Rh5CG198400

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0014692
Physical Location & Seq
Reverse (-)
19847506 .. 19852742
5237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG198400.1

Sequence Viewer

Length: 2217 bp
ATGGCAAGACAAATGGACCCAGAATCTGCTTCTGTTTCCGGCGGTTTACGTATGAATTTCTACGCGACCACTCTCTGCCTTGCTTATCAGAGTCTTGGTATTGTTTATGGGGATCTTAGTATATCCCCCATATATGTATACAAAAGTGCATTCTCAGGTGGTATGCAACTTTATGAGGAGGATCTTGAGATTCTTGGGGTACTTTCCATGGTTTTTTGGACTTTGACTCTTATTCCTCTCTGCAAATACATTTTATTTGTGTTAGCAGCAGATGACAATGGCGAAGGTGGAACGTTTGCTTTGTATTCATCACTATGCCGACACTCAAGGATGGGTCTTTTGAACACAGTTGATCCTGCTCATGAGCGTGTATCTTCTTGCAGATCTGAAACGTCTAAGGATACAAGAATGAGCTTACTCATAAAAGAGTTTTTTGAGAAGCATAAGAGTTCTCAAATAGTATTGGTGCTTGTAGTTTTTCTAGGGATCGGCATGGTAATCGGTGATGGTATCCTCACTCCAACAATGTCTGTTCTTTCTGCAGTCAATGGAATTAAAGTCGAAGTCCCAGACTTACATGAGAATTATACTGTGCTCATAGCTTGCACCATCTTGGTTAGCCTTTTTGCTCTTCAGCATTATGGGACACACAGAGTTGGGTTTCTTTTTGCTCCAATCATGACAGCATGGTTGCTATGCATTAGTGGGGTGGGCATTTACAATATATTTCGTTGGAACCCTGGTGTTATATGTGCTCTGTCGCCACACTATATTTACAAGTTCTTTGAAAAAACTGGAAGAGCTGGGTGGAGTTCCCTTGGAGGCATTGTTCTTTGTCTCACAGGTACAGAAGCAATGTTTGCTGATCTTGGCCATTTTTCCAAACTCTCTATCAGGGTTGCATTTTCAGGGCTTGTTTATCCGTGCTTAGTTCTGGCATACATGGGAGAAGCTGCTTATCTCTCCAAGCACAGAATGGATCTCCATAGAAGCTTTTTCAAGGCCATCCCTGAGGATGTATTTTGGCCAGTATTCATTATTGCTACTCTTGCTTCTGTGGTGGGAAGTCAAGCAATAATTTCAGCTACCTTCTCAATAGTCAGTCAGTGCAGGGGACTGCGCTGCTTCCCACGCGTGAGGATAAAACATACATCAAATCAGATACATGGGCAGATATACATTCCTGAGGTCAATTGGATTTTGATGGTATTGTGTCTTGCTGTTGTCATTGGATTTCGAGACACTAATATGATTGGCAACGCATATGGGCTTGCTGCAGTGACAGTAATGTTCATCACGACCTGCTTGATGTTTTTTATTATTAGTACTGTTTGGAAGAAAAATGTTCTGTTGGCATTCTTGTTCGTCGTAATCTTTGGATCTCTTGAGCTACTCTATATTTCTGCATGCCTCAGTAAAGTACATAAAGGAGGCTGGCTTCCCCTTTTATTTGCTGTTGTAATCTTGTCTTTGATGTCTATATGGAATTACGGGACTGTAAAGAAAGATGCATTTGAGCTACAGAACAAGGTATCCTTGGATAGGCTGCTAAGCTCTGGGCCGAGCCTAGGTATCAAAAGGGTCCCTGGAATCTGCCTAGTTTACTCTAACGTTGCCTCTGGTGTCCCTCCAATGTTTGCACATTTTGTCACAAACTTCCCAGCATTTCATCATACTCTCATATTTGTGACCCTTAAGTCTTTGATGGTACCGAAAGTTCCAGTGGATGAGCGTTTTGTTGTTAACCGGGTTGGCCCACCAGAATTGCTTATCTTTCGGTGTATTGTAAGGTATGGATACAAGGATGTAAGAGATTGCTACAAATTTGAAACTCAACTGATTGAAGAGGTGGCAGAGTTCCTGAAACAGGAGGCCCATAGTAAAGAGATGGCAGTCAGAGGGCAGTCACCGAACTTTATGTTTGCTGCTGTGGGAAACGAGGTTAGTATTGCCAGTTCTGAGCAGTGGAGAAACGAGATTAAGGGTGGCAGTGTCGAGTGTCAGAAGGTGAACGAACTCAAGGAGGCTAGGGAAGCTGGTGTCGCATACATGATGGGAAATACTCATGTTGTGGCGAGTGACCTATCGCCTTTCCTGAAGAAGTTTGCAATTGACATTGTCTACGGTTTTCTAAGACGGAATTGCAGGCGTCCAGCAATCGCCCTTCAGATTCCTCATACCTCACTGATTGAAGTGGGAATGCTTTATCAGGTGTAA

