Rmu_sc0002157.1_g000029

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002157.1
OG0021517
Physical Location & Seq
Reverse (-)
122107 .. 123428
1322 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1134 bp
atgggattattaccactccttttaggtgtgttactactactcctagagacttcaggaaagctgccccaatcactgcctcattgccaggaccaatgtggtaacgtgtccataccatatccctttggtataaccaagggatgttattggtccccagatttcctgattcattgtattattagtggtactggatccccagtaccgtatttttggggccgtcaaatcactaatatagctcctgagacaagtgagttacacgtcttggagggtgtaacttcgacgtgccataatgagactcagatagtcattgatgagaagaccgagctacagttgcctctgcatttctcgatatctcaggcaaagaatagcctcttcggcgtcgggtgtggtaccacagcaacttttagggaggaaaatgatagctacataagacatggctcatgggaaatcgagtatactagtttttgcacttctagatgtgatgacagtctagatgacttccagctacggaggggcactatgtgttctggatttggttgttgtcgcgtatccatttcgaaggcagtgcagaacttgaccatcgtggtgagaaatagtcagaacgagaccgagtggtactacaaaagatacccatgcagttatgcattcgttgcagaaaatgaccacttcttattctctcccactacgagcttagacgatcttagcagtagaacccatcttcctatggtccttaattgggatatcggtggtggtacatgtagcacgaggagtccagcatgcagcttgtttagcaagtgcatagacactagtcgacgttcaggtgcttatatttgccagtgtttgtcgggttacagaggaaacccatacctcccagatgggtgtcatgacattgacgagtgcaagcgaaacccttgcacaataggaacttgcaccaatacaccaggtagttacttctgctctgtaagcgaacagaggcgatggattattattgtttcaggtgtgattgcaattgctctactcattttcgtcatcctttggttgagaggatttagatgttgtttgagatcgtctgaagaacaaggtaagttttattttggcaatgttacttctttttgttggtcttacctaaatgaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

377

Amino Acids

42.19

Weight (kDa)

5.87

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 28 - 85 8.4e-08 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021517)

