Rmu_sc0002048.1_g000002

Leucine rich repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002048.1
OG0023062
Physical Location & Seq
Forward (+)
1739 .. 4875
3137 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002048.1_g000002.1.cds

Sequence Viewer

Length: 1362 bp
atgtatgacccgtcaggtttgattttggattcgggccggatttggatccgtatttgggacccgggccgcattcaaatcctgatcttggatccgagacagctgttcatgttgatttggtatttgttctggttcttaagagttcgtctgcctaaatccgaggttctgacacccttggtcgctttcgatctcttggtgagtgaatcgcctctcctaggtggtcatatcatcggttacagttacggttactctcctatttacactgcttcagtgacatatgcagtgcagcaatgccttacggcatcaagtcacatggcattttgccacgatgatgagcgctctgccttgttgcaattcaaagctagtttcaatatagacaagtctgcttcccaagatccctatgcttatccgaaagttgcatattggggtaggctagaaggagatcctcaaaggagtaactgttgttcatgggatggtgttgaatgcaaccacgactctggccatgttattggccttgatctcgccagcagctatcttcatggttccatcaactccaatagcagtctttttcaacttgttcagttgcagaggcttgacctcctctctgacaacatcagctactctcaaataccatccaggtcactattcgagcttagaaatcttgaacatatatacctctactcaaataacttgagtgggtatgttgaattcgatgagttttccaagctcgaaaagttgagaggactttctttgtcgcataacaagttatctgtgcagatgaaaactggatttagtgctattcctccaaagcttctagtactaggattgggttcatgcaacttaacagagtttccggaatttctgaaatacactagtaaactggtggatcttgatctagctgataacattcttcagggccaaataccaaaatggatatggaattcagctcgtgaaactttgaggtttctcaacctctcccacaaccacttcactggatttgaagaaaatcaaggccgtcaatggaagtcttctgtttcaaacaatgaatacgcaggagaaattccagcaacattctgcaacacggggaccctaaatgttcttgatttgtctaacaacaacctgaatggcatgattccccaatgttttgagaatctcagcgatcttgagatattgaaactgcagaacaattcttttcatgggggtattcctcgaataggttcgaacagatgtttcgacttagaagcgattgacttgagttttaatcagctacaagggaagctcccaagatcaattgccaattgtgctcagttgatggttcttaatttgggaaataatcagatcagtgataccaatcaaataacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8

Protein Analysis

453

Amino Acids

51.17

Weight (kDa)

5.57

Isoelectric Point (pI)

