Rh7DG302000

Short-chain dehydrogenase reductase family 42E member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0021084
Physical Location & Seq
Forward (+)
34699619 .. 34700736
1118 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG302000.1

Sequence Viewer

Length: 474 bp
ATGGACCTTGTCATGTACGTGTTGGATGCTTGTCTCGATTTTGGGATCAAAAGGCTTGTGTATGTTAGCACATACAATGTTGTGTTTGGGGGAAAGGAGATTGTGAGTGGGAATGAGGCCTTAGGCTATTCCCCTATTGATGAACATGAAGATCCATGTGGCCGAAGTAAATCAATTGCAGAGCAGTTGGTTCTCAAGTACAATGCTCGTCCCTTGAATGTCTCCTCAAGAGGGCATGGCTACTACAATCTCATACTGGCAGGAGAGGAAAGGACTCTAGATGGTCCTACATTAATATATGTGGATATTGTGAGGGACATGACTATACTGTTCTGTGCTGCTTACCTTCCAGAGAATGGACCGGTACCATTCTTCAGAGCTATTTTCTTCTTCCTTTTCCGTTCACTGCCGATAGTGAAGGTGGTTTTCCTTGTAGCTGCTGCAGCTCATGTTTTAGAAGATGTTTACGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD DUF4499

Protein Analysis

157

Amino Acids

17.52

Weight (kDa)

