Rh5CG379400

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0017107
Physical Location & Seq
Reverse (-)
49496155 .. 49498114
1960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG379400.1

Sequence Viewer

Length: 1572 bp
ATGACTTCTCTTCTATTATCTCCAAACCAACCCAATATCAATCCTACACTACCACCAAACTCTCAAGGCCTTTTGCAAAGACATTACCTGATCAACAAAACCATAAACCTGATATTTCCAAACATACCCTATTCACCAAATCTAAAAACCACTACCAAATGCTCATCATCATCATCATCATCACTCTCAAGCGCCCCAAGCATACATCATGAGTGGGATGAAAATATCCAACCTGAAACCCTAGTCTCCCACGATCAGGAACAAAGGCCGCTATCCGAAATGTGGAAGGAAATGCAAGGCCTTAATAACTGGGAAGGCCTTCTAGACCCAACACTCAATCCCCATCTTCGACAAGAAATCATCCGATATGGTGAATTCGCCCAAGCCTCTTACGATTCCTTTGACTTCGACCCTCACTCCAAGTACTGTGGCACGTGTAAATACCAAGGTGCTCATTTCTTCGAAAACCTGGACATGGCAGATCGAGGCTACCAAATCAGCCGATATCTATATGCAACTTCCAACATCAATCTCCCCAATTTCTTCCAAAAGTCGAAGGTCAGTAGCGTGTGGAGCACCCATGCAAACTGGATGGGCTACGTGGCCGTAGCCACAGACGTCGATGAAATCAAAAGACTTGGTCGAAGAGACATAGTGATTGCATGGAGAGGAACAGTCACATACCTTGAATGGATTTACGACCTCAAAGACATTCTCCGGCCAGCTTATTTTTGCAGTGATCCCTCAGTGAAGATCGAATCTGGGTTCCATGAATTATACACCAAGAAAGAAGATACGTGCAAATACTGCTCATTTTCTGCTCGTGAACAAGTTCTAGCAGAGATCAAGAGGCTCCGTGAACGCTACGAAGGAGAAGAGATTAGTATTACAATCACAGGTCATAGTCTTGGGGCTGCTTTGGCTATTTTGAGTGCCTATGATATTGCAGAAATGGGACTAGATGTTATACGTGATGATGGTAGGGTGGAGACTAAAATTCCAATCACCGTGTACTCATTTTCGGGTCCTAGAGTGGGAAATCTTAAGTTCAAGGAAAGATGTGATGAGCTTGGAGTTAAGGTGCTGAGAGTCATCAATGTGCATGATAAGGTACCTACAGTGCCTGGTATCTTTGCAAATGAGAAGTTTAGGTATCAAAAGTACATAGAAGACACTATTTCATTTCCTTGGAGTTATGCTCATGTTGGAGTGGAGCTTGTACTGGATCACACCCAAAGCCCATTTCTCAAGCCCACTAATGATTTTGGGTGTGCACACAATCTTGAAGCTCATTTGCATTTGGTTGATGGGTACCATGGGAAGGGCAAAAAGTTCTGCTTGGTTACCAAGAGGGATATTGCACTTGTGAACAAGAGCTGTGATTTTTTGAAGAGCCAATATGGCGTGCCGCCACACTGGCGGCAAGATGAGAACAAGGGTATGGTGAGGAATAGGGATGGGAGGTGGGTTCTGCCAGAGAGGCCCAAAGTTGAAGCTCACCCACCTGATATGGTTCACCACCTTGAGCAACTGCTCAATAAATCATCTGGCTCTCAACTTGAAATGCCTTGA

Protein Analysis

523

Amino Acids

59.89

Weight (kDa)

6.5

Isoelectric Point (pI)

