Rroxscaffold_1G00035000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0023540
Physical Location & Seq
Reverse (-)
50859479 .. 50867376
7898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00035000.1

Sequence Viewer

Length: 579 bp
ATGAATTATAATTGTGCAAAGGGCGATGTGCGAGCTACGCTCGACGTAGGAAACCGAGGCGAGCTTGCTTCGGTGCCGGATTCCGTCGTGGGTCGGCCGGAGAAGGAAGAAATCGAGAGGAGGAAGCATCGGACCGAAGAGGAGAAAAGATCGGGGAAGAAGAGAGAAAAGCTGGGTAAGTTTCCAAAAATGGCAACTTACCACGGGTTCGGGTGCTCTGTGTTGCTCCGGACCAAGGACCAAAATATTGAGTTTCGAGGAAATATCGATGGTCCTGAAAATGGAAATTTCAACGGAAATATCGAGTATATATCGATTTCGGAGTGCTTTGGGACAAATGGATTGCGGCGCGGGCGTGACGAGAGTTCGTCGGTCAAGTCCAAGTCGGGTGCTGCGATGGGTTGTCGGGGTGGTCGGATTCGGATGATGAGGAGGAGTCACAAAAGAAGAAATGGTTTTGGTGATCGGCCACGACGCCAACGAGGATTATCGGTGCGCTCGAAACGCATGCACGGTGATCATCGGCCAGGTAATTCCAAAGAGAAAGAGAGACGAGAGATAGAGAGCCTAGATGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

21.78

Weight (kDa)

10.3

Isoelectric Point (pI)

