Rmu_sc0021221.1_g000001

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0021221.1
OG0016444
Physical Location & Seq
Forward (+)
1 .. 637
637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0021221.1_g000001.1.cds

Sequence Viewer

Length: 637 bp
cgccgacgacgatgcaccccatcccaactcctccgactcatcccccttctgcgccacgtacgaggaaatggtggacagcggcgatccggacttccagtgggccctgccagagaacgagtgggacccgatgacgctgaactacacttccggcacgacttcctcgcccaagggagtggtccactgccacagagggctcttcatcatcaccgtcaactctctcctcgactgggccgtacccagacagcccgtctatctctggacgcttccgatgttccacgccaacggctggagctacccgtacgggatcgccgccgtgggagggaccaacgtctgcgtccgcaaattcgacgcgcctgtcatctacgacttgatcagacgacacggcgtcactcacatgtgcggtgcgccggtggtcctcaacatgctgaccaatgtaccgaacagcgagcctttgaggaacccggtccagatcatgaccgccggagctccgcctcccgcagcggttctgtctcggactgagtcgctgggattcgtggtgagccatgggtacgggttgacggagacggcggggctggtggtgtcgtgcgcgtggaagcccgggtggaataagttgccggcgacggagagggcgaggctg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

212

Amino Acids

23.11

Weight (kDa)

