Rmu_sc0004096.1_g000017

Alpha-aminoadipic semialdehyde

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004096.1
OG0022382
Physical Location & Seq
Reverse (-)
78865 .. 79517
653 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 435 bp
atgcttggaaatggggacgttgttgggatcgtctcggagactatgaacaagtgggaaagaagagcgccattgaccccctctcactgtgcacggctgcttcacagtggcagtgacaaaaccggcgtgtctcggatcatcatgcagccatcgacgaagcacatccatcatgatgcattgtatgaagaagttggatgtgaaatttcagaggacctgtcagaatgtggcctcattttggggatcaaacaacccaagccggagatgattcttccggatagagcttatgcttttttctcacatacccacaagacgccgaaagaaaatatgccattgttggataagattctagcagaaagggtgtctttgtatgattatgagctaattgttggggatcatatatgggaggagactgcttgcattcggcaagtttgctggtag

Protein Analysis

144

Amino Acids

16.34

Weight (kDa)

5.71

Isoelectric Point (pI)

54.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AlaDh_PNT_N PF05222 9 - 129 3.5e-17 Alanine dehydrogenase/PNT, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022382)

Species Orthologous Gene IDs
malus_domestica MD02G1105200.v1.1
rosa_multiflora Rmu_sc0004096.1_g000017 Rmu_sc0008498.1_g000001
rosa_samantha Rh2AG104900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 268
AclWI GGATC 4 cut(s) 35, 140, 245, 396
AcsI RAATTY 1 cut(s) 198
AcyI GRCGYC 1 cut(s) 308
AfiI CCNNNNNNNGG 1 cut(s) 232
AluBI AGCT 2 cut(s) 278, 376
AluI AGCT 2 cut(s) 278, 376
Alw21I GWGCWC 1 cut(s) 91
Alw26I GTCTC 4 cut(s) 32, 37, 132, 398
Alw44I GTGCAC 1 cut(s) 87
AlwI GGATC 4 cut(s) 35, 140, 245, 396
Aor13HI TCCGGA 1 cut(s) 268
AoxI GGCC 1 cut(s) 223
ApaLI GTGCAC 1 cut(s) 87
ApeKI GCWGC 2 cut(s) 94, 142
ApoI RAATTY 1 cut(s) 198
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 1 cut(s) 208
AvaII GGWCC 1 cut(s) 208
BaeGI GKGCMC 1 cut(s) 91
Bbv12I GWGCWC 1 cut(s) 91
BbvI GCAGC 2 cut(s) 81, 154
BccI CCATC 2 cut(s) 154, 171
BceAI ACGGC 1 cut(s) 107
BcoDI GTCTC 4 cut(s) 32, 37, 132, 398
BfaI CTAG 1 cut(s) 344
BfoI RGCGCY 1 cut(s) 68
BisI GCNGC 2 cut(s) 95, 143
BlsI GCNGC 2 cut(s) 96, 144
Bme18I GGWCC 1 cut(s) 208
BmgT120I GGNCC 1 cut(s) 208
BmsI GCATC 1 cut(s) 160
BsaHI GRCGYC 1 cut(s) 308
BsaWI WCCGGW 1 cut(s) 268
Bsc4I CCNNNNNNNGG 1 cut(s) 232
Bse118I RCCGGY 1 cut(s) 119
BseAI TCCGGA 1 cut(s) 268
BseGI GGATG 2 cut(s) 159, 197
BseLI CCNNNNNNNGG 1 cut(s) 232
BseRI GAGGAG 1 cut(s) 416
BseSI GKGCMC 1 cut(s) 91
BseXI GCAGC 2 cut(s) 81, 154
BshFI GGCC 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 91
BsiSI CCGG 3 cut(s) 120, 254, 269
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 1 cut(s) 232
BsmAI GTCTC 4 cut(s) 32, 37, 132, 398
BsmBI CGTCTC 1 cut(s) 37
BsmFI GGGAC 1 cut(s) 29
BsmI GAATGC 1 cut(s) 414
BsnI GGCC 1 cut(s) 225
Bsp1286I GDGCHC 1 cut(s) 91
Bsp13I TCCGGA 1 cut(s) 268
Bsp143I GATC 4 cut(s) 27, 132, 237, 388
BspANI GGCC 1 cut(s) 225
BspEI TCCGGA 1 cut(s) 268
BspHI TCATGA 1 cut(s) 166
BspPI GGATC 4 cut(s) 35, 140, 245, 396
BspQI GCTCTTC 1 cut(s) 55
BsrFI RCCGGY 1 cut(s) 119
BssAI RCCGGY 1 cut(s) 119
BssMI GATC 4 cut(s) 27, 132, 237, 388
BssNI GRCGYC 1 cut(s) 308
Bst4CI ACNGT 2 cut(s) 86, 104
Bst6I CTCTTC 1 cut(s) 55
BstACI GRCGYC 1 cut(s) 308
BstC8I GCNNGC 1 cut(s) 412
BstF5I GGATG 2 cut(s) 159, 197
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 4 cut(s) 30, 135, 240, 391
BstMAI GTCTC 4 cut(s) 32, 37, 132, 398
BstMBI GATC 4 cut(s) 27, 132, 237, 388
BstSLI GKGCMC 1 cut(s) 91
BstV1I GCAGC 2 cut(s) 81, 154
BsuRI GGCC 1 cut(s) 225
BtsCI GGATG 2 cut(s) 159, 197
BtsI GCAGTG 1 cut(s) 115