5.11

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 3 - 71 1.2e-06 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 7 - 73 6.7e-15 3-beta hydroxysteroid dehydrogenase/isomerase family
TMEM254 PF14934 107 - 157 2.2e-08 Transmembrane protein 254
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021084)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g26170
rosa_multiflora Rmu_ssc0000117.1_g000010
rosa_rugosa Rorug07G0161500
rosa_samantha Rh7BG292900 Rh7DG302000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 364
AccB1I GGYRCC 1 cut(s) 364
AccB7I CCANNNNNTGG 1 cut(s) 356
AclWI GGATC 2 cut(s) 53, 146
AcoI YGGCCR 1 cut(s) 160
AcuI CTGAAG 1 cut(s) 358
AfaI GTAC 3 cut(s) 17, 200, 366
AfiI CCNNNNNNNGG 2 cut(s) 231, 356
AflIII ACRYGT 1 cut(s) 18
AgeI ACCGGT 1 cut(s) 361
AgsI TTSAA 1 cut(s) 217
AluBI AGCT 3 cut(s) 380, 437, 446
AluI AGCT 3 cut(s) 380, 437, 446
Alw26I GTCTC 2 cut(s) 38, 226
AlwI GGATC 2 cut(s) 53, 146
AoxI GGCC 2 cut(s) 117, 160
ApeKI GCWGC 4 cut(s) 338, 437, 440, 443
AseI ATTAAT 1 cut(s) 293
AsiGI ACCGGT 1 cut(s) 361
Asp718I GGTACC 1 cut(s) 364
AspS9I GGNCC 3 cut(s) 4, 284, 359
AvaII GGWCC 3 cut(s) 4, 284, 359
AxyI CCTNAGG 1 cut(s) 121
BanI GGYRCC 1 cut(s) 364
BbvI GCAGC 4 cut(s) 325, 424, 427, 455
BccI CCATC 1 cut(s) 275
BcoDI GTCTC 2 cut(s) 38, 226
BfaI CTAG 1 cut(s) 278
BfmI CTRYAG 1 cut(s) 441
BisI GCNGC 4 cut(s) 339, 438, 441, 444
BlsI GCNGC 4 cut(s) 340, 439, 442, 445
Bme18I GGWCC 3 cut(s) 4, 284, 359
BmgT120I GGNCC 3 cut(s) 4, 284, 359
BmiI GGNNCC 1 cut(s) 366
BmsI GCATC 1 cut(s) 16
BpuEI CTTGAG 2 cut(s) 179, 211
BsaAI YACGTR 1 cut(s) 19
BsaWI WCCGGW 1 cut(s) 361
Bsc4I CCNNNNNNNGG 2 cut(s) 231, 356
Bse118I RCCGGY 1 cut(s) 361
Bse1I ACTGG 1 cut(s) 261
Bse21I CCTNAGG 1 cut(s) 121
BseGI GGATG 1 cut(s) 31
BseLI CCNNNNNNNGG 2 cut(s) 231, 356
BseNI ACTGG 1 cut(s) 261
BseRI GAGGAG 1 cut(s) 214
BseXI GCAGC 4 cut(s) 325, 424, 427, 455
BshFI GGCC 2 cut(s) 119, 162
BshNI GGYRCC 1 cut(s) 364
BshTI ACCGGT 1 cut(s) 361
BsiSI CCGG 1 cut(s) 362
BslFI GGGAC 2 cut(s) 195, 329
BslI CCNNNNNNNGG 2 cut(s) 231, 356
BsmAI GTCTC 2 cut(s) 38, 226
BsmFI GGGAC 2 cut(s) 195, 329
BsnI GGCC 2 cut(s) 119, 162
Bsp143I GATC 2 cut(s) 45, 151
BspANI GGCC 2 cut(s) 119, 162
BspLI GGNNCC 1 cut(s) 366
BspMAI CTGCAG 1 cut(s) 445
BspPI GGATC 2 cut(s) 53, 146
BspT107I GGYRCC 1 cut(s) 364
BsrFI RCCGGY 1 cut(s) 361
BsrI ACTGG 1 cut(s) 261
BssAI RCCGGY 1 cut(s) 361
BssMI GATC 2 cut(s) 45, 151
Bst4CI ACNGT 1 cut(s) 330
BstBAI YACGTR 1 cut(s) 19
BstDEI CTNAG 1 cut(s) 121
BstF5I GGATG 1 cut(s) 31
BstKTI GATC 2 cut(s) 48, 154
BstMAI GTCTC 2 cut(s) 38, 226
BstMBI GATC 2 cut(s) 45, 151
BstMWI GCNNNNNNNGC 1 cut(s) 443
BstSFI CTRYAG 1 cut(s) 441
BstV1I GCAGC 4 cut(s) 325, 424, 427, 455
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
Bsu36I CCTNAGG 1 cut(s) 121
BsuRI GGCC 2 cut(s) 119, 162
BtsCI GGATG 1 cut(s) 31
BtsI GCAGTG 1 cut(s) 404
BtsIMutI CAGTG 1 cut(s) 404
Cfr10I RCCGGY 1 cut(s) 361
Cfr13I GGNCC 3 cut(s) 4, 284, 359
Csp6I GTAC 3 cut(s) 16, 199, 365
CspAI ACCGGT 1 cut(s) 361
CviAII CATG 7 cut(s) 13, 146, 156, 236, 319, 449, 471
CviJI RGCY 8 cut(s) 55, 119, 126, 162, 240, 380, 437, 446
CviKI_1 RGCY 8 cut(s) 55, 119, 126, 162, 240, 380, 437, 446
CviQI GTAC 3 cut(s) 16, 199, 365
DdeI CTNAG 1 cut(s) 121
