Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0014692

Physical Location & Seq

Genomic Coordinates Forward (+)

74095391 .. 74100941

Gene Structure 5551 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00053270.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2166 bp

ATGGCAAGACAAATGGACCCAGAGTCTGCTTCTGTTTCCGGCGGTTTACGTATGAATTTCTACGCGACCACTCTCTGCCTTGCTTATCAGAGTCTTGGTATTGTTTATGGGGATCTTAGTATATCCCCCATATATGTATACAAAAGTACATTCTCAGGTGGTATGCAACTTTATGAGGAGGATCTTGAGATTCTTGGGGTACTTTCCATGGTTTTTTGGACTTTGACTCTTATTCCTCTCTGCAAATACATTTTATTTGTGTTAGCAGCAGATGACAATGGCGAAGGTGGAACGTTTGCTTTGTATTCATCACTATGCCGACACTCAAGGATGGGTCTTTTGAACACAGTTGATCCTGCTCATGAGCGTGTATCTTCTTGCAGATCTGAAATGTCTAAGGATACAAGAATGAGCTTACTCATAAAAGAGTTTTTTGAGAAGCATAAGAGTTCTCGAATAGTATTGGTGCTTGTAGTTTTTCTAGGGATCGGCATGGTAATCGGTGATGGTATCCTCACTCCAACAATGTCTGATTATACTGTGCTCATAGCTTGCACCATCTTGGTTAGCCTTTTTGCTCTTCAGCATTATGGGACACACAGAGTTGGGTTTCTTTTTGCTCCAATCATGACAGCATGGTTGCTATGCATTAGTGGGGTGGGTATTTACAATATATTTCGTTGGAACCCTGGTGTTATATGTGCTCTGTCGCCACACTATATTTACAAGTTCTTTGAAAAAACTGGAAGAGCTGGGTGGAGTTCCCTTGGAGGCATTGTTCTTTGTCTCACAGGTACAGAAGCAATGTTTGCTGATCTTGGCCATTTTTCCAAACTCTCTATCAGGGTTGCATTTTCAGGGCTTGTTTATCCGTGCTTAATTCTGGCATACATGGGAGAAGCTGCTTATCTCTCCAAGCACAGAATGGATCTCCATAGGAGCTTTTTTAAGGCCATCCCTGAGGATGTATTTTGGCCGGTATTCATTATTGCTACTCTTGCTTCTGTGGTGGGAAGTCAAGCAATAATTTCAGCTACCTTCTCAATAGTCAGTCAGTGCAGGGGACTGCGCTGCTTCCCACGCGTGAGGATAAAACATACGTCAAATCAGATACATGGACAGATATACATTCCTGAGGTCAATTGGATTTTGATGGTATTGTGTCTTGCTGTTGTCATTGGATTTCGAGACACAAATATGATTGGCAACGCATATGGGCTTGCTGCAGTGACAGTAATGTTCATCACAACCTGCTTGATGTTTTTGATTATTAGTACTGTTTGGAAGAAAAATGTTCTGTTGGCATTCTTGTTCGTCGTAATCTTTGGATCTCTTGAGCTACTCTATATTTCTGCATGCCTCAGTAAAGTACATAAAGGAGGCTGGCTTCCCCTTTTGTTTGCTGTTGTAATCTTGTCTTTGATGTCTATATGGAATTACGGGACTGTAAAGAAAGATGCATTTGAGCTACAGAACAAGGTATCCTTGGATAGGCTGCTAAGCTCTGGGCCAAGCCTAGGTATCACAAGGGTCCCTGGAATCTGCCTAGTTTACTCTAATGTTGCCTCTGGTGTCCCTCCAATGTTTGCACATTTTGTCACAAACTTCCCAGCATTTCATCATACTCTCATATTTGTGACCCTTAAGTCTTTGATGGTTCCGAAAGTTCCAGTGGATGAGCGTTTTGTTGTTAACCGGCTTGGCCCACCAGAATTGCTTATCTTTCAGTGCATTGTAAGGTATGGATACAAGGATGTAAGAGATTGCTACGAATTTGAAACTCAACTGATTGAAGAGGTGGCAGAGTTCCTGAAACAGGAGGCCCATAGCAAAGAGATGGCAGTCAGAGAGCAGTCACCGAACTGTATGTTTGCTGCTGTGGGAAACGAGGTTAGTATTGCCAGTTCTGAGCAGTGGAGAAACGAGATTAAGGGTGGCAGTGTCGAGTGTCAGGAGGTGAACGAACTCAAGGAGGCTAGGGAAGCTGGTGTGGCATACATGATGGGAAATACTCATGTTGTGGCGAGTGACCTATCGCCTTTACTGAAGAAGTTTGCAATTGACATTGTCTACGGTTTTCTAAGACGGAATTGCAGGCGTCCAGCAATCGCCCTTCAGATTCCTCATACCTCACTGATCGAAGTGGGAATGCTTTATCAGGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

721

Amino Acids

80.31

Weight (kDa)

8.27

Isoelectric Point (pI)

38.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	27 - 501	4.4e-151	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	512 - 720	4e-27	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014692)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g15480 FvH4_3g15480
malus_domestica	MD05G1223100.v1.1 MD10G1204500.v1.1
prunus_persica	Prupe.4G137300_v2.0.a1
pyrus_communis	pycom05g20550 pycom10g17520
rosa_chinensis	RchiOBHm_Chr5g0025981
rosa_laevigata	RLG00000032888
rosa_multiflora	Rmu_sc0003020.1_g000014
rosa_roxburghii	Rroxscaffold_1G00053270
rosa_rugosa	Rorug05G0090100
rosa_samantha	Rh5AG181800 Rh5BG179900 Rh5CG198400 Rh5DG180900
rosa_wichuraiana	Rw5G016540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1645
Acc36I	ACCTGC	1 cut(s)	1259
AccI	GTMKAC	2 cut(s)	138, 2070
AccII	CGCG	2 cut(s)	65, 1083
AciI	CCGC	1 cut(s)	42
AclI	AACGTT	1 cut(s)	293
AclWI	GGATC	6 cut(s)	120, 189, 347, 494, 936, 1336
AcoI	YGGCCR	2 cut(s)	820, 974
AcsI	RAATTY	2 cut(s)	55, 1772
AcuI	CTGAAG	3 cut(s)	566, 2066, 2099
AcyI	GRCGYC	1 cut(s)	2098
AfaI	GTAC	5 cut(s)	148, 201, 796, 1276, 1371
AfiI	CCNNNNNNNGG	3 cut(s)	1491, 1517, 1816
AflII	CTTAAG	1 cut(s)	1643
AflIII	ACRYGT	1 cut(s)	1081
AgsI	TTSAA	4 cut(s)	343, 737, 1778, 1793
AhdI	GACNNNNNGTC	1 cut(s)	22
AjnI	CCWGG	2 cut(s)	688, 1534
AloI	GAACNNNNNNTCC	4 cut(s)	762, 794, 1466, 1498
Alw21I	GWGCWC	2 cut(s)	546, 706
Alw26I	GTCTC	2 cut(s)	791, 1182
AlwI	GGATC	6 cut(s)	120, 189, 347, 494, 936, 1336
AlwNI	CAGNNNCTG	1 cut(s)	26