40.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023062)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001226.1_g000002 Rmu_sc0002048.1_g000002 Rmu_sc0029423.1_g000001
rosa_roxburghii Rroxscaffold_7G00183090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 850
AciI CCGC 1 cut(s) 67
AclWI GGATC 7 cut(s) 40, 53, 83, 96, 386, 434, 891
AcoI YGGCCR 1 cut(s) 496
AcsI RAATTY 4 cut(s) 704, 854, 937, 1056
AcuI CTGAAG 2 cut(s) 249, 893
AfaI GTAC 1 cut(s) 816
AfeI AGCGCT 1 cut(s) 335
AfiI CCNNNNNNNGG 4 cut(s) 55, 85, 212, 1211
AflII CTTAAG 1 cut(s) 133
AhlI ACTAGT 1 cut(s) 869
AjnI CCWGG 1 cut(s) 632
Alw21I GWGCWC 1 cut(s) 1303
Alw26I GTCTC 1 cut(s) 88
AlwI GGATC 7 cut(s) 40, 53, 83, 96, 386, 434, 891
Ama87I CYCGRG 1 cut(s) 61
Aor13HI TCCGGA 1 cut(s) 850
Aor51HI AGCGCT 1 cut(s) 335
AoxI GGCC 6 cut(s) 34, 64, 496, 508, 913, 1009
ApeKI GCWGC 2 cut(s) 283, 525
ApoI RAATTY 4 cut(s) 704, 854, 937, 1056
Asp700I GAANNNNTTC 1 cut(s) 1213
AspA2I CCTAGG 1 cut(s) 211
AspLEI GCGC 1 cut(s) 336
AspS9I GGNCC 5 cut(s) 34, 58, 64, 913, 1083
AsuC2I CCSGG 2 cut(s) 62, 63
AsuHPI GGTGA 1 cut(s) 205
AsuII TTCGAA 1 cut(s) 1217
AvaI CYCGRG 1 cut(s) 61
AvaII GGWCC 2 cut(s) 58, 1083
AvrII CCTAGG 1 cut(s) 211
BalI TGGCCA 1 cut(s) 498
BamHI GGATCC 2 cut(s) 45, 88
BauI CACGAG 1 cut(s) 945
BbsI GAAGAC 1 cut(s) 1017
Bbv12I GWGCWC 1 cut(s) 1303
BbvI GCAGC 2 cut(s) 295, 537
BccI CCATC 4 cut(s) 464, 551, 637, 1303
BceAI ACGGC 2 cut(s) 312, 996
BciT130I CCWGG 1 cut(s) 634
BcnI CCSGG 2 cut(s) 62, 63
BcoDI GTCTC 1 cut(s) 88
BcuI ACTAGT 1 cut(s) 869
BfaI CTAG 7 cut(s) 212, 360, 431, 812, 818, 870, 893
BfmI CTRYAG 1 cut(s) 1175
BfoI RGCGCY 1 cut(s) 337
BfrI CTTAAG 1 cut(s) 133
BisI GCNGC 3 cut(s) 67, 284, 526
BlnI CCTAGG 1 cut(s) 211
BlsI GCNGC 3 cut(s) 68, 285, 527
BmcAI AGTACT 1 cut(s) 816
Bme1390I CCNGG 3 cut(s) 62, 63, 634
Bme18I GGWCC 2 cut(s) 58, 1083
BmeT110I CYCGRG 1 cut(s) 61
BmgT120I GGNCC 5 cut(s) 34, 58, 64, 913, 1083
BmiI GGNNCC 7 cut(s) 47, 59, 60, 90, 541, 1084, 1085
BmrFI CCNGG 3 cut(s) 62, 63, 634
BmsI GCATC 1 cut(s) 308
BpiI GAAGAC 1 cut(s) 1017
Bpu14I TTCGAA 1 cut(s) 1217
BpuEI CTTGAG 3 cut(s) 709, 1181, 1270
BpuMI CCSGG 2 cut(s) 62, 63
BsaBI GATNNNNATC 3 cut(s) 44, 888, 1347
BsaJI CCNNGG 4 cut(s) 61, 156, 171, 211
BsaWI WCCGGW 1 cut(s) 850
BsaXI ACNNNNNCTCC 2 cut(s) 442, 472
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 85, 212, 1211
Bse1I ACTGG 3 cut(s) 787, 882, 994
Bse3DI GCAATG 1 cut(s) 293
Bse8I GATNNNNATC 3 cut(s) 44, 888, 1347
BseAI TCCGGA 1 cut(s) 850
BseBI CCWGG 1 cut(s) 634
