Rroxscaffold_1G00054500

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0025013
Physical Location & Seq
Reverse (-)
75882067 .. 75885608
3542 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_1G00054500.1

Sequence Viewer

Length: 813 bp
ATGGATCGACCAAGATGGCCGGTTCTGTGGCAAAGTTTTGATTATCCAGGTGATGTACTTCTGCCAGGCATGAAACTAGGGGTTGACCATAGTAGTGGCCACATTTGGTCACTTTCGTCACAGTTAACTGAAAAAAGTGCAATGCCAGGAGCTTTCACTCTTGATTGGGATCCTGATAGACTTGAAATAAAAATTAGACTACGAGGGGTAGTTAATTGGGGTAGTGGAGTCTTCAGAGATGGAAGTTTTGAGTTTCTTAAGCAGAAGAGCTATCTATTAAGAAGAGATCCTGCCCCTCCAGGGCCTCCAAATAATGGATCAATGAGAGGTGATTTAAGAGTATTTAGCTATGACTCTGTCATGGCTGCTACAGACAATTTCTCTGCAGAAAACAAGCTCGGAGAAGGGGGCTTTGGACCTGTTTATAAGGGATCAATGCCGACAGGTCAAGAAATAGCTGTAAAGACGCTTTCAAGAGGGTGGGCCCTGCCTCCCTCCGCCCCTGCGTGGGAGTTATGGCAAGAAGGTGTAGGACTAGAATTGATGGACGCAACTCTAAGTGATTCGTGTGTTGAAAATCAATTTTTAAGATGCATCCATGTTGTTCTGCTATGCGTCGAAGAAGATGCAGAACTTTGGCCAACCATGTCAGATGCTATATATATGCTGACAAATGAAAGCTTGCCTTTACCTATACCAACAAGACCAGCATCTTTTCCAGTCAGGATTTCGGCTGGAGTAAATATAAGAGGAAATGACTCTGAAAAGATATCAGAAAATGGTTTGTCCGACTCCCTTATTGTAGGACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr

Protein Analysis

270

Amino Acids

29.78

Weight (kDa)

4.92

Isoelectric Point (pI)