Protein Analysis

738

Amino Acids

82.09

Weight (kDa)

8.69

Isoelectric Point (pI)

36.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K_trans PF02705 27 - 518 5e-165 K+ potassium transporter integral membrane domain
K_trans_C PF22776 529 - 737 1e-27 K+ potassium transporter C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014692)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g15480 FvH4_3g15480
malus_domestica MD05G1223100.v1.1 MD10G1204500.v1.1
prunus_persica Prupe.4G137300_v2.0.a1
pyrus_communis pycom05g20550 pycom10g17520
rosa_chinensis RchiOBHm_Chr5g0025981
rosa_laevigata RLG00000032888
rosa_multiflora Rmu_sc0003020.1_g000014
rosa_roxburghii Rroxscaffold_1G00053270
rosa_rugosa Rorug05G0090100
rosa_samantha Rh5AG181800 Rh5BG179900 Rh5CG198400 Rh5DG180900
rosa_wichuraiana Rw5G016540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1696
Acc36I ACCTGC 1 cut(s) 1310
Acc65I GGTACC 1 cut(s) 1708
AccB1I GGYRCC 1 cut(s) 1708
AccI GTMKAC 2 cut(s) 138, 2121
AccII CGCG 2 cut(s) 65, 1134
AciI CCGC 1 cut(s) 42
AclI AACGTT 2 cut(s) 293, 1611
AclWI GGATC 6 cut(s) 120, 189, 347, 494, 987, 1387
AcoI YGGCCR 2 cut(s) 871, 1025
AcsI RAATTY 2 cut(s) 55, 1823
AcuI CTGAAG 3 cut(s) 617, 2117, 2150
AcyI GRCGYC 1 cut(s) 2149
AfaI GTAC 5 cut(s) 201, 847, 1327, 1422, 1710
AfiI CCNNNNNNNGG 3 cut(s) 1542, 1568, 1867
AflII CTTAAG 1 cut(s) 1694
AflIII ACRYGT 1 cut(s) 1132
AgsI TTSAA 6 cut(s) 343, 788, 1000, 1829, 1844, 2192
AjnI CCWGG 2 cut(s) 739, 1585
AloI GAACNNNNNNTCC 4 cut(s) 813, 845, 1517, 1549
Alw21I GWGCWC 2 cut(s) 597, 757
Alw26I GTCTC 2 cut(s) 842, 1233
AlwI GGATC 6 cut(s) 120, 189, 347, 494, 987, 1387
AlwNI CAGNNNCTG 1 cut(s) 26
AoxI GGCC 6 cut(s) 871, 1002, 1025, 1559, 1753, 1872
ApeKI GCWGC 6 cut(s) 266, 953, 1122, 1274, 1546, 1925
ApoI RAATTY 2 cut(s) 55, 1823
Asp718I GGTACC 1 cut(s) 1708
AspA2I CCTAGG 1 cut(s) 1567
AspLEI GCGC 1 cut(s) 1122
AspS9I GGNCC 5 cut(s) 16, 1559, 1582, 1754, 1873
AsuC2I CCSGG 1 cut(s) 1748
AsuHPI GGTGA 3 cut(s) 515, 1899, 2020
AvaII GGWCC 2 cut(s) 16, 1582
AvrII CCTAGG 1 cut(s) 1567
AxyI CCTNAGG 2 cut(s) 1011, 1185
BalI TGGCCA 2 cut(s) 873, 1027