Species Orthologous Gene IDs
rosa_laevigata RLG00000010190
rosa_multiflora Rmu_sc0002157.1_g000029 Rmu_sc0007400.1_g000004 Rmu_sc0038730.1_g000001
rosa_samantha Rh4BG004900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 386
AccB1I GGYRCC 1 cut(s) 386
AccI GTMKAC 2 cut(s) 452, 808
AccII CGCG 1 cut(s) 543
AclWI GGATC 2 cut(s) 183, 196
AcuI CTGAAG 2 cut(s) 36, 1089
AcyI GRCGYC 1 cut(s) 375
AdeI CACNNNGTG 1 cut(s) 519
AfaI GTAC 5 cut(s) 184, 198, 388, 614, 751
AfiI CCNNNNNNNGG 1 cut(s) 733
AflIII ACRYGT 3 cut(s) 102, 253, 752
AhlI ACTAGT 2 cut(s) 455, 803
AjiI CACGTC 2 cut(s) 256, 279
AjnI CCWGG 2 cut(s) 84, 937
AloI GAACNNNNNNTCC 2 cut(s) 700, 732
AluBI AGCT 7 cut(s) 61, 233, 322, 420, 502, 687, 780
AluI AGCT 7 cut(s) 61, 233, 322, 420, 502, 687, 780
Alw26I GTCTC 4 cut(s) 41, 233, 284, 596
AlwI GGATC 2 cut(s) 183, 196
AoxI GGCC 1 cut(s) 211
ApeKI GCWGC 2 cut(s) 61, 777
Asp718I GGTACC 1 cut(s) 386
AspS9I GGNCC 4 cut(s) 88, 147, 211, 724
AsuHPI GGTGA 1 cut(s) 595
AsuII TTCGAA 1 cut(s) 554
AvaII GGWCC 3 cut(s) 88, 147, 724
BaeGI GKGCMC 1 cut(s) 515
BamHI GGATCC 1 cut(s) 188
BanI GGYRCC 1 cut(s) 386
BauI CACGAG 1 cut(s) 760
BbsI GAAGAC 1 cut(s) 320
BbvI GCAGC 2 cut(s) 48, 789
BccI CCATC 4 cut(s) 584, 720, 866, 969
BceAI ACGGC 1 cut(s) 198
BcgI CGANNNNNNTGC 4 cut(s) 544, 578, 891, 925
BciT130I CCWGG 2 cut(s) 86, 939
BciVI GTATCC 1 cut(s) 556
BcoDI GTCTC 4 cut(s) 41, 233, 284, 596
BcuI ACTAGT 2 cut(s) 455, 803
BfaI CTAG 5 cut(s) 44, 456, 471, 488, 804
BfmI CTRYAG 1 cut(s) 323
BfuI GTATCC 1 cut(s) 556
BglI GCCNNNNNGGC 1 cut(s) 372
BisI GCNGC 2 cut(s) 62, 778
BlsI GCNGC 2 cut(s) 63, 779
Bme1390I CCNGG 2 cut(s) 86, 939
Bme18I GGWCC 3 cut(s) 88, 147, 724
BmgBI CACGTC 2 cut(s) 256, 279
BmgT120I GGNCC 4 cut(s) 88, 147, 211, 724
BmiI GGNNCC 4 cut(s) 149, 190, 212, 388
BmrFI CCNGG 2 cut(s) 86, 939
BmrI ACTGGG 1 cut(s) 188
BmuI ACTGGG 1 cut(s) 188
BoxI GACNNNNGTC 1 cut(s) 804
BpiI GAAGAC 1 cut(s) 320
Bpu14I TTCGAA 1 cut(s) 554
BsaHI GRCGYC 1 cut(s) 375
BsaI GGTCTC 1 cut(s) 596
BsaJI CCNNGG 1 cut(s) 132
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bsc4I CCNNNNNNNGG 1 cut(s) 733
Bse1I ACTGG 3 cut(s) 190, 194, 832
Bse3DI GCAATG 2 cut(s) 79, 1102
BseBI CCWGG 2 cut(s) 86, 939
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 2 cut(s) 143, 1026
BseLI CCNNNNNNNGG 1 cut(s) 733
BseMI GCAATG 2 cut(s) 79, 1102
BseMII CTCAG 3 cut(s) 228, 308, 365
BseNI ACTGG 3 cut(s) 190, 194, 832
BseRI GAGGAG 1 cut(s) 778
BseSI GKGCMC 1 cut(s) 515
BseXI GCAGC 2 cut(s) 48, 789
BsgI GTGCAG 1 cut(s) 584
Bsh1236I CGCG 1 cut(s) 543
BshFI GGCC 1 cut(s) 213
BshNI GGYRCC 1 cut(s) 386
BslFI GGGAC 1 cut(s) 133
BslI CCNNNNNNNGG 1 cut(s) 733
BsmAI GTCTC 4 cut(s) 41, 233, 284, 596
BsmFI GGGAC 1 cut(s) 133
BsmI GAATGC 1 cut(s) 641
BsnI GGCC 1 cut(s) 213
Bso31I GGTCTC 1 cut(s) 596
Bsp119I TTCGAA 1 cut(s) 554
Bsp1286I GDGCHC 1 cut(s) 515
Bsp143I GATC 3 cut(s) 188, 694, 1061
BspANI GGCC 1 cut(s) 213
BspCNI CTCAG 3 cut(s) 229, 307, 364
BspFNI CGCG 1 cut(s) 543
BspHI TCATGA 1 cut(s) 880
BspLI GGNNCC 4 cut(s) 149, 190, 212, 388