51.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 8 - 40 4.4e-08 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0025013)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0024961
rosa_laevigata RLG00000032825
rosa_roxburghii Rroxscaffold_1G00054500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 426
AccB7I CCANNNNNTGG 1 cut(s) 314
AciI CCGC 1 cut(s) 498
AclWI GGATC 6 cut(s) 12, 164, 177, 281, 325, 439
AcoI YGGCCR 3 cut(s) 17, 97, 638
AcuI CTGAAG 1 cut(s) 217
AfaI GTAC 1 cut(s) 57
AfiI CCNNNNNNNGG 4 cut(s) 300, 314, 507, 508
AflII CTTAAG 1 cut(s) 257
AgsI TTSAA 3 cut(s) 185, 474, 575
AjnI CCWGG 4 cut(s) 46, 64, 145, 298
AjuI GAANNNNNNNTTGG 2 cut(s) 396, 428
AluBI AGCT 6 cut(s) 152, 270, 348, 397, 458, 681
AluI AGCT 6 cut(s) 152, 270, 348, 397, 458, 681
AlwI GGATC 6 cut(s) 12, 164, 177, 281, 325, 439
AoxI GGCC 5 cut(s) 17, 97, 302, 483, 638
ApaI GGGCCC 1 cut(s) 487
ApeKI GCWGC 1 cut(s) 365
AspS9I GGNCC 4 cut(s) 302, 416, 483, 484
AsuHPI GGTGA 2 cut(s) 62, 341
AvaII GGWCC 1 cut(s) 416
BaeGI GKGCMC 1 cut(s) 487
BalI TGGCCA 2 cut(s) 99, 640
BamHI GGATCC 1 cut(s) 169
BanII GRGCYC 1 cut(s) 487
BbsI GAAGAC 1 cut(s) 223
BbvI GCAGC 1 cut(s) 352
BccI CCATC 3 cut(s) 9, 233, 538
BcgI CGANNNNNNTGC 2 cut(s) 608, 642
BciT130I CCWGG 4 cut(s) 48, 66, 147, 300
BfaI CTAG 2 cut(s) 77, 536
BfmI CTRYAG 2 cut(s) 369, 384
BfrI CTTAAG 1 cut(s) 257
BisI GCNGC 1 cut(s) 366
BlsI GCNGC 1 cut(s) 367
Bme1390I CCNGG 4 cut(s) 48, 66, 147, 300
Bme18I GGWCC 1 cut(s) 416
BmgT120I GGNCC 4 cut(s) 302, 416, 483, 484
BmiI GGNNCC 2 cut(s) 171, 485
BmrFI CCNGG 4 cut(s) 48, 66, 147, 300
BmsI GCATC 5 cut(s) 581, 603, 616, 643, 719
BpiI GAAGAC 1 cut(s) 223
BpmI CTGGAG 2 cut(s) 282, 756
BsaBI GATNNNNATC 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 299
Bsc4I CCNNNNNNNGG 4 cut(s) 300, 314, 507, 508
Bse118I RCCGGY 1 cut(s) 19
Bse1I ACTGG 1 cut(s) 719
Bse3DI GCAATG 1 cut(s) 147
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 4 cut(s) 48, 66, 147, 300
BseDI CCNNGG 1 cut(s) 299
BseGI GGATG 1 cut(s) 594
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 4 cut(s) 300, 314, 507, 508
BseMI GCAATG 1 cut(s) 147
BseNI ACTGG 1 cut(s) 719
BseSI GKGCMC 1 cut(s) 487
BseXI GCAGC 1 cut(s) 352
BshFI GGCC 5 cut(s) 19, 99, 304, 485, 640
BsiSI CCGG 1 cut(s) 20
BslI CCNNNNNNNGG 4 cut(s) 300, 314, 507, 508
BsnI GGCC 5 cut(s) 19, 99, 304, 485, 640
Bsp120I GGGCCC 1 cut(s) 483
Bsp1286I GDGCHC 1 cut(s) 487
Bsp143I GATC 5 cut(s) 4, 169, 286, 317, 431
BspACI CCGC 1 cut(s) 498
BspANI GGCC 5 cut(s) 19, 99, 304, 485, 640
BspLI GGNNCC 2 cut(s) 171, 485
BspMAI CTGCAG 1 cut(s) 388
BspPI GGATC 6 cut(s) 12, 164, 177, 281, 325, 439
BspQI GCTCTTC 1 cut(s) 260
BspTI CTTAAG 1 cut(s) 257
BsrDI GCAATG 1 cut(s) 147
