Rmu_sc0004549.1_g000020

TPR and ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004549.1
OG0025584
Physical Location & Seq
Forward (+)
96857 .. 98530
1674 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004549.1_g000020.1.cds

Sequence Viewer

Length: 1029 bp
atggtagacgtaaagaaaatgttggaccaatttcatgagttgcccaatgccaacagcataattttcaaaaacgctagatgcaaggtgctttttagtattaactttttgaagttattcaagaatctgatgtggcctatttggagaacgcctcgactgtcgaacccactcacgcgtccgccaacctgcgcccttgtcaacccatcttacgcttatgctaacttgtctagttttctttgtttagatttcgttcctggtaacaaagccagtcgcgaagctttcagcagggctatggataaagccgagttgcaaaacttcatggagggaatgtatgccgctggtcttgaagcgttgaaggctgtcgttgaccagcagtctcaggcactaagccaagcagagacagaggtcgtgatgctgatgccgcatcactcccatctcgccgctgctgggcaaaccattatgttacccaaggccctcgccgagcgagggaccggcggagggggcaggaccagtgtttccctagagaaaggcagggaaagggagcccgctcagcgggagaggacactgccagtgaaggagtctgttactattgcgggcttggagcctcagaaccctccgaggtctgccaatgtaggtgttgcacaaggcgctgtgctaaggaagaggcggcaaaatgatgccatggaagaagatgaggtaggaagttctgacgagtggctagacttgtttgattcatatgctgaattcttgactgagcccgagtgggagtttcttttccacctctacgagcggctggggtttggatggatggatgggacggtatgtccaaaagccctccaacagtcgccgttcaactcggcctttggccatctgtactacgccatgggccgggttcacacctcaccatggcgggctccacataaggtgccaccccgagcacccagagcccggggcaaggccagcacagcccatctattctacaaatacaagactgatagcatcagaagaacctgtgtcccacatcgaggatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003678 GO:0003724 GO:0003824 GO:0004003 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006283 GO:0006289 GO:0006351 GO:0006353 GO:0006355 GO:0006357 GO:0006364 GO:0006366 GO:0006369 GO:0006378 GO:0006396 GO:0006397 GO:0006399 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008033 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016073 GO:0016074 GO:0016180 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019725 GO:0019904 GO:0022613 GO:0031123 GO:0031124 GO:0031126 GO:0031323 GO:0031326 GO:0032392 GO:0032508 GO:0032574 GO:0032575 GO:0032774 GO:0032991 GO:0033554 GO:0033677 GO:0033678 GO:0033680 GO:0033682 GO:0034470 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035649 GO:0042254 GO:0042592 GO:0042623 GO:0043139 GO:0043141 GO:0043144 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043618 GO:0043620 GO:0043628 GO:0043631 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045005 GO:0045454 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:1990248 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12 AAA_19 UvrD-helicase

Protein Analysis

342

Amino Acids

38.44

Weight (kDa)

