Rmu_sc0002784.1_g000068

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002784.1
OG0025509
Physical Location & Seq
Forward (+)
293366 .. 294990
1625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002784.1_g000068.1.cds

Sequence Viewer

Length: 660 bp
atgttgaagcggaaggcgtcacataagaagatgttctcccccatcatctccggtacgagcccctcaaggccatcaccgaccataatccagcggttgtcatcaggtctgggagcccaatctctagtccaagtcgaggagacctctctcgccaacctagattctgccgccggcgctcttcagctgttcagctctcttctgcctcctgcacttaggaatgatggagcgcttttggtcacattggatggcaagagattctcttatttcaacaactgcaggagtgatgatgatcactctttcattgatattggtgaagactggaccctctatcatcacaacaacaccaacaagaagaaggcactgatggcaatgcctgctcccaatttcaacttttgtctctccctaattccagaaagagtgtgggatgttgatttgattaaaatgaggtttgtttcttcgagtagaaacaaaatgtatgatttgattaaaataagagcctgtcatcttgcaaagaaaggctttgatgaagctatctccgagctggatactttgagtgaggagtcatacaaggacaacaccttaatcatgcaactcttgagggacaacctcatattgtggacctctgacattccggaggatggaggtaattataagttattataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

219

Amino Acids

24.62

Weight (kDa)

6.96

Isoelectric Point (pI)

