Rmu_ssc0000368.1_g000048

Alkaline and neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000368.1
OG0025463
Physical Location & Seq
Forward (+)
247454 .. 248206
753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000368.1_g000048.1.cds

Sequence Viewer

Length: 753 bp
atgtcgaaaccgctcggagaatacctcaaggaagaaaaggaagcagaggaggcggccgctcggcctccgccttctgctcatgagacgttgccggagatagactcgccggggtcggatccatcgatgaagctttcggatttcactgctgatgacgactcggtgtctttcgggcttcagcggctcaaggagattgctgaagcagccgagaagaagaagaaggcggagaagaataagaagaagaaggagaagaagaagatgaagaagctgcaggcggagaaagatgtcggctcttccaagactgaaggtgcgagtgcgactccgagtgatgaggatgactcgacgaaatcggaagaaggttctggaacctgtgagaagagcgagagtgccgaattggccaagaaagatccattgtcgccggagaagtgtacgggaacgtccgcgacgccgccgtctacagggaaggcaaagaaatcggtgaaactgaaactgcagaggcccgacgaagctccgaagcagacaacgtcgctgctgatgtcgtttcaaagtatgaggagtcctgagttgagtgcactcatcgtggaatcttcgaaggaatcttcggcggctatggtggaggaggcctgggagagactcaagaagtcgtacgtttactacagaggcaagccggtggggactcttgctgctatggatccaacggccgaggctctaaactacaatcaggtcgttcctgcagctcttcctcttgatctttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

250

Amino Acids

27.06

Weight (kDa)

5.82

Isoelectric Point (pI)

50.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025463)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0028450.1_g000001 Rmu_sc0028451.1_g000001 Rmu_ssc0000368.1_g000048

