Rroxscaffold_1G00035650

AP-4 complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0020736
Physical Location & Seq
Reverse (-)
51799752 .. 51806386
6635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00035650.1

Sequence Viewer

Length: 558 bp
ATGGAGTCAGATGTATCCAGAATAATGGAGTCAGATATCTACCTCGGGAGACCCAAATCTCGTCCTCAAGCTCATTGGCCACCTCACACTGTCTCCCTCTTCCTCGACGACGATCACAACATCATCATCCGCATCGTCAACACCATCCATTTACACTGCCCTCAATGCCATCTGCAAGCTTATCAACAACGAGACCGTGCCCTCCATTTTTCCCTAGGTCGTGGAGCTCCTAGGGCACCGGAAGGAGGTTGTAAGGAAGAAGGTGATCATGGCTCTTCATCGGTTTTATCGAAATCGCCGTCGTTGGTTTCTTATCCGGTGTCGAATTTTCTAAAGCGACTGCGTGATAACGATCCCGGAGTCATATCGAGCCATGTTGTGCGGCTTGGCTACGGGAAGGTTTGGGAGGAGAAGAAGGACGAGAAAGGTTTGGAGGAGGAAGATGTGGAAGACGAGGAGGGCTCGGAGGGATCCGAGGAGGAGTTGGAAAATGAAGAAGATTCGGATCGGGGGACTTGGAAGAGGAGTCGGAAGAGGAATCGGATGAAGATTCGATGA

Protein Analysis

185

Amino Acids

21.18

Weight (kDa)

5.74

Isoelectric Point (pI)