5.56

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 354
AccB7I CCANNNNNTGG 1 cut(s) 286
AccII CGCG 2 cut(s) 351, 588
AccIII TCCGGA 1 cut(s) 86
AciI CCGC 9 cut(s) 79, 310, 338, 400, 478, 489, 496, 501, 567
AclWI GGATC 2 cut(s) 78, 312
AcsI RAATTY 1 cut(s) 342
AcyI GRCGYC 1 cut(s) 385
AfaI GTAC 5 cut(s) 60, 235, 300, 436, 549
AfiI CCNNNNNNNGG 3 cut(s) 227, 286, 319
AflIII ACRYGT 1 cut(s) 394
AhdI GACNNNNNGTC 2 cut(s) 246, 384
AloI GAACNNNNNNTCC 2 cut(s) 129, 161
AluBI AGCT 2 cut(s) 292, 486
AluI AGCT 2 cut(s) 292, 486
Alw21I GWGCWC 1 cut(s) 488
Alw26I GTCTC 2 cut(s) 514, 555
AlwI GGATC 2 cut(s) 78, 312
Ama87I CYCGRG 1 cut(s) 597
Aor13HI TCCGGA 1 cut(s) 86
AoxI GGCC 2 cut(s) 100, 229
ApaI GGGCCC 1 cut(s) 104
ApeKI GCWGC 1 cut(s) 498
ApoI RAATTY 1 cut(s) 342
AspLEI GCGC 4 cut(s) 54, 353, 407, 588
AspS9I GGNCC 8 cut(s) 100, 101, 122, 176, 229, 322, 413, 464
AsuC2I CCSGG 3 cut(s) 462, 598, 599
AsuHPI GGTGA 2 cut(s) 197, 548
AvaI CYCGRG 1 cut(s) 597
AvaII GGWCC 5 cut(s) 122, 176, 322, 413, 464
BaeGI GKGCMC 1 cut(s) 104
BaeI ACNNNNGTAYC 2 cut(s) 418, 451
BanII GRGCYC 3 cut(s) 104, 196, 488
Bbv12I GWGCWC 1 cut(s) 488
BbvI GCAGC 1 cut(s) 510
BccI CCATC 1 cut(s) 28
BceAI ACGGC 5 cut(s) 216, 297, 299, 398, 580
BcgI CGANNNNNNTGC 1 cut(s) 28
BclI TGATCA 1 cut(s) 370
BcnI CCSGG 3 cut(s) 462, 598, 599
BcoDI GTCTC 2 cut(s) 514, 555
BisI GCNGC 3 cut(s) 80, 310, 499
BlsI GCNGC 3 cut(s) 81, 311, 500
Bme1390I CCNGG 3 cut(s) 462, 598, 599
Bme18I GGWCC 5 cut(s) 122, 176, 322, 413, 464
BmeRI GACNNNNNGTC 2 cut(s) 246, 384
BmeT110I CYCGRG 1 cut(s) 597
BmgT120I GGNCC 8 cut(s) 100, 101, 122, 176, 229, 322, 413, 464
BmiI GGNNCC 5 cut(s) 102, 123, 124, 323, 459
BmrFI CCNGG 3 cut(s) 462, 598, 599
BmrI ACTGGG 1 cut(s) 236
BmsI GCATC 1 cut(s) 2
BmuI ACTGGG 1 cut(s) 236
BoxI GACNNNNGTC 1 cut(s) 327
BpmI CTGGAG 1 cut(s) 308
BpuMI CCSGG 3 cut(s) 462, 598, 599
BsaAI YACGTR 1 cut(s) 58
BsaHI GRCGYC 1 cut(s) 385
BsaJI CCNNGG 4 cut(s) 166, 313, 542, 597
BsaWI WCCGGW 1 cut(s) 86
Bsc4I CCNNNNNNNGG 3 cut(s) 227, 286, 319
Bse118I RCCGGY 2 cut(s) 407, 614
Bse1I ACTGG 2 cut(s) 95, 231
BseAI TCCGGA 1 cut(s) 86
BseDI CCNNGG 4 cut(s) 166, 313, 542, 597
BseGI GGATG 2 cut(s) 20, 39
BseLI CCNNNNNNNGG 3 cut(s) 227, 286, 319
BseMII CTCAG 1 cut(s) 508
BseNI ACTGG 2 cut(s) 95, 231
BseRI GAGGAG 2 cut(s) 20, 210
BseSI GKGCMC 1 cut(s) 104
BseXI GCAGC 1 cut(s) 510
BseYI CCCAGC 1 cut(s) 524
Bsh1236I CGCG 2 cut(s) 351, 588
BshFI GGCC 2 cut(s) 102, 231
BsiHKAI GWGCWC 1 cut(s) 488
BsiHKCI CYCGRG 1 cut(s) 597
