Rmu_sc0002310.1_g000004

Secoisolariciresinol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002310.1
OG0025531
Physical Location & Seq
Forward (+)
10429 .. 12635
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002310.1_g000004.1.cds

Sequence Viewer

Length: 570 bp
atgtcgaccacgtgtcgaacgtcgttagacgaagcgtctatccgaagtaaccgtcaaaaggcaggcacaccaatcgtcgagatagtctccgctacatacaactccactagcggaacttctccttgtttcatcttgcaagtgagaatctccgctagcggaacttccccctctacatgtcccatgcgagtccccactacgcgtaactccgttagaggaacttctccgccaaatacagtttcatgcacggatcttaaaactagggtatcaaagattgggctcgctacccaacaccctctgctcagcgcagctttccctcaacaaacaatataccgaccagacggaggtctatcttcgtcgaggagtaggggactccctagggggcctagcaggggcccacccgaaagggtagcagtgatcactggcggagccagtggcatcggattatccaccgctagactcttctccagacacggcgctaaagtagtgatcgccaacgtgcaagacaacataggccaatctgtttgtagagatttgaccattcctccaccaccttcatccattgcgacgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

189

Amino Acids

19.7

Weight (kDa)

11.01

Isoelectric Point (pI)

51.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025531)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002310.1_g000004 Rmu_sc0006888.1_g000047 Rmu_sc0012564.1_g000004