76.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023540)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003767.1_g000004
rosa_roxburghii Rroxscaffold_1G00035000 Rroxscaffold_2G00084180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 9
AccB1I GGYRCC 1 cut(s) 73
AccII CGCG 1 cut(s) 351
AccIII TCCGGA 1 cut(s) 228
AciI CCGC 2 cut(s) 346, 351
AcoI YGGCCR 3 cut(s) 95, 467, 524
AcsI RAATTY 1 cut(s) 286
AcyI GRCGYC 1 cut(s) 475
AfiI CCNNNNNNNGG 2 cut(s) 235, 281
AgsI TTSAA 1 cut(s) 292
AjnI CCWGG 1 cut(s) 526
AluBI AGCT 3 cut(s) 35, 64, 172
AluI AGCT 3 cut(s) 35, 64, 172
Alw21I GWGCWC 1 cut(s) 218
Alw26I GTCTC 1 cut(s) 544
Aor13HI TCCGGA 1 cut(s) 228
AoxI GGCC 3 cut(s) 95, 467, 524
ApeKI GCWGC 1 cut(s) 392
ApoI RAATTY 1 cut(s) 286
AspLEI GCGC 2 cut(s) 351, 498
AspS9I GGNCC 4 cut(s) 132, 231, 238, 272
AsuHPI GGTGA 2 cut(s) 473, 527
AvaII GGWCC 4 cut(s) 132, 231, 238, 272
BanI GGYRCC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 218
BbvI GCAGC 1 cut(s) 379
BccI CCATC 3 cut(s) 263, 391, 566
BcgI CGANNNNNNTGC 2 cut(s) 490, 524
BciT130I CCWGG 1 cut(s) 528
BclI TGATCA 1 cut(s) 517
BcoDI GTCTC 1 cut(s) 544
BfaI CTAG 1 cut(s) 569
BglI GCCNNNNNGGC 1 cut(s) 573
BisI GCNGC 2 cut(s) 347, 393
BlsI GCNGC 2 cut(s) 348, 394
Bme1390I CCNGG 1 cut(s) 528
Bme18I GGWCC 4 cut(s) 132, 231, 238, 272
BmgT120I GGNCC 4 cut(s) 132, 231, 238, 272
BmiI GGNNCC 1 cut(s) 75
BmrFI CCNGG 1 cut(s) 528
BmsI GCATC 1 cut(s) 136
BplI GAGNNNNNCTC 2 cut(s) 24, 56
Bsa29I ATCGAT 2 cut(s) 267, 314
BsaHI GRCGYC 1 cut(s) 475
BsaJI CCNNGG 3 cut(s) 55, 202, 234
BsaWI WCCGGW 1 cut(s) 228
Bsc4I CCNNNNNNNGG 2 cut(s) 235, 281
BseAI TCCGGA 1 cut(s) 228
BseBI CCWGG 1 cut(s) 528
BseCI ATCGAT 2 cut(s) 267, 314
BseDI CCNNGG 3 cut(s) 55, 202, 234
BseGI GGATG 1 cut(s) 429
BseLI CCNNNNNNNGG 2 cut(s) 235, 281
BseRI GAGGAG 4 cut(s) 133, 155, 445, 448
BseX3I CGGCCG 1 cut(s) 95
BseXI GCAGC 1 cut(s) 379
BseYI CCCAGC 1 cut(s) 172
Bsh1236I CGCG 1 cut(s) 351
Bsh1285I CGRYCG 1 cut(s) 98
BshFI GGCC 3 cut(s) 97, 469, 526
BshNI GGYRCC 1 cut(s) 73
BshVI ATCGAT 2 cut(s) 267, 314
BsiEI CGRYCG 1 cut(s) 98
BsiHKAI GWGCWC 1 cut(s) 218
BsiSI CCGG 3 cut(s) 77, 98, 229
BslFI GGGAC 1 cut(s) 346
BslI CCNNNNNNNGG 2 cut(s) 235, 281
BsmAI GTCTC 1 cut(s) 544
BsmBI CGTCTC 1 cut(s) 544
BsmFI GGGAC 1 cut(s) 346
BsnI GGCC 3 cut(s) 97, 469, 526
Bsp1286I GDGCHC 1 cut(s) 218
Bsp13I TCCGGA 1 cut(s) 228
Bsp143I GATC 3 cut(s) 149, 463, 517
BspACI CCGC 2 cut(s) 346, 351
BspANI GGCC 3 cut(s) 97, 469, 526
BspDI ATCGAT 2 cut(s) 267, 314
BspEI TCCGGA 1 cut(s) 228
BspFNI CGCG 1 cut(s) 351
BspLI GGNNCC 1 cut(s) 75
BspT107I GGYRCC 1 cut(s) 73
BssECI CCNNGG 3 cut(s) 55, 202, 234
BssMI GATC 3 cut(s) 149, 463, 517
BssNI GRCGYC 1 cut(s) 475
BssT1I CCWWGG 1 cut(s) 234
Bst2UI CCWGG 1 cut(s) 528
Bst4CI ACNGT 1 cut(s) 515
Bst6I CTCTTC 2 cut(s) 132, 155
BstACI GRCGYC 1 cut(s) 475
BstC8I GCNNGC 5 cut(s) 33, 62, 66, 353, 509
BstDSI CCRYGG 1 cut(s) 202
BstF5I GGATG 1 cut(s) 429
BstFNI CGCG 1 cut(s) 351
BstHHI GCGC 2 cut(s) 351, 498
BstKTI GATC 3 cut(s) 152, 466, 520
BstMAI GTCTC 1 cut(s) 544
BstMBI GATC 3 cut(s) 149, 463, 517
BstMCI CGRYCG 1 cut(s) 98
BstMWI GCNNNNNNNGC 4 cut(s) 37, 352, 504, 573
BstNI CCWGG 1 cut(s) 528
BstNSI RCATGY 1 cut(s) 511
BstSCI CCNGG 1 cut(s) 526
BstUI CGCG 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 379
BstZI CGGCCG 1 cut(s) 95
Bsu15I ATCGAT 2 cut(s) 267, 314
BsuRI GGCC 3 cut(s) 97, 469, 526
BsuTUI ATCGAT 2 cut(s) 267, 314
BtgI CCRYGG 1 cut(s) 202
BtgZI GCGATG 2 cut(s) 39, 410
BtsCI GGATG 1 cut(s) 429
Cac8I GCNNGC 5 cut(s) 33, 62, 66, 353, 509
CfoI GCGC 2 cut(s) 351, 498
Cfr13I GGNCC 4 cut(s) 132, 231, 238, 272
ClaI ATCGAT 2 cut(s) 267, 314
CpoI CGGWCCG 1 cut(s) 132
CseI GACGC 1 cut(s) 483
CspI CGGWCCG 1 cut(s) 132
CviAII CATG 1 cut(s) 508
CviJI RGCY 8 cut(s) 35, 64, 97, 172, 469, 526, 567, 576
CviKI_1 RGCY 8 cut(s) 35, 64, 97, 172, 469, 526, 567, 576
DpnI GATC 3 cut(s) 151, 465, 519