AoxI	GGCC	6 cut(s)	820, 951, 974, 1508, 1702, 1821
ApeKI	GCWGC	6 cut(s)	266, 902, 1071, 1223, 1495, 1874
ApoI	RAATTY	2 cut(s)	55, 1772
AspA2I	CCTAGG	1 cut(s)	1516
AspLEI	GCGC	1 cut(s)	1071
AspS9I	GGNCC	5 cut(s)	16, 1508, 1531, 1703, 1822
AsuHPI	GGTGA	3 cut(s)	515, 1848, 1969
AvaII	GGWCC	2 cut(s)	16, 1531
AvrII	CCTAGG	1 cut(s)	1516
AxyI	CCTNAGG	2 cut(s)	960, 1134
BalI	TGGCCA	1 cut(s)	822
Bbv12I	GWGCWC	2 cut(s)	546, 706
BbvI	GCAGC	6 cut(s)	278, 889, 1058, 1210, 1482, 1861
BccI	CCATC	8 cut(s)	325, 500, 566, 962, 1147, 1648, 1831, 1996
BcgI	CGANNNNNNTGC	2 cut(s)	481, 515
BciT130I	CCWGG	2 cut(s)	690, 1536
BciVI	GTATCC	4 cut(s)	394, 521, 1492, 1739
BcoDI	GTCTC	2 cut(s)	791, 1182
BfaI	CTAG	4 cut(s)	482, 1517, 1547, 1977
BfmI	CTRYAG	2 cut(s)	1224, 1469
BfrI	CTTAAG	1 cut(s)	1643
BfuAI	ACCTGC	1 cut(s)	1259
BfuI	GTATCC	4 cut(s)	394, 521, 1492, 1739
BglII	AGATCT	1 cut(s)	383
BisI	GCNGC	6 cut(s)	267, 903, 1072, 1224, 1496, 1875
BlnI	CCTAGG	1 cut(s)	1516
BlpI	GCTNAGC	1 cut(s)	1499
BlsI	GCNGC	6 cut(s)	268, 904, 1073, 1225, 1497, 1876
BmcAI	AGTACT	1 cut(s)	1276
Bme1390I	CCNGG	2 cut(s)	690, 1536
Bme18I	GGWCC	2 cut(s)	16, 1531
BmeRI	GACNNNNNGTC	1 cut(s)	22
BmgT120I	GGNCC	5 cut(s)	16, 1508, 1531, 1703, 1822
BmiI	GGNNCC	5 cut(s)	18, 686, 1532, 1533, 1659
BmrFI	CCNGG	2 cut(s)	690, 1536
BmsI	GCATC	1 cut(s)	1447
Bpu1102I	GCTNAGC	1 cut(s)	1499
BpuEI	CTTGAG	4 cut(s)	206, 310, 1355, 1952
BsaAI	YACGTR	1 cut(s)	50
BsaHI	GRCGYC	1 cut(s)	2098
BsaJI	CCNNGG	6 cut(s)	207, 688, 766, 1485, 1516, 1534
BsaXI	ACNNNNNCTCC	2 cut(s)	762, 792
Bsc4I	CCNNNNNNNGG	3 cut(s)	1491, 1517, 1816
Bse118I	RCCGGY	2 cut(s)	976, 1695
Bse1I	ACTGG	3 cut(s)	748, 1670, 1902
Bse21I	CCTNAGG	2 cut(s)	960, 1134
Bse3DI	GCAATG	1 cut(s)	810
BseBI	CCWGG	2 cut(s)	690, 1536
BseDI	CCNNGG	6 cut(s)	207, 688, 766, 1485, 1516, 1534
BseGI	GGATG	5 cut(s)	336, 954, 970, 1681, 1759
BseLI	CCNNNNNNNGG	3 cut(s)	1491, 1517, 1816
BseMI	GCAATG	1 cut(s)	810
BseMII	CTCAG	5 cut(s)	168, 951, 1125, 1375, 1899
BseNI	ACTGG	3 cut(s)	748, 1670, 1902
BseRI	GAGGAG	1 cut(s)	191
BseXI	GCAGC	6 cut(s)	278, 889, 1058, 1210, 1482, 1861
