Rmu_sc0001277.1_g000003

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001277.1
OG0021343
Physical Location & Seq
Reverse (-)
12440 .. 16668
4229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001277.1_g000003.1.cds

Sequence Viewer

Length: 1155 bp
atgactgtgaaaatcgaattggggttttcagtaatccccaagaaatcgatttggggttttcggtttctgggagatttaggggtttctgtgaaatcgaattggggttttcgatctctgggagatttgggtattactgtgaaaatcaaattgggcttttcagttaatccccaagaaatcgaattgaggttttcggtctcgtcggggagatttggggattctgcaatggaattagggttcggtgtctgggagattggggatttctgggaaatcgaattggggagaatcggcctccgatgtttgggcgttggggccttccgacgccgacttgcagggcttgatgcatcgggtcggatccaacctagtcaagcaaggctaaagcatgattggttgcaagagctggggttgtccagttatggtgggttgtttcagccatggcagaggcaattcctctgtactccgagcaaggcatcacagggcgatgtggcaggtgcacatgcggaggtttgcgtgcaagctgcagtgatacaagcggcgacgacggtattggttgtggccggagtcgttgaaggagagggggagcccatgtatggtgtgcccaaatcaggtgggaagcccaactccaagtacaggttgcctagatcttttatgtggcccaattcaatttgcatgctttgggtgtcgaaagaagagttgggggttggctatggtggggtgaaaagaacattttggaggcccaaatcgatgggcatgctgaagtccgatgtgctttgggtgtccacaacaattaagaatgttttctggacttgggcttcttggaccaaagtgttgagctatacctctaacgtcatggagatggggagagactactctggctggtttgtcaagtacaatgttgatcttgatcccttatgcaccacttccccgcagatcctacgtgctatttttgttcttctcactcttgctccggaggaaaatgaagaagatgaggaaagttcatctctgttgctgcatactcctggtaaagtcatctcttataatctgaggaataaaaccttcaaatctattgacttaactcccagggccggtgttgatgattctttttgccgaattgatttgcagaattatcgatatgttgagagtttgggtgttgtgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

384

Amino Acids

42.75

Weight (kDa)