BseDI CCNNGG 4 cut(s) 61, 156, 171, 211
BseGI GGATG 2 cut(s) 475, 629
BseJI GATNNNNATC 3 cut(s) 44, 888, 1347
BseLI CCNNNNNNNGG 4 cut(s) 55, 85, 212, 1211
BseMI GCAATG 1 cut(s) 293
BseMII CTCAG 2 cut(s) 1165, 1316
BseNI ACTGG 3 cut(s) 787, 882, 994
BseRI GAGGAG 1 cut(s) 587
BseXI GCAGC 2 cut(s) 295, 537
BsgI GTGCAG 2 cut(s) 302, 791
BshFI GGCC 6 cut(s) 36, 66, 498, 510, 915, 1011
BsiHKAI GWGCWC 1 cut(s) 1303
BsiHKCI CYCGRG 1 cut(s) 61
BsiSI CCGG 3 cut(s) 37, 62, 851
BslFI GGGAC 2 cut(s) 71, 1096
BslI CCNNNNNNNGG 4 cut(s) 55, 85, 212, 1211
BsmAI GTCTC 1 cut(s) 88
BsmFI GGGAC 2 cut(s) 71, 1096
BsmI GAATGC 2 cut(s) 69, 485
BsnI GGCC 6 cut(s) 36, 66, 498, 510, 915, 1011
BsoBI CYCGRG 1 cut(s) 61
Bsp119I TTCGAA 1 cut(s) 1217
Bsp1286I GDGCHC 1 cut(s) 1303
Bsp13I TCCGGA 1 cut(s) 850
BspACI CCGC 1 cut(s) 67
BspANI GGCC 6 cut(s) 36, 66, 498, 510, 915, 1011
BspCNI CTCAG 2 cut(s) 1164, 1315
BspEI TCCGGA 1 cut(s) 850
BspLI GGNNCC 7 cut(s) 47, 59, 60, 90, 541, 1084, 1085
BspMAI CTGCAG 1 cut(s) 1179
BspPI GGATC 7 cut(s) 40, 53, 83, 96, 386, 434, 891
BspT104I TTCGAA 1 cut(s) 1217
BspTI CTTAAG 1 cut(s) 133
BsrDI GCAATG 1 cut(s) 293
BsrI ACTGG 3 cut(s) 787, 882, 994
BssECI CCNNGG 4 cut(s) 61, 156, 171, 211
BssSI CACGAG 1 cut(s) 945
BssT1I CCWWGG 2 cut(s) 171, 211
Bst2BI CACGAG 1 cut(s) 945
Bst2UI CCWGG 1 cut(s) 634
Bst4CI ACNGT 3 cut(s) 236, 242, 458
BstAFI CTTAAG 1 cut(s) 133
BstBI TTCGAA 1 cut(s) 1217
BstC8I GCNNGC 1 cut(s) 523
BstDEI CTNAG 4 cut(s) 650, 1151, 1234, 1302
BstENI CCTNNNNNAGG 2 cut(s) 210, 1209
BstF5I GGATG 2 cut(s) 475, 629
BstH2I RGCGCY 1 cut(s) 337
BstHHI GCGC 1 cut(s) 336
BstMAI GTCTC 1 cut(s) 88
BstMWI GCNNNNNNNGC 1 cut(s) 1298
BstNI CCWGG 1 cut(s) 634
BstSCI CCNGG 3 cut(s) 60, 61, 632
BstSFI CTRYAG 1 cut(s) 1175
BstV1I GCAGC 2 cut(s) 295, 537
BstV2I GAAGAC 1 cut(s) 1017
BstX2I RGATCY 5 cut(s) 45, 88, 391, 439, 883
BstXI CCANNNNNNTGG 3 cut(s) 494, 506, 989
BstYI RGATCY 5 cut(s) 45, 88, 391, 439, 883
BsuRI GGCC 6 cut(s) 36, 66, 498, 510, 915, 1011
BtsCI GGATG 2 cut(s) 475, 629
BtsI GCAGTG 2 cut(s) 258, 285
BtsIMutI CAGTG 5 cut(s) 258, 273, 285, 987, 1345
Cac8I GCNNGC 1 cut(s) 523
CfoI GCGC 1 cut(s) 336
Cfr13I GGNCC 5 cut(s) 34, 58, 64, 913, 1083
Cfr9I CCCGGG 1 cut(s) 61
Csp6I GTAC 1 cut(s) 815
CviAII CATG 9 cut(s) 106, 310, 465, 500, 536, 831, 1126, 1193, 1359
CviQI GTAC 1 cut(s) 815
DdeI CTNAG 4 cut(s) 650, 1151, 1234, 1302
EaeI YGGCCR 1 cut(s) 496
Eco130I CCWWGG 2 cut(s) 171, 211
Eco47I GGWCC 2 cut(s) 58, 1083
Eco47III AGCGCT 1 cut(s) 335