BseYI	CCCAGC	2 cut(s)	752, 1609
BsgI	GTGCAG	1 cut(s)	1078
Bsh1236I	CGCG	2 cut(s)	65, 1083
BshFI	GGCC	6 cut(s)	822, 953, 976, 1510, 1704, 1823
BsiHKAI	GWGCWC	2 cut(s)	546, 706
BsiSI	CCGG	3 cut(s)	39, 977, 1696
BslFI	GGGAC	5 cut(s)	607, 1077, 1456, 1517, 1559
BslI	CCNNNNNNNGG	3 cut(s)	1491, 1517, 1816
BsmAI	GTCTC	2 cut(s)	791, 1182
BsmFI	GGGAC	5 cut(s)	607, 1077, 1456, 1517, 1559
BsmI	GAATGC	2 cut(s)	1304, 2154
BsnI	GGCC	6 cut(s)	822, 953, 976, 1510, 1704, 1823
Bsp1286I	GDGCHC	2 cut(s)	546, 706
Bsp143I	GATC	9 cut(s)	112, 181, 352, 383, 486, 814, 928, 1328, 2136
Bsp1720I	GCTNAGC	1 cut(s)	1499
Bsp19I	CCATGG	1 cut(s)	207
BspACI	CCGC	1 cut(s)	42
BspANI	GGCC	6 cut(s)	822, 953, 976, 1510, 1704, 1823
BspCNI	CTCAG	5 cut(s)	167, 952, 1126, 1374, 1900
BspFNI	CGCG	2 cut(s)	65, 1083
BspHI	TCATGA	2 cut(s)	361, 627
BspLI	GGNNCC	5 cut(s)	18, 686, 1532, 1533, 1659
BspMAI	CTGCAG	1 cut(s)	1228
BspMI	ACCTGC	1 cut(s)	1259
BspPI	GGATC	6 cut(s)	120, 189, 347, 494, 936, 1336
BspQI	GCTCTTC	2 cut(s)	585, 742
BspTI	CTTAAG	1 cut(s)	1643
BsrDI	GCAATG	1 cut(s)	810
BsrFI	RCCGGY	2 cut(s)	976, 1695
BsrI	ACTGG	3 cut(s)	748, 1670, 1902
BssAI	RCCGGY	2 cut(s)	976, 1695
BssECI	CCNNGG	6 cut(s)	207, 688, 766, 1485, 1516, 1534
BssMI	GATC	9 cut(s)	112, 181, 352, 383, 486, 814, 928, 1328, 2136
BssNAI	GTATAC	1 cut(s)	139
BssNI	GRCGYC	1 cut(s)	2098
BssT1I	CCWWGG	4 cut(s)	207, 766, 1485, 1516
Bst1107I	GTATAC	1 cut(s)	139
Bst2UI	CCWGG	2 cut(s)	690, 1536
Bst4CI	ACNGT	7 cut(s)	349, 541, 1234, 1279, 1447, 1865, 2075
Bst6I	CTCTTC	3 cut(s)	585, 742, 1788
BstACI	GRCGYC	1 cut(s)	2098
BstAFI	CTTAAG	1 cut(s)	1643
BstAPI	GCANNNNNTGC	1 cut(s)	809
BstBAI	YACGTR	1 cut(s)	50
BstC8I	GCNNGC	5 cut(s)	553, 1221, 1357, 1385, 2096
BstDEI	CTNAG	9 cut(s)	116, 154, 396, 960, 1134, 1361, 1499, 1908, 2081
BstDSI	CCRYGG	1 cut(s)	207
BstENI	CCTNNNNNAGG	2 cut(s)	1489, 1814
BstF5I	GGATG	5 cut(s)	336, 954, 970, 1681, 1759
BstFNI	CGCG	2 cut(s)	65, 1083
BstHHI	GCGC	1 cut(s)	1071
BstKTI	GATC	9 cut(s)	115, 184, 355, 386, 489, 817, 931, 1331, 2139