DpnI GATC 2 cut(s) 47, 153
DpnII GATC 2 cut(s) 45, 151
EaeI YGGCCR 1 cut(s) 160
Eco147I AGGCCT 1 cut(s) 119
Eco47I GGWCC 3 cut(s) 4, 284, 359
Eco57I CTGAAG 1 cut(s) 358
Eco81I CCTNAGG 1 cut(s) 121
FaeI CATG 7 cut(s) 16, 149, 159, 239, 322, 452, 474
FaqI GGGAC 2 cut(s) 195, 329
FatI CATG 7 cut(s) 12, 145, 155, 235, 318, 448, 470
Fnu4HI GCNGC 4 cut(s) 339, 438, 441, 444
FokI GGATG 1 cut(s) 38
Fsp4HI GCNGC 4 cut(s) 339, 438, 441, 444
FspBI CTAG 1 cut(s) 278
GluI GCNGC 4 cut(s) 339, 438, 441, 444
HaeIII GGCC 2 cut(s) 119, 162
HapII CCGG 1 cut(s) 362
Hin1II CATG 7 cut(s) 16, 149, 159, 239, 322, 452, 474
HinfI GANTC 1 cut(s) 274
HpaII CCGG 1 cut(s) 362
Hpy166II GTNNAC 2 cut(s) 404, 466
Hpy188I TCNGA 1 cut(s) 377
Hpy188III TCNNGA 4 cut(s) 35, 228, 278, 350
Hpy8I GTNNAC 2 cut(s) 404, 466
HpyAV CCTTC 2 cut(s) 356, 412
HpyCH4III ACNGT 1 cut(s) 330
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 2 cut(s) 179, 443
HpyF10VI GCNNNNNNNGC 1 cut(s) 443
HpyF3I CTNAG 1 cut(s) 121
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 7 cut(s) 16, 149, 159, 239, 322, 452, 474
KpnI GGTACC 1 cut(s) 368
Kzo9I GATC 2 cut(s) 45, 151
LpnPI CCDG 4 cut(s) 242, 246, 363, 375
Lsp1109I GCAGC 4 cut(s) 325, 424, 427, 455
LweI GCATC 1 cut(s) 16
MaeI CTAG 1 cut(s) 278
MaeII ACGT 1 cut(s) 18
MalI GATC 2 cut(s) 47, 153
MboI GATC 2 cut(s) 45, 151
MboII GAAGA 5 cut(s) 161, 364, 379, 382, 470
MfeI CAATTG 1 cut(s) 174
MflI RGATCY 1 cut(s) 151
MluCI AATT 1 cut(s) 174
MlyI GAGTC 1 cut(s) 268
MnlI CCTC 5 cut(s) 109, 224, 235, 259, 306
MseI TTAA 1 cut(s) 293
MslI CAYNNNNRTG 1 cut(s) 17
MspI CCGG 1 cut(s) 362
MunI CAATTG 1 cut(s) 174
MwoI GCNNNNNNNGC 1 cut(s) 443
NdeII GATC 2 cut(s) 45, 151
NlaIII CATG 7 cut(s) 16, 149, 159, 239, 322, 452, 474
NlaIV GGNNCC 1 cut(s) 366
PceI AGGCCT 1 cut(s) 119
PflFI GACNNNGTC 1 cut(s) 8
PflMI CCANNNNNTGG 1 cut(s) 356
PinAI ACCGGT 1 cut(s) 361
PkrI GCNGC 4 cut(s) 340, 439, 442, 445
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
Ppu21I YACGTR 1 cut(s) 19
PshBI ATTAAT 1 cut(s) 293
PspN4I GGNNCC 1 cut(s) 366
PspPI GGNCC 3 cut(s) 4, 284, 359
PstI CTGCAG 1 cut(s) 445
PsuI RGATCY 1 cut(s) 151
PsyI GACNNNGTC 1 cut(s) 8
RsaI GTAC 3 cut(s) 17, 200, 366
RsaNI GTAC 3 cut(s) 16, 199, 365
RseI CAYNNNNRTG 1 cut(s) 17
SaqAI TTAA 1 cut(s) 293
SatI GCNGC 4 cut(s) 339, 438, 441, 444
Sau3AI GATC 2 cut(s) 45, 151
Sau96I GGNCC 3 cut(s) 4, 284, 359
SchI GAGTC 1 cut(s) 268
SetI ASST 7 cut(s) 9, 21, 348, 382, 423, 439, 448
SfaNI GCATC 1 cut(s) 16
SfcI CTRYAG 1 cut(s) 441
SinI GGWCC 3 cut(s) 4, 284, 359
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 2 cut(s) 194, 226
SmoI CTYRAG 2 cut(s) 194, 226
Sse9I AATT 1 cut(s) 174
SseBI AGGCCT 1 cut(s) 119
SspMI CTAG 1 cut(s) 278
StuI AGGCCT 1 cut(s) 119
TaaI ACNGT 1 cut(s) 330
TaiI ACGT 1 cut(s) 21
TaqI TCGA 1 cut(s) 36
TasI AATT 1 cut(s) 174
TatI WGTACW 1 cut(s) 198
Tru1I TTAA 1 cut(s) 293
Tru9I TTAA 1 cut(s) 293
TscAI CASTG 1 cut(s) 411
TseI GCWGC 4 cut(s) 338, 437, 440, 443
TspDTI ATGAA 2 cut(s) 156, 162
TspGWI ACGGA 1 cut(s) 389
TspRI CASTG 1 cut(s) 411
Tth111I GACNNNGTC 1 cut(s) 8
Van91I CCANNNNNTGG 1 cut(s) 356
VpaK11BI GGWCC 3 cut(s) 4, 284, 359
VspI ATTAAT 1 cut(s) 293
XbaI TCTAGA 1 cut(s) 277
XspI CTAG 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.