9.22

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025584)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0004549.1_g000020 Rmu_sc0035593.1_g000001
rosa_roxburghii Rroxscaffold_7G00197040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 191
AccB1I GGYRCC 1 cut(s) 922
AccBSI CCGCTC 2 cut(s) 545, 787
AccI GTMKAC 1 cut(s) 6
AccII CGCG 2 cut(s) 172, 270
AcoI YGGCCR 1 cut(s) 862
AcsI RAATTY 1 cut(s) 740
AfaI GTAC 1 cut(s) 872
AfiI CCNNNNNNNGG 9 cut(s) 444, 483, 495, 549, 885, 903, 945, 952, 1022
AflIII ACRYGT 1 cut(s) 170
AgsI TTSAA 6 cut(s) 67, 109, 118, 344, 352, 850
AhdI GACNNNNNGTC 2 cut(s) 370, 819
AjnI CCWGG 1 cut(s) 250
AluBI AGCT 1 cut(s) 275
AluI AGCT 1 cut(s) 275
Alw21I GWGCWC 1 cut(s) 937
Alw26I GTCTC 2 cut(s) 378, 389
Ama87I CYCGRG 3 cut(s) 755, 930, 945
AoxI GGCC 6 cut(s) 131, 468, 855, 862, 883, 954
ApeKI GCWGC 1 cut(s) 440
ApoI RAATTY 1 cut(s) 740
Asp700I GAANNNNTTC 1 cut(s) 113
AspLEI GCGC 2 cut(s) 188, 647
AspS9I GGNCC 5 cut(s) 25, 469, 486, 504, 883
AsuC2I CCSGG 3 cut(s) 887, 946, 947
AsuHPI GGTGA 1 cut(s) 891
AvaI CYCGRG 3 cut(s) 755, 930, 945
AvaII GGWCC 3 cut(s) 25, 486, 504
BalI TGGCCA 1 cut(s) 864
BanI GGYRCC 1 cut(s) 922
BanII GRGCYC 4 cut(s) 543, 756, 913, 946
Bbv12I GWGCWC 1 cut(s) 937
BbvI GCAGC 1 cut(s) 427
BccI CCATC 7 cut(s) 208, 438, 795, 799, 803, 873, 975
BceAI ACGGC 1 cut(s) 829
BciT130I CCWGG 1 cut(s) 252
BcnI CCSGG 3 cut(s) 887, 946, 947
BcoDI GTCTC 2 cut(s) 378, 389
BfaI CTAG 4 cut(s) 75, 225, 518, 716
BfoI RGCGCY 1 cut(s) 648
BfuAI ACCTGC 1 cut(s) 191
BisI GCNGC 6 cut(s) 333, 419, 438, 441, 665, 788
BlpI GCTNAGC 1 cut(s) 546
BlsI GCNGC 6 cut(s) 334, 420, 439, 442, 666, 789
Bme1390I CCNGG 4 cut(s) 252, 887, 946, 947
Bme18I GGWCC 3 cut(s) 25, 486, 504
BmeRI GACNNNNNGTC 2 cut(s) 370, 819
BmeT110I CYCGRG 3 cut(s) 755, 930, 945
BmgT120I GGNCC 5 cut(s) 25, 469, 486, 504, 883
BmiI GGNNCC 5 cut(s) 487, 540, 600, 912, 924
BmrFI CCNGG 4 cut(s) 252, 887, 946, 947
BmsI GCATC 6 cut(s) 68, 399, 405, 430, 664, 1005
BoxI GACNNNNGTC 1 cut(s) 401
BplI GAGNNNNNCTC 2 cut(s) 133, 165
Bpu10I CCTNAGC 1 cut(s) 653
Bpu1102I GCTNAGC 1 cut(s) 546
BpuMI CCSGG 3 cut(s) 887, 946, 947
BsaJI CCNNGG 7 cut(s) 465, 614, 678, 879, 902, 945, 946
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bsc4I CCNNNNNNNGG 9 cut(s) 444, 483, 495, 549, 885, 903, 945, 952, 1022
Bse118I RCCGGY 1 cut(s) 488
Bse1I ACTGG 3 cut(s) 264, 507, 566
BseBI CCWGG 1 cut(s) 252
BseDI CCNNGG 7 cut(s) 465, 614, 678, 879, 902, 945, 946
