Rmu_sc0033786.1_g000001

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0033786.1
OG0016444
Physical Location & Seq
Forward (+)
1 .. 611
611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0033786.1_g000001.1.cds

Sequence Viewer

Length: 611 bp
tcagctccctcgtcctcgacgccctccatctcttccctcccaacacccccacccccctcctcgtcctcatcgccgacgacggtgcacccgcaccccatcccaactcctccgactcatcccccttctgcgccacgtacgaggaaatggtggacagcggcgatccggacttccagtgggccctgccggagaacgagtgggacccgatgacgctgaactacacttccggcacgacttcctcgcccaagggagtggtccactgccacagagggctcttcatcatcaccgtcaactctctcctcgactgggccgtacccagacagcccgtctatctctggacgcttccgatgttccacgccaacggctggagctacccgtacgcgatcgccgccgtcggtgggaccaacgtttgcgtccgcaaattcgacgcccatgtcatctacgacttgatcagacgacacggcgtcactcacatgtgcggtgcgccggtggtcctcaacatgctgaccaatgtaccgagcagcgagcctttgaggaacccggtccagatcatgaccgccggagctccgccgcccgcggcggttctgtctcggactgagtcgctgggattcgtg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

203

Amino Acids

22.04

Weight (kDa)

5.29

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 430
AccB7I CCANNNNNTGG 1 cut(s) 362
AccII CGCG 2 cut(s) 379, 574
AccIII TCCGGA 1 cut(s) 162
AclI AACGTT 1 cut(s) 404
AclWI GGATC 1 cut(s) 154
AcsI RAATTY 1 cut(s) 418
AcyI GRCGYC 3 cut(s) 20, 425, 461
AfaI GTAC 4 cut(s) 136, 311, 376, 512
AfiI CCNNNNNNNGG 3 cut(s) 303, 362, 395
AflIII ACRYGT 1 cut(s) 470
AhdI GACNNNNNGTC 2 cut(s) 322, 460
AloI GAACNNNNNNTCC 2 cut(s) 205, 237
AluBI AGCT 3 cut(s) 5, 368, 562
AluI AGCT 3 cut(s) 5, 368, 562
Alw21I GWGCWC 2 cut(s) 87, 564
Alw26I GTCTC 1 cut(s) 590
Alw44I GTGCAC 1 cut(s) 83
AlwI GGATC 1 cut(s) 154
Aor13HI TCCGGA 1 cut(s) 162
AoxI GGCC 2 cut(s) 176, 305
ApaI GGGCCC 1 cut(s) 180
ApaLI GTGCAC 1 cut(s) 83
ApeKI GCWGC 1 cut(s) 518
ApoI RAATTY 1 cut(s) 418
AsiSI GCGATCGC 1 cut(s) 383
AspLEI GCGC 2 cut(s) 130, 483
AspS9I GGNCC 8 cut(s) 176, 177, 198, 252, 305, 398, 489, 540
AsuC2I CCSGG 1 cut(s) 538
AsuHPI GGTGA 1 cut(s) 273
AvaII GGWCC 5 cut(s) 198, 252, 398, 489, 540
BaeGI GKGCMC 2 cut(s) 87, 180
BaeI ACNNNNGTAYC 2 cut(s) 494, 527
BanII GRGCYC 3 cut(s) 180, 272, 564
Bbv12I GWGCWC 2 cut(s) 87, 564
BbvI GCAGC 1 cut(s) 530
BccI CCATC 2 cut(s) 35, 104
BceAI ACGGC 4 cut(s) 292, 373, 375, 474