8.78

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021343)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0174961
rosa_multiflora Rmu_sc0001277.1_g000003 Rmu_sc0009766.1_g000004
rosa_samantha Rh2CG635800 Rh2DG686300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1035
AarI CACCTGC 1 cut(s) 476
Acc36I ACCTGC 1 cut(s) 476
AccIII TCCGGA 1 cut(s) 964
AciI CCGC 3 cut(s) 497, 530, 923
AclWI GGATC 4 cut(s) 346, 359, 896, 922
AcoI YGGCCR 1 cut(s) 552
AcuI CTGAAG 1 cut(s) 773
AcyI GRCGYC 1 cut(s) 319
AfaI GTAC 3 cut(s) 454, 626, 887
AfiI CCNNNNNNNGG 5 cut(s) 298, 587, 602, 627, 1082
AgsI TTSAA 3 cut(s) 566, 660, 1057
AjnI CCWGG 2 cut(s) 1015, 1076
AjuI GAANNNNNNNTTGG 5 cut(s) 34, 131, 163, 674, 706
AloI GAACNNNNNNTCC 2 cut(s) 218, 250
AluBI AGCT 3 cut(s) 397, 515, 831
AluI AGCT 3 cut(s) 397, 515, 831
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 2 cut(s) 199, 855
Alw44I GTGCAC 1 cut(s) 489
AlwI GGATC 4 cut(s) 346, 359, 896, 922
Aor13HI TCCGGA 1 cut(s) 964
AoxI GGCC 6 cut(s) 286, 309, 552, 650, 731, 1080
ApaLI GTGCAC 1 cut(s) 489
ApeKI GCWGC 2 cut(s) 515, 1006
Asp700I GAANNNNTTC 1 cut(s) 794
AspS9I GGNCC 5 cut(s) 309, 651, 732, 816, 1080
AsuHPI GGTGA 1 cut(s) 724
AvaII GGWCC 1 cut(s) 816
BaeGI GKGCMC 2 cut(s) 493, 597
BamHI GGATCC 1 cut(s) 351
BanII GRGCYC 1 cut(s) 582
Bbv12I GWGCWC 1 cut(s) 493
BbvI GCAGC 2 cut(s) 502, 993
BccI CCATC 2 cut(s) 736, 847
BcgI CGANNNNNNTGC 4 cut(s) 730, 764, 1106, 1140
BciT130I CCWGG 2 cut(s) 1017, 1078
BcoDI GTCTC 2 cut(s) 199, 855
BfaI CTAG 2 cut(s) 360, 636
BfmI CTRYAG 1 cut(s) 516
BfuAI ACCTGC 1 cut(s) 476
BglII AGATCT 1 cut(s) 638
BisI GCNGC 3 cut(s) 516, 531, 1007
BlsI GCNGC 3 cut(s) 517, 532, 1008
Bme1390I CCNGG 2 cut(s) 1017, 1078
Bme18I GGWCC 1 cut(s) 816
BmgT120I GGNCC 5 cut(s) 309, 651, 732, 816, 1080
BmiI GGNNCC 3 cut(s) 310, 353, 579
BmrFI CCNGG 2 cut(s) 1017, 1078
BmsI GCATC 3 cut(s) 328, 350, 476
Bsa29I ATCGAT 3 cut(s) 47, 740, 1126
BsaAI YACGTR 1 cut(s) 935
BsaBI GATNNNNATC 1 cut(s) 900
BsaHI GRCGYC 1 cut(s) 319
BsaI GGTCTC 1 cut(s) 199
BsaJI CCNNGG 3 cut(s) 431, 1076, 1077
BsaWI WCCGGW 1 cut(s) 964
BsaXI ACNNNNNCTCC 4 cut(s) 569, 599, 946, 976
Bsc4I CCNNNNNNNGG 5 cut(s) 298, 587, 602, 627, 1082
Bse118I RCCGGY 1 cut(s) 1082
Bse1I ACTGG 1 cut(s) 408
Bse3DI GCAATG 1 cut(s) 228
Bse8I GATNNNNATC 1 cut(s) 900
BseAI TCCGGA 1 cut(s) 964
BseBI CCWGG 2 cut(s) 1017, 1078
BseCI ATCGAT 3 cut(s) 47, 740, 1126
BseDI CCNNGG 3 cut(s) 431, 1076, 1077
BseJI GATNNNNATC 1 cut(s) 900
BseLI CCNNNNNNNGG 5 cut(s) 298, 587, 602, 627, 1082
BseMI GCAATG 1 cut(s) 228
BseMII CTCAG 1 cut(s) 1031
BseNI ACTGG 1 cut(s) 408
BseSI GKGCMC 2 cut(s) 493, 597
BseXI GCAGC 2 cut(s) 502, 993
BseYI CCCAGC 1 cut(s) 397
BshFI GGCC 6 cut(s) 288, 311, 554, 652, 733, 1082
BshVI ATCGAT 3 cut(s) 47, 740, 1126
BsiHKAI GWGCWC 1 cut(s) 493
BsiSI CCGG 3 cut(s) 555, 965, 1083
BslI CCNNNNNNNGG 5 cut(s) 298, 587, 602, 627, 1082
BsmAI GTCTC 2 cut(s) 199, 855
BsnI GGCC 6 cut(s) 288, 311, 554, 652, 733, 1082
Bso31I GGTCTC 1 cut(s) 199
Bsp1286I GDGCHC 3 cut(s) 493, 582, 597
Bsp13I TCCGGA 1 cut(s) 964
Bsp143I GATC 6 cut(s) 110, 351, 638, 895, 901, 927
Bsp19I CCATGG 1 cut(s) 431
BspACI CCGC 3 cut(s) 497, 530, 923
BspANI GGCC 6 cut(s) 288, 311, 554, 652, 733, 1082
BspCNI CTCAG 1 cut(s) 1032
BspDI ATCGAT 3 cut(s) 47, 740, 1126
BspEI TCCGGA 1 cut(s) 964
BspLI GGNNCC 3 cut(s) 310, 353, 579
BspMAI CTGCAG 1 cut(s) 520
BspMI ACCTGC 1 cut(s) 476
BspPI GGATC 4 cut(s) 346, 359, 896, 922
BspTNI GGTCTC 1 cut(s) 199
BsrDI GCAATG 1 cut(s) 228
BsrFI RCCGGY 1 cut(s) 1082
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 1 cut(s) 1082
BssECI CCNNGG 3 cut(s) 431, 1076, 1077
BssMI GATC 6 cut(s) 110, 351, 638, 895, 901, 927
BssNI GRCGYC 1 cut(s) 319
BssT1I CCWWGG 1 cut(s) 431
Bst2UI CCWGG 2 cut(s) 1017, 1078
Bst4CI ACNGT 3 cut(s) 7, 136, 541
Bst6I CTCTTC 1 cut(s) 681
BstACI GRCGYC 1 cut(s) 319
BstBAI YACGTR 1 cut(s) 935
BstC8I GCNNGC 4 cut(s) 509, 513, 668, 749
BstDEI CTNAG 1 cut(s) 1040
BstDSI CCRYGG 1 cut(s) 431
BstKTI GATC 6 cut(s) 113, 354, 641, 898, 904, 930
BstMAI GTCTC 2 cut(s) 199, 855
BstMBI GATC 6 cut(s) 110, 351, 638, 895, 901, 927
BstNI CCWGG 2 cut(s) 1017, 1078
BstNSI RCATGY 3 cut(s) 497, 670, 751
BstSCI CCNGG 2 cut(s) 1015, 1076
BstSFI CTRYAG 1 cut(s) 516
BstSLI GKGCMC 2 cut(s) 493, 597
BstV1I GCAGC 2 cut(s) 502, 993
BstX2I RGATCY 3 cut(s) 351, 638, 927
BstXI CCANNNNNNTGG 1 cut(s) 742
BstYI RGATCY 3 cut(s) 351, 638, 927
Bsu15I ATCGAT 3 cut(s) 47, 740, 1126
BsuRI GGCC 6 cut(s) 288, 311, 554, 652, 733, 1082
BsuTUI ATCGAT 3 cut(s) 47, 740, 1126
BtgI CCRYGG 1 cut(s) 431
BtgZI GCGATG 1 cut(s) 492
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 1 cut(s) 525