41.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 219 - 377 1.1e-37 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017107)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51440
prunus_persica Prupe.4G253800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0052751
rosa_laevigata RLG00000034801
rosa_multiflora Rmu_sc0010265.1_g000002 Rmu_sc0021934.1_g000012
rosa_roxburghii Rroxscaffold_1G00028560
rosa_rugosa Rorug05G0270200
rosa_samantha Rh5AG342200 Rh5BG352900 Rh5CG379400 Rh5DG369200
rosa_wichuraiana Rw5G032260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 621
Acc65I GGTACC 2 cut(s) 1111, 1311
AccB1I GGYRCC 2 cut(s) 1111, 1311
AciI CCGC 3 cut(s) 269, 1409, 1420
AclWI GGATC 2 cut(s) 734, 1233
AcoI YGGCCR 2 cut(s) 603, 719
AcsI RAATTY 2 cut(s) 374, 996
AcvI CACGTG 1 cut(s) 435
AcyI GRCGYC 1 cut(s) 618
AfaI GTAC 6 cut(s) 425, 1013, 1113, 1163, 1221, 1313
AfiI CCNNNNNNNGG 5 cut(s) 256, 282, 475, 1034, 1321
AflII CTTAAG 1 cut(s) 1043
AflIII ACRYGT 1 cut(s) 434
AgsI TTSAA 6 cut(s) 689, 1051, 1286, 1390, 1493, 1562
AjnI CCWGG 2 cut(s) 468, 1123
AjuI GAANNNNNNNTTGG 2 cut(s) 1227, 1259
AluBI AGCT 6 cut(s) 725, 1069, 1216, 1289, 1377, 1496
AluI AGCT 6 cut(s) 725, 1069, 1216, 1289, 1377, 1496
Alw21I GWGCWC 3 cut(s) 454, 578, 1276
Alw26I GTCTC 3 cut(s) 250, 642, 983
Alw44I GTGCAC 1 cut(s) 1272
AlwI GGATC 2 cut(s) 734, 1233
AlwNI CAGNNNCTG 1 cut(s) 1124
AoxI GGCC 7 cut(s) 67, 266, 298, 316, 603, 719, 1481
ApaLI GTGCAC 1 cut(s) 1272
ApeKI GCWGC 1 cut(s) 914
ApoI RAATTY 2 cut(s) 374, 996
Asp700I GAANNNNTTC 2 cut(s) 318, 831
Asp718I GGTACC 2 cut(s) 1111, 1311
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 2 cut(s) 1025, 1482
AsuHPI GGTGA 6 cut(s) 126, 383, 997, 1456, 1490, 1508
AsuII TTCGAA 1 cut(s) 462
AvaII GGWCC 1 cut(s) 1025
BaeGI GKGCMC 1 cut(s) 1276
BanI GGYRCC 2 cut(s) 1111, 1311
BauI CACGAG 1 cut(s) 822
BbrPI CACGTG 1 cut(s) 435
BbsI GAAGAC 1 cut(s) 1176
Bbv12I GWGCWC 3 cut(s) 454, 578, 1276
BbvI GCAGC 1 cut(s) 901
BccI CCATC 5 cut(s) 351, 586, 971, 1301, 1451
BceAI ACGGC 1 cut(s) 590
BciT130I CCWGG 2 cut(s) 470, 1125
BclI TGATCA 1 cut(s) 90
BcoDI GTCTC 3 cut(s) 250, 642, 983
BfaI CTAG 5 cut(s) 242, 323, 836, 959, 1029
BfmI CTRYAG 1 cut(s) 1116
BfoI RGCGCY 1 cut(s) 195
BfrI CTTAAG 1 cut(s) 1043
BglI GCCNNNNNGGC 3 cut(s) 1401, 1417, 1480
BisI GCNGC 4 cut(s) 269, 915, 1409, 1421
