Rmu_sc0028450.1_g000001

Alkaline and neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0028450.1
OG0025463
Physical Location & Seq
Forward (+)
1 .. 418
418 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0028450.1_g000001.1.cds

Sequence Viewer

Length: 418 bp
attggccaagaaagatccattgtcgccggagaagtgtacgggaacgtccgcgacgccgccgtctacagggaaggcaaagaagtcggtgaaactgaaactgcagaggcccgacgaagctccgaagcagacgacgtcgctgctgatgtcgtttcaaagtatgaggagtcctgagttgagtgcactcatcgtggaatcttcgaaggaatcttcggcggctatggtggaggaggcctgggagagactcaagaagtcgtacgtttactacagaggcaagccggtggggactcttgctgctatggatccaacggccgaggctctaaactacaatcaggtcgttcctgcagctcttcttgatctttatcaaacgatacttgtttttcttgccttattttggtccctaatttggcaattcctgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

138

Amino Acids

15.28

Weight (kDa)

9.19

Isoelectric Point (pI)

41.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025463)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0028450.1_g000001 Rmu_sc0028451.1_g000001 Rmu_ssc0000368.1_g000048

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 59
AatII GACGTC 1 cut(s) 135
AccI GTMKAC 1 cut(s) 63
AccII CGCG 1 cut(s) 51
AciI CCGC 3 cut(s) 49, 57, 213
AclWI GGATC 3 cut(s) 9, 294, 307
AcoI YGGCCR 2 cut(s) 4, 307
AcyI GRCGYC 2 cut(s) 54, 132
AfaI GTAC 2 cut(s) 38, 255
AfiI CCNNNNNNNGG 3 cut(s) 66, 391, 403
AgsI TTSAA 1 cut(s) 153
AjnI CCWGG 1 cut(s) 231
AluBI AGCT 2 cut(s) 117, 345
AluI AGCT 2 cut(s) 117, 345
Alw21I GWGCWC 1 cut(s) 182
Alw26I GTCTC 1 cut(s) 233
Alw44I GTGCAC 1 cut(s) 178
AlwI GGATC 3 cut(s) 9, 294, 307
AoxI GGCC 4 cut(s) 4, 105, 229, 307
ApaLI GTGCAC 1 cut(s) 178
ApeKI GCWGC 3 cut(s) 137, 291, 342
AspS9I GGNCC 2 cut(s) 106, 394
AsuHPI GGTGA 1 cut(s) 98
AsuII TTCGAA 1 cut(s) 198
AvaII GGWCC 1 cut(s) 394
BaeGI GKGCMC 1 cut(s) 182
BalI TGGCCA 1 cut(s) 6
BamHI GGATCC 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 182
BbvI GCAGC 3 cut(s) 124, 278, 354
BceAI ACGGC 2 cut(s) 44, 322
BcgI CGANNNNNNTGC 4 cut(s) 64, 98, 119, 153
BciT130I CCWGG 1 cut(s) 233
BcoDI GTCTC 1 cut(s) 233
BfmI CTRYAG 4 cut(s) 64, 99, 263, 340
BisI GCNGC 5 cut(s) 57, 138, 214, 292, 343
BlsI GCNGC 5 cut(s) 58, 139, 215, 293, 344
Bme1390I CCNGG 1 cut(s) 233
Bme18I GGWCC 1 cut(s) 394
BmgT120I GGNCC 2 cut(s) 106, 394
BmiI GGNNCC 2 cut(s) 301, 396
BmrFI CCNGG 1 cut(s) 233
Bpu14I TTCGAA 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 228
BsaBI GATNNNNATC 1 cut(s) 358
BsaHI GRCGYC 2 cut(s) 54, 132
BsaJI CCNNGG 2 cut(s) 232, 310
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 391, 403
Bse118I RCCGGY 1 cut(s) 275
Bse8I GATNNNNATC 1 cut(s) 358
BseBI CCWGG 1 cut(s) 233
BseDI CCNNGG 2 cut(s) 232, 310
BseJI GATNNNNATC 1 cut(s) 358
BseLI CCNNNNNNNGG 3 cut(s) 66, 391, 403
BseMII CTCAG 1 cut(s) 160
BseRI GAGGAG 2 cut(s) 176, 240