58.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025509)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001353.1_g000012 Rmu_sc0002784.1_g000068
rosa_samantha Rh4CG320300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 648, 658
AccIII TCCGGA 1 cut(s) 628
AciI CCGC 3 cut(s) 10, 91, 165
AcuI CTGAAG 1 cut(s) 161
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 1 cut(s) 55
AfeI AGCGCT 1 cut(s) 225
AfiI CCNNNNNNNGG 2 cut(s) 133, 635
AgsI TTSAA 3 cut(s) 7, 265, 385
AluBI AGCT 4 cut(s) 181, 189, 527, 538
AluI AGCT 4 cut(s) 181, 189, 527, 538
Alw26I GTCTC 2 cut(s) 131, 398
Aor13HI TCCGGA 1 cut(s) 628
Aor51HI AGCGCT 1 cut(s) 225
AoxI GGCC 1 cut(s) 68
Asp700I GAANNNNTTC 1 cut(s) 32
AspLEI GCGC 2 cut(s) 173, 226
AspS9I GGNCC 2 cut(s) 318, 615
AsuHPI GGTGA 2 cut(s) 66, 320
AvaII GGWCC 2 cut(s) 318, 615
BanII GRGCYC 2 cut(s) 62, 115
BbsI GAAGAC 1 cut(s) 318
BccI CCATC 6 cut(s) 50, 79, 212, 236, 355, 629
BciVI GTATCC 1 cut(s) 535
BclI TGATCA 1 cut(s) 286
BcoDI GTCTC 2 cut(s) 131, 398
BfaI CTAG 2 cut(s) 122, 155
BfmI CTRYAG 1 cut(s) 271
BfoI RGCGCY 2 cut(s) 174, 227
BfuI GTATCC 1 cut(s) 535
BisI GCNGC 1 cut(s) 165
BlsI GCNGC 1 cut(s) 166
Bme18I GGWCC 2 cut(s) 318, 615
BmgT120I GGNCC 2 cut(s) 318, 615
BmiI GGNNCC 2 cut(s) 112, 320
BpiI GAAGAC 1 cut(s) 318
BplI GAGNNNNNCTC 2 cut(s) 125, 157
BpuEI CTTGAG 2 cut(s) 49, 613
BsaBI GATNNNNATC 1 cut(s) 285
BsaHI GRCGYC 1 cut(s) 17
BsaI GGTCTC 1 cut(s) 131
BsaWI WCCGGW 2 cut(s) 50, 628
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 635
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 1 cut(s) 320
Bse3DI GCAATG 1 cut(s) 372
Bse8I GATNNNNATC 1 cut(s) 285
BseAI TCCGGA 1 cut(s) 628
BseGI GGATG 3 cut(s) 247, 427, 640
BseJI GATNNNNATC 1 cut(s) 285
BseLI CCNNNNNNNGG 2 cut(s) 133, 635
BseMI GCAATG 1 cut(s) 372
BseNI ACTGG 1 cut(s) 320
BseRI GAGGAG 2 cut(s) 149, 569
BsgI GTGCAG 1 cut(s) 189
BshFI GGCC 1 cut(s) 70
BsiSI CCGG 3 cut(s) 51, 168, 629
BslFI GGGAC 1 cut(s) 611
BslI CCNNNNNNNGG 2 cut(s) 133, 635
BsmAI GTCTC 2 cut(s) 131, 398
BsmFI GGGAC 1 cut(s) 611
BsnI GGCC 1 cut(s) 70
Bso31I GGTCTC 1 cut(s) 131
Bsp1286I GDGCHC 2 cut(s) 62, 115
Bsp13I TCCGGA 1 cut(s) 628
Bsp143I GATC 1 cut(s) 286
BspACI CCGC 3 cut(s) 10, 91, 165
BspANI GGCC 1 cut(s) 70
BspEI TCCGGA 1 cut(s) 628
BspLI GGNNCC 2 cut(s) 112, 320
BspMAI CTGCAG 1 cut(s) 275
BspQI GCTCTTC 1 cut(s) 180
BspTNI GGTCTC 1 cut(s) 131
BsrDI GCAATG 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 1 cut(s) 320
BssAI RCCGGY 1 cut(s) 167
BssMI GATC 1 cut(s) 286
BssNI GRCGYC 1 cut(s) 17
Bst6I CTCTTC 2 cut(s) 180, 198
BstACI GRCGYC 1 cut(s) 17
BstAPI GCANNNNNTGC 1 cut(s) 371
BstC8I GCNNGC 2 cut(s) 169, 372
BstDEI CTNAG 1 cut(s) 209
BstF5I GGATG 3 cut(s) 247, 427, 640
BstH2I RGCGCY 2 cut(s) 174, 227
BstHHI GCGC 2 cut(s) 173, 226
BstKTI GATC 1 cut(s) 289
BstMAI GTCTC 2 cut(s) 131, 398
BstMBI GATC 1 cut(s) 286
BstMWI GCNNNNNNNGC 3 cut(s) 170, 362, 371
BstSFI CTRYAG 1 cut(s) 271
BstV2I GAAGAC 1 cut(s) 318
BsuI GTATCC 1 cut(s) 535
BsuRI GGCC 1 cut(s) 70
BtsCI GGATG 3 cut(s) 247, 427, 640
BtsIMutI CAGTG 1 cut(s) 356
Cac8I GCNNGC 2 cut(s) 169, 372
CfoI GCGC 2 cut(s) 173, 226
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 318, 615
CseI GACGC 1 cut(s) 6
Csp6I GTAC 1 cut(s) 54
CviAII CATG 1 cut(s) 583
CviJI RGCY 9 cut(s) 60, 70, 113, 181, 189, 494, 516, 527, 538
CviKI_1 RGCY 9 cut(s) 60, 70, 113, 181, 189, 494, 516, 527, 538
CviQI GTAC 1 cut(s) 54
DdeI CTNAG 1 cut(s) 209
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
