Rroxscaffold_1G00030330

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0020714
Physical Location & Seq
Forward (+)
39829902 .. 39833619
3718 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00030330.1

Sequence Viewer

Length: 603 bp
ATGGCAGTTGCTGCAAGGCGACGAGGCGAGAGTAGTAGGACCCGATTGGTTCTCGTGATTTCGACGACGGCGTGGCTTCAAACTTGCCTTGTTAAGCTCGTAGTGGCCTCCTCGTTCGATCCTTGGTGGTGGTTTCGAGTGATTCTCTATGTGGCATTGCAGCTCAAGAGCTTTGGATTTGGCTGGAATTCACACTTTCGAGATGACGATGAAATTGAGAGAGGAGCAGAGATAGCGAGAGAGCTGCGAAGGCTATCCAGAATCACAGTATCAATAATCGTGTTTTCCAAGAATTATGCTTCCTCAAGATGGTGCTTGGATGAGCTTGCCACTATCATGAAACGTAGAAGAAAAGATGGACACGTGGTTTTGCCGTTTTCTATAAGGTGGATCCATCCAATGTTAGACATCGATCGGCGCTTTAAAGAAGCGTTTGCCAAACATGAAGAGCGCTTCAAGGAGGAGATGGGCAAGGTGCACGAGTGGAGAAGATCTCTTAAAGATGCCGCCGATCCGGGTGGGATGGAATTAGGAGATCGGTATGAATCACAGCTGATCCAAAATATTGTTGAGAAGATTGGAAATAAACCGGAACCATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

200

Amino Acids

23.52

Weight (kDa)

9.72

Isoelectric Point (pI)

63.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 46 - 198 2e-23 TIR domain
TIR_2 PF13676 62 - 139 2.4e-07 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020714)