BsiSI CCGG 7 cut(s) 87, 148, 408, 462, 481, 598, 615
BsiWI CGTACG 2 cut(s) 58, 298
BslFI GGGAC 2 cut(s) 135, 335
BslI CCNNNNNNNGG 3 cut(s) 227, 286, 319
BsmAI GTCTC 2 cut(s) 514, 555
BsmBI CGTCTC 1 cut(s) 555
BsmFI GGGAC 2 cut(s) 135, 335
BsnI GGCC 2 cut(s) 102, 231
BsoBI CYCGRG 1 cut(s) 597
Bsp120I GGGCCC 1 cut(s) 100
Bsp1286I GDGCHC 3 cut(s) 104, 196, 488
Bsp13I TCCGGA 1 cut(s) 86
Bsp143I GATC 4 cut(s) 83, 304, 370, 469
Bsp19I CCATGG 1 cut(s) 542
BspACI CCGC 9 cut(s) 79, 310, 338, 400, 478, 489, 496, 501, 567
BspANI GGCC 2 cut(s) 102, 231
BspCNI CTCAG 1 cut(s) 509
BspEI TCCGGA 1 cut(s) 86
BspFNI CGCG 2 cut(s) 351, 588
BspHI TCATGA 1 cut(s) 472
BspLI GGNNCC 5 cut(s) 102, 123, 124, 323, 459
BspPI GGATC 2 cut(s) 78, 312
BspQI GCTCTTC 1 cut(s) 201
BsrFI RCCGGY 2 cut(s) 407, 614
BsrI ACTGG 2 cut(s) 95, 231
BssAI RCCGGY 2 cut(s) 407, 614
BssECI CCNNGG 4 cut(s) 166, 313, 542, 597
BssMI GATC 4 cut(s) 83, 304, 370, 469
BssNI GRCGYC 1 cut(s) 385
BssT1I CCWWGG 2 cut(s) 166, 542
Bst4CI ACNGT 1 cut(s) 209
Bst6I CTCTTC 1 cut(s) 201
BstACI GRCGYC 1 cut(s) 385
BstBAI YACGTR 1 cut(s) 58
BstC8I GCNNGC 2 cut(s) 447, 616
BstDEI CTNAG 1 cut(s) 517
BstDSI CCRYGG 2 cut(s) 313, 542
BstF5I GGATG 2 cut(s) 20, 39
BstFNI CGCG 2 cut(s) 351, 588
BstHHI GCGC 4 cut(s) 54, 353, 407, 588
BstKTI GATC 4 cut(s) 86, 307, 373, 472
BstMAI GTCTC 2 cut(s) 514, 555
BstMBI GATC 4 cut(s) 83, 304, 370, 469
BstNSI RCATGY 2 cut(s) 398, 425
BstPAI GACNNNNGTC 1 cut(s) 327
BstSCI CCNGG 3 cut(s) 460, 596, 597
BstSLI GKGCMC 1 cut(s) 104
BstUI CGCG 2 cut(s) 351, 588
BstV1I GCAGC 1 cut(s) 510
BstXI CCANNNNNNTGG 1 cut(s) 173
BsuRI GGCC 2 cut(s) 102, 231
BtgI CCRYGG 2 cut(s) 313, 542
BtsCI GGATG 2 cut(s) 20, 39
BtsI GCAGTG 1 cut(s) 179
BtsIMutI CAGTG 2 cut(s) 102, 179
Cac8I GCNNGC 2 cut(s) 447, 616
CciI TCATGA 1 cut(s) 472
CfoI GCGC 4 cut(s) 54, 353, 407, 588
Cfr10I RCCGGY 2 cut(s) 407, 614
Cfr13I GGNCC 8 cut(s) 100, 101, 122, 176, 229, 322, 413, 464
Cfr9I CCCGGG 1 cut(s) 597
CseI GACGC 5 cut(s) 140, 269, 323, 357, 374
Csp6I GTAC 5 cut(s) 59, 234, 299, 435, 548
CviAII CATG 4 cut(s) 395, 422, 473, 543
CviQI GTAC 5 cut(s) 59, 234, 299, 435, 548
DdeI CTNAG 1 cut(s) 517
DpnI GATC 4 cut(s) 85, 306, 372, 471
DpnII GATC 4 cut(s) 83, 304, 370, 469
DrdI GACNNNNNNGTC 1 cut(s) 354
DriI GACNNNNNGTC 2 cut(s) 246, 384
DseDI GACNNNNNNGTC 1 cut(s) 354
Eam1104I CTCTTC 1 cut(s) 201
Eam1105I GACNNNNNGTC 2 cut(s) 246, 384
EarI CTCTTC 1 cut(s) 201
EciI GGCGGA 1 cut(s) 478
Ecl136II GAGCTC 1 cut(s) 486
Eco130I CCWWGG 2 cut(s) 166, 542