BcgI CGANNNNNNTGC 2 cut(s) 64, 98
BclI TGATCA 1 cut(s) 446
BcnI CCSGG 1 cut(s) 538
BcoDI GTCTC 1 cut(s) 590
BisI GCNGC 5 cut(s) 156, 386, 519, 568, 575
BlsI GCNGC 5 cut(s) 157, 387, 520, 569, 576
Bme1390I CCNGG 1 cut(s) 538
Bme18I GGWCC 5 cut(s) 198, 252, 398, 489, 540
BmeRI GACNNNNNGTC 2 cut(s) 322, 460
BmgT120I GGNCC 8 cut(s) 176, 177, 198, 252, 305, 398, 489, 540
BmiI GGNNCC 5 cut(s) 178, 199, 200, 399, 535
BmrFI CCNGG 1 cut(s) 538
BmrI ACTGGG 1 cut(s) 312
BmuI ACTGGG 1 cut(s) 312
BpmI CTGGAG 1 cut(s) 384
BpuMI CCSGG 1 cut(s) 538
BsaAI YACGTR 1 cut(s) 134
BsaHI GRCGYC 3 cut(s) 20, 425, 461
BsaJI CCNNGG 2 cut(s) 242, 572
BsaWI WCCGGW 1 cut(s) 162
BsaXI ACNNNNNCTCC 2 cut(s) 178, 208
Bsc4I CCNNNNNNNGG 3 cut(s) 303, 362, 395
Bse118I RCCGGY 1 cut(s) 483
Bse1I ACTGG 2 cut(s) 171, 307
BseAI TCCGGA 1 cut(s) 162
BseDI CCNNGG 2 cut(s) 242, 572
BseGI GGATG 2 cut(s) 96, 115
BseLI CCNNNNNNNGG 3 cut(s) 303, 362, 395
BseMII CTCAG 1 cut(s) 584
BseNI ACTGG 2 cut(s) 171, 307
BseRI GAGGAG 3 cut(s) 49, 96, 286
BseSI GKGCMC 2 cut(s) 87, 180
BseXI GCAGC 1 cut(s) 530
BseYI CCCAGC 1 cut(s) 600
Bsh1236I CGCG 2 cut(s) 379, 574
Bsh1285I CGRYCG 1 cut(s) 383
BshFI GGCC 2 cut(s) 178, 307
BsiEI CGRYCG 1 cut(s) 383
BsiHKAI GWGCWC 2 cut(s) 87, 564
BsiSI CCGG 6 cut(s) 163, 184, 224, 484, 538, 557
BsiWI CGTACG 2 cut(s) 134, 374
BslFI GGGAC 2 cut(s) 211, 411
BslI CCNNNNNNNGG 3 cut(s) 303, 362, 395
BsmAI GTCTC 1 cut(s) 590
BsmFI GGGAC 2 cut(s) 211, 411
BsnI GGCC 2 cut(s) 178, 307
Bsp120I GGGCCC 1 cut(s) 176
Bsp1286I GDGCHC 4 cut(s) 87, 180, 272, 564
Bsp13I TCCGGA 1 cut(s) 162
Bsp143I GATC 4 cut(s) 159, 380, 446, 545
BspANI GGCC 2 cut(s) 178, 307
BspCNI CTCAG 1 cut(s) 585
BspEI TCCGGA 1 cut(s) 162
BspFNI CGCG 2 cut(s) 379, 574
BspHI TCATGA 1 cut(s) 548
BspLI GGNNCC 5 cut(s) 178, 199, 200, 399, 535
BspPI GGATC 1 cut(s) 154
BspQI GCTCTTC 1 cut(s) 277
BsrFI RCCGGY 1 cut(s) 483
BsrI ACTGG 2 cut(s) 171, 307
BssAI RCCGGY 1 cut(s) 483
BssECI CCNNGG 2 cut(s) 242, 572
BssMI GATC 4 cut(s) 159, 380, 446, 545
BssNI GRCGYC 3 cut(s) 20, 425, 461
BssT1I CCWWGG 1 cut(s) 242
Bst4CI ACNGT 2 cut(s) 82, 285
Bst6I CTCTTC 2 cut(s) 37, 277
