Rmu_sc0005956.1_g000014

DNA gyrase subunit A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005956.1
OG0020436
Physical Location & Seq
Reverse (-)
89338 .. 92549
3212 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 669 bp
atgtccggcagagtaattgcatgggaaaaatatgcctggttgaaatgggaaatatttctgcaaaaccttagtgattactcagctcgtgcatataaaattcctgagtgcagccgcacagcagctggtacaccactggttcaggtccctggtgatgagctgaaatgggtccgttgctgcataaatgatgaccttgtagcgatggcatccctaaacggaatggtcatcttatgttcgtctgatattattcgagccctaggcaggaacactaggggttctgtagccatgcgacttaaagaaggcgataagatggcaagcgtagacattataccagcagctatgcggaaagacttggagacgatctgttgggtttatgtcgttttcactgctctatttcaattttccgcggaggaccgattggcagcagtttttgtggttggattctctgtggcagaggatggtgaaagtgatgaactagtggtcctagtaagccaaagtggtactgtgaatagaattaaggtgcgggacatctcaatacagtctcgctatgcaagggctgttattttgatgcgactcgatcttgctggaaagattcagtctgcttcattaatatcagcaacagatgaggtgccagagattgaagttgatgatgaagctgcaattcatggttaa

Protein Analysis

222

Amino Acids

24.48

Weight (kDa)

4.96

Isoelectric Point (pI)

32.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_gyraseA_C PF03989 63 - 109 5.4e-08 DNA gyrase C-terminal domain, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020436)