Eco24I GRGCYC 3 cut(s) 104, 196, 488
Eco47I GGWCC 5 cut(s) 122, 176, 322, 413, 464
Eco53kI GAGCTC 1 cut(s) 486
Eco88I CYCGRG 1 cut(s) 597
EcoICRI GAGCTC 1 cut(s) 486
EcoO109I RGGNCCY 2 cut(s) 101, 122
EcoT14I CCWWGG 2 cut(s) 166, 542
EcoT38I GRGCYC 3 cut(s) 104, 196, 488
ErhI CCWWGG 2 cut(s) 166, 542
Esp3I CGTCTC 1 cut(s) 555
FaeI CATG 4 cut(s) 398, 425, 476, 546
FaiI YATR 4 cut(s) 396, 423, 474, 544
FaqI GGGAC 2 cut(s) 135, 335
FatI CATG 4 cut(s) 394, 421, 472, 542
FauI CCCGC 2 cut(s) 503, 560
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 3 cut(s) 80, 310, 499
FokI GGATG 2 cut(s) 7, 26
FriOI GRGCYC 3 cut(s) 104, 196, 488
Fsp4HI GCNGC 3 cut(s) 80, 310, 499
GlaI GCGC 4 cut(s) 53, 352, 406, 587
GluI GCNGC 3 cut(s) 80, 310, 499
GsaI CCCAGC 1 cut(s) 528
GsuI CTGGAG 1 cut(s) 308
HaeIII GGCC 2 cut(s) 102, 231
HapII CCGG 7 cut(s) 87, 148, 408, 462, 481, 598, 615
HgaI GACGC 5 cut(s) 140, 269, 323, 357, 374
HhaI GCGC 4 cut(s) 54, 353, 407, 588
Hin1I GRCGYC 1 cut(s) 385
Hin1II CATG 4 cut(s) 398, 425, 476, 546
Hin6I GCGC 4 cut(s) 52, 351, 405, 586
HinP1I GCGC 4 cut(s) 52, 351, 405, 586
HincII GTYRAC 2 cut(s) 212, 556
HindII GTYRAC 2 cut(s) 212, 556
HinfI GANTC 3 cut(s) 36, 519, 529
HpaII CCGG 7 cut(s) 87, 148, 408, 462, 481, 598, 615
HphI GGTGA 2 cut(s) 197, 548
Hpy166II GTNNAC 4 cut(s) 74, 179, 212, 556
Hpy188I TCNGA 4 cut(s) 35, 268, 375, 514
Hpy188III TCNNGA 4 cut(s) 87, 257, 467, 473
Hpy8I GTNNAC 4 cut(s) 74, 179, 212, 556
Hpy99I CGWCG 4 cut(s) 9, 12, 351, 623
HpyAV CCTTC 1 cut(s) 56
HpyCH4III ACNGT 1 cut(s) 209
HpyCH4IV ACGT 2 cut(s) 57, 328
HpyCH4V TGCA 1 cut(s) 15
HpyF3I CTNAG 1 cut(s) 517
HpySE526I ACGT 2 cut(s) 57, 328
Hsp92I GRCGYC 1 cut(s) 385
Hsp92II CATG 4 cut(s) 398, 425, 476, 546
HspAI GCGC 4 cut(s) 52, 351, 405, 586
KflI GGGWCCC 1 cut(s) 122
Kpn2I TCCGGA 1 cut(s) 86
KroI GCCGGC 1 cut(s) 614
KroNI GCCGGC 1 cut(s) 616
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 4 cut(s) 83, 304, 370, 469
LguI GCTCTTC 1 cut(s) 201
LmnI GCTCC 3 cut(s) 289, 483, 491
Lsp1109I GCAGC 1 cut(s) 510
LweI GCATC 1 cut(s) 2
MaeII ACGT 2 cut(s) 57, 328
MaeIII GTNAC 1 cut(s) 386
MalI GATC 4 cut(s) 85, 306, 372, 471
MboI GATC 4 cut(s) 83, 304, 370, 469
MboII GAAGA 1 cut(s) 188
MhlI GDGCHC 3 cut(s) 104, 196, 488
MluCI AATT 1 cut(s) 342
MlyI GAGTC 2 cut(s) 30, 528
MmeI TCCRAC 1 cut(s) 58
MroI TCCGGA 1 cut(s) 86
MroNI GCCGGC 1 cut(s) 614
MslI CAYNNNNRTG 1 cut(s) 393
MspA1I CMGCKG 2 cut(s) 79, 501
MspI CCGG 7 cut(s) 87, 148, 408, 462, 481, 598, 615