BanI GGYRCC 1 cut(s) 1708
Bbv12I GWGCWC 2 cut(s) 597, 757
BbvI GCAGC 6 cut(s) 278, 940, 1109, 1261, 1533, 1912
BccI CCATC 8 cut(s) 325, 500, 617, 1013, 1198, 1699, 1882, 2047
BcgI CGANNNNNNTGC 2 cut(s) 481, 515
BciT130I CCWGG 2 cut(s) 741, 1587
BciVI GTATCC 4 cut(s) 394, 521, 1543, 1790
BcnI CCSGG 1 cut(s) 1748
BcoDI GTCTC 2 cut(s) 842, 1233
BfaI CTAG 4 cut(s) 482, 1568, 1598, 2028
BfmI CTRYAG 3 cut(s) 540, 1275, 1520
BfrI CTTAAG 1 cut(s) 1694
BfuAI ACCTGC 1 cut(s) 1310
BfuI GTATCC 4 cut(s) 394, 521, 1543, 1790
BglII AGATCT 1 cut(s) 383
BisI GCNGC 6 cut(s) 267, 954, 1123, 1275, 1547, 1926
BlnI CCTAGG 1 cut(s) 1567
BlpI GCTNAGC 1 cut(s) 1550
BlsI GCNGC 6 cut(s) 268, 955, 1124, 1276, 1548, 1927
BmcAI AGTACT 1 cut(s) 1327
Bme1390I CCNGG 3 cut(s) 741, 1587, 1748
Bme18I GGWCC 2 cut(s) 16, 1582
BmgT120I GGNCC 5 cut(s) 16, 1559, 1582, 1754, 1873
BmiI GGNNCC 5 cut(s) 18, 737, 1583, 1584, 1710
BmrFI CCNGG 3 cut(s) 741, 1587, 1748
BmsI GCATC 1 cut(s) 1498
Bpu1102I GCTNAGC 1 cut(s) 1550
BpuEI CTTGAG 4 cut(s) 206, 310, 1406, 2003
BpuMI CCSGG 1 cut(s) 1748
BsaAI YACGTR 1 cut(s) 50
BsaHI GRCGYC 1 cut(s) 2149
BsaJI CCNNGG 6 cut(s) 207, 739, 817, 1536, 1567, 1585
BsaXI ACNNNNNCTCC 2 cut(s) 813, 843
Bsc4I CCNNNNNNNGG 3 cut(s) 1542, 1568, 1867
Bse1I ACTGG 4 cut(s) 799, 1028, 1721, 1953
Bse21I CCTNAGG 2 cut(s) 1011, 1185
Bse3DI GCAATG 1 cut(s) 861
BseBI CCWGG 2 cut(s) 741, 1587
BseDI CCNNGG 6 cut(s) 207, 739, 817, 1536, 1567, 1585
BseGI GGATG 5 cut(s) 336, 1005, 1021, 1732, 1810
BseLI CCNNNNNNNGG 3 cut(s) 1542, 1568, 1867
BseMI GCAATG 1 cut(s) 861
BseMII CTCAG 5 cut(s) 168, 1002, 1176, 1426, 1950
BseNI ACTGG 4 cut(s) 799, 1028, 1721, 1953
BseRI GAGGAG 1 cut(s) 191
BseXI GCAGC 6 cut(s) 278, 940, 1109, 1261, 1533, 1912
BseYI CCCAGC 2 cut(s) 803, 1660
BsgI GTGCAG 1 cut(s) 1129
Bsh1236I CGCG 2 cut(s) 65, 1134
BshFI GGCC 6 cut(s) 873, 1004, 1027, 1561, 1755, 1874
BshNI GGYRCC 1 cut(s) 1708
BsiHKAI GWGCWC 2 cut(s) 597, 757
BsiSI CCGG 2 cut(s) 39, 1747
BslFI GGGAC 6 cut(s) 551, 658, 1128, 1507, 1568, 1610
BslI CCNNNNNNNGG 3 cut(s) 1542, 1568, 1867
BsmAI GTCTC 2 cut(s) 842, 1233
BsmFI GGGAC 6 cut(s) 551, 658, 1128, 1507, 1568, 1610
BsmI GAATGC 3 cut(s) 149, 1355, 2205
BsnI GGCC 6 cut(s) 873, 1004, 1027, 1561, 1755, 1874
Bsp1286I GDGCHC 2 cut(s) 597, 757
Bsp143I GATC 8 cut(s) 112, 181, 352, 383, 486, 865, 979, 1379
