Rroxscaffold_1G00028560

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0017107
Physical Location & Seq
Forward (+)
36387721 .. 36389091
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00028560.1

Sequence Viewer

Length: 1371 bp
ATGCATCATGAGTGGGATGAAAATATCCAACCTGAAACCCTAGTCTCCCACGATCAGGAACAAAGGCCACTATCCGAAATGTGGAAGGAAATTCAAGGCCTTAATAACTGGGAAGGTCTTCTAGACCCAACATTCAATCCCCATCTCCGACAAGAAATCATCCGATATGGTGAATTCGCCCAAGCCTCTTACGATTCCTTTGACTTCGACCCTCACTCCAAGTACTGTGGCACGTGTAAATACCAAGGTGCTCATTTCTTCGAAAACCTGGACATGGCAGATCGAGGCTACCAAATCAGCCGATATCTATATGCAACTTCCAACATCAATCTCCCCAATTTCTTCCAAAAGTCGAAGATCAGTAGCATGTGGAGCACCCATGCAAACTGGATGGGCTACGTGGCCGTAGCCACAGACGTCGATGAAATCAAAAGACTCGGTCGAAGAGACATAGTGATTGCATGGAGAGGAACAGTCACATACCTTGAATGGATTTACGACCTCAAAGACATTCTCCGGCCAGCTTATTTTGGCAATGATCCCTCAGTGAAAATCGAGTCAGGGTTCCATGAGTTATACACCAAGAAAGAAGACGCATGCAAATACTGCTCATTTTCTGCCCGTGAACAAGTTCTAGCAGAGATCAAGAGGCTCCGTGAACGCTACAAAGGAGAGGAGATCAGTATTACAATCACAGGTCATAGTCTTGGGGCTGCTTTGGCTATCTTGAGTGCCTATGATATTGCAGAAATGGGACTTGATATTGTGCATGATGAGGGTAGGTTGGAAACTAAAATTCCAATCACTGTGTACTCATTTTCGGGTCCTAGAGTGGGAAATCTTAAGTTCAAGGAAAGATGTGATGAGCTTGGAGTTCAGGTGCTGAGAGTTATCAATGTGCATGATAAGGTGCCTACAGTGCCTGGTATCATTACGAATGAGAAGTTTAGGTATCAAAAGTACATAGAAGACACTATTTCATTTCCTTGGAGTTATGCTCATCTTGGAGTGGAGCTTGCACTGGATCACACCCAAAGCCCATTTCTCAAGCCGACTAATGATTTTGCGTGTGCACACAATCTTGAAGCTCTTTTGCATTTGGTTGACGGGTACCACGGGAAGGGCAAAAGGTTCTGCTTGGTTGCCAAGAGGGATATTGCACTTGTGAACAAGAGCTGTGATTTTTTAAAGAACCAATATGGTGTGCCACCACACTGGCGGCAAGACGAGAATAAGGGTATGGTGAGGAATAGGGATGGGAGGTGGGTTCTTCCCGAGAGGCCCAAAGTTGAAGCTCACCCACCCGATACAGTTCACCACCTTGAGCAAGTGCTGAACAAATCATCTAGCTCTCAATTTGAAATGCCTTGA

Protein Analysis

456

Amino Acids

52.73

Weight (kDa)

