Rmu_ssc0000116.1_g000046

exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000116.1
OG0025452
Physical Location & Seq
Forward (+)
219318 .. 219986
669 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 669 bp
atgaaaactataataaggatcaaaacactaaaatcccctgtttcaatggatgaattaagatctctcttgttaattgctgaagataagattaatcaatctttgaagtccatttctttgtctttggaggctgctatggctgcatttgatgataattccagaggtactttaccttctcattctttgaataatagtttacctaattctgaaatggtttgctcatctactacacctgctgctatcatttcaaacactaatgctcttacaagcaattcttcaaaacatattactcaatttggttgtgtgcctcaatcaactattatatctaccacacctggtgttgcttctcctattggatgcactatgcaatacgtgcctaattctgagtatatgatgagtgctgctcctgctgcattaaatggctacatcaataacccttctccttcaccatacatgttcaatggacccccaaatgacaacttatgtgctcccagtgatctaaaccgttttctagttagtgacaagtctcttggatccaagacgagcagtggtgattctgggtcaatttgtgtaggttctgttctacatcacatcatccaagaggaaggtttttgtaatatagatggttatattgatgatactaatggtaccttcatatctgattcttgttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03430 map03430
Pfam Domains
Protein Families
XPG_I XPG_N

Protein Analysis

222

Amino Acids

23.49

Weight (kDa)

4.91

Isoelectric Point (pI)

52.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025452)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0000158.1_g000020 Rmu_sc0009360.1_g000035 Rmu_ssc0000116.1_g000046