BlsI GCNGC 4 cut(s) 270, 916, 1410, 1422
BmcAI AGTACT 1 cut(s) 425
Bme1390I CCNGG 2 cut(s) 470, 1125
Bme18I GGWCC 1 cut(s) 1025
BmgT120I GGNCC 2 cut(s) 1025, 1482
BmiI GGNNCC 5 cut(s) 767, 854, 1026, 1113, 1313
BmrFI CCNGG 2 cut(s) 470, 1125
BmrI ACTGGG 1 cut(s) 319
BmuI ACTGGG 1 cut(s) 319
BpiI GAAGAC 1 cut(s) 1176
BplI GAGNNNNNCTC 2 cut(s) 1183, 1215
Bpu14I TTCGAA 1 cut(s) 462
BpuEI CTTGAG 4 cut(s) 48, 172, 1232, 1544
BsaAI YACGTR 4 cut(s) 435, 601, 798, 971
BsaHI GRCGYC 1 cut(s) 618
BsaJI CCNNGG 3 cut(s) 445, 1187, 1315
BsaXI ACNNNNNCTCC 4 cut(s) 401, 431, 1065, 1095
Bsc4I CCNNNNNNNGG 5 cut(s) 256, 282, 475, 1034, 1321
Bse1I ACTGG 4 cut(s) 314, 593, 1227, 1421
BseBI CCWGG 2 cut(s) 470, 1125
BseDI CCNNGG 3 cut(s) 445, 1187, 1315
BseGI GGATG 4 cut(s) 223, 360, 597, 1462
BseLI CCNNNNNNNGG 5 cut(s) 256, 282, 475, 1034, 1321
BseMII CTCAG 2 cut(s) 759, 1076
BseNI ACTGG 4 cut(s) 314, 593, 1227, 1421
BseSI GKGCMC 1 cut(s) 1276
BseXI GCAGC 1 cut(s) 901
BshFI GGCC 7 cut(s) 69, 268, 300, 318, 605, 721, 1483
BshNI GGYRCC 2 cut(s) 1111, 1311
BsiHKAI GWGCWC 3 cut(s) 454, 578, 1276
BsiSI CCGG 1 cut(s) 718
BslFI GGGAC 1 cut(s) 969
BslI CCNNNNNNNGG 5 cut(s) 256, 282, 475, 1034, 1321
BsmAI GTCTC 3 cut(s) 250, 642, 983
BsmFI GGGAC 1 cut(s) 969
BsnI GGCC 7 cut(s) 69, 268, 300, 318, 605, 721, 1483
Bsp119I TTCGAA 1 cut(s) 462
Bsp1286I GDGCHC 3 cut(s) 454, 578, 1276
Bsp143I GATC 7 cut(s) 90, 253, 481, 739, 753, 843, 1225
Bsp19I CCATGG 1 cut(s) 1315
BspACI CCGC 3 cut(s) 269, 1409, 1420
BspANI GGCC 7 cut(s) 69, 268, 300, 318, 605, 721, 1483
BspCNI CTCAG 2 cut(s) 758, 1077
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 5 cut(s) 767, 854, 1026, 1113, 1313
BspPI GGATC 2 cut(s) 734, 1233
BspQI GCTCTTC 1 cut(s) 1385
BspT104I TTCGAA 1 cut(s) 462
BspT107I GGYRCC 2 cut(s) 1111, 1311
BspTI CTTAAG 1 cut(s) 1043
BsrI ACTGG 4 cut(s) 314, 593, 1227, 1421
BssECI CCNNGG 3 cut(s) 445, 1187, 1315
BssMI GATC 7 cut(s) 90, 253, 481, 739, 753, 843, 1225
BssNI GRCGYC 1 cut(s) 618
BssSI CACGAG 1 cut(s) 822
BssT1I CCWWGG 3 cut(s) 445, 1187, 1315
Bst2BI CACGAG 1 cut(s) 822
Bst2UI CCWGG 2 cut(s) 470, 1125
Bst4CI ACNGT 4 cut(s) 428, 676, 1009, 1120
Bst6I CTCTTC 4 cut(s) 15, 640, 870, 1385
BstACI GRCGYC 1 cut(s) 618
BstAFI CTTAAG 1 cut(s) 1043
BstAPI GCANNNNNTGC 1 cut(s) 807
BstBAI YACGTR 4 cut(s) 435, 601, 798, 971
