Rmu_ssc0000117.1_g000010

Short-chain dehydrogenase reductase family 42E member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000117.1
OG0021084
Physical Location & Seq
Forward (+)
31316 .. 31962
647 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 321 bp
atgcacttactgaataaagacgaaggcatcgacggcaacatttccgtagtgaccggcgggtatggcttcgtccgattggcactctgctcggaccttctccggagacgatctagtcaagttcgagcctttgacctccgaaccacttctccttggcttgtgtatgtgagcacatacaatgttgtgattgggggaaaggagattgtgagtgggaatgaggccttaggctattcccctattgatgaacatgaggatccatgtggccgaagtaaatcaattgcagagcagttggttctcaagtacaatgctcgtcccttgaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD DUF4499

Protein Analysis

106

Amino Acids

11.73

Weight (kDa)

7.78

Isoelectric Point (pI)

55.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 51 - 106 4.9e-09 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021084)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g26170
rosa_multiflora Rmu_ssc0000117.1_g000010
rosa_rugosa Rorug07G0161500
rosa_samantha Rh7BG292900 Rh7DG302000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 111
AccIII TCCGGA 1 cut(s) 99
AciI CCGC 1 cut(s) 57
AclWI GGATC 2 cut(s) 245, 258
AcoI YGGCCR 1 cut(s) 259
AfaI GTAC 1 cut(s) 299
AgsI TTSAA 1 cut(s) 316
AloI GAACNNNNNNTCC 2 cut(s) 130, 162
Alw21I GWGCWC 1 cut(s) 170
Alw26I GTCTC 1 cut(s) 97
AlwI GGATC 2 cut(s) 245, 258
Aor13HI TCCGGA 1 cut(s) 99
AoxI GGCC 2 cut(s) 216, 259
Asp700I GAANNNNTTC 1 cut(s) 142
AspS9I GGNCC 1 cut(s) 91
AvaII GGWCC 1 cut(s) 91
AxyI CCTNAGG 1 cut(s) 220
BamHI GGATCC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 170
BceAI ACGGC 1 cut(s) 49
BcgI CGANNNNNNTGC 2 cut(s) 69, 103
BcoDI GTCTC 1 cut(s) 97
BfaI CTAG 1 cut(s) 111
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 252
BmsI GCATC 1 cut(s) 36
BpuEI CTTGAG 1 cut(s) 278
BsaJI CCNNGG 1 cut(s) 149
BsaWI WCCGGW 1 cut(s) 99
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bse118I RCCGGY 1 cut(s) 53
Bse21I CCTNAGG 1 cut(s) 220
BseAI TCCGGA 1 cut(s) 99
BseDI CCNNGG 1 cut(s) 149
BshFI GGCC 2 cut(s) 218, 261
BsiHKAI GWGCWC 1 cut(s) 170
BsiSI CCGG 2 cut(s) 54, 100
BslFI GGGAC 1 cut(s) 294
BsmAI GTCTC 1 cut(s) 97
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 294
BsnI GGCC 2 cut(s) 218, 261
Bsp1286I GDGCHC 1 cut(s) 170
Bsp13I TCCGGA 1 cut(s) 99
Bsp143I GATC 2 cut(s) 107, 250
BspACI CCGC 1 cut(s) 57
BspANI GGCC 2 cut(s) 218, 261
BspEI TCCGGA 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 252
BspPI GGATC 2 cut(s) 245, 258
BsrFI RCCGGY 1 cut(s) 53
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 149
BssMI GATC 2 cut(s) 107, 250
BssT1I CCWWGG 1 cut(s) 149
BstDEI CTNAG 1 cut(s) 220
BstKTI GATC 2 cut(s) 110, 253
BstMAI GTCTC 1 cut(s) 97
BstMBI GATC 2 cut(s) 107, 250
BstMWI GCNNNNNNNGC 2 cut(s) 33, 63
