Rmu_sc0028451.1_g000001

Alkaline and neutral invertase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0028451.1
OG0025463
Physical Location & Seq
Forward (+)
1 .. 364
364 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0028451.1_g000001.1.cds

Sequence Viewer

Length: 364 bp
attggccaagaaagatccattgtcgccggagaagtgtacgggaacgtccgcgacgccgccgtctacagggaaggcaaagaaatcggtgaaactgaaactgcagaggcccgacgaagctccgaagcagacaacgtcgctgctgatgtcgtttcaaagtatgaggagtcctgagttgagtgcactcatcgtggaatcttcgaaggaatcttcggcggctatggtggaggaggcctgggagagactcaagaagtcgtacgtttactacagaggcaagccggtggggactcttgctgctatggatccaacggccgaggctctaaactacaatcaggtcgttcctgcagctcttcctcttgatctttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_100

Protein Analysis

120

Amino Acids

13.0

Weight (kDa)

9.21

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025463)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0028450.1_g000001 Rmu_sc0028451.1_g000001 Rmu_ssc0000368.1_g000048

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 59
AccI GTMKAC 1 cut(s) 63
AccII CGCG 1 cut(s) 51
AciI CCGC 3 cut(s) 49, 57, 213
AclWI GGATC 3 cut(s) 9, 294, 307
AcoI YGGCCR 2 cut(s) 4, 307
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 2 cut(s) 38, 255
AfiI CCNNNNNNNGG 1 cut(s) 66
AgsI TTSAA 1 cut(s) 153
AjnI CCWGG 1 cut(s) 231
AluBI AGCT 2 cut(s) 117, 345
AluI AGCT 2 cut(s) 117, 345
Alw21I GWGCWC 1 cut(s) 182
Alw26I GTCTC 1 cut(s) 233
Alw44I GTGCAC 1 cut(s) 178
AlwI GGATC 3 cut(s) 9, 294, 307
AoxI GGCC 4 cut(s) 4, 105, 229, 307
ApaLI GTGCAC 1 cut(s) 178
ApeKI GCWGC 3 cut(s) 137, 291, 342
AspS9I GGNCC 1 cut(s) 106
AsuHPI GGTGA 1 cut(s) 98
AsuII TTCGAA 1 cut(s) 198
BaeGI GKGCMC 1 cut(s) 182
BalI TGGCCA 1 cut(s) 6
BamHI GGATCC 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 182
BbvI GCAGC 3 cut(s) 124, 278, 354
BceAI ACGGC 2 cut(s) 44, 322
BcgI CGANNNNNNTGC 2 cut(s) 64, 98
BciT130I CCWGG 1 cut(s) 233
BcoDI GTCTC 1 cut(s) 233
BfmI CTRYAG 4 cut(s) 64, 99, 263, 340
BisI GCNGC 5 cut(s) 57, 138, 214, 292, 343
BlsI GCNGC 5 cut(s) 58, 139, 215, 293, 344
Bme1390I CCNGG 1 cut(s) 233
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 1 cut(s) 301
BmrFI CCNGG 1 cut(s) 233
Bpu14I TTCGAA 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 228
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 2 cut(s) 232, 310
Bsc4I CCNNNNNNNGG 1 cut(s) 66
Bse118I RCCGGY 1 cut(s) 275
BseBI CCWGG 1 cut(s) 233
BseDI CCNNGG 2 cut(s) 232, 310
BseLI CCNNNNNNNGG 1 cut(s) 66
BseMII CTCAG 1 cut(s) 160
BseRI GAGGAG 2 cut(s) 176, 240
BseSI GKGCMC 1 cut(s) 182
BseX3I CGGCCG 1 cut(s) 307
BseXI GCAGC 3 cut(s) 124, 278, 354
