Rroxscaffold_1G00039120

Inorganic pyrophosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0022754
Physical Location & Seq
Forward (+)
56662469 .. 56665173
2705 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00039120.1

Sequence Viewer

Length: 606 bp
ATGTGGCGGCAAAGCAAGCGTGAAGAGGAATTACTAAGGCTACCGACCCCCAAAATGAAGCCCCAGGTCTCTTCCGACAAAGTCCTATCCAATTCCTCGTCATCGGAGTCGATCAACGATGAAGAGGATGAGGAAGAGCTAGAGGCCGTCGCTCGCTCCGCCAACTCCAACGATGAAGACACCGCCGAGGACAACTCCCGTGGCTCCGATGACGTGGTTGCGCCGATCGAGAATGACGATGACTTTGAAGAAGAGTCGTGGTTTGGGGCTTGGTTAGGACTCAAAGCAACATTTCGTTTGCAGATCAGTAGCTATAGTTTGATTGTCAATTTTATATTGAGGGTCAGTAGCTTTAGTTTTGTGTTTACGTTAGTTTCTTATGGGATTGGTGGTGCTATAGAAACGATTAGAGTTGTAATCGCCAAAGCATTTTTGGGCACACTTTGCCATCCCTTATGCTCTGTGTTGCCGATTGACTTGCTGGATGGAGATAGATGGGTGGTGATGGAGATGGGTCTCACCCAATTCTTCAATGAGCTTCGTTCTACTATGCCATGGAACTCACTCATGGATAGAATGATGATGGAGCTCCATTTGCAAGGATAG

Protein Analysis

201

Amino Acids

22.74

Weight (kDa)

4.36

Isoelectric Point (pI)