BstMAI	GTCTC	2 cut(s)	791, 1182
BstMBI	GATC	9 cut(s)	112, 181, 352, 383, 486, 814, 928, 1328, 2136
BstMWI	GCNNNNNNNGC	5 cut(s)	809, 998, 1080, 1982, 1991
BstNI	CCWGG	2 cut(s)	690, 1536
BstNSI	RCATGY	1 cut(s)	1359
BstSCI	CCNGG	2 cut(s)	688, 1534
BstSFI	CTRYAG	2 cut(s)	1224, 1469
BstSNI	TACGTA	1 cut(s)	50
BstUI	CGCG	2 cut(s)	65, 1083
BstV1I	GCAGC	6 cut(s)	278, 889, 1058, 1210, 1482, 1861
BstX2I	RGATCY	5 cut(s)	112, 181, 383, 928, 1328
BstYI	RGATCY	5 cut(s)	112, 181, 383, 928, 1328
BstZ17I	GTATAC	1 cut(s)	139
Bsu36I	CCTNAGG	2 cut(s)	960, 1134
BsuI	GTATCC	4 cut(s)	394, 521, 1492, 1739
BsuRI	GGCC	6 cut(s)	822, 953, 976, 1510, 1704, 1823
BtgI	CCRYGG	1 cut(s)	207
BtsCI	GGATG	5 cut(s)	336, 954, 970, 1681, 1759
BtsI	GCAGTG	3 cut(s)	1233, 1919, 1945
BtsIMutI	CAGTG	7 cut(s)	1061, 1233, 1677, 1733, 1919, 1945, 2132
BveI	ACCTGC	1 cut(s)	1259
Cac8I	GCNNGC	5 cut(s)	553, 1221, 1357, 1385, 2096
CaiI	CAGNNNCTG	1 cut(s)	26
CciI	TCATGA	2 cut(s)	361, 627
CfoI	GCGC	1 cut(s)	1071
Cfr10I	RCCGGY	2 cut(s)	976, 1695
Cfr13I	GGNCC	5 cut(s)	16, 1508, 1531, 1703, 1822
CseI	GACGC	1 cut(s)	2087
Csp6I	GTAC	5 cut(s)	147, 200, 795, 1275, 1370
CspCI	CAANNNNNGTGG	2 cut(s)	1695, 1730
CviQI	GTAC	5 cut(s)	147, 200, 795, 1275, 1370
DdeI	CTNAG	9 cut(s)	116, 154, 396, 960, 1134, 1361, 1499, 1908, 2081
DpnI	GATC	9 cut(s)	114, 183, 354, 385, 488, 816, 930, 1330, 2138
DpnII	GATC	9 cut(s)	112, 181, 352, 383, 486, 814, 928, 1328, 2136
DrdI	GACNNNNNNGTC	1 cut(s)	1645
DriI	GACNNNNNGTC	1 cut(s)	22
DseDI	GACNNNNNNGTC	1 cut(s)	1645
EaeI	YGGCCR	2 cut(s)	820, 974
Eam1104I	CTCTTC	3 cut(s)	585, 742, 1788
Eam1105I	GACNNNNNGTC	1 cut(s)	22
EarI	CTCTTC	3 cut(s)	585, 742, 1788
Eco105I	TACGTA	1 cut(s)	50
Eco130I	CCWWGG	4 cut(s)	207, 766, 1485, 1516
Eco47I	GGWCC	2 cut(s)	16, 1531
Eco57I	CTGAAG	3 cut(s)	566, 2066, 2099
Eco81I	CCTNAGG	2 cut(s)	960, 1134
EcoNI	CCTNNNNNAGG	2 cut(s)	1489, 1814
EcoO109I	RGGNCCY	1 cut(s)	1531
EcoRII	CCWGG	2 cut(s)	688, 1534
EcoT14I	CCWWGG	4 cut(s)	207, 766, 1485, 1516
EcoT22I	ATGCAT	2 cut(s)	650, 1462
ErhI	CCWWGG	4 cut(s)	207, 766, 1485, 1516