BveI ACCTGC 1 cut(s) 476
Cac8I GCNNGC 4 cut(s) 509, 513, 668, 749
Cfr10I RCCGGY 1 cut(s) 1082
Cfr13I GGNCC 5 cut(s) 309, 651, 732, 816, 1080
ClaI ATCGAT 3 cut(s) 47, 740, 1126
CseI GACGC 1 cut(s) 327
Csp6I GTAC 3 cut(s) 453, 625, 886
CspCI CAANNNNNGTGG 2 cut(s) 586, 621
CviAII CATG 7 cut(s) 380, 432, 494, 583, 667, 748, 847
CviQI GTAC 3 cut(s) 453, 625, 886
DdeI CTNAG 1 cut(s) 1040
DpnI GATC 6 cut(s) 112, 353, 640, 897, 903, 929
DpnII GATC 6 cut(s) 110, 351, 638, 895, 901, 927
EaeI YGGCCR 1 cut(s) 552
Eam1104I CTCTTC 1 cut(s) 681
EarI CTCTTC 1 cut(s) 681
Eco130I CCWWGG 1 cut(s) 431
Eco24I GRGCYC 1 cut(s) 582
Eco31I GGTCTC 1 cut(s) 199
Eco47I GGWCC 1 cut(s) 816
Eco57I CTGAAG 1 cut(s) 773
EcoO109I RGGNCCY 1 cut(s) 309
EcoRII CCWGG 2 cut(s) 1015, 1076
EcoT14I CCWWGG 1 cut(s) 431
EcoT22I ATGCAT 1 cut(s) 343
EcoT38I GRGCYC 1 cut(s) 582
ErhI CCWWGG 1 cut(s) 431
FaeI CATG 7 cut(s) 383, 435, 497, 586, 670, 751, 850
FatI CATG 7 cut(s) 379, 431, 493, 582, 666, 747, 846
FauI CCCGC 1 cut(s) 930
Fnu4HI GCNGC 3 cut(s) 516, 531, 1007
FriOI GRGCYC 1 cut(s) 582
Fsp4HI GCNGC 3 cut(s) 516, 531, 1007
FspBI CTAG 2 cut(s) 360, 636
GluI GCNGC 3 cut(s) 516, 531, 1007
GsaI CCCAGC 1 cut(s) 401
HaeIII GGCC 6 cut(s) 288, 311, 554, 652, 733, 1082
HapII CCGG 3 cut(s) 555, 965, 1083
HgaI GACGC 1 cut(s) 327
Hin1I GRCGYC 1 cut(s) 319
Hin1II CATG 7 cut(s) 383, 435, 497, 586, 670, 751, 850
HinfI GANTC 4 cut(s) 215, 282, 558, 1094
HpaII CCGG 3 cut(s) 555, 965, 1083
HphI GGTGA 1 cut(s) 724
Hpy166II GTNNAC 2 cut(s) 491, 777
Hpy188I TCNGA 6 cut(s) 293, 317, 351, 459, 760, 1041
Hpy188III TCNNGA 3 cut(s) 799, 899, 965
Hpy8I GTNNAC 2 cut(s) 491, 777
Hpy99I CGWCG 4 cut(s) 202, 321, 538, 541
HpyAV CCTTC 3 cut(s) 322, 560, 1063
HpyCH4III ACNGT 3 cut(s) 7, 136, 541
HpyCH4IV ACGT 2 cut(s) 843, 934
HpyF3I CTNAG 1 cut(s) 1040
HpySE526I ACGT 2 cut(s) 843, 934
Hsp92I GRCGYC 1 cut(s) 319
Hsp92II CATG 7 cut(s) 383, 435, 497, 586, 670, 751, 850
Kpn2I TCCGGA 1 cut(s) 964
Kzo9I GATC 6 cut(s) 110, 351, 638, 895, 901, 927
LmnI GCTCC 2 cut(s) 577, 967
Lsp1109I GCAGC 2 cut(s) 502, 993
LweI GCATC 3 cut(s) 328, 350, 476
MaeI CTAG 2 cut(s) 360, 636
MaeII ACGT 2 cut(s) 843, 934
MalI GATC 6 cut(s) 112, 353, 640, 897, 903, 929
MboI GATC 6 cut(s) 110, 351, 638, 895, 901, 927
MboII GAAGA 4 cut(s) 698, 941, 989, 992
MflI RGATCY 3 cut(s) 351, 638, 927
