Rmu_sc0005942.1_g000004

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005942.1
OG0016749
Physical Location & Seq
Forward (+)
25785 .. 26228
444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005942.1_g000004.1.cds

Sequence Viewer

Length: 444 bp
atggaccgagcacaaccgagctccgactcaccgacgcgagagccgaaatcccctgtggttctctccatcgagtgcctcaagggaaccaataaagcagacgagtggaccgacgacatgctccagaccggagacatagtcgaggagctccgtatcggcacctccgtcaccgtcaaggcgccgttcaagaacggcaggagcggcgtccagaagattctgcacgcttccttcaaggccaaggacacgtcgatccaggtcagagtccggcgcggccgggacaacttcaccgagttgcaggcttgtattgtccccaatgactccgccggaaaaaaggtgtacatgcttcggtccatcacggacccgaactacgccgtcgggttctgcgaccgcacggaagccgactgctttgaactacaaggtaagagacgaacacgactcgatcattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

147

Amino Acids

16.46

Weight (kDa)

8.72

Isoelectric Point (pI)

58.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 155, 175
AccBSI CCGCTC 1 cut(s) 198
AccII CGCG 2 cut(s) 37, 267
AciI CCGC 4 cut(s) 198, 267, 318, 385
AclWI GGATC 1 cut(s) 241
AcoI YGGCCR 1 cut(s) 268
AcyI GRCGYC 2 cut(s) 176, 201
AfaI GTAC 1 cut(s) 335
AflIII ACRYGT 1 cut(s) 240
AgsI TTSAA 3 cut(s) 184, 229, 407
AjiI CACGTC 1 cut(s) 243
AjnI CCWGG 1 cut(s) 249
AluBI AGCT 2 cut(s) 21, 145
AluI AGCT 2 cut(s) 21, 145
Alw21I GWGCWC 3 cut(s) 13, 23, 147
Alw26I GTCTC 2 cut(s) 123, 415
AlwI GGATC 1 cut(s) 241
AoxI GGCC 2 cut(s) 231, 268
AspLEI GCGC 2 cut(s) 178, 267
AspS9I GGNCC 4 cut(s) 4, 105, 345, 355
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 3 cut(s) 21, 157, 274
AvaII GGWCC 4 cut(s) 4, 105, 345, 355
BanI GGYRCC 2 cut(s) 155, 175
BanII GRGCYC 2 cut(s) 23, 147
Bbv12I GWGCWC 3 cut(s) 13, 23, 147
BccI CCATC 2 cut(s) 74, 356
BceAI ACGGC 3 cut(s) 163, 205, 353
BciT130I CCWGG 1 cut(s) 251
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 2 cut(s) 123, 415
BfoI RGCGCY 1 cut(s) 179
BisI GCNGC 2 cut(s) 199, 268
BlsI GCNGC 2 cut(s) 200, 269
Bme1390I CCNGG 2 cut(s) 251, 272
Bme18I GGWCC 4 cut(s) 4, 105, 345, 355
BmgBI CACGTC 1 cut(s) 243
BmgT120I GGNCC 4 cut(s) 4, 105, 345, 355
BmiI GGNNCC 4 cut(s) 85, 157, 177, 357
BmrFI CCNGG 2 cut(s) 251, 272
BpmI CTGGAG 1 cut(s) 104
BpuEI CTTGAG 1 cut(s) 62
BpuMI CCSGG 1 cut(s) 272
BsaHI GRCGYC 2 cut(s) 176, 201
BsaJI CCNNGG 1 cut(s) 234
BsaWI WCCGGW 1 cut(s) 125
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
BseBI CCWGG 1 cut(s) 251
BseDI CCNNGG 1 cut(s) 234
BseRI GAGGAG 1 cut(s) 155
BseX3I CGGCCG 1 cut(s) 268
BsgI GTGCAG 1 cut(s) 200
Bsh1236I CGCG 2 cut(s) 37, 267
Bsh1285I CGRYCG 2 cut(s) 271, 385
BshFI GGCC 2 cut(s) 233, 270
BshNI GGYRCC 2 cut(s) 155, 175
BsiEI CGRYCG 2 cut(s) 271, 385
BsiHKAI GWGCWC 3 cut(s) 13, 23, 147
BsiSI CCGG 4 cut(s) 126, 262, 271, 321
BslFI GGGAC 2 cut(s) 287, 290
BsmAI GTCTC 2 cut(s) 123, 415
BsmBI CGTCTC 1 cut(s) 415
