Rh4CG321700

Acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0016444
Physical Location & Seq
Forward (+)
58395756 .. 58395968
213 bp
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UTR
Exon/CDS
Intron
Rh4CG321700.1

Sequence Viewer

Length: 213 bp
ATGGTGGAGAGCGGCGACCCGGACTTCCAGTGGGCCCTGCCGGAGAACGAGTGGGACCTGATGACGCTGAACTACACTTCCGGCACGACTTCCTCGCCCAAGGGAGTGGTCCACTGCCACAGAGGGCTCTTCATCATCACCGTCAACTCTCTCCTCGACTGGGCCGTACCCAGACAGCCCGTCTATCTCTGGACGCTTCCGATGTTCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

70

Amino Acids

8.05

Weight (kDa)

4.93

Isoelectric Point (pI)

29.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 14 - 70 3.3e-14 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 12
AciI CCGC 1 cut(s) 12
AfaI GTAC 1 cut(s) 168
AfiI CCNNNNNNNGG 1 cut(s) 160
AhdI GACNNNNNGTC 1 cut(s) 179
AloI GAACNNNNNNTCC 2 cut(s) 62, 94
AoxI GGCC 2 cut(s) 33, 162
ApaI GGGCCC 1 cut(s) 37
AspS9I GGNCC 5 cut(s) 33, 34, 55, 109, 162
AsuC2I CCSGG 1 cut(s) 20
AsuHPI GGTGA 1 cut(s) 130
AvaII GGWCC 2 cut(s) 55, 109
BaeGI GKGCMC 1 cut(s) 37
BanII GRGCYC 2 cut(s) 37, 129
BceAI ACGGC 1 cut(s) 149
BcnI CCSGG 1 cut(s) 20
BisI GCNGC 1 cut(s) 13
BlsI GCNGC 1 cut(s) 14
Bme1390I CCNGG 1 cut(s) 20
Bme18I GGWCC 2 cut(s) 55, 109
BmeRI GACNNNNNGTC 1 cut(s) 179
BmgT120I GGNCC 5 cut(s) 33, 34, 55, 109, 162
BmiI GGNNCC 2 cut(s) 35, 56
BmrFI CCNGG 1 cut(s) 20
BmrI ACTGGG 1 cut(s) 169
BmuI ACTGGG 1 cut(s) 169
BpuMI CCSGG 1 cut(s) 20
BsaJI CCNNGG 1 cut(s) 99
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 1 cut(s) 160
Bse1I ACTGG 2 cut(s) 28, 164
BseDI CCNNGG 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 160
BseNI ACTGG 2 cut(s) 28, 164
BseRI GAGGAG 1 cut(s) 143
BseSI GKGCMC 1 cut(s) 37
BshFI GGCC 2 cut(s) 35, 164
BsiSI CCGG 3 cut(s) 20, 41, 81
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 1 cut(s) 160
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 2 cut(s) 35, 164
Bsp120I GGGCCC 1 cut(s) 33
Bsp1286I GDGCHC 2 cut(s) 37, 129
BspACI CCGC 1 cut(s) 12
BspANI GGCC 2 cut(s) 35, 164
BspLI GGNNCC 2 cut(s) 35, 56
BspQI GCTCTTC 1 cut(s) 134
BsrBI CCGCTC 1 cut(s) 12
BsrI ACTGG 2 cut(s) 28, 164
BssECI CCNNGG 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 142
Bst6I CTCTTC 1 cut(s) 134
BstSCI CCNGG 1 cut(s) 18
BstSLI GKGCMC 1 cut(s) 37