BsrFI RCCGGY 1 cut(s) 19
BsrI ACTGG 1 cut(s) 719
BssAI RCCGGY 1 cut(s) 19
BssECI CCNNGG 1 cut(s) 299
BssMI GATC 5 cut(s) 4, 169, 286, 317, 431
Bst2UI CCWGG 4 cut(s) 48, 66, 147, 300
Bst4CI ACNGT 2 cut(s) 123, 810
Bst6I CTCTTC 2 cut(s) 260, 277
BstAFI CTTAAG 1 cut(s) 257
BstC8I GCNNGC 1 cut(s) 683
BstDEI CTNAG 1 cut(s) 557
BstF5I GGATG 1 cut(s) 594
BstKTI GATC 5 cut(s) 7, 172, 289, 320, 434
BstMBI GATC 5 cut(s) 4, 169, 286, 317, 431
BstNI CCWGG 4 cut(s) 48, 66, 147, 300
BstSCI CCNGG 4 cut(s) 46, 64, 145, 298
BstSFI CTRYAG 2 cut(s) 369, 384
BstSLI GKGCMC 1 cut(s) 487
BstV1I GCAGC 1 cut(s) 352
BstV2I GAAGAC 1 cut(s) 223
BstX2I RGATCY 2 cut(s) 169, 286
BstXI CCANNNNNNTGG 1 cut(s) 95
BstYI RGATCY 2 cut(s) 169, 286
BsuRI GGCC 5 cut(s) 19, 99, 304, 485, 640
BtsCI GGATG 1 cut(s) 594
Cac8I GCNNGC 1 cut(s) 683
Cfr10I RCCGGY 1 cut(s) 19
Cfr13I GGNCC 4 cut(s) 302, 416, 483, 484
CseI GACGC 3 cut(s) 475, 557, 604
Csp6I GTAC 1 cut(s) 56
CviAII CATG 4 cut(s) 70, 361, 599, 646
CviQI GTAC 1 cut(s) 56
DdeI CTNAG 1 cut(s) 557
DpnI GATC 5 cut(s) 6, 171, 288, 319, 433
DpnII GATC 5 cut(s) 4, 169, 286, 317, 431
EaeI YGGCCR 3 cut(s) 17, 97, 638
Eam1104I CTCTTC 2 cut(s) 260, 277
EarI CTCTTC 2 cut(s) 260, 277
EciI GGCGGA 1 cut(s) 487
Eco24I GRGCYC 1 cut(s) 487
Eco32I GATATC 1 cut(s) 771
Eco47I GGWCC 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 217
EcoO109I RGGNCCY 2 cut(s) 302, 484
EcoRII CCWGG 4 cut(s) 46, 64, 145, 298
EcoRV GATATC 1 cut(s) 771
EcoT22I ATGCAT 1 cut(s) 596
EcoT38I GRGCYC 1 cut(s) 487
FaeI CATG 4 cut(s) 73, 364, 602, 649
FalI AAGNNNNNCTT 2 cut(s) 670, 702
FatI CATG 4 cut(s) 69, 360, 598, 645
Fnu4HI GCNGC 1 cut(s) 366
FokI GGATG 1 cut(s) 581
FriOI GRGCYC 1 cut(s) 487
Fsp4HI GCNGC 1 cut(s) 366
FspBI CTAG 2 cut(s) 77, 536
GluI GCNGC 1 cut(s) 366
GsuI CTGGAG 2 cut(s) 282, 756
HaeIII GGCC 5 cut(s) 19, 99, 304, 485, 640
HapII CCGG 1 cut(s) 20
HgaI GACGC 3 cut(s) 475, 557, 604
Hin1II CATG 4 cut(s) 73, 364, 602, 649
HincII GTYRAC 2 cut(s) 85, 126
HindII GTYRAC 2 cut(s) 85, 126
HindIII AAGCTT 1 cut(s) 679
HinfI GANTC 5 cut(s) 228, 353, 563, 758, 791
HpaI GTTAAC 1 cut(s) 126
HpaII CCGG 1 cut(s) 20
HphI GGTGA 2 cut(s) 62, 341
Hpy166II GTNNAC 2 cut(s) 85, 126
Hpy188I TCNGA 6 cut(s) 236, 401, 652, 763, 775, 790
Hpy188III TCNNGA 5 cut(s) 161, 173, 449, 474, 724
Hpy8I GTNNAC 2 cut(s) 85, 126
Hpy99I CGWCG 1 cut(s) 620
HpyAV CCTTC 2 cut(s) 398, 518
HpyCH4III ACNGT 2 cut(s) 123, 810
HpyCH4V TGCA 4 cut(s) 140, 386, 594, 629
HpyF3I CTNAG 1 cut(s) 557
Hsp92II CATG 4 cut(s) 73, 364, 602, 649
KspAI GTTAAC 1 cut(s) 126
Kzo9I GATC 5 cut(s) 4, 169, 286, 317, 431
LguI GCTCTTC 1 cut(s) 260
LmnI GCTCC 1 cut(s) 149
Lsp1109I GCAGC 1 cut(s) 352
LweI GCATC 5 cut(s) 581, 603, 616, 643, 719
