Rroxscaffold_1G00014240

Transcription elongation factor B polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0017206
Physical Location & Seq
Reverse (-)
17554303 .. 17555657
1355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00014240.1

Sequence Viewer

Length: 297 bp
ATGAAGAAAGAAGACACCGTGAAGCTGATCAGCGCCGAGGGCTTCGAATTCGTCGTCCACAAAGACGCCGCCATGGTCTCTCAGACCATCCGCAACATGCTCACATCTCCTGGGGGTTTTGCTGAAACGGAACACGGTGAGGTGACGTTCCCTGAGATCAGCACCACCATTCTTGAGAAGATCTGCAAGTATTTTTATTGGAATCTTCAATTCGCCAGTGGTAAGCAGACTGAGTTTCACATTGAACCAGAACTGACCCTGGAGTTGATGATGGCAGCCAATTATCTTCATACATAG

Protein Analysis

98

Amino Acids

11.19

Weight (kDa)

5.08

Isoelectric Point (pI)

49.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Skp1_POZ PF03931 6 - 67 7.5e-08 Skp1 family, tetramerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017206)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59140
fragaria_vesca FvH4_3g37160
malus_domestica MD03G1084600.v1.1 MD11G1092100.v1.1
prunus_persica Prupe.6G067900_v2.0.a1
pyrus_communis pycom03g06680 pycom11g07740
rosa_chinensis RchiOBHm_Chr5g0066881
rosa_multiflora Rmu_sc0000605.1_g000059
rosa_roxburghii Rroxscaffold_1G00014240
rosa_rugosa Rorug05G0379200
rosa_samantha Rh5CG477900
rosa_wichuraiana Rw5G041050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 69, 91
AcsI RAATTY 1 cut(s) 47
AcyI GRCGYC 1 cut(s) 66
AgsI TTSAA 2 cut(s) 209, 245
AjnI CCWGG 2 cut(s) 109, 258
AluBI AGCT 1 cut(s) 25
AluI AGCT 1 cut(s) 25
Alw26I GTCTC 1 cut(s) 82
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 1 cut(s) 47
ArsI GACNNNNNNTTYG 2 cut(s) 39, 71
AspLEI GCGC 1 cut(s) 35
AsuHPI GGTGA 2 cut(s) 149, 154
AsuII TTCGAA 1 cut(s) 45
BbsI GAAGAC 1 cut(s) 18
BbvI GCAGC 1 cut(s) 287
BccI CCATC 2 cut(s) 95, 265
BciT130I CCWGG 2 cut(s) 111, 260
BclI TGATCA 1 cut(s) 27
BcoDI GTCTC 1 cut(s) 82
BfoI RGCGCY 1 cut(s) 36
BglII AGATCT 1 cut(s) 180
BisI GCNGC 2 cut(s) 69, 276
BlsI GCNGC 2 cut(s) 70, 277
Bme1390I CCNGG 2 cut(s) 111, 260
BmrFI CCNGG 2 cut(s) 111, 260
BpiI GAAGAC 1 cut(s) 18
BpmI CTGGAG 1 cut(s) 281
Bpu14I TTCGAA 1 cut(s) 45
BpuEI CTTGAG 1 cut(s) 194
BsaHI GRCGYC 1 cut(s) 66
BsaI GGTCTC 1 cut(s) 82
BsaJI CCNNGG 4 cut(s) 36, 72, 110, 258
Bse1I ACTGG 1 cut(s) 216
BseBI CCWGG 2 cut(s) 111, 260
BseDI CCNNGG 4 cut(s) 36, 72, 110, 258
BseGI GGATG 1 cut(s) 87
BseMII CTCAG 3 cut(s) 95, 144, 222
BseNI ACTGG 1 cut(s) 216
BseXI GCAGC 1 cut(s) 287
BsmAI GTCTC 1 cut(s) 82
Bso31I GGTCTC 1 cut(s) 82
Bsp119I TTCGAA 1 cut(s) 45
Bsp143I GATC 3 cut(s) 27, 156, 180
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 2 cut(s) 69, 91
BspCNI CTCAG 3 cut(s) 94, 145, 223
BspT104I TTCGAA 1 cut(s) 45
BspTNI GGTCTC 1 cut(s) 82
BsrI ACTGG 1 cut(s) 216
BssECI CCNNGG 4 cut(s) 36, 72, 110, 258
BssMI GATC 3 cut(s) 27, 156, 180
BssNI GRCGYC 1 cut(s) 66
BssT1I CCWWGG 1 cut(s) 72
Bst2UI CCWGG 2 cut(s) 111, 260
Bst4CI ACNGT 2 cut(s) 19, 137
BstACI GRCGYC 1 cut(s) 66
BstBI TTCGAA 1 cut(s) 45
BstDEI CTNAG 3 cut(s) 81, 153, 231
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 1 cut(s) 87
BstH2I RGCGCY 1 cut(s) 36
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 3 cut(s) 30, 159, 183
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 3 cut(s) 27, 156, 180
BstMWI GCNNNNNNNGC 1 cut(s) 39