FalI	AAGNNNNNCTT	2 cut(s)	1469, 1501
FaqI	GGGAC	5 cut(s)	607, 1077, 1456, 1517, 1559
FauNDI	CATATG	1 cut(s)	1213
FblI	GTMKAC	2 cut(s)	138, 2070
Fnu4HI	GCNGC	6 cut(s)	267, 903, 1072, 1224, 1496, 1875
FokI	GGATG	5 cut(s)	343, 941, 977, 1688, 1766
Fsp4HI	GCNGC	6 cut(s)	267, 903, 1072, 1224, 1496, 1875
FspBI	CTAG	4 cut(s)	482, 1517, 1547, 1977
GlaI	GCGC	1 cut(s)	1070
GluI	GCNGC	6 cut(s)	267, 903, 1072, 1224, 1496, 1875
GsaI	CCCAGC	2 cut(s)	756, 1613
HaeIII	GGCC	6 cut(s)	822, 953, 976, 1510, 1704, 1823
HapII	CCGG	3 cut(s)	39, 977, 1696
HgaI	GACGC	1 cut(s)	2087
HhaI	GCGC	1 cut(s)	1071
Hin1I	GRCGYC	1 cut(s)	2098
Hin6I	GCGC	1 cut(s)	1069
HinP1I	GCGC	1 cut(s)	1069
HincII	GTYRAC	1 cut(s)	1693
HindII	GTYRAC	1 cut(s)	1693
HinfI	GANTC	6 cut(s)	23, 91, 190, 226, 1539, 2119
HpaI	GTTAAC	1 cut(s)	1693
HpaII	CCGG	3 cut(s)	39, 977, 1696
HphI	GGTGA	3 cut(s)	515, 1848, 1969
Hpy166II	GTNNAC	6 cut(s)	47, 139, 1552, 1693, 1960, 2071
Hpy188I	TCNGA	8 cut(s)	90, 388, 532, 1110, 1662, 1847, 1909, 2118
Hpy188III	TCNNGA	9 cut(s)	185, 362, 453, 628, 1133, 1187, 1334, 1810, 1952
Hpy8I	GTNNAC	6 cut(s)	47, 139, 1552, 1693, 1960, 2071
Hpy99I	CGWCG	1 cut(s)	1319
HpyAV	CCTTC	3 cut(s)	278, 1048, 2123
HpyCH4III	ACNGT	7 cut(s)	349, 541, 1234, 1279, 1447, 1865, 2075
HpyCH4IV	ACGT	3 cut(s)	49, 293, 1100
HpyF10VI	GCNNNNNNNGC	5 cut(s)	809, 998, 1080, 1982, 1991
HpyF3I	CTNAG	9 cut(s)	116, 154, 396, 960, 1134, 1361, 1499, 1908, 2081
HpySE526I	ACGT	3 cut(s)	49, 293, 1100
Hsp92I	GRCGYC	1 cut(s)	2098
HspAI	GCGC	1 cut(s)	1069
KflI	GGGWCCC	1 cut(s)	1531
KspAI	GTTAAC	1 cut(s)	1693
Kzo9I	GATC	9 cut(s)	112, 181, 352, 383, 486, 814, 928, 1328, 2136
LguI	GCTCTTC	2 cut(s)	585, 742
LmnI	GCTCC	2 cut(s)	625, 939
Lsp1109I	GCAGC	6 cut(s)	278, 889, 1058, 1210, 1482, 1861
LweI	GCATC	1 cut(s)	1447
MaeI	CTAG	4 cut(s)	482, 1517, 1547, 1977
MaeII	ACGT	3 cut(s)	49, 293, 1100
MaeIII	GTNAC	5 cut(s)	1228, 1597, 1636, 1854, 2027
MalI	GATC	9 cut(s)	114, 183, 354, 385, 488, 816, 930, 1330, 2138
MboI	GATC	9 cut(s)	112, 181, 352, 383, 486, 814, 928, 1328, 2136