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 199
AciI CCGC 7 cut(s) 90, 111, 150, 156, 224, 423, 450
AclWI GGATC 1 cut(s) 255
AcvI CACGTG 1 cut(s) 12
AfiI CCNNNNNNNGG 3 cut(s) 58, 341, 389
AflIII ACRYGT 3 cut(s) 11, 173, 197
AhdI GACNNNNNGTC 2 cut(s) 12, 34
AjiI CACGTC 1 cut(s) 567
AjuI GAANNNNNNNTTGG 2 cut(s) 220, 252
AluBI AGCT 1 cut(s) 308
AluI AGCT 1 cut(s) 308
Alw26I GTCTC 1 cut(s) 91
AlwI GGATC 1 cut(s) 255
AoxI GGCC 3 cut(s) 380, 391, 511
ApaI GGGCCC 1 cut(s) 395
ApeKI GCWGC 1 cut(s) 305
ArsI GACNNNNNNTTYG 2 cut(s) 37, 69
AspA2I CCTAGG 1 cut(s) 374
AspLEI GCGC 2 cut(s) 305, 476
AspS9I GGNCC 3 cut(s) 380, 391, 392
AsuNHI GCTAGC 1 cut(s) 152
AvrII CCTAGG 1 cut(s) 374
BaeGI GKGCMC 1 cut(s) 395
BaeI ACNNNNGTAYC 2 cut(s) 246, 279
BanII GRGCYC 2 cut(s) 279, 395
BbrPI CACGTG 1 cut(s) 12
BbvI GCAGC 1 cut(s) 317
BceAI ACGGC 1 cut(s) 487
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BclI TGATCA 1 cut(s) 414
BcoDI GTCTC 1 cut(s) 91
BfaI CTAG 6 cut(s) 108, 153, 258, 375, 384, 453
BfoI RGCGCY 1 cut(s) 477
BisI GCNGC 1 cut(s) 306
BlnI CCTAGG 1 cut(s) 374
BlpI GCTNAGC 1 cut(s) 299
BlsI GCNGC 1 cut(s) 307
BmeRI GACNNNNNGTC 2 cut(s) 12, 34
BmgBI CACGTC 1 cut(s) 567
BmgT120I GGNCC 3 cut(s) 380, 391, 392
BmiI GGNNCC 4 cut(s) 381, 392, 393, 427
BmsI GCATC 1 cut(s) 444
BmtI GCTAGC 1 cut(s) 156
BoxI GACNNNNGTC 1 cut(s) 342
BplI GAGNNNNNCTC 2 cut(s) 71, 103
BpmI CTGGAG 1 cut(s) 448
Bpu1102I GCTNAGC 1 cut(s) 299
BsaAI YACGTR 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 374
BsaXI ACNNNNNCTCC 2 cut(s) 526, 556
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 341, 389
Bse1I ACTGG 2 cut(s) 424, 429
Bse3DI GCAATG 1 cut(s) 558
BseDI CCNNGG 1 cut(s) 374
BseGI GGATG 1 cut(s) 554
BseLI CCNNNNNNNGG 3 cut(s) 58, 341, 389
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 1 cut(s) 313
BseNI ACTGG 2 cut(s) 424, 429
BseRI GAGGAG 1 cut(s) 373
BseSI GKGCMC 1 cut(s) 395
BseXI GCAGC 1 cut(s) 317
Bsh1236I CGCG 1 cut(s) 199
BshFI GGCC 3 cut(s) 382, 393, 513
BslFI GGGAC 3 cut(s) 162, 173, 381
BslI CCNNNNNNNGG 3 cut(s) 58, 341, 389
BsmAI GTCTC 1 cut(s) 91
BsmFI GGGAC 3 cut(s) 162, 173, 381
BsnI GGCC 3 cut(s) 382, 393, 513
Bsp120I GGGCCC 1 cut(s) 391
Bsp1286I GDGCHC 2 cut(s) 279, 395
Bsp143I GATC 3 cut(s) 247, 414, 486
Bsp1720I GCTNAGC 1 cut(s) 299
BspACI CCGC 7 cut(s) 90, 111, 150, 156, 224, 423, 450
BspANI GGCC 3 cut(s) 382, 393, 513
BspCNI CTCAG 1 cut(s) 312
BspFNI CGCG 1 cut(s) 199
BspLI GGNNCC 4 cut(s) 381, 392, 393, 427
BspOI GCTAGC 1 cut(s) 156
BspPI GGATC 1 cut(s) 255
BsrDI GCAATG 1 cut(s) 558
BsrI ACTGG 2 cut(s) 424, 429
BssECI CCNNGG 1 cut(s) 374
BssMI GATC 3 cut(s) 247, 414, 486
BssT1I CCWWGG 1 cut(s) 374
Bst4CI ACNGT 2 cut(s) 53, 235
Bst6I CTCTTC 1 cut(s) 464
BstBAI YACGTR 1 cut(s) 12
BstC8I GCNNGC 3 cut(s) 64, 154, 279
BstDEI CTNAG 1 cut(s) 299
BstF5I GGATG 1 cut(s) 554
BstFNI CGCG 1 cut(s) 199
BstH2I RGCGCY 1 cut(s) 477
BstHHI GCGC 2 cut(s) 305, 476
BstKTI GATC 3 cut(s) 250, 417, 489
BstMAI GTCTC 1 cut(s) 91
BstMBI GATC 3 cut(s) 247, 414, 486
BstNSI RCATGY 1 cut(s) 177
BstPAI GACNNNNGTC 1 cut(s) 342
BstSLI GKGCMC 1 cut(s) 395
BstUI CGCG 1 cut(s) 199
BstV1I GCAGC 1 cut(s) 317
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BsuRI GGCC 3 cut(s) 382, 393, 513
BtrI CACGTC 1 cut(s) 567
BtsCI GGATG 1 cut(s) 554
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 3 cut(s) 417, 417, 436
Cac8I GCNNGC 3 cut(s) 64, 154, 279
CfoI GCGC 2 cut(s) 305, 476
Cfr13I GGNCC 3 cut(s) 380, 391, 392
CseI GACGC 1 cut(s) 24
CviAII CATG 3 cut(s) 174, 181, 240
CviJI RGCY 6 cut(s) 277, 308, 382, 393, 428, 513
CviKI_1 RGCY 6 cut(s) 277, 308, 382, 393, 428, 513
DdeI CTNAG 1 cut(s) 299
DpnI GATC 3 cut(s) 249, 416, 488
DpnII GATC 3 cut(s) 247, 414, 486