59.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Put_Phosphatase PF06888 162 - 201 1.1e-07 Putative Phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022754)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0041451
rosa_multiflora Rmu_sc0002584.1_g000027
rosa_roxburghii Rroxscaffold_1G00039120
rosa_wichuraiana Rw5G026190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 7, 159, 183
AgsI TTSAA 2 cut(s) 248, 532
AjiI CACGTC 1 cut(s) 214
AjnI CCWGG 1 cut(s) 63
AluBI AGCT 5 cut(s) 139, 312, 351, 538, 589
AluI AGCT 5 cut(s) 139, 312, 351, 538, 589
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 2 cut(s) 73, 521
AoxI GGCC 1 cut(s) 144
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
AspLEI GCGC 1 cut(s) 223
AsuHPI GGTGA 2 cut(s) 511, 514
BaeGI GKGCMC 1 cut(s) 440
BanII GRGCYC 1 cut(s) 591
BarI GAAGNNNNNNTAC 2 cut(s) 15, 47
BbsI GAAGAC 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 591
BccI CCATC 6 cut(s) 456, 479, 489, 499, 505, 577
BceAI ACGGC 1 cut(s) 131
BcgI CGANNNNNNTGC 2 cut(s) 460, 494
BciT130I CCWGG 1 cut(s) 65
BcoDI GTCTC 2 cut(s) 73, 521
BfaI CTAG 1 cut(s) 140
BfmI CTRYAG 2 cut(s) 313, 396
BisI GCNGC 1 cut(s) 8
BlsI GCNGC 1 cut(s) 9
Bme1390I CCNGG 1 cut(s) 65
BmgBI CACGTC 1 cut(s) 214
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 1 cut(s) 65
BpiI GAAGAC 1 cut(s) 183
BplI GAGNNNNNCTC 2 cut(s) 179, 211
BsaI GGTCTC 2 cut(s) 73, 521
BsaJI CCNNGG 4 cut(s) 63, 186, 199, 554
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 4 cut(s) 63, 186, 199, 554
BseGI GGATG 3 cut(s) 133, 448, 490
BseSI GKGCMC 1 cut(s) 440
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 1 cut(s) 146
BsiEI CGRYCG 1 cut(s) 228
BsiHKAI GWGCWC 1 cut(s) 591
BsmAI GTCTC 2 cut(s) 73, 521
BsnI GGCC 1 cut(s) 146
Bso31I GGTCTC 2 cut(s) 73, 521
Bsp1286I GDGCHC 2 cut(s) 440, 591
Bsp143I GATC 3 cut(s) 111, 225, 303
Bsp19I CCATGG 1 cut(s) 554
BspACI CCGC 3 cut(s) 7, 159, 183
BspANI GGCC 1 cut(s) 146
BspLI GGNNCC 1 cut(s) 205
BspQI GCTCTTC 1 cut(s) 129
BspTNI GGTCTC 2 cut(s) 73, 521
BssECI CCNNGG 4 cut(s) 63, 186, 199, 554
BssMI GATC 3 cut(s) 111, 225, 303
BssT1I CCWWGG 1 cut(s) 554
Bst2UI CCWGG 1 cut(s) 65
Bst6I CTCTTC 5 cut(s) 18, 76, 117, 129, 246
BstAPI GCANNNNNTGC 1 cut(s) 444
BstC8I GCNNGC 2 cut(s) 17, 154
BstDEI CTNAG 1 cut(s) 35
BstDSI CCRYGG 2 cut(s) 199, 554
BstF5I GGATG 3 cut(s) 133, 448, 490
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 3 cut(s) 114, 228, 306
BstMAI GTCTC 2 cut(s) 73, 521
BstMBI GATC 3 cut(s) 111, 225, 303
BstMCI CGRYCG 1 cut(s) 228
BstMWI GCNNNNNNNGC 4 cut(s) 16, 158, 444, 595
BstNI CCWGG 1 cut(s) 65
BstSCI CCNGG 1 cut(s) 63
BstSFI CTRYAG 2 cut(s) 313, 396
BstSLI GKGCMC 1 cut(s) 440
BstV2I GAAGAC 1 cut(s) 183
BsuRI GGCC 1 cut(s) 146
BtgI CCRYGG 2 cut(s) 199, 554
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 3 cut(s) 133, 448, 490
Cac8I GCNNGC 2 cut(s) 17, 154
CfoI GCGC 1 cut(s) 223
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviAII CATG 2 cut(s) 555, 568
DdeI CTNAG 1 cut(s) 35
DpnI GATC 3 cut(s) 113, 227, 305
DpnII GATC 3 cut(s) 111, 225, 303