BstBI TTCGAA 1 cut(s) 462
BstC8I GCNNGC 2 cut(s) 723, 1406
BstDEI CTNAG 2 cut(s) 745, 1085
BstDSI CCRYGG 1 cut(s) 1315
BstEII GGTNACC 1 cut(s) 1342
BstF5I GGATG 4 cut(s) 223, 360, 597, 1462
BstH2I RGCGCY 1 cut(s) 195
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 7 cut(s) 93, 256, 484, 742, 756, 846, 1228
BstMAI GTCTC 3 cut(s) 250, 642, 983
BstMBI GATC 7 cut(s) 90, 253, 481, 739, 753, 843, 1225
BstMWI GCNNNNNNNGC 7 cut(s) 198, 573, 807, 920, 1401, 1417, 1480
BstNI CCWGG 2 cut(s) 470, 1125
BstPI GGTNACC 1 cut(s) 1342
BstSCI CCNGG 2 cut(s) 468, 1123
BstSFI CTRYAG 1 cut(s) 1116
BstSLI GKGCMC 1 cut(s) 1276
BstV1I GCAGC 1 cut(s) 901
BstV2I GAAGAC 1 cut(s) 1176
BsuRI GGCC 7 cut(s) 69, 268, 300, 318, 605, 721, 1483
BtgI CCRYGG 1 cut(s) 1315
BtsCI GGATG 4 cut(s) 223, 360, 597, 1462
BtsI GCAGTG 1 cut(s) 742
BtsIMutI CAGTG 4 cut(s) 742, 753, 1125, 1414
Cac8I GCNNGC 2 cut(s) 723, 1406
CaiI CAGNNNCTG 1 cut(s) 1124
CciI TCATGA 1 cut(s) 208
CfoI GCGC 1 cut(s) 194
Cfr13I GGNCC 2 cut(s) 1025, 1482
Csp6I GTAC 6 cut(s) 424, 1012, 1112, 1162, 1220, 1312
CspCI CAANNNNNGTGG 2 cut(s) 409, 444
CviAII CATG 8 cut(s) 209, 475, 581, 663, 770, 1103, 1202, 1316
CviQI GTAC 6 cut(s) 424, 1012, 1112, 1162, 1220, 1312
DdeI CTNAG 2 cut(s) 745, 1085
DpnI GATC 7 cut(s) 92, 255, 483, 741, 755, 845, 1227
DpnII GATC 7 cut(s) 90, 253, 481, 739, 753, 843, 1225
EaeI YGGCCR 2 cut(s) 603, 719
Eam1104I CTCTTC 4 cut(s) 15, 640, 870, 1385
EarI CTCTTC 4 cut(s) 15, 640, 870, 1385
Eco130I CCWWGG 3 cut(s) 445, 1187, 1315
Eco147I AGGCCT 3 cut(s) 69, 300, 318
Eco32I GATATC 1 cut(s) 506
Eco47I GGWCC 1 cut(s) 1025
Eco72I CACGTG 1 cut(s) 435
Eco91I GGTNACC 1 cut(s) 1342
EcoO109I RGGNCCY 1 cut(s) 1025
EcoO65I GGTNACC 1 cut(s) 1342
EcoRI GAATTC 1 cut(s) 374
EcoRII CCWGG 2 cut(s) 468, 1123
EcoRV GATATC 1 cut(s) 506
EcoT14I CCWWGG 3 cut(s) 445, 1187, 1315
ErhI CCWWGG 3 cut(s) 445, 1187, 1315
FaeI CATG 8 cut(s) 212, 478, 584, 666, 773, 1106, 1205, 1319
FalI AAGNNNNNCTT 2 cut(s) 1322, 1354
FaqI GGGAC 1 cut(s) 969
FatI CATG 8 cut(s) 208, 474, 580, 662, 769, 1102, 1201, 1315
FbaI TGATCA 1 cut(s) 90
Fnu4HI GCNGC 4 cut(s) 269, 915, 1409, 1421
FokI GGATG 4 cut(s) 230, 347, 604, 1469
Fsp4HI GCNGC 4 cut(s) 269, 915, 1409, 1421
FspBI CTAG 5 cut(s) 242, 323, 836, 959, 1029
GlaI GCGC 1 cut(s) 193
GluI GCNGC 4 cut(s) 269, 915, 1409, 1421
HaeII RGCGCY 1 cut(s) 195