BstNI CCWGG 2 cut(s) 111, 260
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 2 cut(s) 109, 258
BstV1I GCAGC 1 cut(s) 287
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 223
CfoI GCGC 1 cut(s) 35
CseI GACGC 1 cut(s) 74
CspCI CAANNNNNGTGG 2 cut(s) 154, 189
CviAII CATG 2 cut(s) 73, 97
CviJI RGCY 3 cut(s) 25, 42, 278
CviKI_1 RGCY 3 cut(s) 25, 42, 278
DdeI CTNAG 3 cut(s) 81, 153, 231
DpnI GATC 3 cut(s) 29, 158, 182
DpnII GATC 3 cut(s) 27, 156, 180
Eco130I CCWWGG 1 cut(s) 72
Eco31I GGTCTC 1 cut(s) 82
EcoRI GAATTC 1 cut(s) 47
EcoRII CCWGG 2 cut(s) 109, 258
EcoT14I CCWWGG 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 2 cut(s) 76, 100
FaiI YATR 4 cut(s) 74, 98, 291, 295
FatI CATG 2 cut(s) 72, 96
FbaI TGATCA 1 cut(s) 27
Fnu4HI GCNGC 2 cut(s) 69, 276
FokI GGATG 1 cut(s) 74
Fsp4HI GCNGC 2 cut(s) 69, 276
GlaI GCGC 1 cut(s) 34
GluI GCNGC 2 cut(s) 69, 276
GsuI CTGGAG 1 cut(s) 281
HaeII RGCGCY 1 cut(s) 36
HgaI GACGC 1 cut(s) 74
HhaI GCGC 1 cut(s) 35
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 2 cut(s) 76, 100
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HinfI GANTC 1 cut(s) 202
HphI GGTGA 2 cut(s) 149, 154
Hpy166II GTNNAC 1 cut(s) 58
Hpy188I TCNGA 1 cut(s) 84
Hpy188III TCNNGA 1 cut(s) 173
Hpy8I GTNNAC 1 cut(s) 58
Hpy99I CGWCG 1 cut(s) 56
HpyCH4III ACNGT 2 cut(s) 19, 137
HpyCH4IV ACGT 1 cut(s) 146
HpyCH4V TGCA 1 cut(s) 186
HpyF10VI GCNNNNNNNGC 1 cut(s) 39
HpyF3I CTNAG 3 cut(s) 81, 153, 231
HpySE526I ACGT 1 cut(s) 146
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 2 cut(s) 76, 100
HspAI GCGC 1 cut(s) 33
Ksp22I TGATCA 1 cut(s) 27
Kzo9I GATC 3 cut(s) 27, 156, 180
LpnPI CCDG 7 cut(s) 96, 123, 165, 229, 245, 261, 272
Lsp1109I GCAGC 1 cut(s) 287
MaeII ACGT 1 cut(s) 146
MaeIII GTNAC 1 cut(s) 142
MalI GATC 3 cut(s) 29, 158, 182
MboI GATC 3 cut(s) 27, 156, 180
MboII GAAGA 5 cut(s) 16, 23, 190, 197, 278
MflI RGATCY 1 cut(s) 180
MluCI AATT 3 cut(s) 47, 209, 280
MnlI CCTC 2 cut(s) 31, 133
MspR9I CCNGG 2 cut(s) 111, 260
MvaI CCWGG 2 cut(s) 111, 260
MwoI GCNNNNNNNGC 1 cut(s) 39
NcoI CCATGG 1 cut(s) 72
NdeII GATC 3 cut(s) 27, 156, 180
NlaIII CATG 2 cut(s) 76, 100
NmeAIII GCCGAG 1 cut(s) 61
NmuCI GTSAC 1 cut(s) 142
NspI RCATGY 1 cut(s) 100
NspV TTCGAA 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PfeI GAWTC 1 cut(s) 202
PkrI GCNGC 2 cut(s) 70, 277
Psp6I CCWGG 2 cut(s) 109, 258
PspGI CCWGG 2 cut(s) 109, 258
PsuI RGATCY 1 cut(s) 180
SatI GCNGC 2 cut(s) 69, 276
Sau3AI GATC 3 cut(s) 27, 156, 180
ScrFI CCNGG 2 cut(s) 111, 260
SetI ASST 3 cut(s) 27, 144, 149
SfuI TTCGAA 1 cut(s) 45
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
Sse9I AATT 3 cut(s) 47, 209, 280
SsiI CCGC 2 cut(s) 69, 91
StyD4I CCNGG 2 cut(s) 109, 258
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 2 cut(s) 19, 137
TaiI ACGT 1 cut(s) 149
TaqI TCGA 1 cut(s) 45
TasI AATT 3 cut(s) 47, 209, 280
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 1 cut(s) 202
TscAI CASTG 1 cut(s) 223
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 1 cut(s) 275
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 2 cut(s) 17, 278
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 1 cut(s) 223
XapI RAATTY 1 cut(s) 47
XceI RCATGY 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.