BseGI GGATG 3 cut(s) 806, 810, 814
BseLI CCNNNNNNNGG 9 cut(s) 444, 483, 495, 549, 885, 903, 945, 952, 1022
BseMII CTCAG 4 cut(s) 389, 560, 617, 741
BseNI ACTGG 3 cut(s) 264, 507, 566
BseXI GCAGC 1 cut(s) 427
BseYI CCCAGC 2 cut(s) 443, 790
Bsh1236I CGCG 2 cut(s) 172, 270
BshFI GGCC 6 cut(s) 133, 470, 857, 864, 885, 956
BshNI GGYRCC 1 cut(s) 922
BsiHKAI GWGCWC 1 cut(s) 937
BsiHKCI CYCGRG 3 cut(s) 755, 930, 945
BsiSI CCGG 3 cut(s) 489, 886, 946
BslFI GGGAC 3 cut(s) 499, 826, 998
BslI CCNNNNNNNGG 9 cut(s) 444, 483, 495, 549, 885, 903, 945, 952, 1022
BsmAI GTCTC 2 cut(s) 378, 389
BsmFI GGGAC 3 cut(s) 499, 826, 998
BsnI GGCC 6 cut(s) 133, 470, 857, 864, 885, 956
BsoBI CYCGRG 3 cut(s) 755, 930, 945
Bsp1286I GDGCHC 5 cut(s) 543, 756, 913, 937, 946
Bsp1720I GCTNAGC 1 cut(s) 546
Bsp19I CCATGG 3 cut(s) 678, 879, 902
Bsp68I TCGCGA 1 cut(s) 270
BspANI GGCC 6 cut(s) 133, 470, 857, 864, 885, 956
BspCNI CTCAG 4 cut(s) 388, 559, 616, 742
BspFNI CGCG 2 cut(s) 172, 270
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 5 cut(s) 487, 540, 600, 912, 924
BspMI ACCTGC 1 cut(s) 191
BspT107I GGYRCC 1 cut(s) 922
BsrBI CCGCTC 2 cut(s) 545, 787
BsrFI RCCGGY 1 cut(s) 488
BsrI ACTGG 3 cut(s) 264, 507, 566
BssAI RCCGGY 1 cut(s) 488
BssECI CCNNGG 7 cut(s) 465, 614, 678, 879, 902, 945, 946
BssT1I CCWWGG 4 cut(s) 465, 678, 879, 902
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 3 cut(s) 156, 817, 840
Bst6I CTCTTC 1 cut(s) 653
BstC8I GCNNGC 4 cut(s) 543, 592, 909, 958
BstDEI CTNAG 6 cut(s) 375, 383, 546, 603, 653, 750
BstDSI CCRYGG 3 cut(s) 678, 879, 902
BstF5I GGATG 3 cut(s) 806, 810, 814
BstFNI CGCG 2 cut(s) 172, 270
BstH2I RGCGCY 1 cut(s) 648
BstHHI GCGC 2 cut(s) 188, 647
BstMAI GTCTC 2 cut(s) 378, 389
BstMWI GCNNNNNNNGC 8 cut(s) 353, 418, 498, 547, 644, 941, 957, 962
BstNI CCWGG 1 cut(s) 252
BstPAI GACNNNNGTC 1 cut(s) 401
BstSCI CCNGG 4 cut(s) 250, 885, 944, 945
BstUI CGCG 2 cut(s) 172, 270
BstV1I GCAGC 1 cut(s) 427
BsuRI GGCC 6 cut(s) 133, 470, 857, 864, 885, 956
BtgI CCRYGG 3 cut(s) 678, 879, 902
BtsCI GGATG 3 cut(s) 806, 810, 814
BtsI GCAGTG 1 cut(s) 560
BtsIMutI CAGTG 3 cut(s) 514, 560, 573
BtuMI TCGCGA 1 cut(s) 270
BveI ACCTGC 1 cut(s) 191
Cac8I GCNNGC 4 cut(s) 543, 592, 909, 958
CciI TCATGA 1 cut(s) 34
CfoI GCGC 2 cut(s) 188, 647
Cfr10I RCCGGY 1 cut(s) 488
Cfr13I GGNCC 5 cut(s) 25, 469, 486, 504, 883
Cfr9I CCCGGG 1 cut(s) 945
CseI GACGC 1 cut(s) 161
Csp6I GTAC 1 cut(s) 871