HaeIII GGCC 7 cut(s) 69, 268, 300, 318, 605, 721, 1483
HapII CCGG 1 cut(s) 718
HhaI GCGC 1 cut(s) 194
Hin1I GRCGYC 1 cut(s) 618
Hin1II CATG 8 cut(s) 212, 478, 584, 666, 773, 1106, 1205, 1319
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HinfI GANTC 3 cut(s) 395, 758, 1089
HpaII CCGG 1 cut(s) 718
HphI GGTGA 6 cut(s) 126, 383, 997, 1456, 1490, 1508
Hpy166II GTNNAC 6 cut(s) 827, 860, 1012, 1274, 1369, 1516
Hpy188I TCNGA 2 cut(s) 277, 365
Hpy188III TCNNGA 6 cut(s) 209, 257, 323, 824, 847, 1283
Hpy8I GTNNAC 6 cut(s) 827, 860, 1012, 1274, 1369, 1516
Hpy99I CGWCG 1 cut(s) 623
HpyAV CCTTC 6 cut(s) 280, 308, 329, 550, 863, 1315
HpyCH4III ACNGT 4 cut(s) 428, 676, 1009, 1120
HpyCH4IV ACGT 5 cut(s) 434, 600, 618, 797, 970
HpyF10VI GCNNNNNNNGC 7 cut(s) 198, 573, 807, 920, 1401, 1417, 1480
HpyF3I CTNAG 2 cut(s) 745, 1085
HpySE526I ACGT 5 cut(s) 434, 600, 618, 797, 970
Hsp92I GRCGYC 1 cut(s) 618
Hsp92II CATG 8 cut(s) 212, 478, 584, 666, 773, 1106, 1205, 1319
HspAI GCGC 1 cut(s) 192
KpnI GGTACC 2 cut(s) 1115, 1315
Ksp22I TGATCA 1 cut(s) 90
Kzo9I GATC 7 cut(s) 90, 253, 481, 739, 753, 843, 1225
LguI GCTCTTC 1 cut(s) 1385
LmnI GCTCC 3 cut(s) 573, 858, 1213
Lsp1109I GCAGC 1 cut(s) 901
MaeI CTAG 5 cut(s) 242, 323, 836, 959, 1029
MaeII ACGT 5 cut(s) 434, 600, 618, 797, 970
MaeIII GTNAC 2 cut(s) 676, 1342
MalI GATC 7 cut(s) 92, 255, 483, 741, 755, 845, 1227
MboI GATC 7 cut(s) 90, 253, 481, 739, 753, 843, 1225
MboII GAAGA 9 cut(s) 338, 451, 535, 657, 763, 803, 887, 1181, 1402
MhlI GDGCHC 3 cut(s) 454, 578, 1276
MluCI AATT 4 cut(s) 374, 538, 773, 996
MlyI GAGTC 1 cut(s) 1098
MmeI TCCRAC 3 cut(s) 253, 546, 1186
MroXI GAANNNNTTC 2 cut(s) 318, 831
MseI TTAA 3 cut(s) 303, 1044, 1077
MslI CAYNNNNRTG 1 cut(s) 1097
MspCI CTTAAG 1 cut(s) 1043
MspI CCGG 1 cut(s) 718
MspR9I CCNGG 2 cut(s) 470, 1125
MvaI CCWGG 2 cut(s) 470, 1125
MwoI GCNNNNNNNGC 7 cut(s) 198, 573, 807, 920, 1401, 1417, 1480
NcoI CCATGG 1 cut(s) 1315
NdeII GATC 7 cut(s) 90, 253, 481, 739, 753, 843, 1225
NlaIII CATG 8 cut(s) 212, 478, 584, 666, 773, 1106, 1205, 1319
NlaIV GGNNCC 5 cut(s) 767, 854, 1026, 1113, 1313
NmuCI GTSAC 1 cut(s) 676
NspV TTCGAA 1 cut(s) 462
PagI TCATGA 1 cut(s) 208
PceI AGGCCT 3 cut(s) 69, 300, 318
PciSI GCTCTTC 1 cut(s) 1385
PdmI GAANNNNTTC 2 cut(s) 318, 831
PfeI GAWTC 2 cut(s) 395, 758
PflFI GACNNNGTC 1 cut(s) 639
PkrI GCNGC 4 cut(s) 270, 916, 1410, 1422
