Rmu_sc0001533.1_g000004

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001533.1
OG0024849
Physical Location & Seq
Reverse (-)
11164 .. 11913
750 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 750 bp
atggtgcagagattctaccccaaacgctgcagcagctgctgcttagcagaaaccatagccaacactgaagaactccttaccgaaatccttgtgcgtgtgccagctacacctctggtccgcttcaaatgtgtctccaagcactggctctctcttatcttccaccccaaattctgtcaccgccacacccttcaaaacccaaacactcccatctctgccgtcttccgtgacacatcccacccccccttttgtttcgtccttcttcctctggatgatcatcatacccgtagtagtaaccatgataaccaaaacccatctgggtcaggttcacccttcagtcctctcagtgtagttcaaagccaatacaatgatcacatcagagttatccagtcctgcaacggcctcttcttgtgccatcttttgagtgcaaaagaagtgaaccccccatattttattctcaatcccacaaccaaccagttctccacacttattcctccagctactactgttactaacgatgctggtcaacaagtagaaccctatatcatcagtattgctttggcttttgacccttccaaatcacctcattacaaggctttggctggaactctactaggtgaagcagcttcaatttcagttttcgactcgatttcggaaacttcaagcttggtaagttgctgccatctttcttgcttcgatttctgggttcgaattgcatctgtttcttttgctatacgctatacatatgactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

249

Amino Acids

27.74

Weight (kDa)

6.84

Isoelectric Point (pI)