6.14

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 152 - 310 3.6e-37 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017107)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51440
prunus_persica Prupe.4G253800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0052751
rosa_laevigata RLG00000034801
rosa_multiflora Rmu_sc0010265.1_g000002 Rmu_sc0021934.1_g000012
rosa_roxburghii Rroxscaffold_1G00028560
rosa_rugosa Rorug05G0270200
rosa_samantha Rh5AG342200 Rh5BG352900 Rh5CG379400 Rh5DG369200
rosa_wichuraiana Rw5G032260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 420
Acc65I GGTACC 1 cut(s) 1110
AccB1I GGYRCC 2 cut(s) 910, 1110
AciI CCGC 1 cut(s) 1219
AclWI GGATC 2 cut(s) 533, 1032
AcoI YGGCCR 2 cut(s) 402, 518
AcsI RAATTY 3 cut(s) 90, 173, 795
AcvI CACGTG 1 cut(s) 234
AcyI GRCGYC 1 cut(s) 417
AfaI GTAC 4 cut(s) 224, 812, 962, 1112
AfiI CCNNNNNNNGG 5 cut(s) 55, 81, 274, 833, 1120
AflII CTTAAG 1 cut(s) 842
AflIII ACRYGT 1 cut(s) 233
AgsI TTSAA 7 cut(s) 95, 136, 488, 850, 1085, 1292, 1361
AjnI CCWGG 2 cut(s) 267, 922
AjuI GAANNNNNNNTTGG 2 cut(s) 1026, 1058
AluBI AGCT 7 cut(s) 524, 868, 1015, 1088, 1176, 1295, 1350
AluI AGCT 7 cut(s) 524, 868, 1015, 1088, 1176, 1295, 1350
Alw21I GWGCWC 3 cut(s) 253, 377, 1075
Alw26I GTCTC 2 cut(s) 49, 441
Alw44I GTGCAC 1 cut(s) 1071
AlwI GGATC 2 cut(s) 533, 1032
AlwNI CAGNNNCTG 2 cut(s) 883, 923
Ama87I CYCGRG 1 cut(s) 1274
AoxI GGCC 5 cut(s) 65, 97, 402, 518, 1280
ApaLI GTGCAC 1 cut(s) 1071
ApeKI GCWGC 1 cut(s) 713
ApoI RAATTY 3 cut(s) 90, 173, 795
Asp700I GAANNNNTTC 2 cut(s) 117, 630
Asp718I GGTACC 1 cut(s) 1110
AspS9I GGNCC 2 cut(s) 824, 1281
AsuHPI GGTGA 4 cut(s) 182, 1255, 1289, 1307
AsuII TTCGAA 1 cut(s) 261
AvaI CYCGRG 1 cut(s) 1274
AvaII GGWCC 1 cut(s) 824
BaeGI GKGCMC 1 cut(s) 1075
BanI GGYRCC 2 cut(s) 910, 1110
BbrPI CACGTG 1 cut(s) 234
BbsI GAAGAC 3 cut(s) 110, 597, 975
Bbv12I GWGCWC 3 cut(s) 253, 377, 1075
BbvI GCAGC 1 cut(s) 700
BccI CCATC 3 cut(s) 150, 385, 1250
BceAI ACGGC 1 cut(s) 389
BciT130I CCWGG 2 cut(s) 269, 924
BcoDI GTCTC 2 cut(s) 49, 441
BfaI CTAG 5 cut(s) 41, 122, 635, 828, 1347
BfmI CTRYAG 1 cut(s) 915
BfrI CTTAAG 1 cut(s) 842
BisI GCNGC 2 cut(s) 714, 1220
BlsI GCNGC 2 cut(s) 715, 1221
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 2 cut(s) 269, 924
Bme18I GGWCC 1 cut(s) 824
BmeT110I CYCGRG 1 cut(s) 1274
BmgT120I GGNCC 2 cut(s) 824, 1281
BmiI GGNNCC 5 cut(s) 566, 653, 825, 912, 1112
BmrFI CCNGG 2 cut(s) 269, 924
BmrI ACTGGG 1 cut(s) 118
BmsI GCATC 1 cut(s) 13
BmuI ACTGGG 1 cut(s) 118
BpiI GAAGAC 3 cut(s) 110, 597, 975
BplI GAGNNNNNCTC 2 cut(s) 982, 1014
Bpu14I TTCGAA 1 cut(s) 261
BpuEI CTTGAG 3 cut(s) 748, 1031, 1343
BsaAI YACGTR 2 cut(s) 234, 400
BsaHI GRCGYC 1 cut(s) 417
BsaJI CCNNGG 3 cut(s) 244, 986, 1114
BsaXI ACNNNNNCTCC 4 cut(s) 200, 230, 864, 894