BsmFI GGGAC 2 cut(s) 287, 290
BsnI GGCC 2 cut(s) 233, 270
Bsp1286I GDGCHC 3 cut(s) 13, 23, 147
Bsp1407I TGTACA 1 cut(s) 333
Bsp143I GATC 2 cut(s) 246, 436
BspACI CCGC 4 cut(s) 198, 267, 318, 385
BspANI GGCC 2 cut(s) 233, 270
BspFNI CGCG 2 cut(s) 37, 267
BspLI GGNNCC 4 cut(s) 85, 157, 177, 357
BspPI GGATC 1 cut(s) 241
BspT107I GGYRCC 2 cut(s) 155, 175
BsrBI CCGCTC 1 cut(s) 198
BsrGI TGTACA 1 cut(s) 333
BssECI CCNNGG 1 cut(s) 234
BssMI GATC 2 cut(s) 246, 436
BssNI GRCGYC 2 cut(s) 176, 201
BssT1I CCWWGG 1 cut(s) 234
Bst2UI CCWGG 1 cut(s) 251
Bst4CI ACNGT 1 cut(s) 169
BstACI GRCGYC 2 cut(s) 176, 201
BstAUI TGTACA 1 cut(s) 333
BstC8I GCNNGC 2 cut(s) 219, 294
BstFNI CGCG 2 cut(s) 37, 267
BstH2I RGCGCY 1 cut(s) 179
BstHHI GCGC 2 cut(s) 178, 267
BstKTI GATC 2 cut(s) 249, 439
BstMAI GTCTC 2 cut(s) 123, 415
BstMBI GATC 2 cut(s) 246, 436
BstMCI CGRYCG 2 cut(s) 271, 385
BstMWI GCNNNNNNNGC 1 cut(s) 198
BstNI CCWGG 1 cut(s) 251
BstNSI RCATGY 2 cut(s) 118, 340
BstSCI CCNGG 2 cut(s) 249, 270
BstUI CGCG 2 cut(s) 37, 267
BstZI CGGCCG 1 cut(s) 268
BsuRI GGCC 2 cut(s) 233, 270
BtrI CACGTC 1 cut(s) 243
Cac8I GCNNGC 2 cut(s) 219, 294
CfoI GCGC 2 cut(s) 178, 267
Cfr13I GGNCC 4 cut(s) 4, 105, 345, 355
CseI GACGC 2 cut(s) 43, 190
Csp6I GTAC 1 cut(s) 334
CviAII CATG 2 cut(s) 115, 337
CviJI RGCY 7 cut(s) 21, 43, 145, 233, 270, 296, 395
CviKI_1 RGCY 7 cut(s) 21, 43, 145, 233, 270, 296, 395
CviQI GTAC 1 cut(s) 334
DinI GGCGCC 1 cut(s) 177
DpnI GATC 2 cut(s) 248, 438
DpnII GATC 2 cut(s) 246, 436
EaeI YGGCCR 1 cut(s) 268
EagI CGGCCG 1 cut(s) 268
EciI GGCGGA 1 cut(s) 307
Ecl136II GAGCTC 2 cut(s) 21, 145
EclXI CGGCCG 1 cut(s) 268
Eco130I CCWWGG 1 cut(s) 234
Eco24I GRGCYC 2 cut(s) 23, 147
Eco47I GGWCC 4 cut(s) 4, 105, 345, 355
Eco52I CGGCCG 1 cut(s) 268
Eco53kI GAGCTC 2 cut(s) 21, 145
EcoICRI GAGCTC 2 cut(s) 21, 145
EcoRII CCWGG 1 cut(s) 249
EcoT14I CCWWGG 1 cut(s) 234
EcoT38I GRGCYC 2 cut(s) 23, 147
EgeI GGCGCC 1 cut(s) 177
EheI GGCGCC 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 234
Esp3I CGTCTC 1 cut(s) 415
FaeI CATG 2 cut(s) 118, 340
FaiI YATR 3 cut(s) 116, 134, 338
FaqI GGGAC 2 cut(s) 287, 290
FatI CATG 2 cut(s) 114, 336
Fnu4HI GCNGC 2 cut(s) 199, 268
FriOI GRGCYC 2 cut(s) 23, 147
Fsp4HI GCNGC 2 cut(s) 199, 268
GlaI GCGC 2 cut(s) 177, 266
GluI GCNGC 2 cut(s) 199, 268
GsuI CTGGAG 1 cut(s) 104
HaeII RGCGCY 1 cut(s) 179
HaeIII GGCC 2 cut(s) 233, 270
HapII CCGG 4 cut(s) 126, 262, 271, 321
HgaI GACGC 2 cut(s) 43, 190
HhaI GCGC 2 cut(s) 178, 267
Hin1I GRCGYC 2 cut(s) 176, 201
Hin1II CATG 2 cut(s) 118, 340
Hin6I GCGC 2 cut(s) 176, 265
HinP1I GCGC 2 cut(s) 176, 265
HinfI GANTC 5 cut(s) 26, 211, 258, 314, 432
HpaII CCGG 4 cut(s) 126, 262, 271, 321
HphI GGTGA 3 cut(s) 21, 157, 274
Hpy166II GTNNAC 2 cut(s) 105, 334
Hpy188I TCNGA 2 cut(s) 25, 257