BspPI GGATC 2 cut(s) 183, 196
BspT104I TTCGAA 1 cut(s) 554
BspT107I GGYRCC 1 cut(s) 386
BspTNI GGTCTC 1 cut(s) 596
BsrDI GCAATG 2 cut(s) 79, 1102
BsrI ACTGG 3 cut(s) 190, 194, 832
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 3 cut(s) 188, 694, 1061
BssNAI GTATAC 1 cut(s) 453
BssNI GRCGYC 1 cut(s) 375
BssSI CACGAG 1 cut(s) 760
BssT1I CCWWGG 1 cut(s) 132
Bst1107I GTATAC 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 760
Bst2UI CCWGG 2 cut(s) 86, 939
Bst4CI ACNGT 3 cut(s) 201, 327, 485
Bst6I CTCTTC 1 cut(s) 374
BstACI GRCGYC 1 cut(s) 375
BstAPI GCANNNNNTGC 1 cut(s) 647
BstBI TTCGAA 1 cut(s) 554
BstC8I GCNNGC 2 cut(s) 775, 899
BstDEI CTNAG 5 cut(s) 237, 294, 351, 688, 698
BstF5I GGATG 2 cut(s) 143, 1026
BstFNI CGCG 1 cut(s) 543
BstKTI GATC 3 cut(s) 191, 697, 1064
BstMAI GTCTC 4 cut(s) 41, 233, 284, 596
BstMBI GATC 3 cut(s) 188, 694, 1061
BstMWI GCNNNNNNNGC 5 cut(s) 328, 372, 647, 786, 960
BstNI CCWGG 2 cut(s) 86, 939
BstNSI RCATGY 2 cut(s) 756, 777
BstPAI GACNNNNGTC 1 cut(s) 804
BstSCI CCNGG 2 cut(s) 84, 937
BstSFI CTRYAG 1 cut(s) 323
BstSLI GKGCMC 1 cut(s) 515
BstUI CGCG 1 cut(s) 543
BstV1I GCAGC 2 cut(s) 48, 789
BstV2I GAAGAC 1 cut(s) 320
BstX2I RGATCY 1 cut(s) 188
BstYI RGATCY 1 cut(s) 188
BstZ17I GTATAC 1 cut(s) 453
BsuI GTATCC 1 cut(s) 556
BsuRI GGCC 1 cut(s) 213
BtgZI GCGATG 1 cut(s) 988
BtrI CACGTC 2 cut(s) 256, 279
BtsCI GGATG 2 cut(s) 143, 1026
BtsI GCAGTG 2 cut(s) 71, 567
BtsIMutI CAGTG 3 cut(s) 71, 567, 839
Cac8I GCNNGC 2 cut(s) 775, 899
CciI TCATGA 1 cut(s) 880
Cfr13I GGNCC 4 cut(s) 88, 147, 211, 724
CseI GACGC 1 cut(s) 364
CsiI ACCWGGT 1 cut(s) 937
Csp6I GTAC 5 cut(s) 183, 197, 387, 613, 750
CviAII CATG 6 cut(s) 431, 438, 630, 753, 774, 881
CviQI GTAC 5 cut(s) 183, 197, 387, 613, 750
DdeI CTNAG 5 cut(s) 237, 294, 351, 688, 698
DpnI GATC 3 cut(s) 190, 696, 1063
DpnII GATC 3 cut(s) 188, 694, 1061
DraIII CACNNNGTG 1 cut(s) 519
Eam1104I CTCTTC 1 cut(s) 374
EarI CTCTTC 1 cut(s) 374
Eco130I CCWWGG 1 cut(s) 132
Eco31I GGTCTC 1 cut(s) 596
Eco32I GATATC 2 cut(s) 348, 739
Eco47I GGWCC 3 cut(s) 88, 147, 724
Eco57I CTGAAG 2 cut(s) 36, 1089
EcoRII CCWGG 2 cut(s) 84, 937
EcoRV GATATC 2 cut(s) 348, 739
EcoT14I CCWWGG 1 cut(s) 132
EcoT22I ATGCAT 1 cut(s) 643
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 6 cut(s) 434, 441, 633, 756, 777, 884
FaqI GGGAC 1 cut(s) 133
FatI CATG 6 cut(s) 430, 437, 629, 752, 773, 880
FblI GTMKAC 2 cut(s) 452, 808
Fnu4HI GCNGC 2 cut(s) 62, 778
FokI GGATG 2 cut(s) 150, 1013
Fsp4HI GCNGC 2 cut(s) 62, 778
FspBI CTAG 5 cut(s) 44, 456, 471, 488, 804
GluI GCNGC 2 cut(s) 62, 778
HaeIII GGCC 1 cut(s) 213
HgaI GACGC 1 cut(s) 364
Hin1I GRCGYC 1 cut(s) 375
Hin1II CATG 6 cut(s) 434, 441, 633, 756, 777, 884
HincII GTYRAC 1 cut(s) 809
HindII GTYRAC 1 cut(s) 809
HinfI GANTC 3 cut(s) 163, 292, 766
HphI GGTGA 1 cut(s) 595
Hpy166II GTNNAC 2 cut(s) 453, 809
Hpy188I TCNGA 3 cut(s) 297, 597, 1069
Hpy188III TCNNGA 8 cut(s) 54, 160, 236, 343, 471, 488, 525, 881
Hpy8I GTNNAC 2 cut(s) 453, 809
Hpy99I CGWCG 3 cut(s) 280, 380, 813
HpyAV CCTTC 1 cut(s) 550
HpyCH4III ACNGT 3 cut(s) 201, 327, 485