BstACI GRCGYC 3 cut(s) 20, 425, 461
BstBAI YACGTR 1 cut(s) 134
BstC8I GCNNGC 2 cut(s) 523, 572
BstDEI CTNAG 1 cut(s) 593
BstDSI CCRYGG 1 cut(s) 572
BstF5I GGATG 2 cut(s) 96, 115
BstFNI CGCG 2 cut(s) 379, 574
BstHHI GCGC 2 cut(s) 130, 483
BstKTI GATC 4 cut(s) 162, 383, 449, 548
BstMAI GTCTC 1 cut(s) 590
BstMBI GATC 4 cut(s) 159, 380, 446, 545
BstMCI CGRYCG 1 cut(s) 383
BstMWI GCNNNNNNNGC 1 cut(s) 385
BstNSI RCATGY 2 cut(s) 474, 501
BstSCI CCNGG 1 cut(s) 536
BstSLI GKGCMC 2 cut(s) 87, 180
BstUI CGCG 2 cut(s) 379, 574
BstV1I GCAGC 1 cut(s) 530
BstXI CCANNNNNNTGG 1 cut(s) 249
BsuRI GGCC 2 cut(s) 178, 307
BtgI CCRYGG 1 cut(s) 572
BtgZI GCGATG 1 cut(s) 54
BtsCI GGATG 2 cut(s) 96, 115
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 2 cut(s) 178, 255
Cac8I GCNNGC 2 cut(s) 523, 572
CciI TCATGA 1 cut(s) 548
CfoI GCGC 2 cut(s) 130, 483
Cfr10I RCCGGY 1 cut(s) 483
Cfr13I GGNCC 8 cut(s) 176, 177, 198, 252, 305, 398, 489, 540
Cfr42I CCGCGG 1 cut(s) 575
CseI GACGC 6 cut(s) 28, 216, 345, 399, 433, 450
Csp6I GTAC 4 cut(s) 135, 310, 375, 511
CviAII CATG 4 cut(s) 430, 471, 498, 549
CviJI RGCY 9 cut(s) 5, 178, 270, 307, 321, 362, 368, 525, 562
CviKI_1 RGCY 9 cut(s) 5, 178, 270, 307, 321, 362, 368, 525, 562
CviQI GTAC 4 cut(s) 135, 310, 375, 511
DdeI CTNAG 1 cut(s) 593
DpnI GATC 4 cut(s) 161, 382, 448, 547
DpnII GATC 4 cut(s) 159, 380, 446, 545
DrdI GACNNNNNNGTC 1 cut(s) 430
DriI GACNNNNNGTC 2 cut(s) 322, 460
DseDI GACNNNNNNGTC 1 cut(s) 430
Eam1104I CTCTTC 2 cut(s) 37, 277
Eam1105I GACNNNNNGTC 2 cut(s) 322, 460
EarI CTCTTC 2 cut(s) 37, 277
EciI GGCGGA 1 cut(s) 554
Ecl136II GAGCTC 1 cut(s) 562
Eco130I CCWWGG 1 cut(s) 242
Eco24I GRGCYC 3 cut(s) 180, 272, 564
Eco47I GGWCC 5 cut(s) 198, 252, 398, 489, 540
Eco53kI GAGCTC 1 cut(s) 562
EcoICRI GAGCTC 1 cut(s) 562
EcoO109I RGGNCCY 2 cut(s) 177, 198
EcoT14I CCWWGG 1 cut(s) 242
EcoT38I GRGCYC 3 cut(s) 180, 272, 564
ErhI CCWWGG 1 cut(s) 242
FaeI CATG 4 cut(s) 433, 474, 501, 552
FaiI YATR 4 cut(s) 431, 472, 499, 550
FaqI GGGAC 2 cut(s) 211, 411
FatI CATG 4 cut(s) 429, 470, 497, 548
FauI CCCGC 2 cut(s) 96, 579
FbaI TGATCA 1 cut(s) 446
Fnu4HI GCNGC 5 cut(s) 156, 386, 519, 568, 575
FokI GGATG 2 cut(s) 83, 102
