Rh4CG320300

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0025509
Physical Location & Seq
Reverse (-)
58238289 .. 58241172
2884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG320300.1

Sequence Viewer

Length: 582 bp
ATGAGACGATCAGTGATATTCCTTTTCCTATTTTCTCCCTTGATTGGCTGTTTCGTCAAGGCTGGTTCAGTCGAGGAGACCTCCCTCGCCAACCCAGATTCTGCCGGCGGTGCTCTTCAGCTGTTCAGCTATCTTCTGCCTCCTGCACTTAGGGATGATGGAGCGCTTTTGGTCACATTGGATGGCAAGAGCTTCTCTTATTTCAACAACTGCAGGAGTGATGATGATCACTCTTTCATTGATATTGGTGAAGACTGGACCCTCTATTGTCACAACAACACCAACAAGAAGAAGTGGGATGTTGATTTGATTAAAATGAGGTTTGTTTCTTCGAGTAGAAACAAAATGTATGATTTGATTAAAATAAGAGCCTGTCATCTTGCAAAGCAAGCCTTTGAAGAAGCTATCTCCGAGCTGGATACTTTGAGTGAGGAGTCATACAAGGACAACACCTTAATTATGCAACTCTTGAGGGACAACCTCACATTGTGGACCTCTGACATTCCCGAGGATGGAGTTGAAGAATGGCAGAAGGCAGACAACTCTGCAAAACTGGAGGTGAAGATGAAGAAATACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

193

Amino Acids

22.06

Weight (kDa)

5.02

Isoelectric Point (pI)