Bsc4I CCNNNNNNNGG 5 cut(s) 55, 81, 274, 833, 1120
Bse1I ACTGG 4 cut(s) 113, 392, 1026, 1220
Bse3DI GCAATG 1 cut(s) 541
BseBI CCWGG 2 cut(s) 269, 924
BseDI CCNNGG 3 cut(s) 244, 986, 1114
BseGI GGATG 4 cut(s) 22, 159, 396, 1261
BseLI CCNNNNNNNGG 5 cut(s) 55, 81, 274, 833, 1120
BseMI GCAATG 1 cut(s) 541
BseMII CTCAG 2 cut(s) 558, 875
BseNI ACTGG 4 cut(s) 113, 392, 1026, 1220
BseRI GAGGAG 1 cut(s) 689
BseSI GKGCMC 1 cut(s) 1075
BseXI GCAGC 1 cut(s) 700
Bsh1285I CGRYCG 1 cut(s) 442
BshFI GGCC 5 cut(s) 67, 99, 404, 520, 1282
BshNI GGYRCC 2 cut(s) 910, 1110
BsiEI CGRYCG 1 cut(s) 442
BsiHKAI GWGCWC 3 cut(s) 253, 377, 1075
BsiHKCI CYCGRG 1 cut(s) 1274
BsiSI CCGG 1 cut(s) 517
BslFI GGGAC 1 cut(s) 768
BslI CCNNNNNNNGG 5 cut(s) 55, 81, 274, 833, 1120
BsmAI GTCTC 2 cut(s) 49, 441
BsmFI GGGAC 1 cut(s) 768
BsnI GGCC 5 cut(s) 67, 99, 404, 520, 1282
BsoBI CYCGRG 1 cut(s) 1274
Bsp119I TTCGAA 1 cut(s) 261
Bsp1286I GDGCHC 3 cut(s) 253, 377, 1075
Bsp143I GATC 7 cut(s) 52, 280, 357, 538, 642, 678, 1024
BspACI CCGC 1 cut(s) 1219
BspANI GGCC 5 cut(s) 67, 99, 404, 520, 1282
BspCNI CTCAG 2 cut(s) 557, 876
BspHI TCATGA 1 cut(s) 7
BspLI GGNNCC 5 cut(s) 566, 653, 825, 912, 1112
BspPI GGATC 2 cut(s) 533, 1032
BspT104I TTCGAA 1 cut(s) 261
BspT107I GGYRCC 2 cut(s) 910, 1110
BspTI CTTAAG 1 cut(s) 842
BsrDI GCAATG 1 cut(s) 541
BsrI ACTGG 4 cut(s) 113, 392, 1026, 1220
BssECI CCNNGG 3 cut(s) 244, 986, 1114
BssMI GATC 7 cut(s) 52, 280, 357, 538, 642, 678, 1024
BssNI GRCGYC 1 cut(s) 417
BssT1I CCWWGG 2 cut(s) 244, 986
Bst2UI CCWGG 2 cut(s) 269, 924
Bst4CI ACNGT 5 cut(s) 227, 475, 808, 919, 1312
Bst6I CTCTTC 1 cut(s) 439
BstACI GRCGYC 1 cut(s) 417
BstAFI CTTAAG 1 cut(s) 842
BstAPI GCANNNNNTGC 1 cut(s) 606
BstBAI YACGTR 2 cut(s) 234, 400
BstBI TTCGAA 1 cut(s) 261
BstC8I GCNNGC 3 cut(s) 522, 598, 1017
BstDEI CTNAG 2 cut(s) 544, 884
BstDSI CCRYGG 1 cut(s) 1114
BstF5I GGATG 4 cut(s) 22, 159, 396, 1261
BstKTI GATC 7 cut(s) 55, 283, 360, 541, 645, 681, 1027
BstMAI GTCTC 2 cut(s) 49, 441
BstMBI GATC 7 cut(s) 52, 280, 357, 538, 642, 678, 1024
BstMCI CGRYCG 1 cut(s) 442
BstMWI GCNNNNNNNGC 4 cut(s) 372, 606, 719, 919
BstNI CCWGG 2 cut(s) 269, 924
BstNSI RCATGY 2 cut(s) 370, 600
BstSCI CCNGG 2 cut(s) 267, 922
BstSFI CTRYAG 1 cut(s) 915
BstSLI GKGCMC 1 cut(s) 1075
BstV1I GCAGC 1 cut(s) 700
BstV2I GAAGAC 3 cut(s) 110, 597, 975
BstXI CCANNNNNNTGG 1 cut(s) 1215
BsuRI GGCC 5 cut(s) 67, 99, 404, 520, 1282
BtgI CCRYGG 1 cut(s) 1114
BtsCI GGATG 4 cut(s) 22, 159, 396, 1261
BtsIMutI CAGTG 5 cut(s) 552, 804, 924, 1019, 1213
Cac8I GCNNGC 3 cut(s) 522, 598, 1017
CaiI CAGNNNCTG 2 cut(s) 883, 923
CciI TCATGA 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 824, 1281
CseI GACGC 1 cut(s) 602
Csp6I GTAC 4 cut(s) 223, 811, 961, 1111
CspCI CAANNNNNGTGG 2 cut(s) 208, 243