60.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020736)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0002882.1_g000005 Rmu_sc0003624.1_g000027 Rmu_sc0008929.1_g000001
rosa_roxburghii Rroxscaffold_1G00035650
rosa_samantha Rh3AG116100 Rh4CG249300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 235
AciI CCGC 2 cut(s) 130, 382
AclWI GGATC 4 cut(s) 347, 465, 478, 513
AcoI YGGCCR 1 cut(s) 77
AcsI RAATTY 1 cut(s) 325
AfiI CCNNNNNNNGG 1 cut(s) 245
AluBI AGCT 3 cut(s) 71, 179, 227
AluI AGCT 3 cut(s) 71, 179, 227
Alw21I GWGCWC 1 cut(s) 229
Alw26I GTCTC 3 cut(s) 43, 97, 186
AlwI GGATC 4 cut(s) 347, 465, 478, 513
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 1 cut(s) 77
ApoI RAATTY 1 cut(s) 325
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
AspA2I CCTAGG 2 cut(s) 214, 230
AsuC2I CCSGG 1 cut(s) 357
AsuHPI GGTGA 1 cut(s) 275
AvaI CYCGRG 1 cut(s) 44
AvrII CCTAGG 2 cut(s) 214, 230
BaeGI GKGCMC 2 cut(s) 202, 238
BalI TGGCCA 1 cut(s) 79
BamHI GGATCC 1 cut(s) 470
BanI GGYRCC 1 cut(s) 235
BanII GRGCYC 2 cut(s) 229, 464
BbsI GAAGAC 1 cut(s) 456
Bbv12I GWGCWC 1 cut(s) 229
BccI CCATC 2 cut(s) 152, 177
BceAI ACGGC 1 cut(s) 283
BciVI GTATCC 1 cut(s) 25
BclI TGATCA 1 cut(s) 265
BcnI CCSGG 1 cut(s) 357
BcoDI GTCTC 3 cut(s) 43, 97, 186
BfaI CTAG 2 cut(s) 215, 231
BfuI GTATCC 1 cut(s) 25
BisI GCNGC 1 cut(s) 383
BlnI CCTAGG 2 cut(s) 214, 230
BlsI GCNGC 1 cut(s) 384
Bme1390I CCNGG 1 cut(s) 357
BmeT110I CYCGRG 1 cut(s) 44
BmiI GGNNCC 2 cut(s) 237, 472
BmrFI CCNGG 1 cut(s) 357
BmsI GCATC 1 cut(s) 141
BpiI GAAGAC 1 cut(s) 456
BplI GAGNNNNNCTC 2 cut(s) 446, 478
BpuEI CTTGAG 1 cut(s) 51
BpuMI CCSGG 1 cut(s) 357
BsaBI GATNNNNATC 2 cut(s) 351, 504
BsaI GGTCTC 2 cut(s) 43, 186
BsaJI CCNNGG 4 cut(s) 43, 214, 230, 474
BsaWI WCCGGW 2 cut(s) 238, 316
BsaXI ACNNNNNCTCC 2 cut(s) 77, 107
Bsc4I CCNNNNNNNGG 1 cut(s) 245
Bse8I GATNNNNATC 2 cut(s) 351, 504
BseDI CCNNGG 4 cut(s) 43, 214, 230, 474
BseGI GGATG 3 cut(s) 126, 144, 549
BseJI GATNNNNATC 2 cut(s) 351, 504
BseLI CCNNNNNNNGG 1 cut(s) 245
BseRI GAGGAG 6 cut(s) 422, 449, 470, 491, 494, 538
BseSI GKGCMC 2 cut(s) 202, 238
BshFI GGCC 1 cut(s) 79
BshNI GGYRCC 1 cut(s) 235
BsiHKAI GWGCWC 1 cut(s) 229
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 3 cut(s) 239, 317, 357
BslFI GGGAC 1 cut(s) 526
BslI CCNNNNNNNGG 1 cut(s) 245
BsmAI GTCTC 3 cut(s) 43, 97, 186
BsmFI GGGAC 1 cut(s) 526
BsnI GGCC 1 cut(s) 79
Bso31I GGTCTC 2 cut(s) 43, 186
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 4 cut(s) 202, 229, 238, 464
Bsp143I GATC 5 cut(s) 112, 265, 352, 470, 505
BspACI CCGC 2 cut(s) 130, 382
BspANI GGCC 1 cut(s) 79
BspLI GGNNCC 2 cut(s) 237, 472
BspPI GGATC 4 cut(s) 347, 465, 478, 513
BspQI GCTCTTC 1 cut(s) 280
BspT107I GGYRCC 1 cut(s) 235
BspTNI GGTCTC 2 cut(s) 43, 186
BssECI CCNNGG 4 cut(s) 43, 214, 230, 474
BssMI GATC 5 cut(s) 112, 265, 352, 470, 505
BssT1I CCWWGG 2 cut(s) 214, 230
Bst4CI ACNGT 2 cut(s) 91, 197
Bst6I CTCTTC 4 cut(s) 104, 280, 515, 527
BstC8I GCNNGC 1 cut(s) 177
BstF5I GGATG 3 cut(s) 126, 144, 549
BstKTI GATC 5 cut(s) 115, 268, 355, 473, 508
BstMAI GTCTC 3 cut(s) 43, 97, 186
BstMBI GATC 5 cut(s) 112, 265, 352, 470, 505
BstMWI GCNNNNNNNGC 2 cut(s) 165, 233
BstSCI CCNGG 1 cut(s) 355
BstSLI GKGCMC 2 cut(s) 202, 238
BstV2I GAAGAC 1 cut(s) 456
BstX2I RGATCY 1 cut(s) 470
BstXI CCANNNNNNTGG 1 cut(s) 25
BstYI RGATCY 1 cut(s) 470
BsuI GTATCC 1 cut(s) 25
BsuRI GGCC 1 cut(s) 79
BtsCI GGATG 3 cut(s) 126, 144, 549
BtsI GCAGTG 1 cut(s) 154
BtsIMutI CAGTG 2 cut(s) 87, 154
Cac8I GCNNGC 1 cut(s) 177
CviAII CATG 2 cut(s) 269, 374