Eam1104I CTCTTC 2 cut(s) 180, 198
EarI CTCTTC 2 cut(s) 180, 198
Eco24I GRGCYC 2 cut(s) 62, 115
Eco31I GGTCTC 1 cut(s) 131
Eco47I GGWCC 2 cut(s) 318, 615
Eco47III AGCGCT 1 cut(s) 225
Eco57I CTGAAG 1 cut(s) 161
EcoT38I GRGCYC 2 cut(s) 62, 115
FaeI CATG 1 cut(s) 586
FaiI YATR 8 cut(s) 24, 83, 474, 562, 584, 608, 648, 658
FalI AAGNNNNNCTT 2 cut(s) 500, 532
FaqI GGGAC 1 cut(s) 611
FatI CATG 1 cut(s) 582
FbaI TGATCA 1 cut(s) 286
Fnu4HI GCNGC 1 cut(s) 165
FokI GGATG 3 cut(s) 254, 434, 647
FriOI GRGCYC 2 cut(s) 62, 115
Fsp4HI GCNGC 1 cut(s) 165
FspBI CTAG 2 cut(s) 122, 155
GlaI GCGC 2 cut(s) 172, 225
GluI GCNGC 1 cut(s) 165
HaeII RGCGCY 2 cut(s) 174, 227
HaeIII GGCC 1 cut(s) 70
HapII CCGG 3 cut(s) 51, 168, 629
HgaI GACGC 1 cut(s) 6
HhaI GCGC 2 cut(s) 173, 226
Hin1I GRCGYC 1 cut(s) 17
Hin1II CATG 1 cut(s) 586
Hin6I GCGC 2 cut(s) 171, 224
HinP1I GCGC 2 cut(s) 171, 224
HinfI GANTC 3 cut(s) 158, 252, 557
HpaII CCGG 3 cut(s) 51, 168, 629
HphI GGTGA 2 cut(s) 66, 320
Hpy166II GTNNAC 1 cut(s) 615
Hpy188I TCNGA 2 cut(s) 535, 622
Hpy188III TCNNGA 3 cut(s) 407, 592, 629
Hpy8I GTNNAC 1 cut(s) 615
HpyAV CCTTC 2 cut(s) 7, 346
HpyCH4V TGCA 4 cut(s) 206, 273, 506, 586
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 362, 371
HpyF3I CTNAG 1 cut(s) 209
Hsp92I GRCGYC 1 cut(s) 17
Hsp92II CATG 1 cut(s) 586
HspAI GCGC 2 cut(s) 171, 224
Kpn2I TCCGGA 1 cut(s) 628
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Ksp22I TGATCA 1 cut(s) 286
Kzo9I GATC 1 cut(s) 286
LguI GCTCTTC 1 cut(s) 180
LmnI GCTCC 3 cut(s) 110, 221, 379
MaeI CTAG 2 cut(s) 122, 155
MaeIII GTNAC 2 cut(s) 18, 232
MalI GATC 1 cut(s) 288
MboI GATC 1 cut(s) 286
MboII GAAGA 6 cut(s) 40, 167, 185, 323, 361, 444
MhlI GDGCHC 2 cut(s) 62, 115
MluCI AATT 3 cut(s) 379, 402, 643
MlyI GAGTC 1 cut(s) 566
MreI CGCCGGCG 1 cut(s) 167
MroI TCCGGA 1 cut(s) 628
MroNI GCCGGC 1 cut(s) 167
MroXI GAANNNNTTC 1 cut(s) 32
MseI TTAA 3 cut(s) 435, 483, 578
MspA1I CMGCKG 2 cut(s) 91, 181
MspI CCGG 3 cut(s) 51, 168, 629
MwoI GCNNNNNNNGC 3 cut(s) 170, 362, 371
NaeI GCCGGC 1 cut(s) 169
NdeII GATC 1 cut(s) 286
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 1 cut(s) 586
NlaIV GGNNCC 2 cut(s) 112, 320
NmuCI GTSAC 2 cut(s) 18, 232
PciSI GCTCTTC 1 cut(s) 180
PdiI GCCGGC 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 32
PfeI GAWTC 2 cut(s) 158, 252
PkrI GCNGC 1 cut(s) 166
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
PsiI TTATAA 2 cut(s) 648, 658
PspN4I GGNNCC 2 cut(s) 112, 320
PspPI GGNCC 2 cut(s) 318, 615
PstI CTGCAG 1 cut(s) 275
PvuII CAGCTG 1 cut(s) 181
RsaI GTAC 1 cut(s) 55
RsaNI GTAC 1 cut(s) 54
SapI GCTCTTC 1 cut(s) 180
SaqAI TTAA 3 cut(s) 435, 483, 578
SatI GCNGC 1 cut(s) 165
Sau3AI GATC 1 cut(s) 286
Sau96I GGNCC 2 cut(s) 318, 615
SchI GAGTC 1 cut(s) 566
SduI GDGCHC 2 cut(s) 62, 115
SfcI CTRYAG 1 cut(s) 271
SgrAI CRCCGGYG 1 cut(s) 167
SinI GGWCC 2 cut(s) 318, 615
SmlI CTYRAG 2 cut(s) 64, 592
SmoI CTYRAG 2 cut(s) 64, 592
Sse9I AATT 3 cut(s) 379, 402, 643
SsiI CCGC 3 cut(s) 10, 91, 165
SspMI CTAG 2 cut(s) 122, 155
TaqI TCGA 2 cut(s) 132, 455
TasI AATT 3 cut(s) 379, 402, 643
TauI GCSGC 1 cut(s) 167
TfiI GAWTC 2 cut(s) 158, 252
Tru1I TTAA 3 cut(s) 435, 483, 578
Tru9I TTAA 3 cut(s) 435, 483, 578
TscAI CASTG 1 cut(s) 363
TseFI GTSAC 2 cut(s) 18, 232
Tsp45I GTSAC 2 cut(s) 18, 232
TspDTI ATGAA 2 cut(s) 286, 537
TspRI CASTG 1 cut(s) 363
VpaK11BI GGWCC 2 cut(s) 318, 615
XcmI CCANNNNNNNNNTGG 1 cut(s) 414
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 2 cut(s) 122, 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.