Bsp1720I GCTNAGC 1 cut(s) 1550
Bsp19I CCATGG 1 cut(s) 207
BspACI CCGC 1 cut(s) 42
BspANI GGCC 6 cut(s) 873, 1004, 1027, 1561, 1755, 1874
BspCNI CTCAG 5 cut(s) 167, 1003, 1177, 1425, 1951
BspFNI CGCG 2 cut(s) 65, 1134
BspHI TCATGA 2 cut(s) 361, 678
BspLI GGNNCC 5 cut(s) 18, 737, 1583, 1584, 1710
BspMAI CTGCAG 2 cut(s) 544, 1279
BspMI ACCTGC 1 cut(s) 1310
BspPI GGATC 6 cut(s) 120, 189, 347, 494, 987, 1387
BspQI GCTCTTC 2 cut(s) 636, 793
BspT107I GGYRCC 1 cut(s) 1708
BspTI CTTAAG 1 cut(s) 1694
BsrDI GCAATG 1 cut(s) 861
BsrI ACTGG 4 cut(s) 799, 1028, 1721, 1953
BssECI CCNNGG 6 cut(s) 207, 739, 817, 1536, 1567, 1585
BssMI GATC 8 cut(s) 112, 181, 352, 383, 486, 865, 979, 1379
BssNAI GTATAC 1 cut(s) 139
BssNI GRCGYC 1 cut(s) 2149
BssT1I CCWWGG 4 cut(s) 207, 817, 1536, 1567
Bst1107I GTATAC 1 cut(s) 139
Bst2UI CCWGG 2 cut(s) 741, 1587
Bst4CI ACNGT 6 cut(s) 349, 592, 1285, 1330, 1498, 2126
Bst6I CTCTTC 3 cut(s) 636, 793, 1839
BstACI GRCGYC 1 cut(s) 2149
BstAFI CTTAAG 1 cut(s) 1694
BstAPI GCANNNNNTGC 1 cut(s) 860
BstBAI YACGTR 1 cut(s) 50
BstC8I GCNNGC 5 cut(s) 604, 1272, 1408, 1436, 2147
BstDSI CCRYGG 1 cut(s) 207
BstENI CCTNNNNNAGG 2 cut(s) 1540, 1865
BstF5I GGATG 5 cut(s) 336, 1005, 1021, 1732, 1810
BstFNI CGCG 2 cut(s) 65, 1134
BstHHI GCGC 1 cut(s) 1122
BstKTI GATC 8 cut(s) 115, 184, 355, 386, 489, 868, 982, 1382
BstMAI GTCTC 2 cut(s) 842, 1233
BstMBI GATC 8 cut(s) 112, 181, 352, 383, 486, 865, 979, 1379
BstMWI GCNNNNNNNGC 5 cut(s) 860, 1049, 1131, 2033, 2042
BstNI CCWGG 2 cut(s) 741, 1587
BstNSI RCATGY 1 cut(s) 1410
BstSCI CCNGG 3 cut(s) 739, 1585, 1746
BstSFI CTRYAG 3 cut(s) 540, 1275, 1520
BstSNI TACGTA 1 cut(s) 50
BstUI CGCG 2 cut(s) 65, 1134
BstV1I GCAGC 6 cut(s) 278, 940, 1109, 1261, 1533, 1912
BstX2I RGATCY 5 cut(s) 112, 181, 383, 979, 1379
BstYI RGATCY 5 cut(s) 112, 181, 383, 979, 1379
BstZ17I GTATAC 1 cut(s) 139
Bsu36I CCTNAGG 2 cut(s) 1011, 1185
BsuI GTATCC 4 cut(s) 394, 521, 1543, 1790
BsuRI GGCC 6 cut(s) 873, 1004, 1027, 1561, 1755, 1874
BtgI CCRYGG 1 cut(s) 207
BtsCI GGATG 5 cut(s) 336, 1005, 1021, 1732, 1810
BtsI GCAGTG 3 cut(s) 1284, 1970, 1996
BtsIMutI CAGTG 6 cut(s) 1112, 1284, 1728, 1970, 1996, 2183
BveI ACCTGC 1 cut(s) 1310
Cac8I GCNNGC 5 cut(s) 604, 1272, 1408, 1436, 2147
CaiI CAGNNNCTG 1 cut(s) 26