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 448
AccBSI CCGCTC 2 cut(s) 13, 59
AccI GTMKAC 1 cut(s) 452
AccII CGCG 1 cut(s) 440
AclWI GGATC 5 cut(s) 110, 123, 398, 683, 696
AcoI YGGCCR 3 cut(s) 54, 393, 696
AcuI CTGAAG 3 cut(s) 158, 216, 321
AcyI GRCGYC 1 cut(s) 443
AfaI GTAC 2 cut(s) 427, 644
AfiI CCNNNNNNNGG 1 cut(s) 455
AgsI TTSAA 1 cut(s) 542
AhdI GACNNNNNGTC 1 cut(s) 160
AjnI CCWGG 1 cut(s) 620
AluBI AGCT 4 cut(s) 130, 265, 506, 734
AluI AGCT 4 cut(s) 130, 265, 506, 734
Alw21I GWGCWC 1 cut(s) 571
Alw26I GTCTC 2 cut(s) 77, 622
Alw44I GTGCAC 1 cut(s) 567
AlwI GGATC 5 cut(s) 110, 123, 398, 683, 696
AoxI GGCC 6 cut(s) 54, 62, 393, 494, 618, 696
ApaLI GTGCAC 1 cut(s) 567
ApeKI GCWGC 5 cut(s) 200, 265, 526, 680, 731
AspS9I GGNCC 1 cut(s) 495
AsuC2I CCSGG 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 487
AsuII TTCGAA 1 cut(s) 587
BaeGI GKGCMC 1 cut(s) 571
BalI TGGCCA 1 cut(s) 395
BamHI GGATCC 2 cut(s) 115, 688
Bbv12I GWGCWC 1 cut(s) 571
BbvI GCAGC 5 cut(s) 212, 252, 513, 667, 743
BccI CCATC 1 cut(s) 127
BceAI ACGGC 2 cut(s) 433, 711
BcgI CGANNNNNNTGC 2 cut(s) 453, 487
BciT130I CCWGG 1 cut(s) 622
BcnI CCSGG 1 cut(s) 108
BcoDI GTCTC 2 cut(s) 77, 622
BfmI CTRYAG 5 cut(s) 266, 453, 488, 652, 729
BglI GCCNNNNNGGC 1 cut(s) 392
Bme1390I CCNGG 2 cut(s) 108, 622
BmeRI GACNNNNNGTC 1 cut(s) 160
BmgT120I GGNCC 1 cut(s) 495
BmiI GGNNCC 3 cut(s) 117, 364, 690
BmrFI CCNGG 2 cut(s) 108, 622
BplI GAGNNNNNCTC 8 cut(s) 9, 41, 86, 118, 301, 333, 320, 352
Bpu14I TTCGAA 1 cut(s) 587
BpuEI CTTGAG 3 cut(s) 11, 167, 617
BpuMI CCSGG 1 cut(s) 108
Bsa29I ATCGAT 1 cut(s) 122
BsaHI GRCGYC 1 cut(s) 443
BsaJI CCNNGG 3 cut(s) 107, 621, 699
Bsc4I CCNNNNNNNGG 1 cut(s) 455
Bse118I RCCGGY 1 cut(s) 664
BseBI CCWGG 1 cut(s) 622
BseCI ATCGAT 1 cut(s) 122
BseDI CCNNGG 3 cut(s) 107, 621, 699
BseGI GGATG 1 cut(s) 337
BseLI CCNNNNNNNGG 1 cut(s) 455
BseMII CTCAG 1 cut(s) 549
BseRI GAGGAG 3 cut(s) 62, 565, 629
BseSI GKGCMC 1 cut(s) 571
BseX3I CGGCCG 2 cut(s) 54, 696
BseXI GCAGC 5 cut(s) 212, 252, 513, 667, 743
Bsh1236I CGCG 1 cut(s) 440
Bsh1285I CGRYCG 2 cut(s) 57, 699
BshFI GGCC 6 cut(s) 56, 64, 395, 496, 620, 698
BshVI ATCGAT 1 cut(s) 122
BsiEI CGRYCG 2 cut(s) 57, 699
BsiHKAI GWGCWC 1 cut(s) 571
BsiSI CCGG 4 cut(s) 92, 107, 416, 665
BsiWI CGTACG 1 cut(s) 642
BslFI GGGAC 1 cut(s) 685
BslI CCNNNNNNNGG 1 cut(s) 455
BsmAI GTCTC 2 cut(s) 77, 622
BsmBI CGTCTC 1 cut(s) 77
BsmFI GGGAC 1 cut(s) 685
BsnI GGCC 6 cut(s) 56, 64, 395, 496, 620, 698
Bsp119I TTCGAA 1 cut(s) 587
Bsp1286I GDGCHC 1 cut(s) 571
Bsp143I GATC 4 cut(s) 115, 403, 688, 745
BspANI GGCC 6 cut(s) 56, 64, 395, 496, 620, 698
BspCNI CTCAG 1 cut(s) 550
BspDI ATCGAT 1 cut(s) 122
BspFNI CGCG 1 cut(s) 440
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 3 cut(s) 117, 364, 690
BspMAI CTGCAG 3 cut(s) 270, 492, 733