Eam1104I CTCTTC 5 cut(s) 18, 76, 117, 129, 246
EarI CTCTTC 5 cut(s) 18, 76, 117, 129, 246
EciI GGCGGA 1 cut(s) 148
Ecl136II GAGCTC 1 cut(s) 589
Eco130I CCWWGG 1 cut(s) 554
Eco24I GRGCYC 1 cut(s) 591
Eco31I GGTCTC 2 cut(s) 73, 521
Eco53kI GAGCTC 1 cut(s) 589
EcoICRI GAGCTC 1 cut(s) 589
EcoRII CCWGG 1 cut(s) 63
EcoT14I CCWWGG 1 cut(s) 554
EcoT38I GRGCYC 1 cut(s) 591
ErhI CCWWGG 1 cut(s) 554
FaeI CATG 2 cut(s) 558, 571
FaiI YATR 8 cut(s) 315, 335, 381, 398, 457, 551, 556, 569
FatI CATG 2 cut(s) 554, 567
Fnu4HI GCNGC 1 cut(s) 8
FokI GGATG 3 cut(s) 140, 435, 497
FriOI GRGCYC 1 cut(s) 591
Fsp4HI GCNGC 1 cut(s) 8
FspBI CTAG 1 cut(s) 140
GlaI GCGC 1 cut(s) 222
GluI GCNGC 1 cut(s) 8
HaeIII GGCC 1 cut(s) 146
HhaI GCGC 1 cut(s) 223
Hin1II CATG 2 cut(s) 558, 571
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HinfI GANTC 3 cut(s) 107, 254, 279
HphI GGTGA 2 cut(s) 511, 514
Hpy166II GTNNAC 1 cut(s) 366
Hpy188I TCNGA 3 cut(s) 76, 106, 208
Hpy188III TCNNGA 1 cut(s) 229
Hpy8I GTNNAC 1 cut(s) 366
Hpy99I CGWCG 1 cut(s) 152
HpyCH4IV ACGT 2 cut(s) 213, 368
HpyCH4V TGCA 2 cut(s) 301, 598
HpyF10VI GCNNNNNNNGC 4 cut(s) 16, 158, 444, 595
HpyF3I CTNAG 1 cut(s) 35
HpySE526I ACGT 2 cut(s) 213, 368
Hsp92II CATG 2 cut(s) 558, 571
HspAI GCGC 1 cut(s) 221
Kzo9I GATC 3 cut(s) 111, 225, 303
LguI GCTCTTC 1 cut(s) 129
LmnI GCTCC 4 cut(s) 161, 209, 586, 594
LpnPI CCDG 3 cut(s) 50, 77, 467
MaeI CTAG 1 cut(s) 140
MaeII ACGT 2 cut(s) 213, 368
MalI GATC 3 cut(s) 113, 227, 305
MboI GATC 3 cut(s) 111, 225, 303
MboII GAAGA 8 cut(s) 35, 63, 134, 146, 188, 260, 263, 520
MhlI GDGCHC 2 cut(s) 440, 591
MluCI AATT 4 cut(s) 29, 91, 328, 524
MlyI GAGTC 3 cut(s) 116, 263, 273
MmeI TCCRAC 2 cut(s) 99, 192
MnlI CCTC 7 cut(s) 19, 106, 118, 124, 136, 181, 333
MspR9I CCNGG 1 cut(s) 65
MvaI CCWGG 1 cut(s) 65
MwoI GCNNNNNNNGC 4 cut(s) 16, 158, 444, 595
NcoI CCATGG 1 cut(s) 554
NdeII GATC 3 cut(s) 111, 225, 303
NlaIII CATG 2 cut(s) 558, 571
NlaIV GGNNCC 1 cut(s) 205
NmeAIII GCCGAG 1 cut(s) 211
PciSI GCTCTTC 1 cut(s) 129
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PflFI GACNNNGTC 1 cut(s) 80
PkrI GCNGC 1 cut(s) 9
Ple19I CGATCG 1 cut(s) 228
PleI GAGTC 3 cut(s) 115, 262, 273
PpsI GAGTC 3 cut(s) 115, 262, 273
Psp124BI GAGCTC 1 cut(s) 591
Psp6I CCWGG 1 cut(s) 63
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 205
PsyI GACNNNGTC 1 cut(s) 80
PvuI CGATCG 1 cut(s) 228
SacI GAGCTC 1 cut(s) 591
SapI GCTCTTC 1 cut(s) 129
SatI GCNGC 1 cut(s) 8
Sau3AI GATC 3 cut(s) 111, 225, 303
SchI GAGTC 3 cut(s) 116, 263, 273
ScrFI CCNGG 1 cut(s) 65
SduI GDGCHC 2 cut(s) 440, 591
SetI ASST 8 cut(s) 69, 141, 216, 314, 353, 371, 540, 591
SfcI CTRYAG 2 cut(s) 313, 396
Sse9I AATT 4 cut(s) 29, 91, 328, 524
SsiI CCGC 3 cut(s) 7, 159, 183
SspMI CTAG 1 cut(s) 140
SstI GAGCTC 1 cut(s) 591
StyD4I CCNGG 1 cut(s) 63
StyI CCWWGG 1 cut(s) 554
TaiI ACGT 2 cut(s) 216, 371
TaqI TCGA 2 cut(s) 110, 228
TasI AATT 4 cut(s) 29, 91, 328, 524
TauI GCSGC 1 cut(s) 10
TspDTI ATGAA 3 cut(s) 71, 135, 189
Tth111I GACNNNGTC 1 cut(s) 80
XcmI CCANNNNNNNNNTGG 1 cut(s) 430
XspI CTAG 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.