BseSI GKGCMC 1 cut(s) 182
BseX3I CGGCCG 1 cut(s) 307
BseXI GCAGC 3 cut(s) 124, 278, 354
Bsh1236I CGCG 1 cut(s) 51
Bsh1285I CGRYCG 1 cut(s) 310
BshFI GGCC 4 cut(s) 6, 107, 231, 309
BsiEI CGRYCG 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 182
BsiSI CCGG 2 cut(s) 27, 276
BsiWI CGTACG 1 cut(s) 253
BslFI GGGAC 2 cut(s) 296, 380
BslI CCNNNNNNNGG 3 cut(s) 66, 391, 403
BsmAI GTCTC 1 cut(s) 233
BsmFI GGGAC 2 cut(s) 296, 380
BsnI GGCC 4 cut(s) 6, 107, 231, 309
Bsp119I TTCGAA 1 cut(s) 198
Bsp1286I GDGCHC 1 cut(s) 182
Bsp143I GATC 3 cut(s) 14, 299, 353
BspACI CCGC 3 cut(s) 49, 57, 213
BspANI GGCC 4 cut(s) 6, 107, 231, 309
BspCNI CTCAG 1 cut(s) 161
BspFNI CGCG 1 cut(s) 51
BspLI GGNNCC 2 cut(s) 301, 396
BspMAI CTGCAG 2 cut(s) 103, 344
BspPI GGATC 3 cut(s) 9, 294, 307
BspQI GCTCTTC 1 cut(s) 352
BspT104I TTCGAA 1 cut(s) 198
BsrFI RCCGGY 1 cut(s) 275
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 2 cut(s) 232, 310
BssMI GATC 3 cut(s) 14, 299, 353
BssNI GRCGYC 2 cut(s) 54, 132
Bst2UI CCWGG 1 cut(s) 233
Bst6I CTCTTC 1 cut(s) 352
BstACI GRCGYC 2 cut(s) 54, 132
BstBI TTCGAA 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 273
BstDEI CTNAG 1 cut(s) 169
BstFNI CGCG 1 cut(s) 51
BstKTI GATC 3 cut(s) 17, 302, 356
BstMAI GTCTC 1 cut(s) 233
BstMBI GATC 3 cut(s) 14, 299, 353
BstMCI CGRYCG 1 cut(s) 310
BstNI CCWGG 1 cut(s) 233
BstSCI CCNGG 1 cut(s) 231
BstSFI CTRYAG 4 cut(s) 64, 99, 263, 340
BstSLI GKGCMC 1 cut(s) 182
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 3 cut(s) 124, 278, 354
BstX2I RGATCY 2 cut(s) 14, 299
BstYI RGATCY 2 cut(s) 14, 299
BstZI CGGCCG 1 cut(s) 307
BsuRI GGCC 4 cut(s) 6, 107, 231, 309
Cac8I GCNNGC 1 cut(s) 273
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 2 cut(s) 106, 394
CseI GACGC 1 cut(s) 62
Csp6I GTAC 2 cut(s) 37, 254
CviJI RGCY 9 cut(s) 6, 107, 117, 216, 231, 275, 309, 315, 345
CviKI_1 RGCY 9 cut(s) 6, 107, 117, 216, 231, 275, 309, 315, 345
CviQI GTAC 2 cut(s) 37, 254
DdeI CTNAG 1 cut(s) 169
DpnI GATC 3 cut(s) 16, 301, 355
DpnII GATC 3 cut(s) 14, 299, 353
DrdI GACNNNNNNGTC 1 cut(s) 59
DseDI GACNNNNNNGTC 1 cut(s) 59
EaeI YGGCCR 2 cut(s) 4, 307
EagI CGGCCG 1 cut(s) 307
Eam1104I CTCTTC 1 cut(s) 352
EarI CTCTTC 1 cut(s) 352
EclXI CGGCCG 1 cut(s) 307
Eco147I AGGCCT 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 394
Eco52I CGGCCG 1 cut(s) 307
EcoRII CCWGG 1 cut(s) 231
FaiI YATR 3 cut(s) 159, 219, 297
FaqI GGGAC 2 cut(s) 296, 380
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 5 cut(s) 57, 138, 214, 292, 343
Fsp4HI GCNGC 5 cut(s) 57, 138, 214, 292, 343
GluI GCNGC 5 cut(s) 57, 138, 214, 292, 343
HaeIII GGCC 4 cut(s) 6, 107, 231, 309
HapII CCGG 2 cut(s) 27, 276
HgaI GACGC 1 cut(s) 62
Hin1I GRCGYC 2 cut(s) 54, 132