BspPI GGATC 5 cut(s) 110, 123, 398, 683, 696
BspQI GCTCTTC 3 cut(s) 295, 368, 741
BspT104I TTCGAA 1 cut(s) 587
BsrBI CCGCTC 2 cut(s) 13, 59
BsrFI RCCGGY 1 cut(s) 664
BssAI RCCGGY 1 cut(s) 664
BssECI CCNNGG 3 cut(s) 107, 621, 699
BssMI GATC 4 cut(s) 115, 403, 688, 745
BssNI GRCGYC 1 cut(s) 443
Bst2UI CCWGG 1 cut(s) 622
Bst6I CTCTTC 3 cut(s) 295, 368, 741
BstACI GRCGYC 1 cut(s) 443
BstBI TTCGAA 1 cut(s) 587
BstC8I GCNNGC 2 cut(s) 270, 662
BstDEI CTNAG 1 cut(s) 558
BstF5I GGATG 1 cut(s) 337
BstFNI CGCG 1 cut(s) 440
BstKTI GATC 4 cut(s) 118, 406, 691, 748
BstMAI GTCTC 2 cut(s) 77, 622
BstMBI GATC 4 cut(s) 115, 403, 688, 745
BstMCI CGRYCG 2 cut(s) 57, 699
BstMWI GCNNNNNNNGC 4 cut(s) 50, 178, 200, 392
BstNI CCWGG 1 cut(s) 622
BstSCI CCNGG 2 cut(s) 106, 620
BstSFI CTRYAG 5 cut(s) 266, 453, 488, 652, 729
BstSLI GKGCMC 1 cut(s) 571
BstUI CGCG 1 cut(s) 440
BstV1I GCAGC 5 cut(s) 212, 252, 513, 667, 743
BstX2I RGATCY 3 cut(s) 115, 403, 688
BstYI RGATCY 3 cut(s) 115, 403, 688
BstZI CGGCCG 2 cut(s) 54, 696
Bsu15I ATCGAT 1 cut(s) 122
BsuRI GGCC 6 cut(s) 56, 64, 395, 496, 620, 698
BsuTUI ATCGAT 1 cut(s) 122
BtsCI GGATG 1 cut(s) 337
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 1 cut(s) 141
Cac8I GCNNGC 2 cut(s) 270, 662
CciI TCATGA 1 cut(s) 79
CciNI GCGGCCGC 1 cut(s) 54
Cfr10I RCCGGY 1 cut(s) 664
Cfr13I GGNCC 1 cut(s) 495
ClaI ATCGAT 1 cut(s) 122
CseI GACGC 1 cut(s) 451
Csp6I GTAC 2 cut(s) 426, 643
CviAII CATG 1 cut(s) 80
CviQI GTAC 2 cut(s) 426, 643
DdeI CTNAG 1 cut(s) 558
DpnI GATC 4 cut(s) 117, 405, 690, 747
DpnII GATC 4 cut(s) 115, 403, 688, 745
DrdI GACNNNNNNGTC 1 cut(s) 448
DriI GACNNNNNGTC 1 cut(s) 160
DseDI GACNNNNNNGTC 1 cut(s) 448
EaeI YGGCCR 3 cut(s) 54, 393, 696
EagI CGGCCG 2 cut(s) 54, 696
Eam1104I CTCTTC 3 cut(s) 295, 368, 741
Eam1105I GACNNNNNGTC 1 cut(s) 160
EarI CTCTTC 3 cut(s) 295, 368, 741
EciI GGCGGA 3 cut(s) 57, 236, 287
EclXI CGGCCG 2 cut(s) 54, 696
Eco147I AGGCCT 1 cut(s) 620
Eco52I CGGCCG 2 cut(s) 54, 696
Eco57I CTGAAG 3 cut(s) 158, 216, 321
EcoRII CCWGG 1 cut(s) 620
Esp3I CGTCTC 1 cut(s) 77
FaeI CATG 1 cut(s) 83
FaiI YATR 4 cut(s) 81, 548, 608, 686
FaqI GGGAC 1 cut(s) 685
FatI CATG 1 cut(s) 79
FblI GTMKAC 1 cut(s) 452
FokI GGATG 1 cut(s) 344
HaeIII GGCC 6 cut(s) 56, 64, 395, 496, 620, 698
HapII CCGG 4 cut(s) 92, 107, 416, 665
HgaI GACGC 1 cut(s) 451
Hin1I GRCGYC 1 cut(s) 443
Hin1II CATG 1 cut(s) 83
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 9 cut(s) 101, 155, 316, 335, 553, 581, 593, 630, 673
HpaII CCGG 4 cut(s) 92, 107, 416, 665
HphI GGTGA 1 cut(s) 487
Hpy166II GTNNAC 4 cut(s) 426, 453, 569, 649
Hpy188I TCNGA 6 cut(s) 17, 115, 136, 321, 349, 510
Hpy188III TCNNGA 5 cut(s) 80, 360, 557, 634, 743
Hpy8I GTNNAC 4 cut(s) 426, 453, 569, 649
Hpy99I CGWCG 4 cut(s) 343, 445, 503, 526
HpyAV CCTTC 7 cut(s) 81, 211, 235, 296, 347, 454, 583