Species Orthologous Gene IDs
rosa_laevigata RLG00000032417
rosa_roxburghii Rroxscaffold_1G00030330 Rroxscaffold_4G00283110 Rroxscaffold_5G00366870 Rroxscaffold_5G00366980
rosa_samantha Rh2AG031100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 507
AclWI GGATC 5 cut(s) 113, 385, 398, 506, 550
AcsI RAATTY 1 cut(s) 187
AcvI CACGTG 1 cut(s) 364
AfeI AGCGCT 1 cut(s) 452
AfiI CCNNNNNNNGG 1 cut(s) 309
AflIII ACRYGT 1 cut(s) 361
AgsI TTSAA 2 cut(s) 80, 457
AluBI AGCT 6 cut(s) 97, 163, 171, 244, 325, 553
AluI AGCT 6 cut(s) 97, 163, 171, 244, 325, 553
Alw21I GWGCWC 1 cut(s) 480
Alw44I GTGCAC 1 cut(s) 476
AlwI GGATC 5 cut(s) 113, 385, 398, 506, 550
AlwNI CAGNNNCTG 1 cut(s) 11
Aor51HI AGCGCT 1 cut(s) 452
AoxI GGCC 1 cut(s) 105
ApaLI GTGCAC 1 cut(s) 476
ApeKI GCWGC 3 cut(s) 11, 160, 244
ApoI RAATTY 1 cut(s) 187
AspLEI GCGC 2 cut(s) 420, 453
AspS9I GGNCC 1 cut(s) 39
AsuC2I CCSGG 1 cut(s) 516
AvaII GGWCC 1 cut(s) 39
BaeGI GKGCMC 1 cut(s) 480
BamHI GGATCC 1 cut(s) 390
BauI CACGAG 2 cut(s) 53, 479
BbrPI CACGTG 1 cut(s) 364
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 2 cut(s) 172, 231
BccI CCATC 5 cut(s) 303, 350, 402, 460, 517
BceAI ACGGC 2 cut(s) 84, 358
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BcnI CCSGG 1 cut(s) 516
BfoI RGCGCY 2 cut(s) 421, 454
BglII AGATCT 1 cut(s) 491
BisI GCNGC 4 cut(s) 12, 161, 245, 507
BlsI GCNGC 4 cut(s) 13, 162, 246, 508
Bme1390I CCNGG 1 cut(s) 516
Bme18I GGWCC 1 cut(s) 39
BmgT120I GGNCC 1 cut(s) 39
BmiI GGNNCC 3 cut(s) 41, 392, 594
BmrFI CCNGG 1 cut(s) 516
BmsI GCATC 1 cut(s) 493
BplI GAGNNNNNCTC 4 cut(s) 129, 161, 478, 510
BpuEI CTTGAG 2 cut(s) 149, 289
BpuMI CCSGG 1 cut(s) 516
Bsa29I ATCGAT 1 cut(s) 411
BsaAI YACGTR 1 cut(s) 364
BsaJI CCNNGG 1 cut(s) 122
BsaWI WCCGGW 1 cut(s) 589
Bsc4I CCNNNNNNNGG 1 cut(s) 309
Bse3DI GCAATG 1 cut(s) 155
BseCI ATCGAT 1 cut(s) 411
BseDI CCNNGG 1 cut(s) 122
BseGI GGATG 3 cut(s) 325, 394, 528
BseLI CCNNNNNNNGG 1 cut(s) 309
BseMI GCAATG 1 cut(s) 155
BseRI GAGGAG 3 cut(s) 100, 237, 476
BseSI GKGCMC 1 cut(s) 480
BseXI GCAGC 2 cut(s) 172, 231
Bsh1285I CGRYCG 1 cut(s) 415
BshFI GGCC 1 cut(s) 107
BshVI ATCGAT 1 cut(s) 411
BsiEI CGRYCG 1 cut(s) 415
BsiHKAI GWGCWC 1 cut(s) 480
BsiSI CCGG 2 cut(s) 515, 590
BslI CCNNNNNNNGG 1 cut(s) 309
BsnI GGCC 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 480
Bsp143I GATC 7 cut(s) 118, 390, 412, 491, 511, 535, 555
BspACI CCGC 1 cut(s) 507
BspANI GGCC 1 cut(s) 107
BspDI ATCGAT 1 cut(s) 411
BspHI TCATGA 1 cut(s) 336
BspLI GGNNCC 3 cut(s) 41, 392, 594
BspPI GGATC 5 cut(s) 113, 385, 398, 506, 550
BspQI GCTCTTC 1 cut(s) 441
BsrDI GCAATG 1 cut(s) 155
BssECI CCNNGG 1 cut(s) 122
BssMI GATC 7 cut(s) 118, 390, 412, 491, 511, 535, 555
BssSI CACGAG 2 cut(s) 53, 479
BssT1I CCWWGG 1 cut(s) 122
Bst2BI CACGAG 2 cut(s) 53, 479
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 1 cut(s) 441
BstAPI GCANNNNNTGC 1 cut(s) 11
BstBAI YACGTR 1 cut(s) 364
BstC8I GCNNGC 1 cut(s) 327
BstF5I GGATG 3 cut(s) 325, 394, 528
BstH2I RGCGCY 2 cut(s) 421, 454
BstHHI GCGC 2 cut(s) 420, 453
BstKTI GATC 7 cut(s) 121, 393, 415, 494, 514, 538, 558
BstMBI GATC 7 cut(s) 118, 390, 412, 491, 511, 535, 555
BstMCI CGRYCG 1 cut(s) 415
BstMWI GCNNNNNNNGC 3 cut(s) 11, 233, 250
BstSCI CCNGG 1 cut(s) 514
BstSLI GKGCMC 1 cut(s) 480
BstV1I GCAGC 2 cut(s) 172, 231
BstX2I RGATCY 2 cut(s) 390, 491
BstYI RGATCY 2 cut(s) 390, 491
Bsu15I ATCGAT 1 cut(s) 411
BsuRI GGCC 1 cut(s) 107
BsuTUI ATCGAT 1 cut(s) 411
BtsCI GGATG 3 cut(s) 325, 394, 528
Cac8I GCNNGC 1 cut(s) 327
CaiI CAGNNNCTG 1 cut(s) 11
CciI TCATGA 1 cut(s) 336
CfoI GCGC 2 cut(s) 420, 453
Cfr13I GGNCC 1 cut(s) 39
ClaI ATCGAT 1 cut(s) 411
CviAII CATG 2 cut(s) 337, 443
DpnI GATC 7 cut(s) 120, 392, 414, 493, 513, 537, 557
DpnII GATC 7 cut(s) 118, 390, 412, 491, 511, 535, 555