HpyCH4IV ACGT 4 cut(s) 102, 255, 278, 811
HpyF10VI GCNNNNNNNGC 5 cut(s) 328, 372, 647, 786, 960
HpyF3I CTNAG 5 cut(s) 237, 294, 351, 688, 698
HpySE526I ACGT 4 cut(s) 102, 255, 278, 811
Hsp92I GRCGYC 1 cut(s) 375
Hsp92II CATG 6 cut(s) 434, 441, 633, 756, 777, 884
KpnI GGTACC 1 cut(s) 390
Kzo9I GATC 3 cut(s) 188, 694, 1061
LmnI GCTCC 1 cut(s) 238
Lsp1109I GCAGC 2 cut(s) 48, 789
MabI ACCWGGT 1 cut(s) 937
MaeI CTAG 5 cut(s) 44, 456, 471, 488, 804
MaeII ACGT 4 cut(s) 102, 255, 278, 811
MaeIII GTNAC 7 cut(s) 30, 98, 249, 268, 845, 944, 1099
MalI GATC 3 cut(s) 190, 696, 1063
MboI GATC 3 cut(s) 188, 694, 1061
MboII GAAGA 4 cut(s) 325, 361, 707, 1082
MfeI CAATTG 1 cut(s) 1005
MflI RGATCY 1 cut(s) 188
MhlI GDGCHC 1 cut(s) 515
MluCI AATT 2 cut(s) 730, 1005
MlyI GAGTC 2 cut(s) 286, 775
Mph1103I ATGCAT 1 cut(s) 643
MseI TTAA 1 cut(s) 729
MslI CAYNNNNRTG 1 cut(s) 581
MspR9I CCNGG 2 cut(s) 86, 939
MunI CAATTG 1 cut(s) 1005
Mva1269I GAATGC 1 cut(s) 641
MvaI CCWGG 2 cut(s) 86, 939
MvnI CGCG 1 cut(s) 543
MwoI GCNNNNNNNGC 5 cut(s) 328, 372, 647, 786, 960
NdeII GATC 3 cut(s) 188, 694, 1061
NlaIII CATG 6 cut(s) 434, 441, 633, 756, 777, 884
NlaIV GGNNCC 4 cut(s) 149, 190, 212, 388
NsiI ATGCAT 1 cut(s) 643
NspI RCATGY 2 cut(s) 756, 777
NspV TTCGAA 1 cut(s) 554
PaeI GCATGC 1 cut(s) 777
PagI TCATGA 1 cut(s) 880
PciI ACATGT 1 cut(s) 752
PctI GAATGC 1 cut(s) 641
PfeI GAWTC 1 cut(s) 163
PkrI GCNGC 2 cut(s) 63, 779
PleI GAGTC 2 cut(s) 286, 774
PpsI GAGTC 2 cut(s) 286, 774
PscI ACATGT 1 cut(s) 752
PshAI GACNNNNGTC 1 cut(s) 804
Psp6I CCWGG 2 cut(s) 84, 937
PspGI CCWGG 2 cut(s) 84, 937
PspN4I GGNNCC 4 cut(s) 149, 190, 212, 388
PspPI GGNCC 4 cut(s) 88, 147, 211, 724
PsuI RGATCY 1 cut(s) 188
RsaI GTAC 5 cut(s) 184, 198, 388, 614, 751
RsaNI GTAC 5 cut(s) 183, 197, 387, 613, 750
RseI CAYNNNNRTG 1 cut(s) 581
SalI GTCGAC 1 cut(s) 807
SaqAI TTAA 1 cut(s) 729
SatI GCNGC 2 cut(s) 62, 778
Sau3AI GATC 3 cut(s) 188, 694, 1061
Sau96I GGNCC 4 cut(s) 88, 147, 211, 724
SchI GAGTC 2 cut(s) 286, 775
ScrFI CCNGG 2 cut(s) 86, 939
SduI GDGCHC 1 cut(s) 515
SexAI ACCWGGT 1 cut(s) 937
SfcI CTRYAG 1 cut(s) 323
SfuI TTCGAA 1 cut(s) 554
SinI GGWCC 3 cut(s) 88, 147, 724
SmiMI CAYNNNNRTG 1 cut(s) 581
SpeI ACTAGT 2 cut(s) 455, 803
SphI GCATGC 1 cut(s) 777
Sse9I AATT 2 cut(s) 730, 1005
SspMI CTAG 5 cut(s) 44, 456, 471, 488, 804
StyD4I CCNGG 2 cut(s) 84, 937
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 3 cut(s) 201, 327, 485
TaiI ACGT 4 cut(s) 105, 258, 281, 814
TaqI TCGA 5 cut(s) 275, 344, 447, 554, 808
TaqII GACCGA 2 cut(s) 332, 620
TasI AATT 2 cut(s) 730, 1005
TfiI GAWTC 1 cut(s) 163
Tru1I TTAA 1 cut(s) 729
Tru9I TTAA 1 cut(s) 729
TscAI CASTG 3 cut(s) 78, 567, 839
TseI GCWGC 2 cut(s) 61, 777
TspDTI ATGAA 1 cut(s) 155
TspGWI ACGGA 1 cut(s) 520
TspRI CASTG 3 cut(s) 78, 567, 839
VpaK11BI GGWCC 3 cut(s) 88, 147, 724
XbaI TCTAGA 2 cut(s) 470, 487
XceI RCATGY 2 cut(s) 756, 777
XcmI CCANNNNNNNNNTGG 1 cut(s) 92
XmiI GTMKAC 2 cut(s) 452, 808
XspI CTAG 5 cut(s) 44, 456, 471, 488, 804
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.