HpyCH4IV ACGT 4 cut(s) 86, 434, 521, 645
HpyCH4V TGCA 4 cut(s) 268, 490, 569, 731
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 178, 200, 392
HpyF3I CTNAG 1 cut(s) 558
HpySE526I ACGT 4 cut(s) 86, 434, 521, 645
Hsp92I GRCGYC 1 cut(s) 443
Hsp92II CATG 1 cut(s) 83
Kzo9I GATC 4 cut(s) 115, 403, 688, 745
LguI GCTCTTC 3 cut(s) 295, 368, 741
LmnI GCTCC 1 cut(s) 511
Lsp1109I GCAGC 5 cut(s) 212, 252, 513, 667, 743
MaeII ACGT 4 cut(s) 86, 434, 521, 645
MalI GATC 4 cut(s) 117, 405, 690, 747
MbiI CCGCTC 2 cut(s) 13, 59
MboI GATC 4 cut(s) 115, 403, 688, 745
MflI RGATCY 3 cut(s) 115, 403, 688
MhlI GDGCHC 1 cut(s) 571
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 1 cut(s) 389
MluNI TGGCCA 1 cut(s) 395
MlyI GAGTC 7 cut(s) 95, 149, 310, 329, 562, 624, 667
MmeI TCCRAC 2 cut(s) 93, 716
Mox20I TGGCCA 1 cut(s) 395
MscI TGGCCA 1 cut(s) 395
Msp20I TGGCCA 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 178
MspI CCGG 4 cut(s) 92, 107, 416, 665
MspR9I CCNGG 2 cut(s) 108, 622
MvaI CCWGG 1 cut(s) 622
MvnI CGCG 1 cut(s) 440
MwoI GCNNNNNNNGC 4 cut(s) 50, 178, 200, 392
NciI CCSGG 1 cut(s) 108
NdeII GATC 4 cut(s) 115, 403, 688, 745
NlaIII CATG 1 cut(s) 83
NlaIV GGNNCC 3 cut(s) 117, 364, 690
NmeAIII GCCGAG 3 cut(s) 40, 229, 724
NotI GCGGCCGC 1 cut(s) 54
NspV TTCGAA 1 cut(s) 587
PagI TCATGA 1 cut(s) 79
PceI AGGCCT 1 cut(s) 620
PciSI GCTCTTC 3 cut(s) 295, 368, 741
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PfeI GAWTC 2 cut(s) 581, 593
Pfl23II CGTACG 1 cut(s) 642
PflFI GACNNNGTC 1 cut(s) 520
PleI GAGTC 7 cut(s) 95, 149, 310, 329, 561, 624, 667
PpsI GAGTC 7 cut(s) 95, 149, 310, 329, 561, 624, 667
Psp6I CCWGG 1 cut(s) 620
PspGI CCWGG 1 cut(s) 620
PspLI CGTACG 1 cut(s) 642
PspN4I GGNNCC 3 cut(s) 117, 364, 690
PspPI GGNCC 1 cut(s) 495
PstI CTGCAG 3 cut(s) 270, 492, 733
PsuI RGATCY 3 cut(s) 115, 403, 688
PsyI GACNNNGTC 1 cut(s) 520
RsaI GTAC 2 cut(s) 427, 644
RsaNI GTAC 2 cut(s) 426, 643
SapI GCTCTTC 3 cut(s) 295, 368, 741
Sau3AI GATC 4 cut(s) 115, 403, 688, 745
Sau96I GGNCC 1 cut(s) 495
SchI GAGTC 7 cut(s) 95, 149, 310, 329, 562, 624, 667
ScrFI CCNGG 2 cut(s) 108, 622
SduI GDGCHC 1 cut(s) 571
SfcI CTRYAG 5 cut(s) 266, 453, 488, 652, 729
SfuI TTCGAA 1 cut(s) 587
SmlI CTYRAG 3 cut(s) 26, 182, 632
SmoI CTYRAG 3 cut(s) 26, 182, 632
Sse9I AATT 1 cut(s) 389
SseBI AGGCCT 1 cut(s) 620
StuI AGGCCT 1 cut(s) 620
StyD4I CCNGG 2 cut(s) 106, 620
TaiI ACGT 4 cut(s) 89, 437, 524, 648
TaqI TCGA 4 cut(s) 5, 122, 338, 587
TasI AATT 1 cut(s) 389
TauI GCSGC 5 cut(s) 56, 59, 181, 448, 605
TfiI GAWTC 2 cut(s) 581, 593
TscAI CASTG 1 cut(s) 148
TseI GCWGC 5 cut(s) 200, 265, 526, 680, 731
TspDTI ATGAA 2 cut(s) 140, 272
TspRI CASTG 1 cut(s) 148
Tth111I GACNNNGTC 1 cut(s) 520
VneI GTGCAC 1 cut(s) 567
XmiI GTMKAC 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.