BstXI CCANNNNNNTGG 1 cut(s) 106
BsuRI GGCC 2 cut(s) 35, 164
BtsI GCAGTG 1 cut(s) 112
BtsIMutI CAGTG 3 cut(s) 35, 112, 208
Cfr13I GGNCC 5 cut(s) 33, 34, 55, 109, 162
CseI GACGC 2 cut(s) 73, 202
Csp6I GTAC 1 cut(s) 167
CviJI RGCY 4 cut(s) 35, 127, 164, 178
CviKI_1 RGCY 4 cut(s) 35, 127, 164, 178
CviQI GTAC 1 cut(s) 167
DriI GACNNNNNGTC 1 cut(s) 179
Eam1104I CTCTTC 1 cut(s) 134
Eam1105I GACNNNNNGTC 1 cut(s) 179
EarI CTCTTC 1 cut(s) 134
Eco130I CCWWGG 1 cut(s) 99
Eco24I GRGCYC 2 cut(s) 37, 129
Eco47I GGWCC 2 cut(s) 55, 109
EcoO109I RGGNCCY 2 cut(s) 34, 55
EcoT14I CCWWGG 1 cut(s) 99
EcoT38I GRGCYC 2 cut(s) 37, 129
ErhI CCWWGG 1 cut(s) 99
FaqI GGGAC 1 cut(s) 68
Fnu4HI GCNGC 1 cut(s) 13
FriOI GRGCYC 2 cut(s) 37, 129
Fsp4HI GCNGC 1 cut(s) 13
GluI GCNGC 1 cut(s) 13
HaeIII GGCC 2 cut(s) 35, 164
HapII CCGG 3 cut(s) 20, 41, 81
HgaI GACGC 2 cut(s) 73, 202
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HpaII CCGG 3 cut(s) 20, 41, 81
HphI GGTGA 1 cut(s) 130
Hpy166II GTNNAC 2 cut(s) 112, 145
Hpy188I TCNGA 1 cut(s) 201
Hpy188III TCNNGA 1 cut(s) 190
Hpy8I GTNNAC 2 cut(s) 112, 145
HpyCH4III ACNGT 1 cut(s) 142
LguI GCTCTTC 1 cut(s) 134
LpnPI CCDG 9 cut(s) 33, 41, 50, 54, 71, 94, 145, 175, 184
MbiI CCGCTC 1 cut(s) 12
MboII GAAGA 1 cut(s) 121
MhlI GDGCHC 2 cut(s) 37, 129
MnlI CCTC 3 cut(s) 103, 116, 164
MspI CCGG 3 cut(s) 20, 41, 81
MspR9I CCNGG 1 cut(s) 20
NciI CCSGG 1 cut(s) 20
NlaIV GGNNCC 2 cut(s) 35, 56
PciSI GCTCTTC 1 cut(s) 134
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PkrI GCNGC 1 cut(s) 14
PpuMI RGGWCCY 1 cut(s) 55
Psp5II RGGWCCY 1 cut(s) 55
PspN4I GGNNCC 2 cut(s) 35, 56
PspOMI GGGCCC 1 cut(s) 33
PspPI GGNCC 5 cut(s) 33, 34, 55, 109, 162
PspPPI RGGWCCY 1 cut(s) 55
RsaI GTAC 1 cut(s) 168
RsaNI GTAC 1 cut(s) 167
SapI GCTCTTC 1 cut(s) 134
SatI GCNGC 1 cut(s) 13
Sau96I GGNCC 5 cut(s) 33, 34, 55, 109, 162
ScrFI CCNGG 1 cut(s) 20
SduI GDGCHC 2 cut(s) 37, 129
SetI ASST 1 cut(s) 60
SinI GGWCC 2 cut(s) 55, 109
SsiI CCGC 1 cut(s) 12
StyD4I CCNGG 1 cut(s) 18
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 1 cut(s) 142
TaqI TCGA 1 cut(s) 156
TauI GCSGC 1 cut(s) 15
TscAI CASTG 2 cut(s) 35, 119
TspDTI ATGAA 1 cut(s) 121
TspRI CASTG 2 cut(s) 35, 119
VpaK11BI GGWCC 2 cut(s) 55, 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.