FriOI GRGCYC 3 cut(s) 180, 272, 564
Fsp4HI GCNGC 5 cut(s) 156, 386, 519, 568, 575
GlaI GCGC 2 cut(s) 129, 482
GluI GCNGC 5 cut(s) 156, 386, 519, 568, 575
GsaI CCCAGC 1 cut(s) 604
GsuI CTGGAG 1 cut(s) 384
HaeIII GGCC 2 cut(s) 178, 307
HapII CCGG 6 cut(s) 163, 184, 224, 484, 538, 557
HgaI GACGC 6 cut(s) 28, 216, 345, 399, 433, 450
HhaI GCGC 2 cut(s) 130, 483
Hin1I GRCGYC 3 cut(s) 20, 425, 461
Hin1II CATG 4 cut(s) 433, 474, 501, 552
Hin6I GCGC 2 cut(s) 128, 481
HinP1I GCGC 2 cut(s) 128, 481
HincII GTYRAC 1 cut(s) 288
HindII GTYRAC 1 cut(s) 288
HinfI GANTC 3 cut(s) 112, 595, 605
HpaII CCGG 6 cut(s) 163, 184, 224, 484, 538, 557
HphI GGTGA 1 cut(s) 273
Hpy166II GTNNAC 4 cut(s) 85, 150, 255, 288
Hpy188I TCNGA 4 cut(s) 111, 344, 451, 590
Hpy188III TCNNGA 4 cut(s) 163, 333, 543, 549
Hpy8I GTNNAC 4 cut(s) 85, 150, 255, 288
Hpy99I CGWCG 5 cut(s) 22, 79, 82, 394, 427
HpyAV CCTTC 1 cut(s) 132
HpyCH4III ACNGT 2 cut(s) 82, 285
HpyCH4IV ACGT 2 cut(s) 133, 404
HpyCH4V TGCA 1 cut(s) 85
HpyF10VI GCNNNNNNNGC 1 cut(s) 385
HpyF3I CTNAG 1 cut(s) 593
HpySE526I ACGT 2 cut(s) 133, 404
Hsp92I GRCGYC 3 cut(s) 20, 425, 461
Hsp92II CATG 4 cut(s) 433, 474, 501, 552
HspAI GCGC 2 cut(s) 128, 481
KflI GGGWCCC 1 cut(s) 198
Kpn2I TCCGGA 1 cut(s) 162
Ksp22I TGATCA 1 cut(s) 446
KspI CCGCGG 1 cut(s) 575
Kzo9I GATC 4 cut(s) 159, 380, 446, 545
LguI GCTCTTC 1 cut(s) 277
LmnI GCTCC 4 cut(s) 10, 365, 559, 567
Lsp1109I GCAGC 1 cut(s) 530
MaeII ACGT 2 cut(s) 133, 404
MaeIII GTNAC 1 cut(s) 462
MalI GATC 4 cut(s) 161, 382, 448, 547
MboI GATC 4 cut(s) 159, 380, 446, 545
MboII GAAGA 2 cut(s) 24, 264
MhlI GDGCHC 4 cut(s) 87, 180, 272, 564
MluCI AATT 1 cut(s) 418
MlyI GAGTC 2 cut(s) 106, 604
MmeI TCCRAC 1 cut(s) 134
MroI TCCGGA 1 cut(s) 162
MslI CAYNNNNRTG 1 cut(s) 469
MspA1I CMGCKG 2 cut(s) 155, 574
MspI CCGG 6 cut(s) 163, 184, 224, 484, 538, 557
MspR9I CCNGG 1 cut(s) 538
MvnI CGCG 2 cut(s) 379, 574
MwoI GCNNNNNNNGC 1 cut(s) 385
NciI CCSGG 1 cut(s) 538
NdeII GATC 4 cut(s) 159, 380, 446, 545
NlaIII CATG 4 cut(s) 433, 474, 501, 552
NlaIV GGNNCC 5 cut(s) 178, 199, 200, 399, 535
NmuCI GTSAC 1 cut(s) 462
NspI RCATGY 2 cut(s) 474, 501
PagI TCATGA 1 cut(s) 548
