413, 2542
Vha464I CTTAAG 2 cut(s) 284, 3068
VneI GTGCAC 2 cut(s) 271, 1144
VpaK11BI GGWCC 3 cut(s) 661, 713, 2889
VspI ATTAAT 2 cut(s) 947, 1019
XagI CCTNNNNNAGG 2 cut(s) 732, 2532
XapI RAATTY 7 cut(s) 279, 962, 997, 1347, 1792, 1873, 1879
XmiI GTMKAC 2 cut(s) 3198, 3244
XmnI GAANNNNTTC 1 cut(s) 1562
XspI CTAG 5 cut(s) 683, 773, 977, 1002, 1830
ZrmI AGTACT 1 cut(s) 1891
Zsp2I ATGCAT 2 cut(s) 1632, 3057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.