DriI GACNNNNNGTC 2 cut(s) 12, 34
Eam1104I CTCTTC 1 cut(s) 464
Eam1105I GACNNNNNGTC 2 cut(s) 12, 34
EarI CTCTTC 1 cut(s) 464
EciI GGCGGA 2 cut(s) 213, 438
Eco130I CCWWGG 1 cut(s) 374
Eco24I GRGCYC 2 cut(s) 279, 395
Eco72I CACGTG 1 cut(s) 12
EcoO109I RGGNCCY 2 cut(s) 380, 391
EcoT14I CCWWGG 1 cut(s) 374
EcoT38I GRGCYC 2 cut(s) 279, 395
ErhI CCWWGG 1 cut(s) 374
FaeI CATG 3 cut(s) 177, 184, 243
FaiI YATR 6 cut(s) 97, 175, 182, 241, 328, 509
FaqI GGGAC 3 cut(s) 162, 173, 381
FatI CATG 3 cut(s) 173, 180, 239
FbaI TGATCA 1 cut(s) 414
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 1 cut(s) 306
FokI GGATG 1 cut(s) 541
FriOI GRGCYC 2 cut(s) 279, 395
Fsp4HI GCNGC 1 cut(s) 306
FspBI CTAG 6 cut(s) 108, 153, 258, 375, 384, 453
GlaI GCGC 2 cut(s) 304, 475
GluI GCNGC 1 cut(s) 306
GsuI CTGGAG 1 cut(s) 448
HaeII RGCGCY 1 cut(s) 477
HaeIII GGCC 3 cut(s) 382, 393, 513
HgaI GACGC 1 cut(s) 24
HhaI GCGC 2 cut(s) 305, 476
Hin1II CATG 3 cut(s) 177, 184, 243
Hin6I GCGC 2 cut(s) 303, 474
HinP1I GCGC 2 cut(s) 303, 474
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 4 cut(s) 144, 186, 369, 456
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 2 cut(s) 44, 440
Hpy188III TCNNGA 2 cut(s) 79, 465
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 4 cut(s) 25, 80, 358, 568
HpyAV CCTTC 1 cut(s) 561
HpyCH4III ACNGT 2 cut(s) 53, 235
HpyCH4IV ACGT 4 cut(s) 11, 20, 495, 566
HpyCH4V TGCA 3 cut(s) 136, 243, 499
HpyF3I CTNAG 1 cut(s) 299
HpySE526I ACGT 4 cut(s) 11, 20, 495, 566
Hsp92II CATG 3 cut(s) 177, 184, 243
HspAI GCGC 2 cut(s) 303, 474
Ksp22I TGATCA 1 cut(s) 414
Kzo9I GATC 3 cut(s) 247, 414, 486
LmnI GCTCC 1 cut(s) 425
LpnPI CCDG 6 cut(s) 48, 348, 373, 405, 442, 478
Lsp1109I GCAGC 1 cut(s) 317
LweI GCATC 1 cut(s) 444
MaeI CTAG 6 cut(s) 108, 153, 258, 375, 384, 453
MaeII ACGT 4 cut(s) 11, 20, 495, 566
MaeIII GTNAC 2 cut(s) 47, 200
MalI GATC 3 cut(s) 249, 416, 488
MboI GATC 3 cut(s) 247, 414, 486
MboII GAAGA 2 cut(s) 342, 451
MflI RGATCY 1 cut(s) 247
MhlI GDGCHC 2 cut(s) 279, 395
MluI ACGCGT 1 cut(s) 197
MlyI GAGTC 3 cut(s) 195, 363, 450
MnlI CCTC 7 cut(s) 178, 206, 303, 324, 335, 351, 552
MseI TTAA 1 cut(s) 252
MvnI CGCG 1 cut(s) 199
NdeII GATC 3 cut(s) 247, 414, 486
NheI GCTAGC 1 cut(s) 152
NlaIII CATG 3 cut(s) 177, 184, 243
NlaIV GGNNCC 4 cut(s) 381, 392, 393, 427
NspI RCATGY 1 cut(s) 177
PciI ACATGT 1 cut(s) 173
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PfeI GAWTC 1 cut(s) 144
PkrI GCNGC 1 cut(s) 307
PleI GAGTC 3 cut(s) 194, 363, 450
PmaCI CACGTG 1 cut(s) 12
PmlI CACGTG 1 cut(s) 12
PpsI GAGTC 3 cut(s) 194, 363, 450
Ppu21I YACGTR 1 cut(s) 12
PscI ACATGT 1 cut(s) 173
PshAI GACNNNNGTC 1 cut(s) 342
PspCI CACGTG 1 cut(s) 12
PspN4I GGNNCC 4 cut(s) 381, 392, 393, 427
PspOMI GGGCCC 1 cut(s) 391
PspPI GGNCC 3 cut(s) 380, 391, 392
PsuI RGATCY 1 cut(s) 247
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 1 cut(s) 306
Sau3AI GATC 3 cut(s) 247, 414, 486
Sau96I GGNCC 3 cut(s) 380, 391, 392
SchI GAGTC 3 cut(s) 195, 363, 450
SduI GDGCHC 2 cut(s) 279, 395
SetI ASST 7 cut(s) 14, 23, 310, 346, 498, 553, 569
SfaNI GCATC 1 cut(s) 444
SsiI CCGC 7 cut(s) 90, 111, 150, 156, 224, 423, 450
SspMI CTAG 6 cut(s) 108, 153, 258, 375, 384, 453
StyI CCWWGG 1 cut(s) 374
TaaI ACNGT 2 cut(s) 53, 235
TaiI ACGT 4 cut(s) 14, 23, 498, 569
TaqI TCGA 4 cut(s) 5, 16, 78, 356
TfiI GAWTC 1 cut(s) 144
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 3 cut(s) 417, 424, 436
TseI GCWGC 1 cut(s) 305
TspDTI ATGAA 3 cut(s) 118, 228, 543
TspGWI ACGGA 3 cut(s) 196, 260, 354
TspRI CASTG 3 cut(s) 417, 424, 436
XceI RCATGY 1 cut(s) 177
XmaJI CCTAGG 1 cut(s) 374
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 6 cut(s) 108, 153, 258, 375, 384, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.