51.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 123 - 165 7.4e-17 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025509)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0001353.1_g000012 Rmu_sc0002784.1_g000068
rosa_samantha Rh4CG320300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 108
AcuI CTGAAG 1 cut(s) 101
AdeI CACNNNGTG 1 cut(s) 489
AfeI AGCGCT 1 cut(s) 165
AfiI CCNNNNNNNGG 2 cut(s) 44, 512
AgsI TTSAA 3 cut(s) 205, 398, 521
AluBI AGCT 5 cut(s) 121, 129, 192, 404, 415
AluI AGCT 5 cut(s) 121, 129, 192, 404, 415
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 1 cut(s) 71
AlwNI CAGNNNCTG 1 cut(s) 101
Ama87I CYCGRG 1 cut(s) 506
Aor51HI AGCGCT 1 cut(s) 165
AspLEI GCGC 1 cut(s) 166
AspS9I GGNCC 2 cut(s) 258, 492
AsuHPI GGTGA 2 cut(s) 260, 571
AvaI CYCGRG 1 cut(s) 506
AvaII GGWCC 2 cut(s) 258, 492
BbsI GAAGAC 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 115
BccI CCATC 3 cut(s) 152, 176, 506
BciVI GTATCC 1 cut(s) 412
BclI TGATCA 1 cut(s) 226
BcoDI GTCTC 1 cut(s) 71
BfmI CTRYAG 1 cut(s) 211
BfoI RGCGCY 1 cut(s) 167
BfuI GTATCC 1 cut(s) 412
Bme18I GGWCC 2 cut(s) 258, 492
BmeT110I CYCGRG 1 cut(s) 506
BmgT120I GGNCC 2 cut(s) 258, 492
BmiI GGNNCC 1 cut(s) 260
BpiI GAAGAC 1 cut(s) 258
BplI GAGNNNNNCTC 2 cut(s) 65, 97
BpmI CTGGAG 1 cut(s) 575
BpuEI CTTGAG 1 cut(s) 490
BsaBI GATNNNNATC 1 cut(s) 225
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 1 cut(s) 507
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 512
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 260, 558
Bse8I GATNNNNATC 1 cut(s) 225
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 4 cut(s) 160, 187, 304, 517
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 2 cut(s) 44, 512
BseNI ACTGG 2 cut(s) 260, 558
BseRI GAGGAG 2 cut(s) 89, 446
BsgI GTGCAG 1 cut(s) 129
BsiHKAI GWGCWC 1 cut(s) 115
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 1 cut(s) 105
BslFI GGGAC 1 cut(s) 488
BslI CCNNNNNNNGG 2 cut(s) 44, 512
BsmAI GTCTC 1 cut(s) 71
BsmFI GGGAC 1 cut(s) 488
Bso31I GGTCTC 1 cut(s) 71
BsoBI CYCGRG 1 cut(s) 506
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 2 cut(s) 8, 226
BspACI CCGC 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 260
BspMAI CTGCAG 1 cut(s) 215
BspQI GCTCTTC 1 cut(s) 120
BspTNI GGTCTC 1 cut(s) 71
BsrFI RCCGGY 1 cut(s) 104
BsrI ACTGG 2 cut(s) 260, 558
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 507
BssMI GATC 2 cut(s) 8, 226
Bst6I CTCTTC 1 cut(s) 120
BstC8I GCNNGC 2 cut(s) 106, 390
BstDEI CTNAG 1 cut(s) 149
BstF5I GGATG 4 cut(s) 160, 187, 304, 517
BstH2I RGCGCY 1 cut(s) 167
BstHHI GCGC 1 cut(s) 166
BstKTI GATC 2 cut(s) 11, 229
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 2 cut(s) 8, 226
BstMWI GCNNNNNNNGC 2 cut(s) 110, 389
BstSFI CTRYAG 1 cut(s) 211
BstV2I GAAGAC 1 cut(s) 258
BsuI GTATCC 1 cut(s) 412
BtsCI GGATG 4 cut(s) 160, 187, 304, 517
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 2 cut(s) 106, 390
CaiI CAGNNNCTG 1 cut(s) 101
CfoI GCGC 1 cut(s) 166
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 2 cut(s) 258, 492
CviJI RGCY 9 cut(s) 48, 62, 121, 129, 192, 371, 392, 404, 415
CviKI_1 RGCY 9 cut(s) 48, 62, 121, 129, 192, 371, 392, 404, 415
DdeI CTNAG 1 cut(s) 149
DpnI GATC 2 cut(s) 10, 228
DpnII GATC 2 cut(s) 8, 226
DraIII CACNNNGTG 1 cut(s) 489
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
Eco31I GGTCTC 1 cut(s) 71
Eco47I GGWCC 2 cut(s) 258, 492
Eco47III AGCGCT 1 cut(s) 165
Eco57I CTGAAG 1 cut(s) 101
Eco88I CYCGRG 1 cut(s) 506
FaiI YATR 5 cut(s) 351, 439, 461, 578, 580
FalI AAGNNNNNCTT 2 cut(s) 377, 409
FaqI GGGAC 1 cut(s) 488
FbaI TGATCA 1 cut(s) 226
FokI GGATG 4 cut(s) 167, 194, 311, 524
GlaI GCGC 1 cut(s) 165
GsuI CTGGAG 1 cut(s) 575
HaeII RGCGCY 1 cut(s) 167
HapII CCGG 1 cut(s) 105
HhaI GCGC 1 cut(s) 166
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HinfI GANTC 2 cut(s) 98, 434
HpaII CCGG 1 cut(s) 105
HphI GGTGA 2 cut(s) 260, 571
Hpy166II GTNNAC 1 cut(s) 492
Hpy188I TCNGA 2 cut(s) 412, 499
Hpy188III TCNNGA 2 cut(s) 469, 506
Hpy8I GTNNAC 1 cut(s) 492
HpyAV CCTTC 1 cut(s) 526
HpyCH4V TGCA 5 cut(s) 146, 213, 383, 463, 548
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 389
HpyF3I CTNAG 1 cut(s) 149
HspAI GCGC 1 cut(s) 164
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 2 cut(s) 8, 226
LguI GCTCTTC 1 cut(s) 120
LmnI GCTCC 1 cut(s) 161
LpnPI CCDG 9 cut(s) 48, 108, 118, 156, 199, 241, 385, 401, 539
MaeIII GTNAC 2 cut(s) 172, 269
MalI GATC 2 cut(s) 10, 228
MboI GATC 2 cut(s) 8, 226
MboII GAAGA 9 cut(s) 107, 125, 263, 301, 321, 410, 533, 574, 580
MhlI GDGCHC 1 cut(s) 115
MluCI AATT 1 cut(s) 456
MlyI GAGTC 1 cut(s) 443
MroNI GCCGGC 1 cut(s) 104
MseI TTAA 3 cut(s) 312, 360, 455
MspA1I CMGCKG 1 cut(s) 121
MspI CCGG 1 cut(s) 105
MwoI GCNNNNNNNGC 2 cut(s) 110, 389
NaeI GCCGGC 1 cut(s) 106
NdeII GATC 2 cut(s) 8, 226
NgoMIV GCCGGC 1 cut(s) 104
NlaIV GGNNCC 1 cut(s) 260
NmuCI GTSAC 2 cut(s) 172, 269
PciSI GCTCTTC 1 cut(s) 120
PdiI GCCGGC 1 cut(s) 106
PfeI GAWTC 1 cut(s) 98
PleI GAGTC 1 cut(s) 442
PpsI GAGTC 1 cut(s) 442
PspN4I GGNNCC 1 cut(s) 260
PspPI GGNCC 2 cut(s) 258, 492
PstI CTGCAG 1 cut(s) 215
PstNI CAGNNNCTG 1 cut(s) 101
PvuII CAGCTG 1 cut(s) 121
SapI GCTCTTC 1 cut(s) 120
SaqAI TTAA 3 cut(s) 312, 360, 455
Sau3AI GATC 2 cut(s) 8, 226
Sau96I GGNCC 2 cut(s) 258, 492
SchI GAGTC 1 cut(s) 443
SduI GDGCHC 1 cut(s) 115
SfcI CTRYAG 1 cut(s) 211
SinI GGWCC 2 cut(s) 258, 492
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
Sse9I AATT 1 cut(s) 456
SsiI CCGC 1 cut(s) 108
TaqI TCGA 2 cut(s) 72, 332
TasI AATT 1 cut(s) 456
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 3 cut(s) 312, 360, 455
Tru9I TTAA 3 cut(s) 312, 360, 455
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 2 cut(s) 172, 269
Tsp45I GTSAC 2 cut(s) 172, 269
TspDTI ATGAA 2 cut(s) 226, 581
TspRI CASTG 1 cut(s) 18
VpaK11BI GGWCC 2 cut(s) 258, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.