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
Bsu36I CCTNAGG 1 cut(s) 220
BsuRI GGCC 2 cut(s) 218, 261
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 1 cut(s) 91
Csp6I GTAC 1 cut(s) 298
CviAII CATG 2 cut(s) 245, 255
CviJI RGCY 6 cut(s) 66, 125, 154, 218, 225, 261
CviKI_1 RGCY 6 cut(s) 66, 125, 154, 218, 225, 261
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 1 cut(s) 220
DpnI GATC 2 cut(s) 109, 252
DpnII GATC 2 cut(s) 107, 250
DrdI GACNNNNNNGTC 1 cut(s) 111
DseDI GACNNNNNNGTC 1 cut(s) 111
EaeI YGGCCR 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 149
Eco147I AGGCCT 1 cut(s) 218
Eco47I GGWCC 1 cut(s) 91
Eco81I CCTNAGG 1 cut(s) 220
EcoT14I CCWWGG 1 cut(s) 149
ErhI CCWWGG 1 cut(s) 149
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 2 cut(s) 248, 258
FaiI YATR 5 cut(s) 63, 162, 172, 246, 256
FaqI GGGAC 1 cut(s) 294
FatI CATG 2 cut(s) 244, 254
FauI CCCGC 1 cut(s) 50
FspBI CTAG 1 cut(s) 111
HaeIII GGCC 2 cut(s) 218, 261
HapII CCGG 2 cut(s) 54, 100
Hin1II CATG 2 cut(s) 248, 258
HpaII CCGG 2 cut(s) 54, 100
Hpy188I TCNGA 3 cut(s) 74, 91, 137
Hpy188III TCNNGA 1 cut(s) 100
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 2 cut(s) 17, 104
HpyCH4V TGCA 2 cut(s) 4, 278
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 63
HpyF3I CTNAG 1 cut(s) 220
Hsp92II CATG 2 cut(s) 248, 258
Kpn2I TCCGGA 1 cut(s) 99
Kzo9I GATC 2 cut(s) 107, 250
LpnPI CCDG 2 cut(s) 67, 113
LweI GCATC 1 cut(s) 36
MaeI CTAG 1 cut(s) 111
MaeIII GTNAC 1 cut(s) 49
MalI GATC 2 cut(s) 109, 252
MboI GATC 2 cut(s) 107, 250
MfeI CAATTG 1 cut(s) 273
MflI RGATCY 1 cut(s) 250
MhlI GDGCHC 1 cut(s) 170
MluCI AATT 1 cut(s) 273
MnlI CCTC 3 cut(s) 143, 208, 241
MroI TCCGGA 1 cut(s) 99
MroXI GAANNNNTTC 1 cut(s) 142
MspI CCGG 2 cut(s) 54, 100
MunI CAATTG 1 cut(s) 273
MwoI GCNNNNNNNGC 2 cut(s) 33, 63
NdeII GATC 2 cut(s) 107, 250
NlaIII CATG 2 cut(s) 248, 258
NlaIV GGNNCC 1 cut(s) 252
NmuCI GTSAC 1 cut(s) 49
PceI AGGCCT 1 cut(s) 218
PcsI WCGNNNNNNNCGW 1 cut(s) 27
PdmI GAANNNNTTC 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 252
PspPI GGNCC 1 cut(s) 91
PsuI RGATCY 1 cut(s) 250
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
Sau3AI GATC 2 cut(s) 107, 250
Sau96I GGNCC 1 cut(s) 91
SduI GDGCHC 1 cut(s) 170
SetI ASST 2 cut(s) 96, 135
SfaNI GCATC 1 cut(s) 36
SinI GGWCC 1 cut(s) 91
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
Sse9I AATT 1 cut(s) 273
SseBI AGGCCT 1 cut(s) 218
SsiI CCGC 1 cut(s) 57
SspMI CTAG 1 cut(s) 111
StuI AGGCCT 1 cut(s) 218
StyI CCWWGG 1 cut(s) 149
TaqI TCGA 2 cut(s) 30, 121
TasI AATT 1 cut(s) 273
TatI WGTACW 1 cut(s) 297
TseFI GTSAC 1 cut(s) 49
Tsp45I GTSAC 1 cut(s) 49
TspDTI ATGAA 1 cut(s) 255
TspGWI ACGGA 1 cut(s) 34
VpaK11BI GGWCC 1 cut(s) 91
XmnI GAANNNNTTC 1 cut(s) 142
XspI CTAG 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.