Rmu_sc0003354.1_g000022

vacuolar amino acid transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003354.1
OG0025561
Physical Location & Seq
Reverse (-)
86390 .. 87511
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 519 bp
atggaagctgaaccgctagataaatcgtcactgaccactcctcttatcgtggatgagcggcaatatgatgagccaggatgcaataccaagctagttgaagaagaactagagtccaacaattctcatcctcaaagtaaagccacaacatctactctcaaaacctgtttcaatggactcaatgctctctcaggagttgggatcctatcagttccttatgcactggcaactggagggtggtcaagcttagcacttctgtttttgttcgctgctgcagccttttactcaggcttgttaatcaagagattgcagtcctctgcatatatgctagaacttgtgttattgtgctcatcctgtgttccctaccctgtacacttctatgaaaaacaaaaaacagttctccaatgttctccttgtctgctttatattatgcaccatctgttatgcatcaatggcagtttttgggtacttaatgttcgggtcaacagttcaatcgcagataactctaaacctcccgactag

Protein Analysis

172

Amino Acids

18.86

Weight (kDa)

5.52

Isoelectric Point (pI)

50.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 48 - 102 6.8e-08 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0025561)

Species Orthologous Gene IDs
rosa_multiflora Rmu_co8269365.1_g000001 Rmu_sc0003354.1_g000022
rosa_samantha Rh2DG626200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 58
AciI CCGC 2 cut(s) 14, 58
AclWI GGATC 2 cut(s) 193, 206
AfaI GTAC 2 cut(s) 369, 465
AgsI TTSAA 3 cut(s) 98, 169, 489
AjnI CCWGG 1 cut(s) 73
AluBI AGCT 3 cut(s) 8, 91, 243
AluI AGCT 3 cut(s) 8, 91, 243
Alw21I GWGCWC 1 cut(s) 347
AlwI GGATC 2 cut(s) 193, 206
ApeKI GCWGC 3 cut(s) 266, 269, 272
BamHI GGATCC 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 347
BbvI GCAGC 3 cut(s) 253, 256, 284
BccI CCATC 1 cut(s) 441
BciT130I CCWGG 1 cut(s) 75
BfaI CTAG 5 cut(s) 17, 92, 107, 326, 517
BfmI CTRYAG 1 cut(s) 270
BisI GCNGC 4 cut(s) 59, 267, 270, 273
BlpI GCTNAGC 1 cut(s) 244
BlsI GCNGC 4 cut(s) 60, 268, 271, 274
Bme1390I CCNGG 1 cut(s) 75
BmiI GGNNCC 1 cut(s) 200
BmrFI CCNGG 1 cut(s) 75
BmsI GCATC 2 cut(s) 68, 453
BpmI CTGGAG 1 cut(s) 249
Bpu1102I GCTNAGC 1 cut(s) 244
Bse1I ACTGG 2 cut(s) 225, 232
BseBI CCWGG 1 cut(s) 75
BseGI GGATG 4 cut(s) 58, 83, 124, 347
BseMII CTCAG 2 cut(s) 201, 297
BseNI ACTGG 2 cut(s) 225, 232
BseRI GAGGAG 1 cut(s) 30
BseXI GCAGC 3 cut(s) 253, 256, 284
BsiHKAI GWGCWC 1 cut(s) 347
Bsp1286I GDGCHC 1 cut(s) 347
Bsp1407I TGTACA 1 cut(s) 367
Bsp143I GATC 1 cut(s) 198
Bsp1720I GCTNAGC 1 cut(s) 244
BspACI CCGC 2 cut(s) 14, 58
BspCNI CTCAG 2 cut(s) 200, 296
BspLI GGNNCC 1 cut(s) 200
BspMAI CTGCAG 1 cut(s) 274
BspPI GGATC 2 cut(s) 193, 206
BsrBI CCGCTC 1 cut(s) 58
BsrGI TGTACA 1 cut(s) 367
BsrI ACTGG 2 cut(s) 225, 232
BssMI GATC 1 cut(s) 198
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 2 cut(s) 394, 485
BstAUI TGTACA 1 cut(s) 367
BstDEI CTNAG 3 cut(s) 187, 244, 283
BstF5I GGATG 4 cut(s) 58, 83, 124, 347
BstKTI GATC 1 cut(s) 201
BstMBI GATC 1 cut(s) 198
BstMWI GCNNNNNNNGC 2 cut(s) 272, 450
BstNI CCWGG 1 cut(s) 75
BstSCI CCNGG 1 cut(s) 73
BstSFI CTRYAG 1 cut(s) 270
BstV1I GCAGC 3 cut(s) 253, 256, 284
BstX2I RGATCY 1 cut(s) 198
BstYI RGATCY 1 cut(s) 198
BtsCI GGATG 4 cut(s) 58, 83, 124, 347