Hpy188III TCNNGA 3 cut(s) 121, 184, 205
Hpy8I GTNNAC 2 cut(s) 105, 334
Hpy99I CGWCG 4 cut(s) 37, 113, 247, 374
HpyAV CCTTC 1 cut(s) 235
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4IV ACGT 1 cut(s) 242
HpyCH4V TGCA 2 cut(s) 217, 292
HpyF10VI GCNNNNNNNGC 1 cut(s) 198
HpySE526I ACGT 1 cut(s) 242
Hsp92I GRCGYC 2 cut(s) 176, 201
Hsp92II CATG 2 cut(s) 118, 340
HspAI GCGC 2 cut(s) 176, 265
KasI GGCGCC 1 cut(s) 175
Kzo9I GATC 2 cut(s) 246, 436
LmnI GCTCC 5 cut(s) 26, 123, 142, 150, 195
MaeII ACGT 1 cut(s) 242
MaeIII GTNAC 1 cut(s) 163
MalI GATC 2 cut(s) 248, 438
MbiI CCGCTC 1 cut(s) 198
MboI GATC 2 cut(s) 246, 436
MboII GAAGA 1 cut(s) 220
MhlI GDGCHC 3 cut(s) 13, 23, 147
Mly113I GGCGCC 1 cut(s) 176
MlyI GAGTC 4 cut(s) 20, 267, 308, 426
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 3 cut(s) 86, 133, 169
MseI TTAA 1 cut(s) 442
MspI CCGG 4 cut(s) 126, 262, 271, 321
MspR9I CCNGG 2 cut(s) 251, 272
MvaI CCWGG 1 cut(s) 251
MvnI CGCG 2 cut(s) 37, 267
MwoI GCNNNNNNNGC 1 cut(s) 198
NarI GGCGCC 1 cut(s) 176
NciI CCSGG 1 cut(s) 272
NdeII GATC 2 cut(s) 246, 436
NlaIII CATG 2 cut(s) 118, 340
NlaIV GGNNCC 4 cut(s) 85, 157, 177, 357
NmuCI GTSAC 1 cut(s) 163
NspI RCATGY 2 cut(s) 118, 340
PcsI WCGNNNNNNNCGW 4 cut(s) 41, 105, 159, 378
PfeI GAWTC 1 cut(s) 211
PflFI GACNNNGTC 1 cut(s) 134
PkrI GCNGC 2 cut(s) 200, 269
PleI GAGTC 4 cut(s) 20, 266, 308, 426
PluTI GGCGCC 1 cut(s) 179
PpsI GAGTC 4 cut(s) 20, 266, 308, 426
Psp124BI GAGCTC 2 cut(s) 23, 147
Psp6I CCWGG 1 cut(s) 249
PspGI CCWGG 1 cut(s) 249
PspN4I GGNNCC 4 cut(s) 85, 157, 177, 357
PspPI GGNCC 4 cut(s) 4, 105, 345, 355
PsyI GACNNNGTC 1 cut(s) 134
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SacI GAGCTC 2 cut(s) 23, 147
SaqAI TTAA 1 cut(s) 442
SatI GCNGC 2 cut(s) 199, 268
Sau3AI GATC 2 cut(s) 246, 436
Sau96I GGNCC 4 cut(s) 4, 105, 345, 355
SchI GAGTC 4 cut(s) 20, 267, 308, 426
ScrFI CCNGG 2 cut(s) 251, 272
SduI GDGCHC 3 cut(s) 13, 23, 147
SetI ASST 7 cut(s) 23, 147, 161, 245, 255, 333, 418
SfoI GGCGCC 1 cut(s) 177
SinI GGWCC 4 cut(s) 4, 105, 345, 355
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
SsiI CCGC 4 cut(s) 198, 267, 318, 385
SspDI GGCGCC 1 cut(s) 175
SstI GAGCTC 2 cut(s) 23, 147
StyD4I CCNGG 2 cut(s) 249, 270
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 1 cut(s) 169
TaiI ACGT 1 cut(s) 245
TaqI TCGA 4 cut(s) 69, 138, 245, 435
TaqII GACCGA 3 cut(s) 21, 122, 333
TatI WGTACW 1 cut(s) 333
TauI GCSGC 2 cut(s) 201, 270
TfiI GAWTC 1 cut(s) 211
Tru1I TTAA 1 cut(s) 442
Tru9I TTAA 1 cut(s) 442
TseFI GTSAC 1 cut(s) 163
Tsp45I GTSAC 1 cut(s) 163
TspGWI ACGGA 4 cut(s) 137, 151, 368, 404
Tth111I GACNNNGTC 1 cut(s) 134
VpaK11BI GGWCC 4 cut(s) 4, 105, 345, 355
XceI RCATGY 2 cut(s) 118, 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.