Species Orthologous Gene IDs
pyrus_communis pycom07g04210
rosa_chinensis RchiOBHm_Chr1g0331311
rosa_multiflora Rmu_sc0005956.1_g000014 Rmu_sc0005956.1_g000017 Rmu_sc0011261.1_g000004
rosa_samantha Rh1AG117400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 625
AccI GTMKAC 1 cut(s) 318
AccII CGCG 1 cut(s) 404
AciI CCGC 5 cut(s) 112, 340, 402, 404, 520
AcsI RAATTY 1 cut(s) 96
AfaI GTAC 2 cut(s) 127, 499
AfiI CCNNNNNNNGG 1 cut(s) 258
AgsI TTSAA 3 cut(s) 43, 395, 638
AhlI ACTAGT 1 cut(s) 472
AjnI CCWGG 2 cut(s) 35, 145
AloI GAACNNNNNNTCC 2 cut(s) 462, 494
AluBI AGCT 5 cut(s) 83, 122, 157, 335, 653
AluI AGCT 5 cut(s) 83, 122, 157, 335, 653
Alw26I GTCTC 2 cut(s) 347, 543
AlwNI CAGNNNCTG 1 cut(s) 122
ApeKI GCWGC 6 cut(s) 108, 119, 174, 332, 419, 653
ApoI RAATTY 1 cut(s) 96
AseI ATTAAT 1 cut(s) 605
Asp700I GAANNNNTTC 1 cut(s) 54
AspA2I CCTAGG 1 cut(s) 253
AspS9I GGNCC 4 cut(s) 142, 166, 409, 478
AsuHPI GGTGA 2 cut(s) 161, 470
AvaII GGWCC 4 cut(s) 142, 166, 409, 478
AvrII CCTAGG 1 cut(s) 253
BanI GGYRCC 1 cut(s) 625
BanII GRGCYC 1 cut(s) 253
BauI CACGAG 1 cut(s) 84
BbvI GCAGC 6 cut(s) 120, 131, 161, 344, 431, 640
BccI CCATC 3 cut(s) 193, 301, 449
BciT130I CCWGG 2 cut(s) 37, 147
BcoDI GTCTC 2 cut(s) 347, 543
BcuI ACTAGT 1 cut(s) 472
BfaI CTAG 4 cut(s) 254, 267, 473, 482
BfmI CTRYAG 1 cut(s) 276
BisI GCNGC 7 cut(s) 109, 112, 120, 175, 333, 420, 654
BlnI CCTAGG 1 cut(s) 253
BlsI GCNGC 7 cut(s) 110, 113, 121, 176, 334, 421, 655
Bme1390I CCNGG 2 cut(s) 37, 147
Bme18I GGWCC 4 cut(s) 142, 166, 409, 478
BmgT120I GGNCC 4 cut(s) 142, 166, 409, 478
BmiI GGNNCC 3 cut(s) 144, 167, 627
BmrFI CCNGG 2 cut(s) 37, 147
BmsI GCATC 2 cut(s) 212, 555
BsaJI CCNNGG 3 cut(s) 145, 253, 402
Bsc4I CCNNNNNNNGG 1 cut(s) 258
Bse1I ACTGG 1 cut(s) 138
BseBI CCWGG 2 cut(s) 37, 147
BseDI CCNNGG 3 cut(s) 145, 253, 402
BseGI GGATG 2 cut(s) 203, 460
BseLI CCNNNNNNNGG 1 cut(s) 258
BseMII CTCAG 2 cut(s) 93, 93
BseNI ACTGG 1 cut(s) 138
BseXI GCAGC 6 cut(s) 120, 131, 161, 344, 431, 640
BsgI GTGCAG 1 cut(s) 127
Bsh1236I CGCG 1 cut(s) 404
BshNI GGYRCC 1 cut(s) 625
BsiSI CCGG 1 cut(s) 6
BslFI GGGAC 2 cut(s) 128, 536
BslI CCNNNNNNNGG 1 cut(s) 258
BsmAI GTCTC 2 cut(s) 347, 543
BsmBI CGTCTC 1 cut(s) 347
BsmFI GGGAC 2 cut(s) 128, 536
Bsp1286I GDGCHC 1 cut(s) 253
Bsp143I GATC 2 cut(s) 357, 574
BspACI CCGC 5 cut(s) 112, 340, 402, 404, 520
BspCNI CTCAG 2 cut(s) 92, 94
BspFNI CGCG 1 cut(s) 404
BspLI GGNNCC 3 cut(s) 144, 167, 627
BspT107I GGYRCC 1 cut(s) 625
BsrI ACTGG 1 cut(s) 138
BssECI CCNNGG 3 cut(s) 145, 253, 402
BssMI GATC 2 cut(s) 357, 574
BssSI CACGAG 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 253
Bst2BI CACGAG 1 cut(s) 84
Bst2UI CCWGG 2 cut(s) 37, 147
Bst4CI ACNGT 2 cut(s) 502, 537
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 3 cut(s) 68, 79, 102
BstDSI CCRYGG 1 cut(s) 402
BstF5I GGATG 2 cut(s) 203, 460
BstFNI CGCG 1 cut(s) 404
BstKTI GATC 2 cut(s) 360, 577
BstMAI GTCTC 2 cut(s) 347, 543
BstMBI GATC 2 cut(s) 357, 574
BstNI CCWGG 2 cut(s) 37, 147
BstSCI CCNGG 2 cut(s) 35, 145
BstSFI CTRYAG 1 cut(s) 276
BstUI CGCG 1 cut(s) 404
BstV1I GCAGC 6 cut(s) 120, 131, 161, 344, 431, 640
BtgI CCRYGG 1 cut(s) 402
BtgZI GCGATG 1 cut(s) 212
BtsCI GGATG 2 cut(s) 203, 460
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 2 cut(s) 131, 381
Cac8I GCNNGC 1 cut(s) 313
CaiI CAGNNNCTG 1 cut(s) 122
Cfr13I GGNCC 4 cut(s) 142, 166, 409, 478
Cfr42I CCGCGG 1 cut(s) 405
Csp6I GTAC 2 cut(s) 126, 498
CviAII CATG 3 cut(s) 21, 283, 662
CviQI GTAC 2 cut(s) 126, 498
DdeI CTNAG 3 cut(s) 68, 79, 102
DpnI GATC 2 cut(s) 359, 576
DpnII GATC 2 cut(s) 357, 574
Eco130I CCWWGG 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 253