BtsIMutI CAGTG 2 cut(s) 29, 218
Csp6I GTAC 2 cut(s) 368, 464
CviJI RGCY 7 cut(s) 8, 73, 91, 140, 243, 275, 288
CviKI_1 RGCY 7 cut(s) 8, 73, 91, 140, 243, 275, 288
CviQI GTAC 2 cut(s) 368, 464
DdeI CTNAG 3 cut(s) 187, 244, 283
DpnI GATC 1 cut(s) 200
DpnII GATC 1 cut(s) 198
EcoRII CCWGG 1 cut(s) 73
EcoT22I ATGCAT 1 cut(s) 446
FaiI YATR 9 cut(s) 66, 216, 319, 321, 323, 378, 423, 428, 442
Fnu4HI GCNGC 4 cut(s) 59, 267, 270, 273
FokI GGATG 4 cut(s) 65, 90, 111, 334
Fsp4HI GCNGC 4 cut(s) 59, 267, 270, 273
FspBI CTAG 5 cut(s) 17, 92, 107, 326, 517
GluI GCNGC 4 cut(s) 59, 267, 270, 273
GsuI CTGGAG 1 cut(s) 249
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HindIII AAGCTT 1 cut(s) 241
HinfI GANTC 2 cut(s) 110, 174
Hpy166II GTNNAC 2 cut(s) 370, 481
Hpy188III TCNNGA 3 cut(s) 189, 298, 512
Hpy8I GTNNAC 2 cut(s) 370, 481
HpyCH4III ACNGT 2 cut(s) 394, 485
HpyCH4V TGCA 7 cut(s) 81, 218, 272, 307, 317, 430, 444
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 450
HpyF3I CTNAG 3 cut(s) 187, 244, 283
Kzo9I GATC 1 cut(s) 198
LpnPI CCDG 9 cut(s) 60, 87, 174, 175, 206, 213, 270, 364, 378
Lsp1109I GCAGC 3 cut(s) 253, 256, 284
LweI GCATC 2 cut(s) 68, 453
MaeI CTAG 5 cut(s) 17, 92, 107, 326, 517
MaeIII GTNAC 1 cut(s) 27
MalI GATC 1 cut(s) 200
MbiI CCGCTC 1 cut(s) 58
MboI GATC 1 cut(s) 198
MboII GAAGA 2 cut(s) 110, 113
MflI RGATCY 1 cut(s) 198
MhlI GDGCHC 1 cut(s) 347
MluCI AATT 1 cut(s) 118
MlyI GAGTC 2 cut(s) 119, 168
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 5 cut(s) 51, 138, 224, 322, 519
Mph1103I ATGCAT 1 cut(s) 446
MseI TTAA 2 cut(s) 293, 468
MslI CAYNNNNRTG 1 cut(s) 375
MspR9I CCNGG 1 cut(s) 75
MvaI CCWGG 1 cut(s) 75
MwoI GCNNNNNNNGC 2 cut(s) 272, 450
NdeII GATC 1 cut(s) 198
NlaIV GGNNCC 1 cut(s) 200
NmuCI GTSAC 1 cut(s) 27
NsiI ATGCAT 1 cut(s) 446
PkrI GCNGC 4 cut(s) 60, 268, 271, 274
PleI GAGTC 2 cut(s) 118, 168
PpsI GAGTC 2 cut(s) 118, 168
Psp6I CCWGG 1 cut(s) 73
PspGI CCWGG 1 cut(s) 73
PspN4I GGNNCC 1 cut(s) 200
PsrI GAACNNNNNNTAC 2 cut(s) 456, 488
PstI CTGCAG 1 cut(s) 274
PsuI RGATCY 1 cut(s) 198
RsaI GTAC 2 cut(s) 369, 465
RsaNI GTAC 2 cut(s) 368, 464
RseI CAYNNNNRTG 1 cut(s) 375
SaqAI TTAA 2 cut(s) 293, 468
SatI GCNGC 4 cut(s) 59, 267, 270, 273
Sau3AI GATC 1 cut(s) 198
SchI GAGTC 2 cut(s) 119, 168
ScrFI CCNGG 1 cut(s) 75
SduI GDGCHC 1 cut(s) 347
SetI ASST 5 cut(s) 10, 93, 164, 245, 511
SfaNI GCATC 2 cut(s) 68, 453
SfcI CTRYAG 1 cut(s) 270
SmiMI CAYNNNNRTG 1 cut(s) 375
Sse9I AATT 1 cut(s) 118
SsiI CCGC 2 cut(s) 14, 58
SspMI CTAG 5 cut(s) 17, 92, 107, 326, 517
StyD4I CCNGG 1 cut(s) 73
TaaI ACNGT 2 cut(s) 394, 485
TasI AATT 1 cut(s) 118
TatI WGTACW 1 cut(s) 367
TauI GCSGC 1 cut(s) 61
Tru1I TTAA 2 cut(s) 293, 468
Tru9I TTAA 2 cut(s) 293, 468
TscAI CASTG 2 cut(s) 36, 225
TseFI GTSAC 1 cut(s) 27
TseI GCWGC 3 cut(s) 266, 269, 272
Tsp45I GTSAC 1 cut(s) 27
TspDTI ATGAA 1 cut(s) 393
TspRI CASTG 2 cut(s) 36, 225
XspI CTAG 5 cut(s) 17, 92, 107, 326, 517
Zsp2I ATGCAT 1 cut(s) 446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.