Eco57I CTGAAG 2 cut(s) 249, 893
Eco88I CYCGRG 1 cut(s) 61
EcoNI CCTNNNNNAGG 2 cut(s) 210, 1209
EcoO109I RGGNCCY 2 cut(s) 58, 1083
EcoRI GAATTC 2 cut(s) 704, 937
EcoRII CCWGG 1 cut(s) 632
EcoT14I CCWWGG 2 cut(s) 171, 211
ErhI CCWWGG 2 cut(s) 171, 211
FaeI CATG 9 cut(s) 109, 313, 468, 503, 539, 834, 1129, 1196, 1362
FaqI GGGAC 2 cut(s) 71, 1096
FatI CATG 9 cut(s) 105, 309, 464, 499, 535, 830, 1125, 1192, 1358
FauNDI CATATG 1 cut(s) 274
Fnu4HI GCNGC 3 cut(s) 67, 284, 526
FokI GGATG 2 cut(s) 482, 616
Fsp4HI GCNGC 3 cut(s) 67, 284, 526
FspBI CTAG 7 cut(s) 212, 360, 431, 812, 818, 870, 893
GlaI GCGC 1 cut(s) 335
GluI GCNGC 3 cut(s) 67, 284, 526
HaeII RGCGCY 1 cut(s) 337
HaeIII GGCC 6 cut(s) 36, 66, 498, 510, 915, 1011
HapII CCGG 3 cut(s) 37, 62, 851
HhaI GCGC 1 cut(s) 336
Hin1II CATG 9 cut(s) 109, 313, 468, 503, 539, 834, 1129, 1196, 1362
Hin6I GCGC 1 cut(s) 334
HinP1I GCGC 1 cut(s) 334
HindIII AAGCTT 1 cut(s) 806
HinfI GANTC 5 cut(s) 29, 200, 491, 1129, 1147
HpaII CCGG 3 cut(s) 37, 62, 851
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 1 cut(s) 875
Hpy188I TCNGA 7 cut(s) 93, 157, 165, 408, 604, 861, 1335
Hpy188III TCNNGA 7 cut(s) 79, 659, 851, 887, 947, 1097, 1160
Hpy8I GTNNAC 1 cut(s) 875
HpyAV CCTTC 1 cut(s) 428
HpyCH4III ACNGT 3 cut(s) 236, 242, 458
HpyF10VI GCNNNNNNNGC 1 cut(s) 1298
HpyF3I CTNAG 4 cut(s) 650, 1151, 1234, 1302
Hsp92II CATG 9 cut(s) 109, 313, 468, 503, 539, 834, 1129, 1196, 1362
HspAI GCGC 1 cut(s) 334
KflI GGGWCCC 2 cut(s) 58, 1083
Kpn2I TCCGGA 1 cut(s) 850
LmnI GCTCC 1 cut(s) 1281
Lsp1109I GCAGC 2 cut(s) 295, 537
LweI GCATC 1 cut(s) 308
MaeI CTAG 7 cut(s) 212, 360, 431, 812, 818, 870, 893
MaeIII GTNAC 7 cut(s) 230, 236, 242, 268, 305, 452, 636
MboII GAAGA 4 cut(s) 524, 899, 1010, 1017
MfeI CAATTG 2 cut(s) 1287, 1294
MflI RGATCY 5 cut(s) 45, 88, 391, 439, 883
MhlI GDGCHC 1 cut(s) 1303
MlsI TGGCCA 1 cut(s) 498
MluCI AATT 9 cut(s) 350, 704, 854, 937, 1056, 1183, 1287, 1294, 1318
MluNI TGGCCA 1 cut(s) 498
MlyI GAGTC 1 cut(s) 485
Mox20I TGGCCA 1 cut(s) 498
MroI TCCGGA 1 cut(s) 850
MroXI GAANNNNTTC 1 cut(s) 1213
MscI TGGCCA 1 cut(s) 498
MseI TTAA 4 cut(s) 134, 839, 1257, 1317
MslI CAYNNNNRTG 1 cut(s) 327
Msp20I TGGCCA 1 cut(s) 498
MspA1I CMGCKG 1 cut(s) 100
MspCI CTTAAG 1 cut(s) 133
MspI CCGG 3 cut(s) 37, 62, 851
MspR9I CCNGG 3 cut(s) 62, 63, 634
MunI CAATTG 2 cut(s) 1287, 1294
Mva1269I GAATGC 2 cut(s) 69, 485
MvaI CCWGG 1 cut(s) 634
MwoI GCNNNNNNNGC 1 cut(s) 1298
NciI CCSGG 2 cut(s) 62, 63
NdeI CATATG 1 cut(s) 274
NlaIII CATG 9 cut(s) 109, 313, 468, 503, 539, 834, 1129, 1196, 1362