Eco47I GGWCC 4 cut(s) 142, 166, 409, 478
EcoO109I RGGNCCY 1 cut(s) 142
EcoRII CCWGG 2 cut(s) 35, 145
EcoT14I CCWWGG 1 cut(s) 253
EcoT38I GRGCYC 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 253
Esp3I CGTCTC 1 cut(s) 347
FaeI CATG 3 cut(s) 24, 286, 665
FaqI GGGAC 2 cut(s) 128, 536
FatI CATG 3 cut(s) 20, 282, 661
FauI CCCGC 1 cut(s) 513
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 7 cut(s) 109, 112, 120, 175, 333, 420, 654
FokI GGATG 2 cut(s) 190, 467
FriOI GRGCYC 1 cut(s) 253
Fsp4HI GCNGC 7 cut(s) 109, 112, 120, 175, 333, 420, 654
FspBI CTAG 4 cut(s) 254, 267, 473, 482
GluI GCNGC 7 cut(s) 109, 112, 120, 175, 333, 420, 654
HapII CCGG 1 cut(s) 6
Hin1II CATG 3 cut(s) 24, 286, 665
HinfI GANTC 3 cut(s) 438, 570, 589
HpaII CCGG 1 cut(s) 6
HphI GGTGA 2 cut(s) 161, 470
Hpy166II GTNNAC 2 cut(s) 128, 319
Hpy188I TCNGA 1 cut(s) 238
Hpy188III TCNNGA 1 cut(s) 101
Hpy8I GTNNAC 2 cut(s) 128, 319
HpyAV CCTTC 1 cut(s) 290
HpyCH4III ACNGT 2 cut(s) 502, 537
HpyCH4V TGCA 7 cut(s) 20, 61, 89, 108, 177, 548, 656
HpyF3I CTNAG 3 cut(s) 68, 79, 102
Hsp92II CATG 3 cut(s) 24, 286, 665
KspI CCGCGG 1 cut(s) 405
Kzo9I GATC 2 cut(s) 357, 574
Lsp1109I GCAGC 6 cut(s) 120, 131, 161, 344, 431, 640
LweI GCATC 2 cut(s) 212, 555
MaeI CTAG 4 cut(s) 254, 267, 473, 482
MalI GATC 2 cut(s) 359, 576
MboI GATC 2 cut(s) 357, 574
MhlI GDGCHC 1 cut(s) 253
MluCI AATT 5 cut(s) 15, 96, 395, 510, 657
MlyI GAGTC 1 cut(s) 564
MmeI TCCRAC 1 cut(s) 415
MnlI CCTC 3 cut(s) 400, 445, 616
MroXI GAANNNNTTC 1 cut(s) 54
MseI TTAA 4 cut(s) 291, 513, 605, 667
MspA1I CMGCKG 2 cut(s) 122, 404
MspI CCGG 1 cut(s) 6
MspR9I CCNGG 2 cut(s) 37, 147
MvaI CCWGG 2 cut(s) 37, 147
MvnI CGCG 1 cut(s) 404
NdeII GATC 2 cut(s) 357, 574
NlaIII CATG 3 cut(s) 24, 286, 665
NlaIV GGNNCC 3 cut(s) 144, 167, 627
PdmI GAANNNNTTC 1 cut(s) 54
PfeI GAWTC 2 cut(s) 438, 589
PkrI GCNGC 7 cut(s) 110, 113, 121, 176, 334, 421, 655
PleI GAGTC 1 cut(s) 564
PpsI GAGTC 1 cut(s) 564
PpuMI RGGWCCY 1 cut(s) 142
PshBI ATTAAT 1 cut(s) 605
Psp5II RGGWCCY 1 cut(s) 142
Psp6I CCWGG 2 cut(s) 35, 145
PspGI CCWGG 2 cut(s) 35, 145
PspN4I GGNNCC 3 cut(s) 144, 167, 627
PspPI GGNCC 4 cut(s) 142, 166, 409, 478
PspPPI RGGWCCY 1 cut(s) 142
PstNI CAGNNNCTG 1 cut(s) 122
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 2 cut(s) 127, 499
RsaNI GTAC 2 cut(s) 126, 498
SacII CCGCGG 1 cut(s) 405
SaqAI TTAA 4 cut(s) 291, 513, 605, 667
SatI GCNGC 7 cut(s) 109, 112, 120, 175, 333, 420, 654
Sau3AI GATC 2 cut(s) 357, 574
Sau96I GGNCC 4 cut(s) 142, 166, 409, 478
SchI GAGTC 1 cut(s) 564
ScrFI CCNGG 2 cut(s) 37, 147
SduI GDGCHC 1 cut(s) 253
SfaNI GCATC 2 cut(s) 212, 555
SfcI CTRYAG 1 cut(s) 276
Sfr303I CCGCGG 1 cut(s) 405
SgrBI CCGCGG 1 cut(s) 405
SinI GGWCC 4 cut(s) 142, 166, 409, 478
SpeI ACTAGT 1 cut(s) 472
Sse9I AATT 5 cut(s) 15, 96, 395, 510, 657
SsiI CCGC 5 cut(s) 112, 340, 402, 404, 520
SspI AATATT 1 cut(s) 54
SspMI CTAG 4 cut(s) 254, 267, 473, 482
StyD4I CCNGG 2 cut(s) 35, 145
StyI CCWWGG 1 cut(s) 253
TaaI ACNGT 2 cut(s) 502, 537
TaqI TCGA 2 cut(s) 247, 573
TaqII GACCGA 1 cut(s) 426
TasI AATT 5 cut(s) 15, 96, 395, 510, 657
TauI GCSGC 1 cut(s) 114
TfiI GAWTC 2 cut(s) 438, 589
Tru1I TTAA 4 cut(s) 291, 513, 605, 667
Tru9I TTAA 4 cut(s) 291, 513, 605, 667
TscAI CASTG 2 cut(s) 138, 388
TseI GCWGC 6 cut(s) 108, 119, 174, 332, 419, 653
TspDTI ATGAA 4 cut(s) 483, 591, 650, 663
TspGWI ACGGA 2 cut(s) 158, 228
TspRI CASTG 2 cut(s) 138, 388
VpaK11BI GGWCC 4 cut(s) 142, 166, 409, 478
VspI ATTAAT 1 cut(s) 605
XapI RAATTY 1 cut(s) 96
XmaJI CCTAGG 1 cut(s) 253
XmiI GTMKAC 1 cut(s) 318
XmnI GAANNNNTTC 1 cut(s) 54
XspI CTAG 4 cut(s) 254, 267, 473, 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.