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 238
Acc36I ACCTGC 1 cut(s) 238
Acc65I GGTACC 1 cut(s) 644
AccB1I GGYRCC 1 cut(s) 644
AclWI GGATC 3 cut(s) 26, 525, 538
AcuI CTGAAG 1 cut(s) 99
AdeI CACNNNGTG 1 cut(s) 335
AfaI GTAC 2 cut(s) 163, 646
AflIII ACRYGT 1 cut(s) 450
AgsI TTSAA 6 cut(s) 45, 103, 184, 246, 276, 457
AjnI CCWGG 1 cut(s) 331
Alw21I GWGCWC 1 cut(s) 487
Alw26I GTCTC 1 cut(s) 528
AlwI GGATC 3 cut(s) 26, 525, 538
ApeKI GCWGC 5 cut(s) 128, 137, 233, 398, 407
AseI ATTAAT 1 cut(s) 90
Asp718I GGTACC 1 cut(s) 644
AspS9I GGNCC 1 cut(s) 461
AsuHPI GGTGA 2 cut(s) 435, 560
AvaII GGWCC 1 cut(s) 461
BamHI GGATCC 1 cut(s) 530
BanI GGYRCC 1 cut(s) 644
BarI GAAGNNNNNNTAC 2 cut(s) 154, 186
Bbv12I GWGCWC 1 cut(s) 487
BbvI GCAGC 5 cut(s) 115, 124, 220, 385, 394
BccI CCATC 1 cut(s) 614
BciT130I CCWGG 1 cut(s) 333
BcoDI GTCTC 1 cut(s) 528
BfaI CTAG 1 cut(s) 509
BfuAI ACCTGC 1 cut(s) 238
BglII AGATCT 1 cut(s) 59
BisI GCNGC 5 cut(s) 129, 138, 234, 399, 408
BlsI GCNGC 5 cut(s) 130, 139, 235, 400, 409
Bme1390I CCNGG 1 cut(s) 333
Bme18I GGWCC 1 cut(s) 461
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 3 cut(s) 463, 532, 646
BmrFI CCNGG 1 cut(s) 333
BmrI ACTGGG 1 cut(s) 483
BmsI GCATC 1 cut(s) 344
BmuI ACTGGG 1 cut(s) 483
BplI GAGNNNNNCTC 2 cut(s) 385, 417
BsaAI YACGTR 1 cut(s) 370
Bse1I ACTGG 1 cut(s) 489
BseBI CCWGG 1 cut(s) 333
BseGI GGATG 3 cut(s) 55, 359, 591
BseMII CTCAG 1 cut(s) 372
BseNI ACTGG 1 cut(s) 489
BseXI GCAGC 5 cut(s) 115, 124, 220, 385, 394
BshNI GGYRCC 1 cut(s) 644
BsiHKAI GWGCWC 1 cut(s) 487
BsmAI GTCTC 1 cut(s) 528
Bsp1286I GDGCHC 1 cut(s) 487
Bsp143I GATC 4 cut(s) 18, 59, 493, 530
BspCNI CTCAG 1 cut(s) 373
BspLI GGNNCC 3 cut(s) 463, 532, 646
BspMI ACCTGC 1 cut(s) 238
BspPI GGATC 3 cut(s) 26, 525, 538
BspT107I GGYRCC 1 cut(s) 644
BsrI ACTGG 1 cut(s) 489
BssMI GATC 4 cut(s) 18, 59, 493, 530
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 1 cut(s) 503
BstAPI GCANNNNNTGC 1 cut(s) 370
BstBAI YACGTR 1 cut(s) 370
BstDEI CTNAG 1 cut(s) 381
BstF5I GGATG 3 cut(s) 55, 359, 591
BstKTI GATC 4 cut(s) 21, 62, 496, 533
BstMAI GTCTC 1 cut(s) 528
BstMBI GATC 4 cut(s) 18, 59, 493, 530
BstMWI GCNNNNNNNGC 5 cut(s) 134, 137, 370, 404, 407
BstNI CCWGG 1 cut(s) 333
BstNSI RCATGY 1 cut(s) 454
BstSCI CCNGG 1 cut(s) 331
BstV1I GCAGC 5 cut(s) 115, 124, 220, 385, 394
BstX2I RGATCY 2 cut(s) 59, 530
BstYI RGATCY 2 cut(s) 59, 530
BtsCI GGATG 3 cut(s) 55, 359, 591
BtsI GCAGTG 1 cut(s) 550
BtsIMutI CAGTG 2 cut(s) 496, 550
BveI ACCTGC 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 461
CsiI ACCWGGT 1 cut(s) 331
Csp6I GTAC 2 cut(s) 162, 645
CviAII CATG 1 cut(s) 451
CviJI RGCY 3 cut(s) 128, 137, 420
CviKI_1 RGCY 3 cut(s) 128, 137, 420
CviQI GTAC 2 cut(s) 162, 645
DdeI CTNAG 1 cut(s) 381
DpnI GATC 4 cut(s) 20, 61, 495, 532
DpnII GATC 4 cut(s) 18, 59, 493, 530
DraIII CACNNNGTG 1 cut(s) 335
Eco47I GGWCC 1 cut(s) 461
Eco57I CTGAAG 1 cut(s) 99
EcoRII CCWGG 1 cut(s) 331
FaeI CATG 1 cut(s) 454
FalI AAGNNNNNCTT 2 cut(s) 256, 288
FatI CATG 1 cut(s) 450