52.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 23 - 59 1e-05 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024849)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0174511
rosa_multiflora Rmu_sc0001533.1_g000004
rosa_rugosa Rorug02G0001500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 141
AciI CCGC 2 cut(s) 118, 178
AcsI RAATTY 1 cut(s) 167
AcuI CTGAAG 2 cut(s) 87, 316
AfiI CCNNNNNNNGG 1 cut(s) 141
AgsI TTSAA 5 cut(s) 124, 191, 353, 627, 660
AluBI AGCT 5 cut(s) 36, 104, 497, 623, 663
AluI AGCT 5 cut(s) 36, 104, 497, 623, 663
Alw26I GTCTC 1 cut(s) 136
AlwNI CAGNNNCTG 2 cut(s) 36, 39
AoxI GGCC 1 cut(s) 397
ApeKI GCWGC 7 cut(s) 27, 30, 33, 36, 39, 620, 675
ApoI RAATTY 1 cut(s) 167
AspS9I GGNCC 1 cut(s) 115
AsuHPI GGTGA 4 cut(s) 167, 318, 570, 626
AsuII TTCGAA 1 cut(s) 706
AvaII GGWCC 1 cut(s) 115
BbsI GAAGAC 1 cut(s) 211
BbvI GCAGC 7 cut(s) 14, 23, 26, 42, 45, 632, 662
BccI CCATC 4 cut(s) 215, 319, 420, 687
BceAI ACGGC 2 cut(s) 200, 412
BclI TGATCA 2 cut(s) 271, 367
BcoDI GTCTC 1 cut(s) 136
BfaI CTAG 1 cut(s) 611
BfmI CTRYAG 1 cut(s) 28
BisI GCNGC 7 cut(s) 28, 31, 34, 37, 40, 621, 676
BlpI GCTNAGC 1 cut(s) 43
BlsI GCNGC 7 cut(s) 29, 32, 35, 38, 41, 622, 677
Bme18I GGWCC 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 115
BmsI GCATC 2 cut(s) 505, 722
BpiI GAAGAC 1 cut(s) 211
BpmI CTGGAG 1 cut(s) 477
Bpu1102I GCTNAGC 1 cut(s) 43
Bpu14I TTCGAA 1 cut(s) 706
BsaBI GATNNNNATC 1 cut(s) 273
BsaXI ACNNNNNCTCC 2 cut(s) 461, 491
Bsc4I CCNNNNNNNGG 1 cut(s) 141
Bse1I ACTGG 3 cut(s) 146, 385, 472
Bse8I GATNNNNATC 1 cut(s) 273
BseGI GGATG 2 cut(s) 230, 274
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 1 cut(s) 141
BseMII CTCAG 1 cut(s) 355
BseNI ACTGG 3 cut(s) 146, 385, 472
BseXI GCAGC 7 cut(s) 14, 23, 26, 42, 45, 632, 662
BsgI GTGCAG 1 cut(s) 26
BshFI GGCC 1 cut(s) 399
BslI CCNNNNNNNGG 1 cut(s) 141
BsmAI GTCTC 1 cut(s) 136
BsnI GGCC 1 cut(s) 399
Bsp119I TTCGAA 1 cut(s) 706
Bsp143I GATC 2 cut(s) 271, 367
Bsp1720I GCTNAGC 1 cut(s) 43
BspACI CCGC 2 cut(s) 118, 178
BspANI GGCC 1 cut(s) 399
BspCNI CTCAG 1 cut(s) 354
BspMAI CTGCAG 1 cut(s) 32
BspT104I TTCGAA 1 cut(s) 706
BsrI ACTGG 3 cut(s) 146, 385, 472
BssMI GATC 2 cut(s) 271, 367
Bst4CI ACNGT 1 cut(s) 505
Bst6I CTCTTC 1 cut(s) 407
BstAPI GCANNNNNTGC 2 cut(s) 36, 39
BstBI TTCGAA 1 cut(s) 706
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 2 cut(s) 43, 341
BstF5I GGATG 2 cut(s) 230, 274
BstKTI GATC 2 cut(s) 274, 370
BstMAI GTCTC 1 cut(s) 136
BstMBI GATC 2 cut(s) 271, 367
BstMWI GCNNNNNNNGC 3 cut(s) 33, 36, 39
BstSFI CTRYAG 1 cut(s) 28
BstV1I GCAGC 7 cut(s) 14, 23, 26, 42, 45, 632, 662
BstV2I GAAGAC 1 cut(s) 211
BsuRI GGCC 1 cut(s) 399
BtsCI GGATG 2 cut(s) 230, 274
BtsIMutI CAGTG 3 cut(s) 63, 139, 349
Cac8I GCNNGC 1 cut(s) 102
CaiI CAGNNNCTG 2 cut(s) 36, 39
Cfr13I GGNCC 1 cut(s) 115
CviAII CATG 1 cut(s) 296
DdeI CTNAG 2 cut(s) 43, 341
DpnI GATC 2 cut(s) 273, 369
DpnII GATC 2 cut(s) 271, 367