MboII	GAAGA	6 cut(s)	366, 572, 759, 1297, 1805, 2059
MfeI	CAATTG	2 cut(s)	1141, 2058
MflI	RGATCY	5 cut(s)	112, 181, 383, 928, 1328
MhlI	GDGCHC	2 cut(s)	546, 706
MlsI	TGGCCA	1 cut(s)	822
MluCI	AATT	9 cut(s)	55, 879, 1026, 1141, 1435, 1712, 1772, 2058, 2089
MluI	ACGCGT	1 cut(s)	1081
MluNI	TGGCCA	1 cut(s)	822
MlyI	GAGTC	3 cut(s)	32, 100, 220
MmeI	TCCRAC	2 cut(s)	545, 662
Mox20I	TGGCCA	1 cut(s)	822
Mph1103I	ATGCAT	2 cut(s)	650, 1462
MscI	TGGCCA	1 cut(s)	822
MseI	TTAA	5 cut(s)	878, 948, 1644, 1692, 1929
MslI	CAYNNNNRTG	4 cut(s)	313, 366, 1196, 1634
Msp20I	TGGCCA	1 cut(s)	822
MspCI	CTTAAG	1 cut(s)	1643
MspI	CCGG	3 cut(s)	39, 977, 1696
MspR9I	CCNGG	2 cut(s)	690, 1536
MunI	CAATTG	2 cut(s)	1141, 2058
Mva1269I	GAATGC	2 cut(s)	1304, 2154
MvaI	CCWGG	2 cut(s)	690, 1536
MvnI	CGCG	2 cut(s)	65, 1083
MwoI	GCNNNNNNNGC	5 cut(s)	809, 998, 1080, 1982, 1991
NcoI	CCATGG	1 cut(s)	207
NdeI	CATATG	1 cut(s)	1213
NdeII	GATC	9 cut(s)	112, 181, 352, 383, 486, 814, 928, 1328, 2136
NlaIV	GGNNCC	5 cut(s)	18, 686, 1532, 1533, 1659
NmuCI	GTSAC	5 cut(s)	1228, 1597, 1636, 1854, 2027
NsiI	ATGCAT	2 cut(s)	650, 1462
NspI	RCATGY	1 cut(s)	1359
PaeI	GCATGC	1 cut(s)	1359
PagI	TCATGA	2 cut(s)	361, 627
PciSI	GCTCTTC	2 cut(s)	585, 742
PctI	GAATGC	2 cut(s)	1304, 2154
PfeI	GAWTC	3 cut(s)	190, 1539, 2119
PflFI	GACNNNGTC	1 cut(s)	2066
PkrI	GCNGC	6 cut(s)	268, 904, 1073, 1225, 1497, 1876
PleI	GAGTC	3 cut(s)	31, 99, 220
PpsI	GAGTC	3 cut(s)	31, 99, 220
Ppu21I	YACGTR	1 cut(s)	50
PpuMI	RGGWCCY	1 cut(s)	1531
Psp1406I	AACGTT	1 cut(s)	293
Psp5II	RGGWCCY	1 cut(s)	1531
Psp6I	CCWGG	2 cut(s)	688, 1534
PspFI	CCCAGC	2 cut(s)	752, 1609
PspGI	CCWGG	2 cut(s)	688, 1534
PspN4I	GGNNCC	5 cut(s)	18, 686, 1532, 1533, 1659
PspPI	GGNCC	5 cut(s)	16, 1508, 1531, 1703, 1822
PspPPI	RGGWCCY	1 cut(s)	1531
PsrI	GAACNNNNNNTAC	2 cut(s)	1888, 1920
PstI	CTGCAG	1 cut(s)	1228
PstNI	CAGNNNCTG	1 cut(s)	26
PsuI	RGATCY	5 cut(s)	112, 181, 383, 928, 1328
PsyI	GACNNNGTC	1 cut(s)	2066
RsaI	GTAC	5 cut(s)	148, 201, 796, 1276, 1371
RsaNI	GTAC	5 cut(s)	147, 200, 795, 1275, 1370
RseI	CAYNNNNRTG	4 cut(s)	313, 366, 1196, 1634