PleI GAGTC 1 cut(s) 1097
PmaCI CACGTG 1 cut(s) 435
PmlI CACGTG 1 cut(s) 435
PpsI GAGTC 1 cut(s) 1097
Ppu21I YACGTR 4 cut(s) 435, 601, 798, 971
PpuMI RGGWCCY 1 cut(s) 1025
Psp5II RGGWCCY 1 cut(s) 1025
Psp6I CCWGG 2 cut(s) 468, 1123
PspCI CACGTG 1 cut(s) 435
PspEI GGTNACC 1 cut(s) 1342
PspGI CCWGG 2 cut(s) 468, 1123
PspN4I GGNNCC 5 cut(s) 767, 854, 1026, 1113, 1313
PspPI GGNCC 2 cut(s) 1025, 1482
PspPPI RGGWCCY 1 cut(s) 1025
PstNI CAGNNNCTG 1 cut(s) 1124
PsyI GACNNNGTC 1 cut(s) 639
RsaI GTAC 6 cut(s) 425, 1013, 1113, 1163, 1221, 1313
RsaNI GTAC 6 cut(s) 424, 1012, 1112, 1162, 1220, 1312
RseI CAYNNNNRTG 1 cut(s) 1097
SapI GCTCTTC 1 cut(s) 1385
SaqAI TTAA 3 cut(s) 303, 1044, 1077
SatI GCNGC 4 cut(s) 269, 915, 1409, 1421
Sau3AI GATC 7 cut(s) 90, 253, 481, 739, 753, 843, 1225
Sau96I GGNCC 2 cut(s) 1025, 1482
ScaI AGTACT 1 cut(s) 425
SchI GAGTC 1 cut(s) 1098
ScrFI CCNGG 2 cut(s) 470, 1125
SduI GDGCHC 3 cut(s) 454, 578, 1276
SfcI CTRYAG 1 cut(s) 1116
SfuI TTCGAA 1 cut(s) 462
SinI GGWCC 1 cut(s) 1025
SmiMI CAYNNNNRTG 1 cut(s) 1097
SmlI CTYRAG 5 cut(s) 63, 187, 1043, 1247, 1523
SmoI CTYRAG 5 cut(s) 63, 187, 1043, 1247, 1523
Sse9I AATT 4 cut(s) 374, 538, 773, 996
SseBI AGGCCT 3 cut(s) 69, 300, 318
SsiI CCGC 3 cut(s) 269, 1409, 1420
SspMI CTAG 5 cut(s) 242, 323, 836, 959, 1029
StuI AGGCCT 3 cut(s) 69, 300, 318
StyD4I CCNGG 2 cut(s) 468, 1123
StyI CCWWGG 3 cut(s) 445, 1187, 1315
TaaI ACNGT 4 cut(s) 428, 676, 1009, 1120
TaiI ACGT 5 cut(s) 437, 603, 621, 800, 973
TaqI TCGA 8 cut(s) 349, 408, 462, 484, 554, 621, 643, 756
TasI AATT 4 cut(s) 374, 538, 773, 996
TatI WGTACW 4 cut(s) 423, 1011, 1161, 1219
TauI GCSGC 3 cut(s) 271, 1411, 1423
TfiI GAWTC 2 cut(s) 395, 758
Tru1I TTAA 3 cut(s) 303, 1044, 1077
Tru9I TTAA 3 cut(s) 303, 1044, 1077
TscAI CASTG 4 cut(s) 742, 753, 1125, 1421
TseFI GTSAC 1 cut(s) 676
TseI GCWGC 1 cut(s) 914
Tsp45I GTSAC 1 cut(s) 676
TspDTI ATGAA 4 cut(s) 234, 639, 786, 1170
TspGWI ACGGA 1 cut(s) 845
TspRI CASTG 4 cut(s) 742, 753, 1125, 1421
Tth111I GACNNNGTC 1 cut(s) 639
Vha464I CTTAAG 1 cut(s) 1043
VneI GTGCAC 1 cut(s) 1272
VpaK11BI GGWCC 1 cut(s) 1025
XapI RAATTY 2 cut(s) 374, 996
XbaI TCTAGA 1 cut(s) 322
XmnI GAANNNNTTC 2 cut(s) 318, 831
XspI CTAG 5 cut(s) 242, 323, 836, 959, 1029
ZraI GACGTC 1 cut(s) 619
ZrmI AGTACT 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.