BtsIMutI CAGTG 3 cut(s) 82, 109, 115
Cac8I GCNNGC 1 cut(s) 412
CciI TCATGA 1 cut(s) 166
CfoI GCGC 1 cut(s) 67
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 1 cut(s) 208
CseI GACGC 1 cut(s) 316
CviAII CATG 2 cut(s) 139, 167
CviJI RGCY 6 cut(s) 94, 145, 225, 253, 278, 376
CviKI_1 RGCY 6 cut(s) 94, 145, 225, 253, 278, 376
DpnI GATC 4 cut(s) 29, 134, 239, 390
DpnII GATC 4 cut(s) 27, 132, 237, 388
Eam1104I CTCTTC 1 cut(s) 55
EarI CTCTTC 1 cut(s) 55
Eco47I GGWCC 1 cut(s) 208
EcoO109I RGGNCCY 1 cut(s) 208
EcoT22I ATGCAT 1 cut(s) 175
Esp3I CGTCTC 1 cut(s) 37
FaeI CATG 2 cut(s) 142, 170
FalI AAGNNNNNCTT 2 cut(s) 343, 375
FaqI GGGAC 1 cut(s) 29
FatI CATG 2 cut(s) 138, 166
Fnu4HI GCNGC 2 cut(s) 95, 143
FokI GGATG 2 cut(s) 146, 204
Fsp4HI GCNGC 2 cut(s) 95, 143
FspBI CTAG 1 cut(s) 344
GlaI GCGC 1 cut(s) 66
GluI GCNGC 2 cut(s) 95, 143
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 1 cut(s) 225
HapII CCGG 3 cut(s) 120, 254, 269
HgaI GACGC 1 cut(s) 316
HhaI GCGC 1 cut(s) 67
Hin1I GRCGYC 1 cut(s) 308
Hin1II CATG 2 cut(s) 142, 170
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HinfI GANTC 2 cut(s) 262, 340
HpaII CCGG 3 cut(s) 120, 254, 269
Hpy166II GTNNAC 1 cut(s) 89
Hpy188I TCNGA 4 cut(s) 37, 132, 205, 217
Hpy188III TCNNGA 2 cut(s) 167, 269
Hpy8I GTNNAC 1 cut(s) 89
Hpy99I CGWCG 1 cut(s) 154
HpyCH4III ACNGT 2 cut(s) 86, 104
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 4 cut(s) 89, 142, 173, 414
HpySE526I ACGT 1 cut(s) 18
Hsp92I GRCGYC 1 cut(s) 308
Hsp92II CATG 2 cut(s) 142, 170
HspAI GCGC 1 cut(s) 65
Kpn2I TCCGGA 1 cut(s) 268
Kzo9I GATC 4 cut(s) 27, 132, 237, 388
LguI GCTCTTC 1 cut(s) 55
LpnPI CCDG 5 cut(s) 133, 224, 267, 282, 415
Lsp1109I GCAGC 2 cut(s) 81, 154
LweI GCATC 1 cut(s) 160
MaeI CTAG 1 cut(s) 344
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 1 cut(s) 110
MalI GATC 4 cut(s) 29, 134, 239, 390
MboI GATC 4 cut(s) 27, 132, 237, 388
MboII GAAGA 3 cut(s) 72, 194, 257
MhlI GDGCHC 1 cut(s) 91
MluCI AATT 2 cut(s) 198, 378
MmeI TCCRAC 2 cut(s) 169, 312
MnlI CCTC 4 cut(s) 88, 199, 236, 394
Mph1103I ATGCAT 1 cut(s) 175
MroI TCCGGA 1 cut(s) 268
MslI CAYNNNNRTG 1 cut(s) 168
MspI CCGG 3 cut(s) 120, 254, 269
Mva1269I GAATGC 1 cut(s) 414
NdeII GATC 4 cut(s) 27, 132, 237, 388
NlaIII CATG 2 cut(s) 142, 170
NmuCI GTSAC 1 cut(s) 110
NsiI ATGCAT 1 cut(s) 175
PagI TCATGA 1 cut(s) 166
PciSI GCTCTTC 1 cut(s) 55
PctI GAATGC 1 cut(s) 414
PfeI GAWTC 2 cut(s) 262, 340
PkrI GCNGC 2 cut(s) 96, 144
PpuMI RGGWCCY 1 cut(s) 208
Psp5II RGGWCCY 1 cut(s) 208
PspPI GGNCC 1 cut(s) 208
PspPPI RGGWCCY 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 168
SapI GCTCTTC 1 cut(s) 55
SatI GCNGC 2 cut(s) 95, 143
Sau3AI GATC 4 cut(s) 27, 132, 237, 388
Sau96I GGNCC 1 cut(s) 208
SduI GDGCHC 1 cut(s) 91
SetI ASST 4 cut(s) 21, 213, 280, 378
SfaNI GCATC 1 cut(s) 160
SinI GGWCC 1 cut(s) 208
SmiMI CAYNNNNRTG 1 cut(s) 168
Sse9I AATT 2 cut(s) 198, 378
SspMI CTAG 1 cut(s) 344
TaaI ACNGT 2 cut(s) 86, 104
TaiI ACGT 1 cut(s) 21
TaqI TCGA 1 cut(s) 149
TasI AATT 2 cut(s) 198, 378
TfiI GAWTC 2 cut(s) 262, 340
TscAI CASTG 3 cut(s) 89, 109, 115
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 2 cut(s) 94, 142
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 2 cut(s) 59, 195
TspRI CASTG 3 cut(s) 89, 109, 115
VneI GTGCAC 1 cut(s) 87
VpaK11BI GGWCC 1 cut(s) 208
XapI RAATTY 1 cut(s) 198
XspI CTAG 1 cut(s) 344
Zsp2I ATGCAT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.