MspR9I CCNGG 3 cut(s) 462, 598, 599
MvnI CGCG 2 cut(s) 351, 588
NaeI GCCGGC 1 cut(s) 616
NciI CCSGG 3 cut(s) 462, 598, 599
NcoI CCATGG 1 cut(s) 542
NdeII GATC 4 cut(s) 83, 304, 370, 469
NgoMIV GCCGGC 1 cut(s) 614
NlaIII CATG 4 cut(s) 398, 425, 476, 546
NlaIV GGNNCC 5 cut(s) 102, 123, 124, 323, 459
NmuCI GTSAC 1 cut(s) 386
NspI RCATGY 2 cut(s) 398, 425
PagI TCATGA 1 cut(s) 472
PciI ACATGT 1 cut(s) 394
PciSI GCTCTTC 1 cut(s) 201
PcsI WCGNNNNNNNCGW 2 cut(s) 229, 627
PdiI GCCGGC 1 cut(s) 616
PfeI GAWTC 1 cut(s) 529
Pfl23II CGTACG 2 cut(s) 58, 298
PflFI GACNNNGTC 1 cut(s) 518
PflMI CCANNNNNTGG 1 cut(s) 286
PkrI GCNGC 3 cut(s) 81, 311, 500
PleI GAGTC 2 cut(s) 30, 527
PpsI GAGTC 2 cut(s) 30, 527
Ppu21I YACGTR 1 cut(s) 58
PpuMI RGGWCCY 1 cut(s) 122
PscI ACATGT 1 cut(s) 394
PshAI GACNNNNGTC 1 cut(s) 327
Psp124BI GAGCTC 1 cut(s) 488
Psp5II RGGWCCY 1 cut(s) 122
PspFI CCCAGC 1 cut(s) 524
PspLI CGTACG 2 cut(s) 58, 298
PspN4I GGNNCC 5 cut(s) 102, 123, 124, 323, 459
PspOMI GGGCCC 1 cut(s) 100
PspPI GGNCC 8 cut(s) 100, 101, 122, 176, 229, 322, 413, 464
PspPPI RGGWCCY 1 cut(s) 122
PsyI GACNNNGTC 1 cut(s) 518
RsaI GTAC 5 cut(s) 60, 235, 300, 436, 549
RsaNI GTAC 5 cut(s) 59, 234, 299, 435, 548
RseI CAYNNNNRTG 1 cut(s) 393
SacI GAGCTC 1 cut(s) 488
SapI GCTCTTC 1 cut(s) 201
SatI GCNGC 3 cut(s) 80, 310, 499
Sau3AI GATC 4 cut(s) 83, 304, 370, 469
Sau96I GGNCC 8 cut(s) 100, 101, 122, 176, 229, 322, 413, 464
SchI GAGTC 2 cut(s) 30, 528
ScrFI CCNGG 3 cut(s) 462, 598, 599
SduI GDGCHC 3 cut(s) 104, 196, 488
SetI ASST 4 cut(s) 60, 294, 331, 488
SfaNI GCATC 1 cut(s) 2
SgrAI CRCCGGYG 1 cut(s) 407
SinI GGWCC 5 cut(s) 122, 176, 322, 413, 464
SmaI CCCGGG 1 cut(s) 599
SmiMI CAYNNNNRTG 1 cut(s) 393
Sse9I AATT 1 cut(s) 342
SsiI CCGC 9 cut(s) 79, 310, 338, 400, 478, 489, 496, 501, 567
SstI GAGCTC 1 cut(s) 488
StyD4I CCNGG 3 cut(s) 460, 596, 597
StyI CCWWGG 2 cut(s) 166, 542
TaaI ACNGT 1 cut(s) 209
TaiI ACGT 2 cut(s) 60, 331
TaqI TCGA 2 cut(s) 223, 346
TasI AATT 1 cut(s) 342
TauI GCSGC 2 cut(s) 82, 312
TfiI GAWTC 1 cut(s) 529
TscAI CASTG 2 cut(s) 102, 186
TseFI GTSAC 1 cut(s) 386
TseI GCWGC 1 cut(s) 498
Tsp45I GTSAC 1 cut(s) 386
TspDTI ATGAA 1 cut(s) 188
TspGWI ACGGA 2 cut(s) 573, 636
TspMI CCCGGG 1 cut(s) 597
TspRI CASTG 2 cut(s) 102, 186
Tth111I GACNNNGTC 1 cut(s) 518
Van91I CCANNNNNTGG 1 cut(s) 286
VpaK11BI GGWCC 5 cut(s) 122, 176, 322, 413, 464
XapI RAATTY 1 cut(s) 342
XceI RCATGY 2 cut(s) 398, 425
XcmI CCANNNNNNNNNTGG 1 cut(s) 115
XmaI CCCGGG 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.