PciI ACATGT 1 cut(s) 470
PciSI GCTCTTC 1 cut(s) 277
PcsI WCGNNNNNNNCGW 1 cut(s) 305
PfeI GAWTC 1 cut(s) 605
Pfl23II CGTACG 2 cut(s) 134, 374
PflFI GACNNNGTC 1 cut(s) 594
PflMI CCANNNNNTGG 1 cut(s) 362
PkrI GCNGC 5 cut(s) 157, 387, 520, 569, 576
Ple19I CGATCG 1 cut(s) 383
PleI GAGTC 2 cut(s) 106, 603
PpsI GAGTC 2 cut(s) 106, 603
Ppu21I YACGTR 1 cut(s) 134
PpuMI RGGWCCY 1 cut(s) 198
PscI ACATGT 1 cut(s) 470
Psp124BI GAGCTC 1 cut(s) 564
Psp1406I AACGTT 1 cut(s) 404
Psp5II RGGWCCY 1 cut(s) 198
PspFI CCCAGC 1 cut(s) 600
PspLI CGTACG 2 cut(s) 134, 374
PspN4I GGNNCC 5 cut(s) 178, 199, 200, 399, 535
PspOMI GGGCCC 1 cut(s) 176
PspPI GGNCC 8 cut(s) 176, 177, 198, 252, 305, 398, 489, 540
PspPPI RGGWCCY 1 cut(s) 198
PsyI GACNNNGTC 1 cut(s) 594
PvuI CGATCG 1 cut(s) 383
RgaI GCGATCGC 1 cut(s) 383
RsaI GTAC 4 cut(s) 136, 311, 376, 512
RsaNI GTAC 4 cut(s) 135, 310, 375, 511
RseI CAYNNNNRTG 1 cut(s) 469
SacI GAGCTC 1 cut(s) 564
SacII CCGCGG 1 cut(s) 575
SapI GCTCTTC 1 cut(s) 277
SatI GCNGC 5 cut(s) 156, 386, 519, 568, 575
Sau3AI GATC 4 cut(s) 159, 380, 446, 545
Sau96I GGNCC 8 cut(s) 176, 177, 198, 252, 305, 398, 489, 540
SchI GAGTC 2 cut(s) 106, 604
ScrFI CCNGG 1 cut(s) 538
SduI GDGCHC 4 cut(s) 87, 180, 272, 564
SetI ASST 5 cut(s) 7, 136, 370, 407, 564
SfaAI GCGATCGC 1 cut(s) 383
Sfr303I CCGCGG 1 cut(s) 575
SgfI GCGATCGC 1 cut(s) 383
SgrAI CRCCGGYG 1 cut(s) 483
SgrBI CCGCGG 1 cut(s) 575
SinI GGWCC 5 cut(s) 198, 252, 398, 489, 540
SmiMI CAYNNNNRTG 1 cut(s) 469
Sse9I AATT 1 cut(s) 418
SstI GAGCTC 1 cut(s) 564
StyD4I CCNGG 1 cut(s) 536
StyI CCWWGG 1 cut(s) 242
TaaI ACNGT 2 cut(s) 82, 285
TaiI ACGT 2 cut(s) 136, 407
TaqI TCGA 3 cut(s) 17, 299, 422
TasI AATT 1 cut(s) 418
TauI GCSGC 4 cut(s) 158, 388, 570, 577
TfiI GAWTC 1 cut(s) 605
TscAI CASTG 2 cut(s) 178, 262
TseFI GTSAC 1 cut(s) 462
TseI GCWGC 1 cut(s) 518
Tsp45I GTSAC 1 cut(s) 462
TspDTI ATGAA 1 cut(s) 264
TspRI CASTG 2 cut(s) 178, 262
Tth111I GACNNNGTC 1 cut(s) 594
Van91I CCANNNNNTGG 1 cut(s) 362
VneI GTGCAC 1 cut(s) 83
VpaK11BI GGWCC 5 cut(s) 198, 252, 398, 489, 540
XapI RAATTY 1 cut(s) 418
XceI RCATGY 2 cut(s) 474, 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.