DpnII GATC 3 cut(s) 149, 463, 517
EaeI YGGCCR 3 cut(s) 95, 467, 524
EagI CGGCCG 1 cut(s) 95
Eam1104I CTCTTC 2 cut(s) 132, 155
EarI CTCTTC 2 cut(s) 132, 155
EclXI CGGCCG 1 cut(s) 95
Eco130I CCWWGG 1 cut(s) 234
Eco47I GGWCC 4 cut(s) 132, 231, 238, 272
Eco52I CGGCCG 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 526
EcoT14I CCWWGG 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 234
Esp3I CGTCTC 1 cut(s) 544
FaeI CATG 1 cut(s) 511
FaiI YATR 4 cut(s) 9, 309, 311, 509
FaqI GGGAC 1 cut(s) 346
FatI CATG 1 cut(s) 507
FauI CCCGC 1 cut(s) 344
FbaI TGATCA 1 cut(s) 517
Fnu4HI GCNGC 2 cut(s) 347, 393
FokI GGATG 1 cut(s) 436
Fsp4HI GCNGC 2 cut(s) 347, 393
FspBI CTAG 1 cut(s) 569
GlaI GCGC 2 cut(s) 350, 497
GluI GCNGC 2 cut(s) 347, 393
GsaI CCCAGC 1 cut(s) 176
HaeIII GGCC 3 cut(s) 97, 469, 526
HapII CCGG 3 cut(s) 77, 98, 229
HgaI GACGC 1 cut(s) 483
HhaI GCGC 2 cut(s) 351, 498
Hin1I GRCGYC 1 cut(s) 475
Hin1II CATG 1 cut(s) 511
Hin6I GCGC 2 cut(s) 349, 496
HinP1I GCGC 2 cut(s) 349, 496
HinfI GANTC 3 cut(s) 80, 418, 436
HpaII CCGG 3 cut(s) 77, 98, 229
HphI GGTGA 2 cut(s) 473, 527
Hpy188I TCNGA 4 cut(s) 132, 322, 417, 423
Hpy188III TCNNGA 3 cut(s) 115, 229, 275
Hpy99I CGWCG 4 cut(s) 47, 89, 373, 477
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 1 cut(s) 515
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 2 cut(s) 17, 511
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 352, 504, 573
HpySE526I ACGT 1 cut(s) 45
Hsp92I GRCGYC 1 cut(s) 475
Hsp92II CATG 1 cut(s) 511
HspAI GCGC 2 cut(s) 349, 496
Kpn2I TCCGGA 1 cut(s) 228
Ksp22I TGATCA 1 cut(s) 517
Kzo9I GATC 3 cut(s) 149, 463, 517
LmnI GCTCC 1 cut(s) 231
LpnPI CCDG 7 cut(s) 90, 111, 158, 242, 288, 513, 540
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 1 cut(s) 136
MaeI CTAG 1 cut(s) 569
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 2 cut(s) 356, 437
MalI GATC 3 cut(s) 151, 465, 519
MboI GATC 3 cut(s) 149, 463, 517
MboII GAAGA 5 cut(s) 119, 149, 169, 172, 459
MhlI GDGCHC 1 cut(s) 218
MluCI AATT 4 cut(s) 4, 10, 286, 532
MlyI GAGTC 1 cut(s) 445
MmeI TCCRAC 1 cut(s) 395
MnlI CCTC 8 cut(s) 50, 111, 114, 133, 251, 423, 426, 476
MroI TCCGGA 1 cut(s) 228
MspI CCGG 3 cut(s) 77, 98, 229
MspR9I CCNGG 1 cut(s) 528
MvaI CCWGG 1 cut(s) 528
MvnI CGCG 1 cut(s) 351
MwoI GCNNNNNNNGC 4 cut(s) 37, 352, 504, 573
NdeII GATC 3 cut(s) 149, 463, 517
NlaIII CATG 1 cut(s) 511
NlaIV GGNNCC 1 cut(s) 75
NmuCI GTSAC 2 cut(s) 356, 437
NspI RCATGY 1 cut(s) 511
PaeI GCATGC 1 cut(s) 511
PcsI WCGNNNNNNNCGW 4 cut(s) 300, 392, 478, 497
PfeI GAWTC 2 cut(s) 80, 418
PkrI GCNGC 2 cut(s) 348, 394
PleI GAGTC 1 cut(s) 444
PpsI GAGTC 1 cut(s) 444
PsiI TTATAA 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 526
PspFI CCCAGC 1 cut(s) 172
PspGI CCWGG 1 cut(s) 526
PspN4I GGNNCC 1 cut(s) 75
PspPI GGNCC 4 cut(s) 132, 231, 238, 272
Rsr2I CGGWCCG 1 cut(s) 132
RsrII CGGWCCG 1 cut(s) 132
SatI GCNGC 2 cut(s) 347, 393
Sau3AI GATC 3 cut(s) 149, 463, 517
Sau96I GGNCC 4 cut(s) 132, 231, 238, 272
SchI GAGTC 1 cut(s) 445
ScrFI CCNGG 1 cut(s) 528
SduI GDGCHC 1 cut(s) 218
SetI ASST 5 cut(s) 37, 48, 66, 174, 532
SfaNI GCATC 1 cut(s) 136
SinI GGWCC 4 cut(s) 132, 231, 238, 272
SphI GCATGC 1 cut(s) 511
Sse9I AATT 4 cut(s) 4, 10, 286, 532
SsiI CCGC 2 cut(s) 346, 351
SspI AATATT 1 cut(s) 247
SspMI CTAG 1 cut(s) 569
StyD4I CCNGG 1 cut(s) 526
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 1 cut(s) 515
TaiI ACGT 1 cut(s) 48
TaqI TCGA 7 cut(s) 42, 114, 256, 267, 303, 314, 500
TaqII GACCGA 2 cut(s) 149, 361
TasI AATT 4 cut(s) 4, 10, 286, 532
TauI GCSGC 1 cut(s) 349
TfiI GAWTC 2 cut(s) 80, 418
TseFI GTSAC 2 cut(s) 356, 437
TseI GCWGC 1 cut(s) 392
Tsp45I GTSAC 2 cut(s) 356, 437
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 73, 309
VpaK11BI GGWCC 4 cut(s) 132, 231, 238, 272
XapI RAATTY 1 cut(s) 286
XceI RCATGY 1 cut(s) 511
XspI CTAG 1 cut(s) 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.