Rmu_ssc0000187.1_g000021

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000187.1
OG0022685
Physical Location & Seq
Reverse (-)
109371 .. 110303
933 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 933 bp
atgagaagaaaacgactcattagattgtggatagctgaaggattcgttgaacatgtgaaaggtgtcacacagctggaagtgattgtagatagatatctttcggaactttgttttcatagcatgctacaagttgtggagaggaatgaagaaggaaggccaaaacaatgcaggatgcatgatgtcatgtgtgagcttgctctttcaacatctgagaaagaacagttttgtgctgtgtatgatgggaaagatgaagcaattaaagccggccgtttgtcagttcaatttgaaggagaaattaaatccagcacaggtatatcacagcttcgatctattcttgtctttgtcaaagagataatgttgccttctgattgtaaattttttagggtgctagacttggagaatgtgttaataactaaactgccagatgatcttgggtacttgttcaacttaagatatttaaatttgaggagaaatcgtatcaaggagcttccaaaatccataggaaagctacgcaaccttcaaacctttaacatccgcgaaagtaagatagaggaacttccaaaagaaattgccaagttgttaaatctgcgccatctaattatgtaccattacactgatgattttcttgggtttagatatgtcaaagggacaagagtaccagtggatcttagtcagatgaataaattgcaagttttgtcatgtgttgtggcagaaggagacactattaggcgggtcggttgtatgacccaacttgtaaggattggaattactaatgttaaagaaagtgatgagatgcacctatgtgacagcattcaaaagctgcagctccttcactacttgtttctaatggcaagtgatgaagaggagattcttcgaacaaatgcactatgttcatctcctcccctacttcgagtggttattttggttggctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

310

Amino Acids

35.9

Weight (kDa)

8.5

Isoelectric Point (pI)