CviAII CATG 5 cut(s) 35, 316, 679, 880, 903
CviQI GTAC 1 cut(s) 871
DdeI CTNAG 6 cut(s) 375, 383, 546, 603, 653, 750
DriI GACNNNNNGTC 2 cut(s) 370, 819
EaeI YGGCCR 1 cut(s) 862
Eam1104I CTCTTC 1 cut(s) 653
Eam1105I GACNNNNNGTC 2 cut(s) 370, 819
EarI CTCTTC 1 cut(s) 653
EciI GGCGGA 2 cut(s) 165, 507
Eco130I CCWWGG 4 cut(s) 465, 678, 879, 902
Eco24I GRGCYC 4 cut(s) 543, 756, 913, 946
Eco47I GGWCC 3 cut(s) 25, 486, 504
Eco88I CYCGRG 3 cut(s) 755, 930, 945
EcoO109I RGGNCCY 1 cut(s) 469
EcoRI GAATTC 1 cut(s) 740
EcoRII CCWGG 1 cut(s) 250
EcoT14I CCWWGG 4 cut(s) 465, 678, 879, 902
EcoT38I GRGCYC 4 cut(s) 543, 756, 913, 946
ErhI CCWWGG 4 cut(s) 465, 678, 879, 902
FaeI CATG 5 cut(s) 38, 319, 682, 883, 906
FaqI GGGAC 3 cut(s) 499, 826, 998
FatI CATG 5 cut(s) 34, 315, 678, 879, 902
FauI CCCGC 4 cut(s) 543, 550, 583, 900
FauNDI CATATG 1 cut(s) 733
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 6 cut(s) 333, 419, 438, 441, 665, 788
FokI GGATG 3 cut(s) 813, 817, 821
FriOI GRGCYC 4 cut(s) 543, 756, 913, 946
Fsp4HI GCNGC 6 cut(s) 333, 419, 438, 441, 665, 788
FspBI CTAG 4 cut(s) 75, 225, 518, 716
GlaI GCGC 2 cut(s) 187, 646
GluI GCNGC 6 cut(s) 333, 419, 438, 441, 665, 788
GsaI CCCAGC 2 cut(s) 447, 794
HaeII RGCGCY 1 cut(s) 648
HaeIII GGCC 6 cut(s) 133, 470, 857, 864, 885, 956
HapII CCGG 3 cut(s) 489, 886, 946
HgaI GACGC 1 cut(s) 161
HhaI GCGC 2 cut(s) 188, 647
Hin1II CATG 5 cut(s) 38, 319, 682, 883, 906
Hin6I GCGC 2 cut(s) 186, 645
HinP1I GCGC 2 cut(s) 186, 645
HincII GTYRAC 2 cut(s) 196, 364
HindII GTYRAC 2 cut(s) 196, 364
HindIII AAGCTT 1 cut(s) 273
HinfI GANTC 3 cut(s) 121, 575, 728
HpaII CCGG 3 cut(s) 489, 886, 946
HphI GGTGA 1 cut(s) 891
Hpy166II GTNNAC 4 cut(s) 7, 196, 364, 892
Hpy188I TCNGA 5 cut(s) 126, 606, 615, 706, 1001
Hpy188III TCNNGA 6 cut(s) 35, 118, 269, 341, 406, 745
Hpy8I GTNNAC 4 cut(s) 7, 196, 364, 892
HpyAV CCTTC 2 cut(s) 346, 565
HpyCH4III ACNGT 3 cut(s) 156, 817, 840
HpyCH4IV ACGT 1 cut(s) 9
HpyCH4V TGCA 3 cut(s) 81, 307, 638
HpyF10VI GCNNNNNNNGC 8 cut(s) 353, 418, 498, 547, 644, 941, 957, 962
HpyF3I CTNAG 6 cut(s) 375, 383, 546, 603, 653, 750
HpySE526I ACGT 1 cut(s) 9
Hsp92II CATG 5 cut(s) 38, 319, 682, 883, 906
HspAI GCGC 2 cut(s) 186, 645
LmnI GCTCC 3 cut(s) 538, 598, 916
Lsp1109I GCAGC 1 cut(s) 427
LweI GCATC 6 cut(s) 68, 399, 405, 430, 664, 1005
MaeI CTAG 4 cut(s) 75, 225, 518, 716
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 3 cut(s) 254, 459, 580
MbiI CCGCTC 2 cut(s) 545, 787