CviAII CATG 9 cut(s) 8, 274, 367, 380, 462, 569, 597, 770, 902
CviQI GTAC 4 cut(s) 223, 811, 961, 1111
DdeI CTNAG 2 cut(s) 544, 884
DpnI GATC 7 cut(s) 54, 282, 359, 540, 644, 680, 1026
DpnII GATC 7 cut(s) 52, 280, 357, 538, 642, 678, 1024
DraI TTTAAA 1 cut(s) 1188
EaeI YGGCCR 2 cut(s) 402, 518
Eam1104I CTCTTC 1 cut(s) 439
EarI CTCTTC 1 cut(s) 439
Eco130I CCWWGG 2 cut(s) 244, 986
Eco147I AGGCCT 1 cut(s) 99
Eco32I GATATC 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 824
Eco72I CACGTG 1 cut(s) 234
Eco88I CYCGRG 1 cut(s) 1274
EcoO109I RGGNCCY 1 cut(s) 824
EcoRI GAATTC 1 cut(s) 173
EcoRII CCWGG 2 cut(s) 267, 922
EcoRV GATATC 1 cut(s) 305
EcoT14I CCWWGG 2 cut(s) 244, 986
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 244, 986
FaeI CATG 9 cut(s) 11, 277, 370, 383, 465, 572, 600, 773, 905
FaqI GGGAC 1 cut(s) 768
FatI CATG 9 cut(s) 7, 273, 366, 379, 461, 568, 596, 769, 901
Fnu4HI GCNGC 2 cut(s) 714, 1220
FokI GGATG 4 cut(s) 29, 146, 403, 1268
Fsp4HI GCNGC 2 cut(s) 714, 1220
FspBI CTAG 5 cut(s) 41, 122, 635, 828, 1347
GluI GCNGC 2 cut(s) 714, 1220
HaeIII GGCC 5 cut(s) 67, 99, 404, 520, 1282
HapII CCGG 1 cut(s) 517
HgaI GACGC 1 cut(s) 602
Hin1I GRCGYC 1 cut(s) 417
Hin1II CATG 9 cut(s) 11, 277, 370, 383, 465, 572, 600, 773, 905
HincII GTYRAC 1 cut(s) 1105
HindII GTYRAC 1 cut(s) 1105
HinfI GANTC 3 cut(s) 194, 435, 557
HpaII CCGG 1 cut(s) 517
HphI GGTGA 4 cut(s) 182, 1255, 1289, 1307
Hpy166II GTNNAC 7 cut(s) 626, 659, 811, 1073, 1105, 1168, 1315
Hpy188I TCNGA 3 cut(s) 76, 149, 164
Hpy188III TCNNGA 7 cut(s) 8, 56, 122, 646, 727, 1082, 1274
Hpy8I GTNNAC 7 cut(s) 626, 659, 811, 1073, 1105, 1168, 1315
Hpy99I CGWCG 1 cut(s) 422
HpyAV CCTTC 3 cut(s) 79, 107, 1114
HpyCH4III ACNGT 5 cut(s) 227, 475, 808, 919, 1312
HpyCH4IV ACGT 3 cut(s) 233, 399, 417
HpyF10VI GCNNNNNNNGC 4 cut(s) 372, 606, 719, 919
HpyF3I CTNAG 2 cut(s) 544, 884
HpySE526I ACGT 3 cut(s) 233, 399, 417
Hsp92I GRCGYC 1 cut(s) 417
Hsp92II CATG 9 cut(s) 11, 277, 370, 383, 465, 572, 600, 773, 905
KpnI GGTACC 1 cut(s) 1114
Kzo9I GATC 7 cut(s) 52, 280, 357, 538, 642, 678, 1024
LmnI GCTCC 3 cut(s) 372, 657, 1012
Lsp1109I GCAGC 1 cut(s) 700
LweI GCATC 1 cut(s) 13
MaeI CTAG 5 cut(s) 41, 122, 635, 828, 1347
MaeII ACGT 3 cut(s) 233, 399, 417
MaeIII GTNAC 1 cut(s) 475
MalI GATC 7 cut(s) 54, 282, 359, 540, 644, 680, 1026
MboI GATC 7 cut(s) 52, 280, 357, 538, 642, 678, 1024
MboII GAAGA 8 cut(s) 110, 250, 334, 367, 456, 602, 980, 1262
MhlI GDGCHC 3 cut(s) 253, 377, 1075
MluCI AATT 5 cut(s) 90, 173, 337, 795, 1355
MlyI GAGTC 2 cut(s) 429, 566
MmeI TCCRAC 4 cut(s) 52, 172, 345, 765
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 117, 630
MseI TTAA 3 cut(s) 102, 843, 1187
MspCI CTTAAG 1 cut(s) 842
MspI CCGG 1 cut(s) 517
MspR9I CCNGG 2 cut(s) 269, 924
MvaI CCWGG 2 cut(s) 269, 924