CviJI RGCY 9 cut(s) 71, 79, 179, 227, 273, 372, 385, 390, 462
CviKI_1 RGCY 9 cut(s) 71, 79, 179, 227, 273, 372, 385, 390, 462
DpnI GATC 5 cut(s) 114, 267, 354, 472, 507
DpnII GATC 5 cut(s) 112, 265, 352, 470, 505
EaeI YGGCCR 1 cut(s) 77
Eam1104I CTCTTC 4 cut(s) 104, 280, 515, 527
EarI CTCTTC 4 cut(s) 104, 280, 515, 527
Ecl136II GAGCTC 1 cut(s) 227
Eco130I CCWWGG 2 cut(s) 214, 230
Eco24I GRGCYC 2 cut(s) 229, 464
Eco31I GGTCTC 2 cut(s) 43, 186
Eco32I GATATC 1 cut(s) 37
Eco53kI GAGCTC 1 cut(s) 227
Eco88I CYCGRG 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 227
EcoRV GATATC 1 cut(s) 37
EcoT14I CCWWGG 2 cut(s) 214, 230
EcoT38I GRGCYC 2 cut(s) 229, 464
ErhI CCWWGG 2 cut(s) 214, 230
FaeI CATG 2 cut(s) 272, 377
FaiI YATR 3 cut(s) 270, 365, 375
FaqI GGGAC 1 cut(s) 526
FatI CATG 2 cut(s) 268, 373
FbaI TGATCA 1 cut(s) 265
Fnu4HI GCNGC 1 cut(s) 383
FokI GGATG 2 cut(s) 113, 131
FriOI GRGCYC 2 cut(s) 229, 464
Fsp4HI GCNGC 1 cut(s) 383
FspBI CTAG 2 cut(s) 215, 231
GluI GCNGC 1 cut(s) 383
HaeIII GGCC 1 cut(s) 79
HapII CCGG 3 cut(s) 239, 317, 357
Hin1II CATG 2 cut(s) 272, 377
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HindIII AAGCTT 1 cut(s) 177
HinfI GANTC 7 cut(s) 5, 29, 360, 500, 526, 538, 550
HpaII CCGG 3 cut(s) 239, 317, 357
HphI GGTGA 1 cut(s) 275
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 7 cut(s) 10, 34, 466, 475, 505, 531, 543
Hpy188III TCNNGA 2 cut(s) 18, 46
Hpy8I GTNNAC 1 cut(s) 139
Hpy99I CGWCG 3 cut(s) 110, 113, 304
HpyAV CCTTC 4 cut(s) 236, 254, 391, 409
HpyCH4III ACNGT 2 cut(s) 91, 197
HpyCH4V TGCA 1 cut(s) 175
HpyF10VI GCNNNNNNNGC 2 cut(s) 165, 233
Hsp92II CATG 2 cut(s) 272, 377
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 5 cut(s) 112, 265, 352, 470, 505
LguI GCTCTTC 1 cut(s) 280
LmnI GCTCC 2 cut(s) 224, 232
LpnPI CCDG 4 cut(s) 31, 252, 330, 370
LweI GCATC 1 cut(s) 141
MaeI CTAG 2 cut(s) 215, 231
MalI GATC 5 cut(s) 114, 267, 354, 472, 507
MboI GATC 5 cut(s) 112, 265, 352, 470, 505
MflI RGATCY 1 cut(s) 470
MhlI GDGCHC 4 cut(s) 202, 229, 238, 464
MlsI TGGCCA 1 cut(s) 79
MluCI AATT 1 cut(s) 325
MluNI TGGCCA 1 cut(s) 79
MlyI GAGTC 4 cut(s) 14, 38, 369, 535
MmeI TCCRAC 2 cut(s) 465, 509
Mox20I TGGCCA 1 cut(s) 79
MscI TGGCCA 1 cut(s) 79
Msp20I TGGCCA 1 cut(s) 79
MspI CCGG 3 cut(s) 239, 317, 357
MspR9I CCNGG 1 cut(s) 357
MwoI GCNNNNNNNGC 2 cut(s) 165, 233
NciI CCSGG 1 cut(s) 357
NdeII GATC 5 cut(s) 112, 265, 352, 470, 505
NlaIII CATG 2 cut(s) 272, 377
NlaIV GGNNCC 2 cut(s) 237, 472
PciSI GCTCTTC 1 cut(s) 280
PcsI WCGNNNNNNNCGW 2 cut(s) 287, 296
PfeI GAWTC 3 cut(s) 500, 538, 550
PfoI TCCNGGA 1 cut(s) 355
PkrI GCNGC 1 cut(s) 384
PleI GAGTC 4 cut(s) 13, 37, 368, 534
PpsI GAGTC 4 cut(s) 13, 37, 368, 534
Psp124BI GAGCTC 1 cut(s) 229
PspN4I GGNNCC 2 cut(s) 237, 472
PsuI RGATCY 1 cut(s) 470
SacI GAGCTC 1 cut(s) 229
SapI GCTCTTC 1 cut(s) 280
SatI GCNGC 1 cut(s) 383
Sau3AI GATC 5 cut(s) 112, 265, 352, 470, 505
SchI GAGTC 4 cut(s) 14, 38, 369, 535
ScrFI CCNGG 1 cut(s) 357
SduI GDGCHC 4 cut(s) 202, 229, 238, 464
SfaNI GCATC 1 cut(s) 141
SmlI CTYRAG 1 cut(s) 66
SmoI CTYRAG 1 cut(s) 66
Sse9I AATT 1 cut(s) 325
SsiI CCGC 2 cut(s) 130, 382
SspMI CTAG 2 cut(s) 215, 231
SstI GAGCTC 1 cut(s) 229
StyD4I CCNGG 1 cut(s) 355
StyI CCWWGG 2 cut(s) 214, 230
TaaI ACNGT 2 cut(s) 91, 197
TaqI TCGA 5 cut(s) 105, 290, 323, 368, 553
TasI AATT 1 cut(s) 325
TauI GCSGC 1 cut(s) 385
TfiI GAWTC 3 cut(s) 500, 538, 550
TscAI CASTG 2 cut(s) 94, 161
TspDTI ATGAA 2 cut(s) 267, 507
TspRI CASTG 2 cut(s) 94, 161
XapI RAATTY 1 cut(s) 325
XmaJI CCTAGG 2 cut(s) 214, 230
XspI CTAG 2 cut(s) 215, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.