NlaIV GGNNCC 7 cut(s) 47, 59, 60, 90, 541, 1084, 1085
NmuCI GTSAC 3 cut(s) 268, 305, 636
NspV TTCGAA 1 cut(s) 1217
PctI GAATGC 2 cut(s) 69, 485
PdmI GAANNNNTTC 1 cut(s) 1213
PfeI GAWTC 4 cut(s) 29, 200, 1129, 1147
PkrI GCNGC 3 cut(s) 68, 285, 527
PleI GAGTC 1 cut(s) 485
PpsI GAGTC 1 cut(s) 485
PpuMI RGGWCCY 2 cut(s) 58, 1083
Psp5II RGGWCCY 2 cut(s) 58, 1083
Psp6I CCWGG 1 cut(s) 632
PspGI CCWGG 1 cut(s) 632
PspN4I GGNNCC 7 cut(s) 47, 59, 60, 90, 541, 1084, 1085
PspPI GGNCC 5 cut(s) 34, 58, 64, 913, 1083
PspPPI RGGWCCY 2 cut(s) 58, 1083
PsrI GAACNNNNNNTAC 2 cut(s) 445, 477
PstI CTGCAG 1 cut(s) 1179
PsuI RGATCY 5 cut(s) 45, 88, 391, 439, 883
PvuII CAGCTG 1 cut(s) 100
RsaI GTAC 1 cut(s) 816
RsaNI GTAC 1 cut(s) 815
RseI CAYNNNNRTG 1 cut(s) 327
SaqAI TTAA 4 cut(s) 134, 839, 1257, 1317
SatI GCNGC 3 cut(s) 67, 284, 526
Sau96I GGNCC 5 cut(s) 34, 58, 64, 913, 1083
ScaI AGTACT 1 cut(s) 816
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 3 cut(s) 62, 63, 634
SduI GDGCHC 1 cut(s) 1303
SfaNI GCATC 1 cut(s) 308
SfcI CTRYAG 1 cut(s) 1175
SfuI TTCGAA 1 cut(s) 1217
SinI GGWCC 2 cut(s) 58, 1083
SmaI CCCGGG 1 cut(s) 63
SmiMI CAYNNNNRTG 1 cut(s) 327
SmlI CTYRAG 4 cut(s) 133, 688, 1160, 1249
SmoI CTYRAG 4 cut(s) 133, 688, 1160, 1249
SpeI ACTAGT 1 cut(s) 869
Sse9I AATT 9 cut(s) 350, 704, 854, 937, 1056, 1183, 1287, 1294, 1318
SsiI CCGC 1 cut(s) 67
SspMI CTAG 7 cut(s) 212, 360, 431, 812, 818, 870, 893
StyD4I CCNGG 3 cut(s) 60, 61, 632
StyI CCWWGG 2 cut(s) 171, 211
TaaI ACNGT 3 cut(s) 236, 242, 458
TaqI TCGA 7 cut(s) 183, 645, 708, 726, 1207, 1217, 1230
TasI AATT 9 cut(s) 350, 704, 854, 937, 1056, 1183, 1287, 1294, 1318
TatI WGTACW 1 cut(s) 814
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 4 cut(s) 29, 200, 1129, 1147
Tru1I TTAA 4 cut(s) 134, 839, 1257, 1317
Tru9I TTAA 4 cut(s) 134, 839, 1257, 1317
TscAI CASTG 5 cut(s) 265, 273, 285, 994, 1345
TseFI GTSAC 3 cut(s) 268, 305, 636
TseI GCWGC 2 cut(s) 283, 525
Tsp45I GTSAC 3 cut(s) 268, 305, 636
TspDTI ATGAA 7 cut(s) 94, 453, 524, 791, 819, 1056, 1181
TspGWI ACGGA 1 cut(s) 38
TspMI CCCGGG 1 cut(s) 61
TspRI CASTG 5 cut(s) 265, 273, 285, 994, 1345
Vha464I CTTAAG 1 cut(s) 133
VpaK11BI GGWCC 2 cut(s) 58, 1083
XagI CCTNNNNNAGG 2 cut(s) 210, 1209
XapI RAATTY 4 cut(s) 704, 854, 937, 1056
XcmI CCANNNNNNNNNTGG 1 cut(s) 930
XmaI CCCGGG 1 cut(s) 61
XmaJI CCTAGG 1 cut(s) 211
XmnI GAANNNNTTC 1 cut(s) 1213
XspI CTAG 7 cut(s) 212, 360, 431, 812, 818, 870, 893
ZrmI AGTACT 1 cut(s) 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.