53.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 2 - 66 7.4e-17 Disease resistance protein Winged helix domain
LRR_14 PF23598 107 - 304 7.8e-30 Leucine-rich repeat region
LRR_8 PF13855 149 - 201 7.3e-07 Leucine rich repeat
LRR_4 PF12799 149 - 186 5e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022685)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0135371
rosa_multiflora Rmu_ssc0000187.1_g000021
rosa_samantha Rh2BG381600 Rh2DG397900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 537
AciI CCGC 2 cut(s) 535, 730
AclWI GGATC 1 cut(s) 672
AcoI YGGCCR 1 cut(s) 265
AcsI RAATTY 2 cut(s) 374, 460
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 3 cut(s) 437, 605, 657
AflII CTTAAG 1 cut(s) 448
AflIII ACRYGT 1 cut(s) 52
AgsI TTSAA 7 cut(s) 50, 204, 281, 287, 445, 521, 815
AleI CACNNNNGTG 1 cut(s) 801
AluBI AGCT 8 cut(s) 35, 73, 193, 322, 487, 508, 820, 826
AluI AGCT 8 cut(s) 35, 73, 193, 322, 487, 508, 820, 826
Alw26I GTCTC 1 cut(s) 711
AlwI GGATC 1 cut(s) 672
AoxI GGCC 2 cut(s) 155, 265
ApeKI GCWGC 2 cut(s) 820, 823
ApoI RAATTY 2 cut(s) 374, 460
AspLEI GCGC 1 cut(s) 591
AsuII TTCGAA 1 cut(s) 874
BaeI ACNNNNGTAYC 2 cut(s) 639, 672
BbvI GCAGC 2 cut(s) 807, 835
BccI CCATC 2 cut(s) 233, 600
BceAI ACGGC 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 711
BfaI CTAG 1 cut(s) 389
BfmI CTRYAG 1 cut(s) 821
BfrI CTTAAG 1 cut(s) 448
BisI GCNGC 2 cut(s) 821, 824
BlsI GCNGC 2 cut(s) 822, 825
BmsI GCATC 2 cut(s) 162, 783
Bpu14I TTCGAA 1 cut(s) 874
BsaBI GATNNNNATC 1 cut(s) 93
BsaXI ACNNNNNCTCC 4 cut(s) 389, 419, 460, 490
Bse118I RCCGGY 1 cut(s) 263
Bse1I ACTGG 1 cut(s) 659
Bse8I GATNNNNATC 1 cut(s) 93
BseGI GGATG 2 cut(s) 177, 531
BseJI GATNNNNATC 1 cut(s) 93
BseMII CTCAG 1 cut(s) 201
BseNI ACTGG 1 cut(s) 659
BseRI GAGGAG 3 cut(s) 481, 878, 888
BseX3I CGGCCG 1 cut(s) 265
BseXI GCAGC 2 cut(s) 807, 835
Bsh1236I CGCG 1 cut(s) 537
Bsh1285I CGRYCG 1 cut(s) 268
BshFI GGCC 2 cut(s) 157, 267
BsiEI CGRYCG 1 cut(s) 268
BsiSI CCGG 1 cut(s) 264
BslFI GGGAC 1 cut(s) 661
BsmAI GTCTC 1 cut(s) 711
BsmFI GGGAC 1 cut(s) 661
BsmI GAATGC 1 cut(s) 810
BsnI GGCC 2 cut(s) 157, 267
Bsp119I TTCGAA 1 cut(s) 874
Bsp143I GATC 3 cut(s) 326, 427, 664
BspACI CCGC 2 cut(s) 535, 730
BspANI GGCC 2 cut(s) 157, 267
BspCNI CTCAG 1 cut(s) 202
BspFNI CGCG 1 cut(s) 537
BspMAI CTGCAG 1 cut(s) 825
BspPI GGATC 1 cut(s) 672
BspT104I TTCGAA 1 cut(s) 874
BspTI CTTAAG 1 cut(s) 448
BsrFI RCCGGY 1 cut(s) 263
BsrI ACTGG 1 cut(s) 659
BssAI RCCGGY 1 cut(s) 263
BssMI GATC 3 cut(s) 326, 427, 664
Bst4CI ACNGT 1 cut(s) 222
Bst6I CTCTTC 1 cut(s) 855
BstAFI CTTAAG 1 cut(s) 448
BstBI TTCGAA 1 cut(s) 874
BstC8I GCNNGC 3 cut(s) 122, 195, 265
BstDEI CTNAG 2 cut(s) 210, 668
BstF5I GGATG 2 cut(s) 177, 531
BstFNI CGCG 1 cut(s) 537
BstHHI GCGC 1 cut(s) 591
BstKTI GATC 3 cut(s) 329, 430, 667
BstMAI GTCTC 1 cut(s) 711
BstMBI GATC 3 cut(s) 326, 427, 664
BstMCI CGRYCG 1 cut(s) 268
BstMWI GCNNNNNNNGC 1 cut(s) 260
BstNSI RCATGY 2 cut(s) 56, 124
BstSFI CTRYAG 1 cut(s) 821
BstUI CGCG 1 cut(s) 537
BstV1I GCAGC 2 cut(s) 807, 835
BstX2I RGATCY 1 cut(s) 664
BstYI RGATCY 1 cut(s) 664
BstZI CGGCCG 1 cut(s) 265
BsuRI GGCC 2 cut(s) 157, 267
BtsCI GGATG 2 cut(s) 177, 531
BtsIMutI CAGTG 2 cut(s) 612, 666
Cac8I GCNNGC 3 cut(s) 122, 195, 265
CfoI GCGC 1 cut(s) 591
Cfr10I RCCGGY 1 cut(s) 263
Csp6I GTAC 3 cut(s) 436, 604, 656
CviAII CATG 5 cut(s) 53, 121, 176, 184, 699
CviQI GTAC 3 cut(s) 436, 604, 656
DdeI CTNAG 2 cut(s) 210, 668
DpnI GATC 3 cut(s) 328, 429, 666
DpnII GATC 3 cut(s) 326, 427, 664
DraI TTTAAA 1 cut(s) 459
EaeI YGGCCR 1 cut(s) 265
EagI CGGCCG 1 cut(s) 265
Eam1104I CTCTTC 1 cut(s) 855
EarI CTCTTC 1 cut(s) 855
EclXI CGGCCG 1 cut(s) 265