Eam1104I CTCTTC 1 cut(s) 407
EarI CTCTTC 1 cut(s) 407
Eco47I GGWCC 1 cut(s) 115
Eco57I CTGAAG 2 cut(s) 87, 316
FaeI CATG 1 cut(s) 299
FaiI YATR 9 cut(s) 56, 279, 297, 445, 540, 731, 738, 742, 744
FalI AAGNNNNNCTT 2 cut(s) 60, 92
FatI CATG 1 cut(s) 295
FauNDI CATATG 1 cut(s) 742
FbaI TGATCA 2 cut(s) 271, 367
Fnu4HI GCNGC 7 cut(s) 28, 31, 34, 37, 40, 621, 676
FokI GGATG 2 cut(s) 217, 281
Fsp4HI GCNGC 7 cut(s) 28, 31, 34, 37, 40, 621, 676
FspBI CTAG 1 cut(s) 611
GluI GCNGC 7 cut(s) 28, 31, 34, 37, 40, 621, 676
GsuI CTGGAG 1 cut(s) 477
HaeIII GGCC 1 cut(s) 399
Hin1II CATG 1 cut(s) 299
HincII GTYRAC 1 cut(s) 524
HindII GTYRAC 1 cut(s) 524
HindIII AAGCTT 1 cut(s) 661
HinfI GANTC 2 cut(s) 12, 641
HphI GGTGA 4 cut(s) 167, 318, 570, 626
Hpy166II GTNNAC 3 cut(s) 326, 436, 524
Hpy188I TCNGA 2 cut(s) 377, 652
Hpy188III TCNNGA 1 cut(s) 266
Hpy8I GTNNAC 3 cut(s) 326, 436, 524
HpyAV CCTTC 4 cut(s) 197, 266, 340, 579
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4V TGCA 5 cut(s) 7, 30, 393, 425, 713
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 36, 39
HpyF3I CTNAG 2 cut(s) 43, 341
Hsp92II CATG 1 cut(s) 299
Ksp22I TGATCA 2 cut(s) 271, 367
Kzo9I GATC 2 cut(s) 271, 367
Lsp1109I GCAGC 7 cut(s) 14, 23, 26, 42, 45, 632, 662
LweI GCATC 2 cut(s) 505, 722
MaeI CTAG 1 cut(s) 611
MaeIII GTNAC 4 cut(s) 173, 224, 290, 505
MalI GATC 2 cut(s) 273, 369
MboI GATC 2 cut(s) 271, 367
MboII GAAGA 5 cut(s) 80, 148, 211, 251, 394
MluCI AATT 3 cut(s) 167, 627, 708
MlyI GAGTC 1 cut(s) 635
MnlI CCTC 6 cut(s) 120, 273, 348, 410, 501, 591
MspA1I CMGCKG 1 cut(s) 36
MwoI GCNNNNNNNGC 3 cut(s) 33, 36, 39
NdeI CATATG 1 cut(s) 742
NdeII GATC 2 cut(s) 271, 367
NlaIII CATG 1 cut(s) 299
NmuCI GTSAC 2 cut(s) 173, 224
NspV TTCGAA 1 cut(s) 706
PfeI GAWTC 1 cut(s) 12
PflMI CCANNNNNTGG 1 cut(s) 141
PkrI GCNGC 7 cut(s) 29, 32, 35, 38, 41, 622, 677
PleI GAGTC 1 cut(s) 635
PpsI GAGTC 1 cut(s) 635
PspPI GGNCC 1 cut(s) 115
PstI CTGCAG 1 cut(s) 32
PstNI CAGNNNCTG 2 cut(s) 36, 39
PvuII CAGCTG 1 cut(s) 36
SatI GCNGC 7 cut(s) 28, 31, 34, 37, 40, 621, 676
Sau3AI GATC 2 cut(s) 271, 367
Sau96I GGNCC 1 cut(s) 115
SchI GAGTC 1 cut(s) 635
SetI ASST 9 cut(s) 38, 106, 112, 325, 499, 583, 616, 625, 665
SfaNI GCATC 2 cut(s) 505, 722
SfcI CTRYAG 1 cut(s) 28
SfuI TTCGAA 1 cut(s) 706
SinI GGWCC 1 cut(s) 115
Sse9I AATT 3 cut(s) 167, 627, 708
SsiI CCGC 2 cut(s) 118, 178
SspMI CTAG 1 cut(s) 611
TaaI ACNGT 1 cut(s) 505
TaqI TCGA 4 cut(s) 639, 644, 693, 706
TasI AATT 3 cut(s) 167, 627, 708
TfiI GAWTC 1 cut(s) 12
TscAI CASTG 3 cut(s) 70, 146, 349
TseFI GTSAC 2 cut(s) 173, 224
TseI GCWGC 7 cut(s) 27, 30, 33, 36, 39, 620, 675
Tsp45I GTSAC 2 cut(s) 173, 224
TspGWI ACGGA 1 cut(s) 212
TspRI CASTG 3 cut(s) 70, 146, 349
Van91I CCANNNNNTGG 1 cut(s) 141
VpaK11BI GGWCC 1 cut(s) 115
XapI RAATTY 1 cut(s) 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 311
XspI CTAG 1 cut(s) 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.