Fnu4HI GCNGC 5 cut(s) 129, 138, 234, 399, 408
FokI GGATG 3 cut(s) 62, 366, 578
Fsp4HI GCNGC 5 cut(s) 129, 138, 234, 399, 408
FspBI CTAG 1 cut(s) 509
GluI GCNGC 5 cut(s) 129, 138, 234, 399, 408
Hin1II CATG 1 cut(s) 454
HinfI GANTC 2 cut(s) 551, 659
HphI GGTGA 2 cut(s) 435, 560
Hpy166II GTNNAC 1 cut(s) 194
Hpy188I TCNGA 3 cut(s) 205, 382, 658
Hpy188III TCNNGA 1 cut(s) 156
Hpy8I GTNNAC 1 cut(s) 194
HpyAV CCTTC 5 cut(s) 180, 444, 450, 596, 658
HpyCH4III ACNGT 1 cut(s) 503
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 4 cut(s) 140, 357, 364, 410
HpyF10VI GCNNNNNNNGC 5 cut(s) 134, 137, 370, 404, 407
HpyF3I CTNAG 1 cut(s) 381
HpySE526I ACGT 1 cut(s) 369
Hsp92II CATG 1 cut(s) 454
KpnI GGTACC 1 cut(s) 648
Kzo9I GATC 4 cut(s) 18, 59, 493, 530
LmnI GCTCC 2 cut(s) 406, 490
LpnPI CCDG 8 cut(s) 51, 169, 243, 318, 345, 417, 502, 540
Lsp1109I GCAGC 5 cut(s) 115, 124, 220, 385, 394
LweI GCATC 1 cut(s) 344
MabI ACCWGGT 1 cut(s) 331
MaeI CTAG 1 cut(s) 509
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 1 cut(s) 515
MalI GATC 4 cut(s) 20, 61, 495, 532
MboI GATC 4 cut(s) 18, 59, 493, 530
MboII GAAGA 2 cut(s) 92, 264
MflI RGATCY 2 cut(s) 59, 530
MhlI GDGCHC 1 cut(s) 487
MluCI AATT 8 cut(s) 53, 72, 151, 199, 268, 290, 376, 561
MnlI CCTC 4 cut(s) 118, 152, 315, 592
MseI TTAA 5 cut(s) 56, 71, 90, 413, 667
MspR9I CCNGG 1 cut(s) 333
MvaI CCWGG 1 cut(s) 333
MwoI GCNNNNNNNGC 5 cut(s) 134, 137, 370, 404, 407
NdeII GATC 4 cut(s) 18, 59, 493, 530
NlaIII CATG 1 cut(s) 454
NlaIV GGNNCC 3 cut(s) 463, 532, 646
NmuCI GTSAC 1 cut(s) 515
NspI RCATGY 1 cut(s) 454
PaqCI CACCTGC 1 cut(s) 238
PciI ACATGT 1 cut(s) 450
PfeI GAWTC 2 cut(s) 551, 659
PkrI GCNGC 5 cut(s) 130, 139, 235, 400, 409
Ppu21I YACGTR 1 cut(s) 370
PscI ACATGT 1 cut(s) 450
PshBI ATTAAT 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 3 cut(s) 463, 532, 646
PspPI GGNCC 1 cut(s) 461
PsrI GAACNNNNNNTAC 2 cut(s) 561, 593
PsuI RGATCY 2 cut(s) 59, 530
RsaI GTAC 2 cut(s) 163, 646
RsaNI GTAC 2 cut(s) 162, 645
SaqAI TTAA 5 cut(s) 56, 71, 90, 413, 667
SatI GCNGC 5 cut(s) 129, 138, 234, 399, 408
Sau3AI GATC 4 cut(s) 18, 59, 493, 530
Sau96I GGNCC 1 cut(s) 461
ScrFI CCNGG 1 cut(s) 333
SduI GDGCHC 1 cut(s) 487
SetI ASST 9 cut(s) 163, 172, 199, 232, 334, 372, 574, 607, 650
SexAI ACCWGGT 1 cut(s) 331
SfaNI GCATC 1 cut(s) 344
SinI GGWCC 1 cut(s) 461
Sse9I AATT 8 cut(s) 53, 72, 151, 199, 268, 290, 376, 561
SspMI CTAG 1 cut(s) 509
StyD4I CCNGG 1 cut(s) 331
TaaI ACNGT 1 cut(s) 503
TaiI ACGT 1 cut(s) 372
TasI AATT 8 cut(s) 53, 72, 151, 199, 268, 290, 376, 561
TfiI GAWTC 2 cut(s) 551, 659
Tru1I TTAA 5 cut(s) 56, 71, 90, 413, 667
Tru9I TTAA 5 cut(s) 56, 71, 90, 413, 667
TscAI CASTG 2 cut(s) 496, 550
TseFI GTSAC 1 cut(s) 515
TseI GCWGC 5 cut(s) 128, 137, 233, 398, 407
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 3 cut(s) 17, 66, 640
TspRI CASTG 2 cut(s) 496, 550
VpaK11BI GGWCC 1 cut(s) 461
VspI ATTAAT 1 cut(s) 90
XceI RCATGY 1 cut(s) 454
XspI CTAG 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.