CciI TCATGA 2 cut(s) 361, 678
CfoI GCGC 1 cut(s) 1122
Cfr13I GGNCC 5 cut(s) 16, 1559, 1582, 1754, 1873
CseI GACGC 1 cut(s) 2138
Csp6I GTAC 5 cut(s) 200, 846, 1326, 1421, 1709
CspCI CAANNNNNGTGG 2 cut(s) 1746, 1781
CviQI GTAC 5 cut(s) 200, 846, 1326, 1421, 1709
DpnI GATC 8 cut(s) 114, 183, 354, 385, 488, 867, 981, 1381
DpnII GATC 8 cut(s) 112, 181, 352, 383, 486, 865, 979, 1379
DrdI GACNNNNNNGTC 1 cut(s) 1696
DseDI GACNNNNNNGTC 1 cut(s) 1696
EaeI YGGCCR 2 cut(s) 871, 1025
Eam1104I CTCTTC 3 cut(s) 636, 793, 1839
EarI CTCTTC 3 cut(s) 636, 793, 1839
Eco105I TACGTA 1 cut(s) 50
Eco130I CCWWGG 4 cut(s) 207, 817, 1536, 1567
Eco47I GGWCC 2 cut(s) 16, 1582
Eco57I CTGAAG 3 cut(s) 617, 2117, 2150
Eco81I CCTNAGG 2 cut(s) 1011, 1185
EcoNI CCTNNNNNAGG 2 cut(s) 1540, 1865
EcoO109I RGGNCCY 1 cut(s) 1582
EcoRII CCWGG 2 cut(s) 739, 1585
EcoT14I CCWWGG 4 cut(s) 207, 817, 1536, 1567
EcoT22I ATGCAT 2 cut(s) 701, 1513
ErhI CCWWGG 4 cut(s) 207, 817, 1536, 1567
FalI AAGNNNNNCTT 2 cut(s) 1520, 1552
FaqI GGGAC 6 cut(s) 551, 658, 1128, 1507, 1568, 1610
FauNDI CATATG 1 cut(s) 1264
FblI GTMKAC 2 cut(s) 138, 2121
Fnu4HI GCNGC 6 cut(s) 267, 954, 1123, 1275, 1547, 1926
FokI GGATG 5 cut(s) 343, 992, 1028, 1739, 1817
Fsp4HI GCNGC 6 cut(s) 267, 954, 1123, 1275, 1547, 1926
FspBI CTAG 4 cut(s) 482, 1568, 1598, 2028
GlaI GCGC 1 cut(s) 1121
GluI GCNGC 6 cut(s) 267, 954, 1123, 1275, 1547, 1926
GsaI CCCAGC 2 cut(s) 807, 1664
HaeIII GGCC 6 cut(s) 873, 1004, 1027, 1561, 1755, 1874
HapII CCGG 2 cut(s) 39, 1747
HgaI GACGC 1 cut(s) 2138
HhaI GCGC 1 cut(s) 1122
Hin1I GRCGYC 1 cut(s) 2149
Hin6I GCGC 1 cut(s) 1120
HinP1I GCGC 1 cut(s) 1120
HincII GTYRAC 1 cut(s) 1744
HindII GTYRAC 1 cut(s) 1744
HindIII AAGCTT 1 cut(s) 991
HinfI GANTC 6 cut(s) 23, 91, 190, 226, 1590, 2170
HpaI GTTAAC 1 cut(s) 1744
HpaII CCGG 2 cut(s) 39, 1747
HphI GGTGA 3 cut(s) 515, 1899, 2020
Hpy166II GTNNAC 6 cut(s) 47, 139, 1603, 1744, 2011, 2122
Hpy188I TCNGA 7 cut(s) 90, 388, 1161, 1898, 1960, 2004, 2169
Hpy188III TCNNGA 9 cut(s) 185, 362, 679, 1184, 1238, 1297, 1385, 1861, 2095
Hpy8I GTNNAC 6 cut(s) 47, 139, 1603, 1744, 2011, 2122
Hpy99I CGWCG 1 cut(s) 1370
HpyAV CCTTC 4 cut(s) 278, 1099, 1999, 2174
HpyCH4III ACNGT 6 cut(s) 349, 592, 1285, 1330, 1498, 2126
HpyCH4IV ACGT 4 cut(s) 49, 293, 392, 1611
HpyF10VI GCNNNNNNNGC 5 cut(s) 860, 1049, 1131, 2033, 2042