MwoI GCNNNNNNNGC 4 cut(s) 372, 606, 719, 919
NdeII GATC 7 cut(s) 52, 280, 357, 538, 642, 678, 1024
NlaIII CATG 9 cut(s) 11, 277, 370, 383, 465, 572, 600, 773, 905
NlaIV GGNNCC 5 cut(s) 566, 653, 825, 912, 1112
NmuCI GTSAC 1 cut(s) 475
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 370, 600
NspV TTCGAA 1 cut(s) 261
PaeI GCATGC 1 cut(s) 600
PagI TCATGA 1 cut(s) 7
PceI AGGCCT 1 cut(s) 99
PdmI GAANNNNTTC 2 cut(s) 117, 630
PfeI GAWTC 1 cut(s) 194
PflFI GACNNNGTC 1 cut(s) 438
PkrI GCNGC 2 cut(s) 715, 1221
PleI GAGTC 2 cut(s) 429, 565
PmaCI CACGTG 1 cut(s) 234
PmlI CACGTG 1 cut(s) 234
PpsI GAGTC 2 cut(s) 429, 565
Ppu21I YACGTR 2 cut(s) 234, 400
PpuMI RGGWCCY 1 cut(s) 824
Psp5II RGGWCCY 1 cut(s) 824
Psp6I CCWGG 2 cut(s) 267, 922
PspCI CACGTG 1 cut(s) 234
PspGI CCWGG 2 cut(s) 267, 922
PspN4I GGNNCC 5 cut(s) 566, 653, 825, 912, 1112
PspPI GGNCC 2 cut(s) 824, 1281
PspPPI RGGWCCY 1 cut(s) 824
PstNI CAGNNNCTG 2 cut(s) 883, 923
PsyI GACNNNGTC 1 cut(s) 438
RsaI GTAC 4 cut(s) 224, 812, 962, 1112
RsaNI GTAC 4 cut(s) 223, 811, 961, 1111
SaqAI TTAA 3 cut(s) 102, 843, 1187
SatI GCNGC 2 cut(s) 714, 1220
Sau3AI GATC 7 cut(s) 52, 280, 357, 538, 642, 678, 1024
Sau96I GGNCC 2 cut(s) 824, 1281
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 2 cut(s) 429, 566
ScrFI CCNGG 2 cut(s) 269, 924
SduI GDGCHC 3 cut(s) 253, 377, 1075
SfaNI GCATC 1 cut(s) 13
SfcI CTRYAG 1 cut(s) 915
SfuI TTCGAA 1 cut(s) 261
SinI GGWCC 1 cut(s) 824
SmlI CTYRAG 4 cut(s) 727, 842, 1046, 1322
SmoI CTYRAG 4 cut(s) 727, 842, 1046, 1322
SphI GCATGC 1 cut(s) 600
Sse9I AATT 5 cut(s) 90, 173, 337, 795, 1355
SseBI AGGCCT 1 cut(s) 99
SsiI CCGC 1 cut(s) 1219
SspMI CTAG 5 cut(s) 41, 122, 635, 828, 1347
StuI AGGCCT 1 cut(s) 99
StyD4I CCNGG 2 cut(s) 267, 922
StyI CCWWGG 2 cut(s) 244, 986
TaaI ACNGT 5 cut(s) 227, 475, 808, 919, 1312
TaiI ACGT 3 cut(s) 236, 402, 420
TaqI TCGA 7 cut(s) 207, 261, 283, 353, 420, 442, 555
TaqII GACCGA 1 cut(s) 428
TasI AATT 5 cut(s) 90, 173, 337, 795, 1355
TatI WGTACW 3 cut(s) 222, 810, 960
TauI GCSGC 1 cut(s) 1222
TfiI GAWTC 1 cut(s) 194
Tru1I TTAA 3 cut(s) 102, 843, 1187
Tru9I TTAA 3 cut(s) 102, 843, 1187
TscAI CASTG 5 cut(s) 552, 811, 924, 1026, 1220
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 1 cut(s) 713
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 3 cut(s) 33, 438, 969
TspGWI ACGGA 1 cut(s) 644
TspRI CASTG 5 cut(s) 552, 811, 924, 1026, 1220
Tth111I GACNNNGTC 1 cut(s) 438
Vha464I CTTAAG 1 cut(s) 842
VneI GTGCAC 1 cut(s) 1071
VpaK11BI GGWCC 1 cut(s) 824
XapI RAATTY 3 cut(s) 90, 173, 795
XbaI TCTAGA 1 cut(s) 121
XceI RCATGY 2 cut(s) 370, 600
XmnI GAANNNNTTC 2 cut(s) 117, 630
XspI CTAG 5 cut(s) 41, 122, 635, 828, 1347
ZraI GACGTC 1 cut(s) 418
ZrmI AGTACT 1 cut(s) 224
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.