Rmu_sc0003452.1_g000011

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003452.1
OG0025562
Physical Location & Seq
Reverse (-)
64358 .. 67386
3029 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003452.1_g000011.1.cds

Sequence Viewer

Length: 510 bp
atggtcactggagcagcatatggtcaacaagggtgtctgcttagaagcctgcctgcagatgtgatgtttgacaattcaactggcttcagctttacatgtgatttaagtgataatattgcagctccatggccttcatcgtcctcaatttcatcagtgtccctctgtgggcctgagatgtcattgcccgcactaccaacatcggagacttttaaaaatcctggctgtcgtggtggagggcagcaatttcttgtaaccattatttcatttttggtcactgaagatgccgccggagactttattagagcaagttcacctgttgggtcaccagggcaggaaactattcactgccactggatctttgcttccacccgttgggtcaggtcgcagaagaatctggaaaccaacactgagggcgcggttctcatccgggtcgtgcaccaaatgaggaacgacttagagaagaaaggggcaaagtgtcgactggccagtgttcaaagatccggagtttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

169

Amino Acids

18.1

Weight (kDa)

6.81

Isoelectric Point (pI)

55.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025562)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003452.1_g000011 Rmu_sc0004066.1_g000001 Rmu_sc0041072.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 372
AccI GTMKAC 1 cut(s) 478
AccII CGCG 1 cut(s) 416
AccIII TCCGGA 1 cut(s) 500
AciI CCGC 3 cut(s) 186, 285, 416
AclWI GGATC 2 cut(s) 362, 492
AcoI YGGCCR 1 cut(s) 483
AcuI CTGAAG 2 cut(s) 70, 297
AfiI CCNNNNNNNGG 2 cut(s) 165, 372
AflIII ACRYGT 1 cut(s) 95
AgsI TTSAA 2 cut(s) 78, 494
AjnI CCWGG 2 cut(s) 217, 325
AluBI AGCT 2 cut(s) 90, 122
AluI AGCT 2 cut(s) 90, 122
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 2 cut(s) 197, 285
Alw44I GTGCAC 1 cut(s) 434
AlwI GGATC 2 cut(s) 362, 492
Aor13HI TCCGGA 1 cut(s) 500
AoxI GGCC 3 cut(s) 128, 167, 483
ApaLI GTGCAC 1 cut(s) 434
ApeKI GCWGC 3 cut(s) 14, 119, 238
Asp700I GAANNNNTTC 1 cut(s) 339
AspLEI GCGC 1 cut(s) 416
AspS9I GGNCC 1 cut(s) 167
AsuC2I CCSGG 1 cut(s) 428
AsuHPI GGTGA 2 cut(s) 303, 315
BaeGI GKGCMC 1 cut(s) 438
BalI TGGCCA 1 cut(s) 485
Bbv12I GWGCWC 1 cut(s) 438
BbvI GCAGC 3 cut(s) 26, 131, 250
BciT130I CCWGG 2 cut(s) 219, 327
BcnI CCSGG 1 cut(s) 428
BcoDI GTCTC 2 cut(s) 197, 285
BfmI CTRYAG 1 cut(s) 54
BisI GCNGC 4 cut(s) 15, 120, 239, 285
BlsI GCNGC 4 cut(s) 16, 121, 240, 286
Bme1390I CCNGG 3 cut(s) 219, 327, 428
BmgT120I GGNCC 1 cut(s) 167
BmrFI CCNGG 3 cut(s) 219, 327, 428
BmsI GCATC 1 cut(s) 271
BpmI CTGGAG 1 cut(s) 30
BpuMI CCSGG 1 cut(s) 428
BsaJI CCNNGG 2 cut(s) 125, 326
BsaWI WCCGGW 1 cut(s) 500
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 372
Bse1I ACTGG 5 cut(s) 13, 85, 356, 486, 486
Bse3DI GCAATG 1 cut(s) 179
BseAI TCCGGA 1 cut(s) 500
BseBI CCWGG 2 cut(s) 219, 327
BseDI CCNNGG 2 cut(s) 125, 326
BseGI GGATG 1 cut(s) 423
BseLI CCNNNNNNNGG 2 cut(s) 165, 372
BseMI GCAATG 1 cut(s) 179
BseMII CTCAG 2 cut(s) 162, 399
BseNI ACTGG 5 cut(s) 13, 85, 356, 486, 486