HpySE526I ACGT 4 cut(s) 49, 293, 392, 1611
Hsp92I GRCGYC 1 cut(s) 2149
HspAI GCGC 1 cut(s) 1120
KflI GGGWCCC 1 cut(s) 1582
KpnI GGTACC 1 cut(s) 1712
KspAI GTTAAC 1 cut(s) 1744
Kzo9I GATC 8 cut(s) 112, 181, 352, 383, 486, 865, 979, 1379
LguI GCTCTTC 2 cut(s) 636, 793
LmnI GCTCC 1 cut(s) 676
Lsp1109I GCAGC 6 cut(s) 278, 940, 1109, 1261, 1533, 1912
LweI GCATC 1 cut(s) 1498
MaeI CTAG 4 cut(s) 482, 1568, 1598, 2028
MaeII ACGT 4 cut(s) 49, 293, 392, 1611
MaeIII GTNAC 5 cut(s) 1279, 1648, 1687, 1905, 2078
MalI GATC 8 cut(s) 114, 183, 354, 385, 488, 867, 981, 1381
MboI GATC 8 cut(s) 112, 181, 352, 383, 486, 865, 979, 1379
MboII GAAGA 6 cut(s) 366, 623, 810, 1348, 1856, 2110
MfeI CAATTG 2 cut(s) 1192, 2109
MflI RGATCY 5 cut(s) 112, 181, 383, 979, 1379
MhlI GDGCHC 2 cut(s) 597, 757
MlsI TGGCCA 2 cut(s) 873, 1027
MluI ACGCGT 1 cut(s) 1132
MluNI TGGCCA 2 cut(s) 873, 1027
MlyI GAGTC 2 cut(s) 100, 220
MmeI TCCRAC 2 cut(s) 545, 713
Mox20I TGGCCA 2 cut(s) 873, 1027
Mph1103I ATGCAT 2 cut(s) 701, 1513
MscI TGGCCA 2 cut(s) 873, 1027
MseI TTAA 4 cut(s) 555, 1695, 1743, 1980
MslI CAYNNNNRTG 4 cut(s) 313, 366, 1247, 1685
Msp20I TGGCCA 2 cut(s) 873, 1027
MspCI CTTAAG 1 cut(s) 1694
MspI CCGG 2 cut(s) 39, 1747
MspR9I CCNGG 3 cut(s) 741, 1587, 1748
MunI CAATTG 2 cut(s) 1192, 2109
Mva1269I GAATGC 3 cut(s) 149, 1355, 2205
MvaI CCWGG 2 cut(s) 741, 1587
MvnI CGCG 2 cut(s) 65, 1134
MwoI GCNNNNNNNGC 5 cut(s) 860, 1049, 1131, 2033, 2042
NciI CCSGG 1 cut(s) 1748
NcoI CCATGG 1 cut(s) 207
NdeI CATATG 1 cut(s) 1264
NdeII GATC 8 cut(s) 112, 181, 352, 383, 486, 865, 979, 1379
NlaIV GGNNCC 5 cut(s) 18, 737, 1583, 1584, 1710
NmeAIII GCCGAG 1 cut(s) 1587
NmuCI GTSAC 5 cut(s) 1279, 1648, 1687, 1905, 2078
NsiI ATGCAT 2 cut(s) 701, 1513
NspI RCATGY 1 cut(s) 1410
PaeI GCATGC 1 cut(s) 1410
PagI TCATGA 2 cut(s) 361, 678
PciSI GCTCTTC 2 cut(s) 636, 793
PctI GAATGC 3 cut(s) 149, 1355, 2205
PfeI GAWTC 4 cut(s) 23, 190, 1590, 2170
PflFI GACNNNGTC 1 cut(s) 2117
PkrI GCNGC 6 cut(s) 268, 955, 1124, 1276, 1548, 1927
PleI GAGTC 2 cut(s) 99, 220
PpsI GAGTC 2 cut(s) 99, 220
Ppu21I YACGTR 1 cut(s) 50
PpuMI RGGWCCY 1 cut(s) 1582
Psp1406I AACGTT 2 cut(s) 293, 1611
Psp5II RGGWCCY 1 cut(s) 1582
Psp6I CCWGG 2 cut(s) 739, 1585
PspFI CCCAGC 2 cut(s) 803, 1660
PspGI CCWGG 2 cut(s) 739, 1585