Bsh1236I CGCG 1 cut(s) 51
Bsh1285I CGRYCG 1 cut(s) 310
BshFI GGCC 4 cut(s) 6, 107, 231, 309
BsiEI CGRYCG 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 182
BsiSI CCGG 2 cut(s) 27, 276
BsiWI CGTACG 1 cut(s) 253
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 1 cut(s) 66
BsmAI GTCTC 1 cut(s) 233
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 4 cut(s) 6, 107, 231, 309
Bsp119I TTCGAA 1 cut(s) 198
Bsp1286I GDGCHC 1 cut(s) 182
Bsp143I GATC 3 cut(s) 14, 299, 356
BspACI CCGC 3 cut(s) 49, 57, 213
BspANI GGCC 4 cut(s) 6, 107, 231, 309
BspCNI CTCAG 1 cut(s) 161
BspFNI CGCG 1 cut(s) 51
BspLI GGNNCC 1 cut(s) 301
BspMAI CTGCAG 2 cut(s) 103, 344
BspPI GGATC 3 cut(s) 9, 294, 307
BspQI GCTCTTC 1 cut(s) 352
BspT104I TTCGAA 1 cut(s) 198
BsrFI RCCGGY 1 cut(s) 275
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 2 cut(s) 232, 310
BssMI GATC 3 cut(s) 14, 299, 356
BssNI GRCGYC 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 233
Bst6I CTCTTC 1 cut(s) 352
BstACI GRCGYC 1 cut(s) 54
BstBI TTCGAA 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 273
BstDEI CTNAG 1 cut(s) 169
BstFNI CGCG 1 cut(s) 51
BstKTI GATC 3 cut(s) 17, 302, 359
BstMAI GTCTC 1 cut(s) 233
BstMBI GATC 3 cut(s) 14, 299, 356
BstMCI CGRYCG 1 cut(s) 310
BstNI CCWGG 1 cut(s) 233
BstSCI CCNGG 1 cut(s) 231
BstSFI CTRYAG 4 cut(s) 64, 99, 263, 340
BstSLI GKGCMC 1 cut(s) 182
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 3 cut(s) 124, 278, 354
BstX2I RGATCY 2 cut(s) 14, 299
BstYI RGATCY 2 cut(s) 14, 299
BstZI CGGCCG 1 cut(s) 307
BsuRI GGCC 4 cut(s) 6, 107, 231, 309
Cac8I GCNNGC 1 cut(s) 273
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 1 cut(s) 106
CseI GACGC 1 cut(s) 62
Csp6I GTAC 2 cut(s) 37, 254
CviJI RGCY 9 cut(s) 6, 107, 117, 216, 231, 275, 309, 315, 345
CviKI_1 RGCY 9 cut(s) 6, 107, 117, 216, 231, 275, 309, 315, 345
CviQI GTAC 2 cut(s) 37, 254
DdeI CTNAG 1 cut(s) 169
DpnI GATC 3 cut(s) 16, 301, 358
DpnII GATC 3 cut(s) 14, 299, 356
DrdI GACNNNNNNGTC 1 cut(s) 59
DseDI GACNNNNNNGTC 1 cut(s) 59
EaeI YGGCCR 2 cut(s) 4, 307
EagI CGGCCG 1 cut(s) 307
Eam1104I CTCTTC 1 cut(s) 352
EarI CTCTTC 1 cut(s) 352
EclXI CGGCCG 1 cut(s) 307
Eco147I AGGCCT 1 cut(s) 231
Eco52I CGGCCG 1 cut(s) 307
EcoRII CCWGG 1 cut(s) 231
FaiI YATR 3 cut(s) 159, 219, 297
FaqI GGGAC 1 cut(s) 296
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 5 cut(s) 57, 138, 214, 292, 343
Fsp4HI GCNGC 5 cut(s) 57, 138, 214, 292, 343
GluI GCNGC 5 cut(s) 57, 138, 214, 292, 343
HaeIII GGCC 4 cut(s) 6, 107, 231, 309
HapII CCGG 2 cut(s) 27, 276
HgaI GACGC 1 cut(s) 62
Hin1I GRCGYC 1 cut(s) 54