BseSI GKGCMC 1 cut(s) 438
BseXI GCAGC 3 cut(s) 26, 131, 250
Bsh1236I CGCG 1 cut(s) 416
BshFI GGCC 3 cut(s) 130, 169, 485
BsiHKAI GWGCWC 1 cut(s) 438
BsiSI CCGG 3 cut(s) 288, 427, 501
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 2 cut(s) 165, 372
BsmAI GTCTC 2 cut(s) 197, 285
BsmFI GGGAC 1 cut(s) 142
BsnI GGCC 3 cut(s) 130, 169, 485
Bsp1286I GDGCHC 1 cut(s) 438
Bsp13I TCCGGA 1 cut(s) 500
Bsp143I GATC 2 cut(s) 354, 497
Bsp19I CCATGG 1 cut(s) 125
BspACI CCGC 3 cut(s) 186, 285, 416
BspANI GGCC 3 cut(s) 130, 169, 485
BspCNI CTCAG 2 cut(s) 163, 400
BspEI TCCGGA 1 cut(s) 500
BspFNI CGCG 1 cut(s) 416
BspMAI CTGCAG 1 cut(s) 58
BspPI GGATC 2 cut(s) 362, 492
BsrDI GCAATG 1 cut(s) 179
BsrI ACTGG 5 cut(s) 13, 85, 356, 486, 486
BssECI CCNNGG 2 cut(s) 125, 326
BssMI GATC 2 cut(s) 354, 497
BssT1I CCWWGG 1 cut(s) 125
Bst2UI CCWGG 2 cut(s) 219, 327
BstC8I GCNNGC 3 cut(s) 50, 54, 186
BstDEI CTNAG 4 cut(s) 41, 171, 408, 454
BstDSI CCRYGG 1 cut(s) 125
BstEII GGTNACC 1 cut(s) 321
BstF5I GGATG 1 cut(s) 423
BstFNI CGCG 1 cut(s) 416
BstHHI GCGC 1 cut(s) 416
BstKTI GATC 2 cut(s) 357, 500
BstMAI GTCTC 2 cut(s) 197, 285
BstMBI GATC 2 cut(s) 354, 497
BstNI CCWGG 2 cut(s) 219, 327
BstNSI RCATGY 1 cut(s) 99
BstPI GGTNACC 1 cut(s) 321
BstSCI CCNGG 3 cut(s) 217, 325, 426
BstSFI CTRYAG 1 cut(s) 54
BstSLI GKGCMC 1 cut(s) 438
BstUI CGCG 1 cut(s) 416
BstV1I GCAGC 3 cut(s) 26, 131, 250
BstX2I RGATCY 2 cut(s) 354, 497
BstYI RGATCY 2 cut(s) 354, 497
BsuRI GGCC 3 cut(s) 130, 169, 485
BtgI CCRYGG 1 cut(s) 125
BtsCI GGATG 1 cut(s) 423
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 7 cut(s) 6, 159, 273, 343, 349, 405, 493
Cac8I GCNNGC 3 cut(s) 50, 54, 186
CfoI GCGC 1 cut(s) 416
Cfr13I GGNCC 1 cut(s) 167
CviAII CATG 2 cut(s) 96, 126
CviJI RGCY 8 cut(s) 48, 84, 90, 122, 130, 169, 222, 485
CviKI_1 RGCY 8 cut(s) 48, 84, 90, 122, 130, 169, 222, 485
DdeI CTNAG 4 cut(s) 41, 171, 408, 454
DpnI GATC 2 cut(s) 356, 499
DpnII GATC 2 cut(s) 354, 497
DraI TTTAAA 1 cut(s) 211
EaeI YGGCCR 1 cut(s) 483
Eco130I CCWWGG 1 cut(s) 125
Eco57I CTGAAG 2 cut(s) 70, 297
Eco91I GGTNACC 1 cut(s) 321
EcoO65I GGTNACC 1 cut(s) 321
EcoRII CCWGG 2 cut(s) 217, 325
EcoT14I CCWWGG 1 cut(s) 125
ErhI CCWWGG 1 cut(s) 125
FaeI CATG 2 cut(s) 99, 129
FaiI YATR 4 cut(s) 19, 21, 97, 127
FaqI GGGAC 1 cut(s) 142
FatI CATG 2 cut(s) 95, 125
FauI CCCGC 1 cut(s) 193
FauNDI CATATG 1 cut(s) 19
FblI GTMKAC 1 cut(s) 478
Fnu4HI GCNGC 4 cut(s) 15, 120, 239, 285
FokI GGATG 1 cut(s) 410
Fsp4HI GCNGC 4 cut(s) 15, 120, 239, 285
GlaI GCGC 1 cut(s) 415
GluI GCNGC 4 cut(s) 15, 120, 239, 285
GsuI CTGGAG 1 cut(s) 30
HaeIII GGCC 3 cut(s) 130, 169, 485
HapII CCGG 3 cut(s) 288, 427, 501
HhaI GCGC 1 cut(s) 416
Hin1II CATG 2 cut(s) 99, 129
Hin6I GCGC 1 cut(s) 414
HinP1I GCGC 1 cut(s) 414