PspN4I GGNNCC 5 cut(s) 18, 737, 1583, 1584, 1710
PspPI GGNCC 5 cut(s) 16, 1559, 1582, 1754, 1873
PspPPI RGGWCCY 1 cut(s) 1582
PsrI GAACNNNNNNTAC 4 cut(s) 1701, 1733, 1939, 1971
PstI CTGCAG 2 cut(s) 544, 1279
PstNI CAGNNNCTG 1 cut(s) 26
PsuI RGATCY 5 cut(s) 112, 181, 383, 979, 1379
PsyI GACNNNGTC 1 cut(s) 2117
RsaI GTAC 5 cut(s) 201, 847, 1327, 1422, 1710
RsaNI GTAC 5 cut(s) 200, 846, 1326, 1421, 1709
RseI CAYNNNNRTG 4 cut(s) 313, 366, 1247, 1685
SapI GCTCTTC 2 cut(s) 636, 793
SaqAI TTAA 4 cut(s) 555, 1695, 1743, 1980
SatI GCNGC 6 cut(s) 267, 954, 1123, 1275, 1547, 1926
Sau3AI GATC 8 cut(s) 112, 181, 352, 383, 486, 865, 979, 1379
Sau96I GGNCC 5 cut(s) 16, 1559, 1582, 1754, 1873
ScaI AGTACT 1 cut(s) 1327
SchI GAGTC 2 cut(s) 100, 220
ScrFI CCNGG 3 cut(s) 741, 1587, 1748
SduI GDGCHC 2 cut(s) 597, 757
SfaNI GCATC 1 cut(s) 1498
SfcI CTRYAG 3 cut(s) 540, 1275, 1520
SinI GGWCC 2 cut(s) 16, 1582
SmiMI CAYNNNNRTG 4 cut(s) 313, 366, 1247, 1685
SmlI CTYRAG 5 cut(s) 185, 325, 1385, 1694, 2018
SmoI CTYRAG 5 cut(s) 185, 325, 1385, 1694, 2018
SnaBI TACGTA 1 cut(s) 50
SphI GCATGC 1 cut(s) 1410
SsiI CCGC 1 cut(s) 42
SspMI CTAG 4 cut(s) 482, 1568, 1598, 2028
StyD4I CCNGG 3 cut(s) 739, 1585, 1746
StyI CCWWGG 4 cut(s) 207, 817, 1536, 1567
TaaI ACNGT 6 cut(s) 349, 592, 1285, 1330, 1498, 2126
TaiI ACGT 4 cut(s) 52, 296, 395, 1614
TaqI TCGA 3 cut(s) 561, 1237, 1995
TatI WGTACW 2 cut(s) 1325, 1420
TfiI GAWTC 4 cut(s) 23, 190, 1590, 2170
Tru1I TTAA 4 cut(s) 555, 1695, 1743, 1980
Tru9I TTAA 4 cut(s) 555, 1695, 1743, 1980
TscAI CASTG 6 cut(s) 1112, 1284, 1728, 1970, 1996, 2190
TseFI GTSAC 5 cut(s) 1279, 1648, 1687, 1905, 2078
TseI GCWGC 6 cut(s) 266, 953, 1122, 1274, 1546, 1925
Tsp45I GTSAC 5 cut(s) 1279, 1648, 1687, 1905, 2078
TspDTI ATGAA 5 cut(s) 68, 297, 1024, 1282, 1658
TspGWI ACGGA 2 cut(s) 912, 2152
TspRI CASTG 6 cut(s) 1112, 1284, 1728, 1970, 1996, 2190
Tth111I GACNNNGTC 1 cut(s) 2117
Vha464I CTTAAG 1 cut(s) 1694
VpaK11BI GGWCC 2 cut(s) 16, 1582
XagI CCTNNNNNAGG 2 cut(s) 1540, 1865
XapI RAATTY 2 cut(s) 55, 1823
XceI RCATGY 1 cut(s) 1410
XcmI CCANNNNNNNNNTGG 1 cut(s) 973
XmaJI CCTAGG 1 cut(s) 1567
XmiI GTMKAC 2 cut(s) 138, 2121
XspI CTAG 4 cut(s) 482, 1568, 1598, 2028
ZrmI AGTACT 1 cut(s) 1327
Zsp2I ATGCAT 2 cut(s) 701, 1513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.