DraI TTTAAA 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 441
EarI CTCTTC 1 cut(s) 441
Eco130I CCWWGG 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 39
Eco47III AGCGCT 1 cut(s) 452
Eco72I CACGTG 1 cut(s) 364
EcoO109I RGGNCCY 1 cut(s) 39
EcoRI GAATTC 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 122
ErhI CCWWGG 1 cut(s) 122
FaeI CATG 2 cut(s) 340, 446
FaiI YATR 7 cut(s) 150, 297, 338, 383, 444, 543, 601
FatI CATG 2 cut(s) 336, 442
Fnu4HI GCNGC 4 cut(s) 12, 161, 245, 507
FokI GGATG 3 cut(s) 332, 381, 535
Fsp4HI GCNGC 4 cut(s) 12, 161, 245, 507
GlaI GCGC 2 cut(s) 419, 452
GluI GCNGC 4 cut(s) 12, 161, 245, 507
HaeII RGCGCY 2 cut(s) 421, 454
HaeIII GGCC 1 cut(s) 107
HapII CCGG 2 cut(s) 515, 590
HhaI GCGC 2 cut(s) 420, 453
Hin1II CATG 2 cut(s) 340, 446
Hin6I GCGC 2 cut(s) 418, 451
HinP1I GCGC 2 cut(s) 418, 451
HinfI GANTC 3 cut(s) 142, 261, 545
HpaII CCGG 2 cut(s) 515, 590
Hpy166II GTNNAC 1 cut(s) 478
Hpy188III TCNNGA 6 cut(s) 55, 166, 200, 258, 306, 337
Hpy8I GTNNAC 1 cut(s) 478
Hpy99I CGWCG 3 cut(s) 24, 67, 70
HpyAV CCTTC 1 cut(s) 243
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4IV ACGT 2 cut(s) 343, 363
HpyCH4V TGCA 3 cut(s) 14, 160, 478
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 233, 250
HpySE526I ACGT 2 cut(s) 343, 363
Hsp92II CATG 2 cut(s) 340, 446
HspAI GCGC 2 cut(s) 418, 451
Kzo9I GATC 7 cut(s) 118, 390, 412, 491, 511, 535, 555
LguI GCTCTTC 1 cut(s) 441
LmnI GCTCC 1 cut(s) 224
LpnPI CCDG 3 cut(s) 169, 271, 528
Lsp1109I GCAGC 2 cut(s) 172, 231
LweI GCATC 1 cut(s) 493
MaeII ACGT 2 cut(s) 343, 363
MalI GATC 7 cut(s) 120, 392, 414, 493, 513, 537, 557
MboI GATC 7 cut(s) 118, 390, 412, 491, 511, 535, 555
MboII GAAGA 4 cut(s) 360, 458, 501, 586
MflI RGATCY 2 cut(s) 390, 491
MhlI GDGCHC 1 cut(s) 480
MluCI AATT 4 cut(s) 187, 213, 292, 527
MnlI CCTC 6 cut(s) 17, 118, 121, 215, 313, 454
MseI TTAA 3 cut(s) 93, 423, 498
MslI CAYNNNNRTG 1 cut(s) 335
MspA1I CMGCKG 1 cut(s) 553
MspI CCGG 2 cut(s) 515, 590
MspR9I CCNGG 1 cut(s) 516
MwoI GCNNNNNNNGC 3 cut(s) 11, 233, 250
NciI CCSGG 1 cut(s) 516
NdeII GATC 7 cut(s) 118, 390, 412, 491, 511, 535, 555
NlaIII CATG 2 cut(s) 340, 446
NlaIV GGNNCC 3 cut(s) 41, 392, 594
PagI TCATGA 1 cut(s) 336
PciSI GCTCTTC 1 cut(s) 441
PcsI WCGNNNNNNNCGW 1 cut(s) 68
PfeI GAWTC 3 cut(s) 142, 261, 545
PkrI GCNGC 4 cut(s) 13, 162, 246, 508
Ple19I CGATCG 1 cut(s) 415
PmaCI CACGTG 1 cut(s) 364
PmlI CACGTG 1 cut(s) 364
Ppu21I YACGTR 1 cut(s) 364
PpuMI RGGWCCY 1 cut(s) 39
Psp5II RGGWCCY 1 cut(s) 39
PspCI CACGTG 1 cut(s) 364
PspN4I GGNNCC 3 cut(s) 41, 392, 594
PspPI GGNCC 1 cut(s) 39
PspPPI RGGWCCY 1 cut(s) 39
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 2 cut(s) 390, 491
PvuI CGATCG 1 cut(s) 415
PvuII CAGCTG 1 cut(s) 553
RseI CAYNNNNRTG 1 cut(s) 335
SapI GCTCTTC 1 cut(s) 441
SaqAI TTAA 3 cut(s) 93, 423, 498
SatI GCNGC 4 cut(s) 12, 161, 245, 507
Sau3AI GATC 7 cut(s) 118, 390, 412, 491, 511, 535, 555
Sau96I GGNCC 1 cut(s) 39
ScrFI CCNGG 1 cut(s) 516
SduI GDGCHC 1 cut(s) 480
SfaNI GCATC 1 cut(s) 493
SinI GGWCC 1 cut(s) 39
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 2 cut(s) 164, 304
SmoI CTYRAG 2 cut(s) 164, 304
Sse9I AATT 4 cut(s) 187, 213, 292, 527
SsiI CCGC 1 cut(s) 507
SspI AATATT 1 cut(s) 565
StyD4I CCNGG 1 cut(s) 514
StyI CCWWGG 1 cut(s) 122
TaaI ACNGT 1 cut(s) 268
TaiI ACGT 2 cut(s) 346, 366
TaqI TCGA 5 cut(s) 62, 117, 136, 199, 411
TasI AATT 4 cut(s) 187, 213, 292, 527
TauI GCSGC 1 cut(s) 509
TfiI GAWTC 3 cut(s) 142, 261, 545
Tru1I TTAA 3 cut(s) 93, 423, 498
Tru9I TTAA 3 cut(s) 93, 423, 498
TseI GCWGC 3 cut(s) 11, 160, 244
TspDTI ATGAA 4 cut(s) 225, 353, 459, 558
VneI GTGCAC 1 cut(s) 476
VpaK11BI GGWCC 1 cut(s) 39
XapI RAATTY 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.