HincII GTYRAC 2 cut(s) 26, 479
HindII GTYRAC 2 cut(s) 26, 479
HinfI GANTC 1 cut(s) 391
HpaII CCGG 3 cut(s) 288, 427, 501
HphI GGTGA 2 cut(s) 303, 315
Hpy166II GTNNAC 4 cut(s) 26, 311, 436, 479
Hpy188I TCNGA 1 cut(s) 202
Hpy188III TCNNGA 2 cut(s) 395, 501
Hpy8I GTNNAC 4 cut(s) 26, 311, 436, 479
HpyAV CCTTC 1 cut(s) 141
HpyCH4V TGCA 3 cut(s) 56, 119, 436
HpyF3I CTNAG 4 cut(s) 41, 171, 408, 454
Hsp92II CATG 2 cut(s) 99, 129
HspAI GCGC 1 cut(s) 414
Kpn2I TCCGGA 1 cut(s) 500
Kzo9I GATC 2 cut(s) 354, 497
LmnI GCTCC 2 cut(s) 11, 127
Lsp1109I GCAGC 3 cut(s) 26, 131, 250
LweI GCATC 1 cut(s) 271
MaeIII GTNAC 4 cut(s) 4, 250, 271, 321
MalI GATC 2 cut(s) 356, 499
MboI GATC 2 cut(s) 354, 497
MboII GAAGA 3 cut(s) 290, 400, 472
MflI RGATCY 2 cut(s) 354, 497
MhlI GDGCHC 1 cut(s) 438
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 3 cut(s) 73, 144, 242
MluNI TGGCCA 1 cut(s) 485
MnlI CCTC 5 cut(s) 151, 170, 227, 403, 438
Mox20I TGGCCA 1 cut(s) 485
MroI TCCGGA 1 cut(s) 500
MroXI GAANNNNTTC 1 cut(s) 339
MscI TGGCCA 1 cut(s) 485
MseI TTAA 2 cut(s) 104, 210
Msp20I TGGCCA 1 cut(s) 485
MspI CCGG 3 cut(s) 288, 427, 501
MspR9I CCNGG 3 cut(s) 219, 327, 428
MvaI CCWGG 2 cut(s) 219, 327
MvnI CGCG 1 cut(s) 416
NciI CCSGG 1 cut(s) 428
NcoI CCATGG 1 cut(s) 125
NdeI CATATG 1 cut(s) 19
NdeII GATC 2 cut(s) 354, 497
NlaIII CATG 2 cut(s) 99, 129
NmuCI GTSAC 3 cut(s) 4, 271, 321
NspI RCATGY 1 cut(s) 99
PciI ACATGT 1 cut(s) 95
PdmI GAANNNNTTC 1 cut(s) 339
PfeI GAWTC 1 cut(s) 391
PflMI CCANNNNNTGG 1 cut(s) 372
PkrI GCNGC 4 cut(s) 16, 121, 240, 286
PscI ACATGT 1 cut(s) 95
Psp6I CCWGG 2 cut(s) 217, 325
PspEI GGTNACC 1 cut(s) 321
PspGI CCWGG 2 cut(s) 217, 325
PspPI GGNCC 1 cut(s) 167
PstI CTGCAG 1 cut(s) 58
PsuI RGATCY 2 cut(s) 354, 497
SalI GTCGAC 1 cut(s) 477
SaqAI TTAA 2 cut(s) 104, 210
SatI GCNGC 4 cut(s) 15, 120, 239, 285
Sau3AI GATC 2 cut(s) 354, 497
Sau96I GGNCC 1 cut(s) 167
ScrFI CCNGG 3 cut(s) 219, 327, 428
SduI GDGCHC 1 cut(s) 438
SetI ASST 4 cut(s) 92, 124, 316, 383
SfaNI GCATC 1 cut(s) 271
SfcI CTRYAG 1 cut(s) 54
Sse9I AATT 3 cut(s) 73, 144, 242
SsiI CCGC 3 cut(s) 186, 285, 416
SspI AATATT 1 cut(s) 115
StyD4I CCNGG 3 cut(s) 217, 325, 426
StyI CCWWGG 1 cut(s) 125
TaqI TCGA 1 cut(s) 478
TasI AATT 3 cut(s) 73, 144, 242
TauI GCSGC 1 cut(s) 287
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 2 cut(s) 104, 210
Tru9I TTAA 2 cut(s) 104, 210
TscAI CASTG 7 cut(s) 13, 159, 280, 350, 356, 412, 493
TseFI GTSAC 3 cut(s) 4, 271, 321
TseI GCWGC 3 cut(s) 14, 119, 238
Tsp45I GTSAC 3 cut(s) 4, 271, 321
TspDTI ATGAA 3 cut(s) 123, 138, 252
TspRI CASTG 7 cut(s) 13, 159, 280, 350, 356, 412, 493
Van91I CCANNNNNTGG 1 cut(s) 372
VneI GTGCAC 1 cut(s) 434
XceI RCATGY 1 cut(s) 99
XmiI GTMKAC 1 cut(s) 478
XmnI GAANNNNTTC 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.