SapI	GCTCTTC	2 cut(s)	585, 742
SaqAI	TTAA	5 cut(s)	878, 948, 1644, 1692, 1929
SatI	GCNGC	6 cut(s)	267, 903, 1072, 1224, 1496, 1875
Sau3AI	GATC	9 cut(s)	112, 181, 352, 383, 486, 814, 928, 1328, 2136
Sau96I	GGNCC	5 cut(s)	16, 1508, 1531, 1703, 1822
ScaI	AGTACT	1 cut(s)	1276
SchI	GAGTC	3 cut(s)	32, 100, 220
ScrFI	CCNGG	2 cut(s)	690, 1536
SduI	GDGCHC	2 cut(s)	546, 706
SfaNI	GCATC	1 cut(s)	1447
SfcI	CTRYAG	2 cut(s)	1224, 1469
SinI	GGWCC	2 cut(s)	16, 1531
SmiMI	CAYNNNNRTG	4 cut(s)	313, 366, 1196, 1634
SmlI	CTYRAG	5 cut(s)	185, 325, 1334, 1643, 1967
SmoI	CTYRAG	5 cut(s)	185, 325, 1334, 1643, 1967
SnaBI	TACGTA	1 cut(s)	50
SphI	GCATGC	1 cut(s)	1359
Sse9I	AATT	9 cut(s)	55, 879, 1026, 1141, 1435, 1712, 1772, 2058, 2089
SsiI	CCGC	1 cut(s)	42
SspMI	CTAG	4 cut(s)	482, 1517, 1547, 1977
StyD4I	CCNGG	2 cut(s)	688, 1534
StyI	CCWWGG	4 cut(s)	207, 766, 1485, 1516
TaaI	ACNGT	7 cut(s)	349, 541, 1234, 1279, 1447, 1865, 2075
TaiI	ACGT	3 cut(s)	52, 296, 1103
TaqI	TCGA	4 cut(s)	454, 1186, 1944, 2139
TasI	AATT	9 cut(s)	55, 879, 1026, 1141, 1435, 1712, 1772, 2058, 2089
TatI	WGTACW	3 cut(s)	146, 1274, 1369
TfiI	GAWTC	3 cut(s)	190, 1539, 2119
Tru1I	TTAA	5 cut(s)	878, 948, 1644, 1692, 1929
Tru9I	TTAA	5 cut(s)	878, 948, 1644, 1692, 1929
TscAI	CASTG	7 cut(s)	1061, 1233, 1677, 1733, 1919, 1945, 2139
TseFI	GTSAC	5 cut(s)	1228, 1597, 1636, 1854, 2027
TseI	GCWGC	6 cut(s)	266, 902, 1071, 1223, 1495, 1874
Tsp45I	GTSAC	5 cut(s)	1228, 1597, 1636, 1854, 2027
TspDTI	ATGAA	5 cut(s)	68, 297, 973, 1231, 1607
TspGWI	ACGGA	2 cut(s)	861, 2101
TspRI	CASTG	7 cut(s)	1061, 1233, 1677, 1733, 1919, 1945, 2139
Tth111I	GACNNNGTC	1 cut(s)	2066
Vha464I	CTTAAG	1 cut(s)	1643
VpaK11BI	GGWCC	2 cut(s)	16, 1531
XagI	CCTNNNNNAGG	2 cut(s)	1489, 1814
XapI	RAATTY	2 cut(s)	55, 1772
XceI	RCATGY	1 cut(s)	1359
XcmI	CCANNNNNNNNNTGG	1 cut(s)	922
XmaJI	CCTAGG	1 cut(s)	1516
XmiI	GTMKAC	2 cut(s)	138, 2070
XspI	CTAG	4 cut(s)	482, 1517, 1547, 1977
ZrmI	AGTACT	1 cut(s)	1276
Zsp2I	ATGCAT	2 cut(s)	650, 1462

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.