MhlI GDGCHC 3 cut(s) 493, 582, 597
MlyI GAGTC 1 cut(s) 567
MmeI TCCRAC 3 cut(s) 329, 340, 379
Mph1103I ATGCAT 1 cut(s) 343
MroI TCCGGA 1 cut(s) 964
MroXI GAANNNNTTC 1 cut(s) 794
MseI TTAA 3 cut(s) 162, 786, 1070
MslI CAYNNNNRTG 1 cut(s) 851
MspI CCGG 3 cut(s) 555, 965, 1083
MspR9I CCNGG 2 cut(s) 1017, 1078
MvaI CCWGG 2 cut(s) 1017, 1078
NcoI CCATGG 1 cut(s) 431
NdeII GATC 6 cut(s) 110, 351, 638, 895, 901, 927
NlaIII CATG 7 cut(s) 383, 435, 497, 586, 670, 751, 850
NlaIV GGNNCC 3 cut(s) 310, 353, 579
NsiI ATGCAT 1 cut(s) 343
NspI RCATGY 3 cut(s) 497, 670, 751
PaeI GCATGC 2 cut(s) 670, 751
PaqCI CACCTGC 1 cut(s) 476
PasI CCCWGGG 1 cut(s) 1077
PdmI GAANNNNTTC 1 cut(s) 794
PfeI GAWTC 3 cut(s) 215, 282, 1094
PkrI GCNGC 3 cut(s) 517, 532, 1008
PleI GAGTC 1 cut(s) 566
PpsI GAGTC 1 cut(s) 566
Ppu21I YACGTR 1 cut(s) 935
PsiI TTATAA 1 cut(s) 1035
Psp6I CCWGG 2 cut(s) 1015, 1076
PspFI CCCAGC 1 cut(s) 397
PspGI CCWGG 2 cut(s) 1015, 1076
PspN4I GGNNCC 3 cut(s) 310, 353, 579
PspPI GGNCC 5 cut(s) 309, 651, 732, 816, 1080
PstI CTGCAG 1 cut(s) 520
PsuI RGATCY 3 cut(s) 351, 638, 927
RsaI GTAC 3 cut(s) 454, 626, 887
RsaNI GTAC 3 cut(s) 453, 625, 886
RseI CAYNNNNRTG 1 cut(s) 851
SaqAI TTAA 3 cut(s) 162, 786, 1070
SatI GCNGC 3 cut(s) 516, 531, 1007
Sau3AI GATC 6 cut(s) 110, 351, 638, 895, 901, 927
Sau96I GGNCC 5 cut(s) 309, 651, 732, 816, 1080
SchI GAGTC 1 cut(s) 567
ScrFI CCNGG 2 cut(s) 1017, 1078
SduI GDGCHC 3 cut(s) 493, 582, 597
SfaNI GCATC 3 cut(s) 328, 350, 476
SfcI CTRYAG 1 cut(s) 516
SinI GGWCC 1 cut(s) 816
SmiMI CAYNNNNRTG 1 cut(s) 851
SphI GCATGC 2 cut(s) 670, 751
SsiI CCGC 3 cut(s) 497, 530, 923
SspMI CTAG 2 cut(s) 360, 636
StyD4I CCNGG 2 cut(s) 1015, 1076
StyI CCWWGG 1 cut(s) 431
TaaI ACNGT 3 cut(s) 7, 136, 541
TaiI ACGT 2 cut(s) 846, 937
TaqI TCGA 9 cut(s) 15, 47, 95, 109, 177, 270, 680, 740, 1126
TaqII GACCGA 1 cut(s) 181
TatI WGTACW 3 cut(s) 452, 624, 885
TauI GCSGC 1 cut(s) 533
TfiI GAWTC 3 cut(s) 215, 282, 1094
Tru1I TTAA 3 cut(s) 162, 786, 1070
Tru9I TTAA 3 cut(s) 162, 786, 1070
TscAI CASTG 1 cut(s) 525
TseI GCWGC 2 cut(s) 515, 1006
TspDTI ATGAA 2 cut(s) 984, 990
TspRI CASTG 1 cut(s) 525
VneI GTGCAC 1 cut(s) 489
VpaK11BI GGWCC 1 cut(s) 816
XceI RCATGY 3 cut(s) 497, 670, 751
XmnI GAANNNNTTC 1 cut(s) 794
XspI CTAG 2 cut(s) 360, 636
Zsp2I ATGCAT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.