Eco32I GATATC 1 cut(s) 95
Eco52I CGGCCG 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 57
EcoRV GATATC 1 cut(s) 95
EcoT22I ATGCAT 1 cut(s) 177
FaeI CATG 5 cut(s) 56, 124, 179, 187, 702
FaqI GGGAC 1 cut(s) 661
FatI CATG 5 cut(s) 52, 120, 175, 183, 698
FauI CCCGC 1 cut(s) 723
Fnu4HI GCNGC 2 cut(s) 821, 824
FokI GGATG 2 cut(s) 184, 518
Fsp4HI GCNGC 2 cut(s) 821, 824
FspBI CTAG 1 cut(s) 389
GlaI GCGC 1 cut(s) 590
GluI GCNGC 2 cut(s) 821, 824
HaeIII GGCC 2 cut(s) 157, 267
HapII CCGG 1 cut(s) 264
HhaI GCGC 1 cut(s) 591
Hin1II CATG 5 cut(s) 56, 124, 179, 187, 702
Hin6I GCGC 1 cut(s) 589
HinP1I GCGC 1 cut(s) 589
HinfI GANTC 3 cut(s) 15, 42, 868
HpaII CCGG 1 cut(s) 264
Hpy188I TCNGA 4 cut(s) 103, 211, 367, 675
HpyAV CCTTC 8 cut(s) 32, 143, 147, 281, 372, 527, 707, 839
HpyCH4III ACNGT 1 cut(s) 222
HpyCH4V TGCA 6 cut(s) 168, 175, 688, 796, 823, 884
HpyF10VI GCNNNNNNNGC 1 cut(s) 260
HpyF3I CTNAG 2 cut(s) 210, 668
Hsp92II CATG 5 cut(s) 56, 124, 179, 187, 702
HspAI GCGC 1 cut(s) 589
KroI GCCGGC 1 cut(s) 263
KroNI GCCGGC 1 cut(s) 265
Kzo9I GATC 3 cut(s) 326, 427, 664
LmnI GCTCC 2 cut(s) 484, 831
LpnPI CCDG 7 cut(s) 59, 154, 277, 294, 316, 435, 672
Lsp1109I GCAGC 2 cut(s) 807, 835
LweI GCATC 2 cut(s) 162, 783
MaeI CTAG 1 cut(s) 389
MaeIII GTNAC 2 cut(s) 64, 803
MalI GATC 3 cut(s) 328, 429, 666
MboI GATC 3 cut(s) 326, 427, 664
MboII GAAGA 4 cut(s) 18, 158, 863, 872
MflI RGATCY 1 cut(s) 664
MluCI AATT 9 cut(s) 255, 281, 294, 374, 460, 567, 597, 683, 765
MlyI GAGTC 1 cut(s) 9
MnlI CCTC 5 cut(s) 132, 459, 544, 856, 909
Mph1103I ATGCAT 1 cut(s) 177
MroNI GCCGGC 1 cut(s) 263
MseI TTAA 8 cut(s) 258, 297, 407, 449, 458, 528, 581, 777
MslI CAYNNNNRTG 1 cut(s) 801
MspA1I CMGCKG 1 cut(s) 73
MspCI CTTAAG 1 cut(s) 448
MspI CCGG 1 cut(s) 264
Mva1269I GAATGC 1 cut(s) 810
MvnI CGCG 1 cut(s) 537
MwoI GCNNNNNNNGC 1 cut(s) 260
NaeI GCCGGC 1 cut(s) 265
NdeII GATC 3 cut(s) 326, 427, 664
NgoMIV GCCGGC 1 cut(s) 263
NlaIII CATG 5 cut(s) 56, 124, 179, 187, 702
NmuCI GTSAC 2 cut(s) 64, 803
NsiI ATGCAT 1 cut(s) 177
NspI RCATGY 2 cut(s) 56, 124
NspV TTCGAA 1 cut(s) 874
OliI CACNNNNGTG 1 cut(s) 801
PaeI GCATGC 1 cut(s) 124
PciI ACATGT 1 cut(s) 52
PctI GAATGC 1 cut(s) 810
PdiI GCCGGC 1 cut(s) 265
PfeI GAWTC 2 cut(s) 42, 868
PkrI GCNGC 2 cut(s) 822, 825
PleI GAGTC 1 cut(s) 9
PpsI GAGTC 1 cut(s) 9
PscI ACATGT 1 cut(s) 52
PstI CTGCAG 1 cut(s) 825
PsuI RGATCY 1 cut(s) 664
PvuII CAGCTG 1 cut(s) 73
RsaI GTAC 3 cut(s) 437, 605, 657
RsaNI GTAC 3 cut(s) 436, 604, 656
RseI CAYNNNNRTG 1 cut(s) 801
SaqAI TTAA 8 cut(s) 258, 297, 407, 449, 458, 528, 581, 777
SatI GCNGC 2 cut(s) 821, 824
Sau3AI GATC 3 cut(s) 326, 427, 664
SchI GAGTC 1 cut(s) 9
SfaNI GCATC 2 cut(s) 162, 783
SfcI CTRYAG 1 cut(s) 821
SfuI TTCGAA 1 cut(s) 874
SmiI ATTTAAAT 1 cut(s) 459
SmiMI CAYNNNNRTG 1 cut(s) 801
SmlI CTYRAG 1 cut(s) 448
SmoI CTYRAG 1 cut(s) 448
SphI GCATGC 1 cut(s) 124
Sse9I AATT 9 cut(s) 255, 281, 294, 374, 460, 567, 597, 683, 765
SsiI CCGC 2 cut(s) 535, 730
SspMI CTAG 1 cut(s) 389
SwaI ATTTAAAT 1 cut(s) 459
TaaI ACNGT 1 cut(s) 222
TaqI TCGA 3 cut(s) 325, 874, 910
TasI AATT 9 cut(s) 255, 281, 294, 374, 460, 567, 597, 683, 765
TfiI GAWTC 2 cut(s) 42, 868
Tru1I TTAA 8 cut(s) 258, 297, 407, 449, 458, 528, 581, 777
Tru9I TTAA 8 cut(s) 258, 297, 407, 449, 458, 528, 581, 777
TscAI CASTG 2 cut(s) 619, 666
TseFI GTSAC 2 cut(s) 64, 803
TseI GCWGC 2 cut(s) 820, 823
Tsp45I GTSAC 2 cut(s) 64, 803
TspDTI ATGAA 6 cut(s) 104, 159, 264, 692, 873, 882
TspRI CASTG 2 cut(s) 619, 666
Vha464I CTTAAG 1 cut(s) 448
XapI RAATTY 2 cut(s) 374, 460
XceI RCATGY 2 cut(s) 56, 124
XspI CTAG 1 cut(s) 389
Zsp2I ATGCAT 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.