HinfI GANTC 5 cut(s) 164, 192, 204, 241, 284
HpaII CCGG 2 cut(s) 27, 276
HphI GGTGA 1 cut(s) 98
Hpy166II GTNNAC 4 cut(s) 37, 64, 180, 260
Hpy188I TCNGA 1 cut(s) 121
Hpy188III TCNNGA 3 cut(s) 168, 245, 354
Hpy8I GTNNAC 4 cut(s) 37, 64, 180, 260
Hpy99I CGWCG 3 cut(s) 56, 114, 137
HpyAV CCTTC 2 cut(s) 65, 194
HpyCH4IV ACGT 3 cut(s) 45, 132, 256
HpyCH4V TGCA 3 cut(s) 101, 180, 342
HpyF3I CTNAG 1 cut(s) 169
HpySE526I ACGT 3 cut(s) 45, 132, 256
Hsp92I GRCGYC 1 cut(s) 54
Kzo9I GATC 3 cut(s) 14, 299, 356
LguI GCTCTTC 1 cut(s) 352
LmnI GCTCC 1 cut(s) 122
LpnPI CCDG 8 cut(s) 40, 52, 181, 218, 245, 289, 315, 352
Lsp1109I GCAGC 3 cut(s) 124, 278, 354
MaeII ACGT 3 cut(s) 45, 132, 256
MalI GATC 3 cut(s) 16, 301, 358
MboI GATC 3 cut(s) 14, 299, 356
MboII GAAGA 3 cut(s) 187, 199, 339
MflI RGATCY 2 cut(s) 14, 299
MhlI GDGCHC 1 cut(s) 182
MlsI TGGCCA 1 cut(s) 6
MluNI TGGCCA 1 cut(s) 6
MlyI GAGTC 3 cut(s) 173, 235, 278
MmeI TCCRAC 1 cut(s) 327
MnlI CCTC 7 cut(s) 97, 154, 218, 221, 261, 305, 361
Mox20I TGGCCA 1 cut(s) 6
MscI TGGCCA 1 cut(s) 6
Msp20I TGGCCA 1 cut(s) 6
MspI CCGG 2 cut(s) 27, 276
MspR9I CCNGG 1 cut(s) 233
MvaI CCWGG 1 cut(s) 233
MvnI CGCG 1 cut(s) 51
NdeII GATC 3 cut(s) 14, 299, 356
NlaIV GGNNCC 1 cut(s) 301
NmeAIII GCCGAG 1 cut(s) 335
NspV TTCGAA 1 cut(s) 198
PceI AGGCCT 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 352
PfeI GAWTC 2 cut(s) 192, 204
Pfl23II CGTACG 1 cut(s) 253
PflFI GACNNNGTC 1 cut(s) 131
PkrI GCNGC 5 cut(s) 58, 139, 215, 293, 344
PleI GAGTC 3 cut(s) 172, 235, 278
PpsI GAGTC 3 cut(s) 172, 235, 278
Psp6I CCWGG 1 cut(s) 231
PspGI CCWGG 1 cut(s) 231
PspLI CGTACG 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 301
PspPI GGNCC 1 cut(s) 106
PstI CTGCAG 2 cut(s) 103, 344
PsuI RGATCY 2 cut(s) 14, 299
PsyI GACNNNGTC 1 cut(s) 131
RsaI GTAC 2 cut(s) 38, 255
RsaNI GTAC 2 cut(s) 37, 254
SapI GCTCTTC 1 cut(s) 352
SatI GCNGC 5 cut(s) 57, 138, 214, 292, 343
Sau3AI GATC 3 cut(s) 14, 299, 356
Sau96I GGNCC 1 cut(s) 106
SchI GAGTC 3 cut(s) 173, 235, 278
ScrFI CCNGG 1 cut(s) 233
SduI GDGCHC 1 cut(s) 182
SetI ASST 6 cut(s) 48, 119, 135, 259, 334, 347
SfcI CTRYAG 4 cut(s) 64, 99, 263, 340
SfuI TTCGAA 1 cut(s) 198
SmlI CTYRAG 1 cut(s) 243
SmoI CTYRAG 1 cut(s) 243
SseBI AGGCCT 1 cut(s) 231
SsiI CCGC 3 cut(s) 49, 57, 213
StuI AGGCCT 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 231
TaiI ACGT 3 cut(s) 48, 135, 259
TaqI TCGA 1 cut(s) 198
TauI GCSGC 2 cut(s) 59, 216
TfiI GAWTC 2 cut(s) 192, 204
TseI GCWGC 3 cut(s) 137, 291, 342
Tth111I GACNNNGTC 1 cut(s) 131
VneI GTGCAC 1 cut(s) 178
XmiI GTMKAC 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.