HinfI GANTC 5 cut(s) 164, 192, 204, 241, 284
HpaII CCGG 2 cut(s) 27, 276
HphI GGTGA 1 cut(s) 98
Hpy166II GTNNAC 4 cut(s) 37, 64, 180, 260
Hpy188I TCNGA 1 cut(s) 121
Hpy188III TCNNGA 3 cut(s) 168, 245, 351
Hpy8I GTNNAC 4 cut(s) 37, 64, 180, 260
Hpy99I CGWCG 4 cut(s) 56, 114, 134, 137
HpyAV CCTTC 2 cut(s) 65, 194
HpyCH4IV ACGT 3 cut(s) 45, 132, 256
HpyCH4V TGCA 3 cut(s) 101, 180, 342
HpyF3I CTNAG 1 cut(s) 169
HpySE526I ACGT 3 cut(s) 45, 132, 256
Hsp92I GRCGYC 2 cut(s) 54, 132
Kzo9I GATC 3 cut(s) 14, 299, 353
LguI GCTCTTC 1 cut(s) 352
LmnI GCTCC 1 cut(s) 122
LpnPI CCDG 8 cut(s) 40, 52, 181, 218, 245, 289, 315, 352
Lsp1109I GCAGC 3 cut(s) 124, 278, 354
MaeII ACGT 3 cut(s) 45, 132, 256
MalI GATC 3 cut(s) 16, 301, 355
MboI GATC 3 cut(s) 14, 299, 353
MboII GAAGA 3 cut(s) 187, 199, 339
MflI RGATCY 2 cut(s) 14, 299
MhlI GDGCHC 1 cut(s) 182
MlsI TGGCCA 1 cut(s) 6
MluCI AATT 2 cut(s) 400, 408
MluNI TGGCCA 1 cut(s) 6
MlyI GAGTC 3 cut(s) 173, 235, 278
MmeI TCCRAC 1 cut(s) 327
MnlI CCTC 6 cut(s) 97, 154, 218, 221, 261, 305
Mox20I TGGCCA 1 cut(s) 6
MscI TGGCCA 1 cut(s) 6
Msp20I TGGCCA 1 cut(s) 6
MspI CCGG 2 cut(s) 27, 276
MspR9I CCNGG 1 cut(s) 233
MvaI CCWGG 1 cut(s) 233
MvnI CGCG 1 cut(s) 51
NdeII GATC 3 cut(s) 14, 299, 353
NlaIV GGNNCC 2 cut(s) 301, 396
NmeAIII GCCGAG 1 cut(s) 335
NspV TTCGAA 1 cut(s) 198
PceI AGGCCT 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 352
PfeI GAWTC 2 cut(s) 192, 204
Pfl23II CGTACG 1 cut(s) 253
PflFI GACNNNGTC 1 cut(s) 131
PkrI GCNGC 5 cut(s) 58, 139, 215, 293, 344
PleI GAGTC 3 cut(s) 172, 235, 278
PpsI GAGTC 3 cut(s) 172, 235, 278
Psp6I CCWGG 1 cut(s) 231
PspGI CCWGG 1 cut(s) 231
PspLI CGTACG 1 cut(s) 253
PspN4I GGNNCC 2 cut(s) 301, 396
PspPI GGNCC 2 cut(s) 106, 394
PstI CTGCAG 2 cut(s) 103, 344
PsuI RGATCY 2 cut(s) 14, 299
PsyI GACNNNGTC 1 cut(s) 131
RsaI GTAC 2 cut(s) 38, 255
RsaNI GTAC 2 cut(s) 37, 254
SapI GCTCTTC 1 cut(s) 352
SatI GCNGC 5 cut(s) 57, 138, 214, 292, 343
Sau3AI GATC 3 cut(s) 14, 299, 353
Sau96I GGNCC 2 cut(s) 106, 394
SchI GAGTC 3 cut(s) 173, 235, 278
ScrFI CCNGG 1 cut(s) 233
SduI GDGCHC 1 cut(s) 182
SetI ASST 6 cut(s) 48, 119, 135, 259, 334, 347
SfcI CTRYAG 4 cut(s) 64, 99, 263, 340
SfuI TTCGAA 1 cut(s) 198
SinI GGWCC 1 cut(s) 394
SmlI CTYRAG 1 cut(s) 243
SmoI CTYRAG 1 cut(s) 243
Sse9I AATT 2 cut(s) 400, 408
SseBI AGGCCT 1 cut(s) 231
SsiI CCGC 3 cut(s) 49, 57, 213
StuI AGGCCT 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 231
TaiI ACGT 3 cut(s) 48, 135, 259
TaqI TCGA 1 cut(s) 198
TasI AATT 2 cut(s) 400, 408
TauI GCSGC 2 cut(s) 59, 216
TfiI GAWTC 2 cut(s) 192, 204
TseI GCWGC 3 cut(s) 137, 291, 342
Tth111I GACNNNGTC 1 cut(s) 131
VneI GTGCAC 1 cut(s) 178
VpaK11BI GGWCC 1 cut(s) 394
XmiI GTMKAC 1 cut(s) 63
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.