MaeI CTAG 2 cut(s) 77, 536
MaeIII GTNAC 2 cut(s) 108, 117
MalI GATC 5 cut(s) 6, 171, 288, 319, 433
MboI GATC 5 cut(s) 4, 169, 286, 317, 431
MboII GAAGA 5 cut(s) 223, 277, 294, 632, 635
MflI RGATCY 2 cut(s) 169, 286
MhlI GDGCHC 1 cut(s) 487
MlsI TGGCCA 2 cut(s) 99, 640
MluCI AATT 5 cut(s) 192, 214, 376, 539, 581
MluNI TGGCCA 2 cut(s) 99, 640
MlyI GAGTC 4 cut(s) 237, 347, 752, 785
MmeI TCCRAC 1 cut(s) 813
MnlI CCTC 8 cut(s) 197, 306, 315, 320, 470, 501, 505, 743
Mox20I TGGCCA 2 cut(s) 99, 640
Mph1103I ATGCAT 1 cut(s) 596
MscI TGGCCA 2 cut(s) 99, 640
MseI TTAA 6 cut(s) 125, 213, 258, 278, 335, 587
MslI CAYNNNNRTG 1 cut(s) 93
Msp20I TGGCCA 2 cut(s) 99, 640
MspCI CTTAAG 1 cut(s) 257
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 4 cut(s) 48, 66, 147, 300
MvaI CCWGG 4 cut(s) 48, 66, 147, 300
NdeII GATC 5 cut(s) 4, 169, 286, 317, 431
NlaIII CATG 4 cut(s) 73, 364, 602, 649
NlaIV GGNNCC 2 cut(s) 171, 485
NmuCI GTSAC 2 cut(s) 108, 117
NsiI ATGCAT 1 cut(s) 596
PciSI GCTCTTC 1 cut(s) 260
PfeI GAWTC 1 cut(s) 563
PflFI GACNNNGTC 1 cut(s) 356
PflMI CCANNNNNTGG 1 cut(s) 314
PkrI GCNGC 1 cut(s) 367
PleI GAGTC 4 cut(s) 236, 347, 752, 785
PpsI GAGTC 4 cut(s) 236, 347, 752, 785
PsiI TTATAA 1 cut(s) 426
Psp6I CCWGG 4 cut(s) 46, 64, 145, 298
PspGI CCWGG 4 cut(s) 46, 64, 145, 298
PspN4I GGNNCC 2 cut(s) 171, 485
PspOMI GGGCCC 1 cut(s) 483
PspPI GGNCC 4 cut(s) 302, 416, 483, 484
PstI CTGCAG 1 cut(s) 388
PsuI RGATCY 2 cut(s) 169, 286
PsyI GACNNNGTC 1 cut(s) 356
RsaI GTAC 1 cut(s) 57
RsaNI GTAC 1 cut(s) 56
RseI CAYNNNNRTG 1 cut(s) 93
SapI GCTCTTC 1 cut(s) 260
SaqAI TTAA 6 cut(s) 125, 213, 258, 278, 335, 587
SatI GCNGC 1 cut(s) 366
Sau3AI GATC 5 cut(s) 4, 169, 286, 317, 431
Sau96I GGNCC 4 cut(s) 302, 416, 483, 484
SchI GAGTC 4 cut(s) 237, 347, 752, 785
ScrFI CCNGG 4 cut(s) 48, 66, 147, 300
SduI GDGCHC 1 cut(s) 487
SfaNI GCATC 5 cut(s) 581, 603, 616, 643, 719
SfcI CTRYAG 2 cut(s) 369, 384
SinI GGWCC 1 cut(s) 416
SmiMI CAYNNNNRTG 1 cut(s) 93
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 5 cut(s) 192, 214, 376, 539, 581
SsiI CCGC 1 cut(s) 498
SspMI CTAG 2 cut(s) 77, 536
StyD4I CCNGG 4 cut(s) 46, 64, 145, 298
TaaI ACNGT 2 cut(s) 123, 810
TaqI TCGA 2 cut(s) 7, 618
TasI AATT 5 cut(s) 192, 214, 376, 539, 581
TatI WGTACW 1 cut(s) 55
TfiI GAWTC 1 cut(s) 563
Tru1I TTAA 6 cut(s) 125, 213, 258, 278, 335, 587
Tru9I TTAA 6 cut(s) 125, 213, 258, 278, 335, 587
TseFI GTSAC 2 cut(s) 108, 117
TseI GCWGC 1 cut(s) 365
Tsp45I GTSAC 2 cut(s) 108, 117
TspDTI ATGAA 2 cut(s) 86, 690
Tth111I GACNNNGTC 1 cut(s) 356
Van91I CCANNNNNTGG 1 cut(s) 314
Vha464I CTTAAG 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 416
XspI CTAG 2 cut(s) 77, 536
Zsp2I ATGCAT 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.