MboII GAAGA 4 cut(s) 670, 695, 698, 1014
MhlI GDGCHC 5 cut(s) 543, 756, 913, 937, 946
MlsI TGGCCA 1 cut(s) 864
MluCI AATT 3 cut(s) 29, 60, 740
MluI ACGCGT 1 cut(s) 170
MluNI TGGCCA 1 cut(s) 864
MlyI GAGTC 1 cut(s) 584
MmeI TCCRAC 1 cut(s) 859
Mox20I TGGCCA 1 cut(s) 864
MroXI GAANNNNTTC 1 cut(s) 113
MscI TGGCCA 1 cut(s) 864
MseI TTAA 1 cut(s) 99
Msp20I TGGCCA 1 cut(s) 864
MspA1I CMGCKG 3 cut(s) 335, 440, 550
MspI CCGG 3 cut(s) 489, 886, 946
MspR9I CCNGG 4 cut(s) 252, 887, 946, 947
MvaI CCWGG 1 cut(s) 252
MvnI CGCG 2 cut(s) 172, 270
MwoI GCNNNNNNNGC 8 cut(s) 353, 418, 498, 547, 644, 941, 957, 962
NciI CCSGG 3 cut(s) 887, 946, 947
NcoI CCATGG 3 cut(s) 678, 879, 902
NdeI CATATG 1 cut(s) 733
NlaIII CATG 5 cut(s) 38, 319, 682, 883, 906
NlaIV GGNNCC 5 cut(s) 487, 540, 600, 912, 924
NmeAIII GCCGAG 3 cut(s) 325, 502, 833
NruI TCGCGA 1 cut(s) 270
PagI TCATGA 1 cut(s) 34
PdmI GAANNNNTTC 1 cut(s) 113
PfeI GAWTC 2 cut(s) 121, 728
PkrI GCNGC 6 cut(s) 334, 420, 439, 442, 666, 789
PleI GAGTC 1 cut(s) 583
PpsI GAGTC 1 cut(s) 583
PshAI GACNNNNGTC 1 cut(s) 401
Psp6I CCWGG 1 cut(s) 250
PspFI CCCAGC 2 cut(s) 443, 790
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 5 cut(s) 487, 540, 600, 912, 924
PspPI GGNCC 5 cut(s) 25, 469, 486, 504, 883
RruI TCGCGA 1 cut(s) 270
RsaI GTAC 1 cut(s) 872
RsaNI GTAC 1 cut(s) 871
SaqAI TTAA 1 cut(s) 99
SatI GCNGC 6 cut(s) 333, 419, 438, 441, 665, 788
Sau96I GGNCC 5 cut(s) 25, 469, 486, 504, 883
SchI GAGTC 1 cut(s) 584
ScrFI CCNGG 4 cut(s) 252, 887, 946, 947
SduI GDGCHC 5 cut(s) 543, 756, 913, 937, 946
SfaNI GCATC 6 cut(s) 68, 399, 405, 430, 664, 1005
SinI GGWCC 3 cut(s) 25, 486, 504
SmaI CCCGGG 1 cut(s) 947
Sse9I AATT 3 cut(s) 29, 60, 740
SspMI CTAG 4 cut(s) 75, 225, 518, 716
StyD4I CCNGG 4 cut(s) 250, 885, 944, 945
StyI CCWWGG 4 cut(s) 465, 678, 879, 902
TaaI ACNGT 3 cut(s) 156, 817, 840
TaiI ACGT 1 cut(s) 12
TaqI TCGA 3 cut(s) 151, 158, 1021
TasI AATT 3 cut(s) 29, 60, 740
TatI WGTACW 1 cut(s) 870
TauI GCSGC 5 cut(s) 335, 421, 440, 667, 790
TfiI GAWTC 2 cut(s) 121, 728
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TscAI CASTG 3 cut(s) 514, 567, 573
TseI GCWGC 1 cut(s) 440
TspDTI ATGAA 3 cut(s) 23, 304, 720
TspMI CCCGGG 1 cut(s) 945
TspRI CASTG 3 cut(s) 514, 567, 573
VpaK11BI GGWCC 3 cut(s) 25, 486, 504
XapI RAATTY 1 cut(s) 740
XmaI CCCGGG 1 cut(s) 945
XmiI GTMKAC 1 cut(s) 6
XmnI GAANNNNTTC 1 cut(s) 113
XspI CTAG 4 cut(s) 75, 225, 518, 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.